##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549669_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	767912
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47430695183823	33.0	31.0	34.0	31.0	34.0
2	32.61787418350019	34.0	31.0	34.0	31.0	34.0
3	32.64861077831835	34.0	31.0	34.0	31.0	34.0
4	36.07241845419788	37.0	35.0	37.0	35.0	37.0
5	36.08580931148361	37.0	35.0	37.0	35.0	37.0
6	35.91185839002386	37.0	35.0	37.0	35.0	37.0
7	36.02227989665482	37.0	35.0	37.0	35.0	37.0
8	36.00053912427466	37.0	35.0	37.0	35.0	37.0
9	37.87982086489077	39.0	38.0	39.0	35.0	39.0
10	37.49312421214931	39.0	37.0	39.0	35.0	39.0
11	37.354673452166395	39.0	37.0	39.0	34.0	39.0
12	37.23415703882736	39.0	37.0	39.0	34.0	39.0
13	37.23805592307452	39.0	37.0	39.0	34.0	39.0
14	38.421815260081885	40.0	38.0	41.0	34.0	41.0
15	38.49144433216306	40.0	38.0	41.0	34.0	41.0
16	38.55648563897947	40.0	38.0	41.0	34.0	41.0
17	38.54115836189563	40.0	38.0	41.0	34.0	41.0
18	38.537427465647106	40.0	38.0	41.0	34.0	41.0
19	38.53357155507402	40.0	38.0	41.0	34.0	41.0
20	38.46749106668472	40.0	38.0	41.0	34.0	41.0
21	38.388217660356915	40.0	38.0	41.0	34.0	41.0
22	38.295703153486336	40.0	37.0	41.0	34.0	41.0
23	38.10664893894092	40.0	37.0	41.0	34.0	41.0
24	37.814239131567156	40.0	36.0	41.0	33.0	41.0
25	37.764172196814215	40.0	36.0	41.0	33.0	41.0
26	37.88403098271677	40.0	37.0	41.0	33.0	41.0
27	37.97535134234131	40.0	37.0	41.0	34.0	41.0
28	37.945982091698006	40.0	37.0	41.0	34.0	41.0
29	37.94123284959735	40.0	37.0	41.0	34.0	41.0
30	37.81550229713822	40.0	36.0	41.0	33.0	41.0
31	37.73577180718624	40.0	36.0	41.0	33.0	41.0
32	37.625642000645904	40.0	36.0	41.0	33.0	41.0
33	37.47508308243653	40.0	36.0	41.0	33.0	41.0
34	37.363782568836015	40.0	36.0	41.0	33.0	41.0
35	37.32569747575243	40.0	36.0	41.0	32.0	41.0
36	37.25265915886195	40.0	35.0	41.0	32.0	41.0
37	37.2364619904364	40.0	35.0	41.0	32.0	41.0
38	37.133702820114806	39.0	35.0	41.0	32.0	41.0
39	37.08830048234694	39.0	35.0	41.0	32.0	41.0
40	37.01203262873871	39.0	35.0	41.0	32.0	41.0
41	36.969413161924805	39.0	35.0	41.0	32.0	41.0
42	36.93383747096021	39.0	35.0	41.0	31.0	41.0
43	36.81660398587338	39.0	35.0	41.0	31.0	41.0
44	36.72314926710352	39.0	35.0	41.0	31.0	41.0
45	36.662460021460795	39.0	35.0	41.0	31.0	41.0
46	36.54405322484868	39.0	35.0	41.0	31.0	41.0
47	36.46441910010522	39.0	35.0	41.0	31.0	41.0
48	36.407984248195106	39.0	35.0	41.0	31.0	41.0
49	36.36126926001938	39.0	35.0	41.0	31.0	41.0
50	36.23228442842409	39.0	35.0	40.0	30.0	41.0
51	35.487132640198354	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	4.0
11	7.0
12	6.0
13	6.0
14	17.0
15	24.0
16	48.0
17	81.0
18	156.0
19	253.0
20	453.0
21	751.0
22	1143.0
23	1652.0
24	2465.0
25	3660.0
26	5130.0
27	6352.0
28	7127.0
29	8554.0
30	10416.0
31	13154.0
32	17107.0
33	23514.0
34	40566.0
35	54007.0
36	60741.0
37	91003.0
38	165738.0
39	253557.0
40	214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.956000166685765	4.299060308993739	55.71901467876528	16.02592484555522
2	19.96348539936868	4.642459031764056	53.80694662930128	21.58710893956599
3	20.1295200491723	4.19344924939316	54.23759493275271	21.43943576868183
4	17.436633364239654	4.845346862661347	53.31079602871163	24.407223744387377
5	16.731083770015314	5.952114304764088	53.4509162508204	23.865885674400193
6	20.74716894644178	6.498270635176947	54.68517225932138	18.069388159059894
7	83.59082811572159	2.1554553125879004	9.940071258164998	4.313645313525508
8	85.35808790590589	2.655642834074738	7.724973694902541	4.2612955651168365
9	80.19161049703611	5.305425621685818	9.849175426350936	4.653788454927127
10	36.58713498421694	35.342070445571885	15.582384439883738	12.488410130327434
11	26.447822146287596	25.990608298867578	29.484888893519052	18.076680661325778
12	24.85909843836273	23.85976518142704	31.65232474554376	19.62881163466647
13	23.555563658335853	23.827209367740053	32.83996082884497	19.77726614507912
14	20.10816343539364	25.886559918323975	32.54904207773807	21.456234568544314
15	17.257446165706487	25.691876152475807	35.444816593568014	21.605861088249696
16	20.695730760816346	25.789934263301	32.6767910906458	20.83754388523685
