##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549670_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1169802
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15342852893054	33.0	31.0	34.0	30.0	34.0
2	32.30282304184811	34.0	31.0	34.0	30.0	34.0
3	32.35489852128822	34.0	31.0	34.0	30.0	34.0
4	35.87467622725897	37.0	35.0	37.0	35.0	37.0
5	35.885459248659174	37.0	35.0	37.0	35.0	37.0
6	35.70802409296616	37.0	35.0	37.0	35.0	37.0
7	35.822888830759396	37.0	35.0	37.0	35.0	37.0
8	35.77282309313884	37.0	35.0	37.0	35.0	37.0
9	37.63618885931123	39.0	37.0	39.0	35.0	39.0
10	37.205284313071786	39.0	37.0	39.0	34.0	39.0
11	37.117229240503946	39.0	37.0	39.0	33.0	39.0
12	37.04301240722789	39.0	37.0	39.0	33.0	39.0
13	36.985460787381115	39.0	37.0	39.0	33.0	39.0
14	37.983922920289075	40.0	37.0	41.0	33.0	41.0
15	38.025936867948595	40.0	37.0	41.0	33.0	41.0
16	38.114384314610504	40.0	37.0	41.0	33.0	41.0
17	38.14499376817615	40.0	37.0	41.0	33.0	41.0
18	38.202640275875744	40.0	37.0	41.0	34.0	41.0
19	38.23118185812642	40.0	37.0	41.0	34.0	41.0
20	38.15915171969274	40.0	37.0	41.0	34.0	41.0
21	38.074683578930454	40.0	37.0	41.0	34.0	41.0
22	37.976146390585754	40.0	37.0	41.0	33.0	41.0
23	37.75398058816791	39.0	36.0	41.0	33.0	41.0
24	37.385578072186576	39.0	36.0	41.0	32.0	41.0
25	37.35726729822654	39.0	36.0	41.0	32.0	41.0
26	37.511697706107526	39.0	36.0	41.0	33.0	41.0
27	37.61390132689122	39.0	36.0	41.0	33.0	41.0
28	37.660448520347884	39.0	36.0	41.0	33.0	41.0
29	37.66348322194696	39.0	36.0	41.0	33.0	41.0
30	37.57644028647583	39.0	36.0	41.0	33.0	41.0
31	37.51480079534827	39.0	36.0	41.0	33.0	41.0
32	37.46149946743124	39.0	36.0	41.0	33.0	41.0
33	37.36286824607925	39.0	36.0	41.0	33.0	41.0
34	37.310280714172144	39.0	36.0	41.0	32.0	41.0
35	37.29800256795595	39.0	36.0	41.0	32.0	41.0
36	37.17732231608426	39.0	35.0	41.0	32.0	41.0
37	37.10017763689923	39.0	35.0	41.0	32.0	41.0
38	37.019987143123366	39.0	35.0	41.0	31.0	41.0
39	37.0087835377269	39.0	35.0	41.0	32.0	41.0
40	36.95655076671095	39.0	35.0	41.0	31.0	41.0
41	36.93527964561524	39.0	35.0	41.0	31.0	41.0
42	36.9225244955984	39.0	35.0	41.0	31.0	41.0
43	36.75358223015519	39.0	35.0	41.0	31.0	41.0
44	36.62842942651833	39.0	35.0	40.0	31.0	41.0
45	36.58595557196859	39.0	35.0	40.0	31.0	41.0
46	36.44006934506865	39.0	35.0	40.0	31.0	41.0
47	36.36446509751223	39.0	35.0	40.0	30.0	41.0
48	36.346880925148014	39.0	35.0	40.0	30.0	41.0
49	36.35572772144346	39.0	35.0	40.0	30.0	41.0
50	36.20231714426886	38.0	35.0	40.0	30.0	41.0
51	35.409113679067055	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	9.0
11	7.0
12	5.0
13	9.0
14	7.0
15	17.0
16	49.0
17	90.0
18	182.0
19	318.0
20	592.0
21	994.0
22	1498.0
23	2323.0
24	3521.0
25	5113.0
26	6866.0
27	8838.0
28	10943.0
29	13510.0
30	17737.0
31	23496.0
32	30968.0
33	42373.0
34	68725.0
35	87725.0
36	108300.0
37	159267.0
38	271705.0
39	304458.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.7066614692059	4.481784096795868	56.16830882491225	15.643245609085982
2	19.631527386685953	4.393649523594592	53.22661441850843	22.748208671211025
3	19.49894084639965	4.140187826657845	53.52512647439481	22.835744852547695
4	18.824467730436435	4.44511122395072	51.837404962549215	24.89301608306363
5	16.342252791498048	5.329363430734432	52.30697160716087	26.021412170606652
6	19.77180753666005	6.412367221119472	54.75191528138949	19.063909960830976
7	77.84496863571783	2.004270808222246	12.82028924553044	7.330471310529473
