SRR3549669_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549669_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	767912
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	2794	0.363843773765744	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2500	0.3255581368698497	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCC	2213	0.28818406275719094	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2176	0.28336580233151715	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2137	0.2782870953963475	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2078	0.2706039233662191	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC	808	0.10522038983633543	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTG	20	7.031822E-4	45.000004	1
CGTTTAA	20	7.031822E-4	45.000004	16
ACTATCC	25	3.8895923E-5	45.0	20
TATTGCG	25	3.8895923E-5	45.0	1
GCTTCGA	25	3.8895923E-5	45.0	42
CGTCCAC	25	3.8895923E-5	45.0	18
TTCGGAT	30	2.1645574E-6	44.999996	12
CGGGTAT	80	0.0	42.187504	6
GCCGATC	60	3.6379788E-12	41.249996	9
GACCGAT	270	0.0	40.833332	9
TTGGGAC	2005	0.0	40.623444	5
TTCGTTG	220	0.0	39.886364	1
GCGATGT	175	0.0	39.857143	9
TCAAGCG	475	0.0	39.789474	17
ACACGAC	455	0.0	39.56044	26
GTCAAGC	490	0.0	39.489796	16
ACTTCGG	40	3.4562254E-7	39.375004	2
CTACGGG	80	0.0	39.375004	3
ATTATCG	40	3.4562254E-7	39.375004	30
CGAGACA	470	0.0	39.255318	22