8	77.1329678013886	2.717041003520254	11.347390413078452	8.802600782012684
9	71.67794207908689	5.340134484297343	15.09315251640876	7.888770920207009
10	35.18039805026834	29.176219565362345	20.12545712864228	15.51792525572704
11	23.240856144886056	25.67203680622875	29.073723587410523	22.013383461474678
12	22.19469619645034	21.75068943291258	35.0072063477409	21.04740802289618
13	22.58672835231945	23.578092702867664	34.86530199127716	18.969876953535728
14	18.9031135183561	26.620487911629485	31.964640169874905	22.511758400139513
15	15.335159283365904	26.462170521165117	35.490536005238496	22.712134190230486
16	16.741294680638262	26.819324979782905	31.641423078435494	24.79795726114334
17	16.39687742028138	27.118862850294324	30.094494623876518	26.389765105547774
18	17.57126419684699	24.60279602872965	33.390266045023004	24.43567372940036
19	18.552199432040638	27.159724466191715	31.549783638598665	22.73829246316898
20	21.279926004571713	27.650320310616667	31.15322080146897	19.916532883342654
21	21.315573062791824	28.306499732433355	30.199213200182594	20.17871400459223
22	18.78155448528896	27.07124795478209	28.5280756914418	25.619121868487145
23	19.20880627661775	27.16468257021274	27.188960183005328	26.43755097016418
24	20.628277264015622	25.52944857334831	27.867194619260356	25.975079543375717
25	18.20017404654805	27.086293235949334	25.49730638176375	29.21622633573887
26	17.220435595083615	27.990976250681737	26.74991152348859	28.038676630746057
27	17.832419503471527	29.12339011217283	28.16835669626142	24.87583368809422
28	14.809087349825012	26.160239083195275	28.42113451678147	30.609539050198237
29	16.312589651924	24.300522652551457	28.083385051487348	31.303502644037195
30	19.29762472623572	25.926182379582187	29.44677817271641	25.32941472146568
31	17.39644828782991	28.259739682441985	27.65083321792919	26.692978811798916
32	16.501083089274935	27.293935212967664	27.093473938324603	29.111507759432794
33	17.385848203371168	25.664257711988864	28.098002909894156	28.851891174745813
34	15.043656960750623	22.73376178190839	31.21135029688785	31.011230960453133
35	15.05750545818865	23.19537836317599	28.20297793985649	33.54413823877887
36	16.74984313584692	26.384294094214233	28.626382926341382	28.239479843597465
37	15.621618017408073	27.338558149156867	30.340519164781732	26.69930466865333
38	14.93209962027762	26.214692742874433	29.56739687571059	29.285810761137355
39	17.288139360336192	22.681616205135573	32.862826358648725	27.167418075879507
40	18.79497555996656	21.99397846815102	35.30349580527303	23.907550166609393
41	15.38089351873223	23.47730641595757	31.782729043034635	29.35907102227556
42	17.87071658280632	22.723332666553826	28.320006291663034	31.085944458976815
43	19.274971319932774	22.78291539935818	28.165022798730043	29.777090481979002
44	17.771041595073353	22.080403350310565	27.69648196874343	32.45207308587265
45	18.862850294323312	21.005264138717493	28.086718949018724	32.045166617940474
46	19.290529508412536	22.04219175552786	32.81333080299059	25.853947933069016
47	14.337383591411196	23.77701525557317	34.16843192266725	27.717169230348382
48	14.267371743252276	21.85754512302082	33.64834390777243	30.22673922595448
49	18.4791101400066	19.42662091533439	34.3122169392769	27.782052005382106
50	18.51467171367462	18.739410601110272	31.709297812792247	31.036619872422854
51	16.404998452729608	18.399011114701462	29.655702418016038	35.54028801455289
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	522.0
1	1063.5
2	1605.0
3	2780.0
4	3955.0
5	2789.0
6	1623.0
7	1971.5
8	2320.0
9	3189.5
10	4059.0
11	4815.0
12	5571.0
13	5680.5
14	5790.0
15	5926.0
16	6062.0
17	5718.5
18	5375.0
19	5064.5
20	4754.0
21	4608.5
22	4463.0
23	4780.5
24	5098.0
25	5438.5
26	7230.5
27	8682.0
28	9884.0
29	11086.0
30	12873.5
31	14661.0
32	17041.5
33	19422.0
34	21589.5
35	23757.0
36	26418.0
37	29079.0
38	31801.0
39	34523.0
40	40836.5
41	47150.0
42	55766.0
43	64382.0
44	78000.5
45	91619.0
46	114386.0
47	137153.0
48	162958.5
49	188764.0
50	170676.0
51	152588.0
52	119544.0
53	86500.0
54	70359.0
55	54218.0
56	46186.5
57	38155.0
58	34134.5
59	30114.0
60	27871.0
61	25628.0
62	22079.0
63	18530.0
64	16004.5
65	13479.0
66	10910.5
67	8342.0
68	7268.5
69	6195.0
70	4716.0
71	3237.0
72	2772.0
73	2307.0
74	1960.5
75	1253.0
76	892.0
77	662.5
78	433.0
79	307.0
80	181.0
81	137.5
82	94.0
83	56.0
84	18.0
85	15.0
86	12.0
87	9.0
88	6.0
89	4.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1169802.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.424514928272522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.11788218160353	19.749111262066297
2	13.411185182968097	7.89235237244144
3	5.90846951480659	5.215615483250089
4	3.1178774736437984	3.669681290710262
5	1.961646890004452	2.8860254099467677
6	1.2842897972287743	2.26737625864717
7	0.9352031440415527	1.9262529210972645
8	0.7085743412117017	1.6679565024619665
9	0.5355752620707297	1.4183138064612288
>10	4.39215977561955	28.345489539836798
>50	0.5034147175148692	9.614770851104042
>100	0.10608917022180277	5.83933621071273
>500	0.01057952943877966	2.0643349279025838
>1k	0.005877516354877588	3.3188639411816303
>5k	2.938758177438794E-4	0.8325479280294088
>10k+	8.816274532316383E-4	3.2919712941503523
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	14761	1.261837473350191	TruSeq Adapter, Index 27 (100% over 25bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	12456	1.0647955807905953	TruSeq Adapter, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	10853	0.9277638437957876	TruSeq Adapter, Index 27 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	9628	0.823045267489712	TruSeq Adapter, Index 27 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	4044	0.3456995286381798	TruSeq Adapter, Index 27 (100% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC	3461	0.2958620347716964	No Hit
TTCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	2857	0.24422936531139455	TruSeq Adapter, Index 27 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	2624	0.22431146467521854	TruSeq Adapter, Index 27 (100% over 23bp)
TTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	2602	0.22243080452931352	TruSeq Adapter, Index 27 (100% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2401	0.20524840955990845	No Hit
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	2296	0.19627253159081623	TruSeq Adapter, Index 27 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	1814	0.15506897748507867	TruSeq Adapter, Index 27 (100% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	1810	0.15472703927673231	TruSeq Adapter, Index 27 (100% over 21bp)
TTTCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	1570	0.13421074677595013	TruSeq Adapter, Index 27 (100% over 21bp)
GTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	1568	0.13403977767177694	TruSeq Adapter, Index 27 (95% over 24bp)
TTGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	1484	0.12685907529650317	TruSeq Adapter, Index 27 (95% over 23bp)
GTTCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	1432	0.12241387858800037	TruSeq Adapter, Index 27 (100% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	1401	0.119763857473316	TruSeq Adapter, Index 27 (100% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	1307	0.1117283095771763	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC	1205	0.10300888526434389	No Hit
GTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	1177	0.10061531780591929	TruSeq Adapter, Index 27 (100% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4193820834636974E-4	0.0	0.0	1.3411671376865486	0.0
2	4.2742276043296215E-4	0.0	0.0	4.417328744522577	0.0
3	4.2742276043296215E-4	0.0	0.0	6.088551737815459	0.0
4	4.2742276043296215E-4	0.0	0.0	7.8403011791739114	0.0
5	4.2742276043296215E-4	0.0	0.0	10.413642650636604	0.0
6	4.2742276043296215E-4	0.0	0.0	11.938259637100979	0.0
7	4.2742276043296215E-4	0.0	0.0	13.35841450091554	0.0
8	4.2742276043296215E-4	0.0	0.0	15.737022162724974	0.0
9	4.2742276043296215E-4	0.0	0.0	16.791559597265177	0.0
10	4.2742276043296215E-4	0.0	0.0	18.7583881716735	0.0
11	4.2742276043296215E-4	0.0	0.0	22.763253952378264	0.0
12	4.2742276043296215E-4	0.0	0.0	26.07184805633774	0.0
13	4.2742276043296215E-4	0.0	0.0	27.080052863647012	0.0
14	4.2742276043296215E-4	0.0	0.0	27.39737152099244	0.0
15	4.2742276043296215E-4	0.0	0.0	27.854799359207796	0.0
16	5.129073125195546E-4	0.0	0.0	28.99465037673042	0.0
17	5.129073125195546E-4	0.0	0.0	30.796750219267874	0.0
18	5.129073125195546E-4	0.0	0.0	32.802730718531855	0.0
19	5.129073125195546E-4	0.0	0.0	34.0559342521213	0.0
20	5.129073125195546E-4	0.0	0.0	35.27058425271969	0.0
21	5.129073125195546E-4	0.0	0.0	36.82520631696646	0.0
22	5.129073125195546E-4	0.0	0.0	38.505576157332605	0.0
23	5.129073125195546E-4	0.0	0.0	40.01643013091104	0.0
24	5.129073125195546E-4	0.0	0.0	41.17679744093445	0.0
25	5.129073125195546E-4	0.0	0.0	42.157989129784355	0.0
26	5.129073125195546E-4	0.0	0.0	43.002405535295715	0.0
27	5.129073125195546E-4	0.0	0.0	43.8517800448281	0.0
28	5.98391864606147E-4	0.0	0.0	44.708078803079495	0.0
29	5.98391864606147E-4	0.0	0.0	45.6178908909371	0.0
30	5.98391864606147E-4	0.0	0.0	46.59198736196382	0.0
31	5.98391864606147E-4	0.0	0.0	47.46846047450765	0.0
32	5.98391864606147E-4	0.0	0.0	48.276374976278035	0.0
33	5.98391864606147E-4	0.0	0.0	49.06377318554764	0.0
34	5.98391864606147E-4	0.0	0.0	49.85134236392142	0.0
35	5.98391864606147E-4	0.0	0.0	50.65848750472302	0.0
36	5.98391864606147E-4	0.0	0.0	51.41186286226216	0.0
37	5.98391864606147E-4	0.0	0.0	52.112494251163874	0.0
38	5.98391864606147E-4	0.0	0.0	52.82466605459727	0.0
39	6.838764166927395E-4	0.0	0.0	53.58342693891787	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGT	30	2.1655342E-6	45.000004	20
CGAATAT	205	0.0	45.000004	14
TCGTAAA	30	2.1655342E-6	45.000004	37
TTAGTCG	30	2.1655342E-6	45.000004	1
AACCGTA	20	7.033339E-4	45.0	38
ATTCGAT	20	7.033339E-4	45.0	12
GCGTTAG	55	1.8189894E-12	45.0	1
ACGGGTA	25	3.890848E-5	45.0	5
AGTGCCG	20	7.033339E-4	45.0	11
GTCGTAG	35	1.2119563E-7	45.0	1
AATTCCG	20	7.033339E-4	45.0	11
GCGATAT	25	3.890848E-5	45.0	9
CCGACAC	20	7.033339E-4	45.0	16
TTAAACG	20	7.033339E-4	45.0	43
TTCGGAC	20	7.033339E-4	45.0	13
CCGGTCC	20	7.033339E-4	45.0	11
TTGCACG	35	1.2119563E-7	45.0	1
CGCAATC	25	3.890848E-5	45.0	18
CGCGTCA	20	7.033339E-4	45.0	17
CGATCAA	20	7.033339E-4	45.0	11
>>END_MODULE