17	20.06063194741064	25.046359478690267	31.54710435570742	23.345904218191667
18	20.958130619133442	23.82799070726854	32.61089812374335	22.60298054985467
19	21.768509933429872	25.83694485826501	31.591770932085968	20.80277427621915
20	25.00572982320891	26.328537644938482	30.560012084718043	18.10572044713457
21	24.051974705434997	26.82546958505662	30.063991707383135	19.058564002125244
22	22.0543499776016	25.058991134400816	29.612377459917283	23.2742814280803
23	21.112705622519247	27.090213461959184	30.00474012647282	21.792340789048744
24	21.439305545427082	25.08842158997385	31.568200522976593	21.904072341622477
25	20.688308035295712	26.771557157590976	28.523190157205512	24.0169446499078
26	19.592739793101295	28.050609965725236	29.190844784298204	23.165805456875265
27	20.552615403848357	27.26861932096386	30.11490899998958	22.063856275198198
28	18.661383075144027	27.21887403765015	31.3275479482024	22.792194939003426
29	19.962183166821198	25.668045296856935	29.947311671128986	24.422459865192884
30	21.48423256831512	26.431934909208348	30.297612226400943	21.78622029607559
31	22.093677400535476	29.33057433664274	26.905426663471854	21.670321599349926
32	21.506500744877016	28.04852639364927	27.392201189719657	23.052771671754055
33	21.084968069257936	28.78767358759858	27.331126483242873	22.796231859900615
34	19.779740386919332	27.578680890518704	29.38813301524133	23.25344570732063
35	19.392195980789463	27.14516767546281	27.886658887997584	25.575977455750138
36	19.554323932950652	31.273114627717757	26.711003344133182	22.46155809519841
37	21.545177051537156	29.643891487566282	27.462391524028796	21.348539936867766
38	20.508990613507798	29.793387784016918	27.411734677931847	22.285886924543437
39	20.47343966496161	28.67450957922262	27.43165883590828	23.42039191990749
40	21.487227703174323	28.428908520768005	28.553271729052287	21.53059204700539
41	19.5732063048891	28.9284449259811	28.003338924251736	23.49500984487806
42	21.265978393357575	28.26352498723812	26.7952577899551	23.675238829449206
43	21.6085957765994	27.41499025930054	27.478278761108044	23.49813520299201
44	20.53555615747638	27.10362645719822	27.44559272416631	24.91522466115909
45	20.58230630593089	26.12330579545573	26.992546020898228	26.301841877715155
46	20.51706445530217	27.8128223025555	27.71749888008001	23.95261436206232
47	18.995535946827243	29.303748346164664	29.4247257498255	22.275989957182592
48	20.102824281948973	27.490129077290103	29.004495306753896	23.402551334007022
49	20.97532008876017	27.21705091208368	28.905004740126472	22.90262425902968
50	20.176530644136307	26.460453801998145	28.203752513308817	25.159263040556727
51	19.555886612007626	25.50122930752482	27.08656721082624	27.85631686964131
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	579.0
1	1300.5
2	2022.0
3	3175.5
4	4329.0
5	3112.5
6	1896.0
7	1984.5
8	2073.0
9	2340.5
10	2608.0
11	2910.5
12	3213.0
13	3301.0
14	3389.0
15	3416.5
16	3444.0
17	3375.5
18	3307.0
19	3664.0
20	4021.0
21	3932.0
22	3843.0
23	4663.5
24	5484.0
25	6340.0
26	9020.5
27	10845.0
28	12275.5
29	13706.0
30	15167.5
31	16629.0
32	19028.0
33	21427.0
34	23355.0
35	25283.0
36	27071.5
37	28860.0
38	30993.5
39	33127.0
40	35125.5
41	37124.0
42	42163.5
43	47203.0
44	49846.0
45	52489.0
46	58466.5
47	64444.0
48	69286.5
49	74129.0
50	71301.5
51	68474.0
52	60403.5
53	52333.0
54	45586.0
55	38839.0
56	35590.5
57	32342.0
58	30399.5
59	28457.0
60	26475.0
61	24493.0
62	21221.0
63	17949.0
64	14597.5
65	11246.0
66	9388.5
67	7531.0
68	5960.0
69	4389.0
70	3717.5
71	3046.0
72	2650.5
73	2255.0
74	1980.5
75	1410.5
76	1115.0
77	839.0
78	563.0
79	450.5
80	338.0
81	222.5
82	107.0
83	71.0
84	35.0
85	23.5
86	12.0
87	9.0
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	767912.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.415489949543463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.77289645728435	21.830078108461553
2	11.044630056160287	6.718556689391376
3	4.141821915756357	3.77926628554459
4	2.1165657041339574	2.57505531606539
5	1.29860302870915	1.9748823684074923
6	0.9191947873308592	1.6774655889440695
7	0.6694877893097185	1.425395938896427
8	0.5962940911178688	1.4509261548294152
9	0.49692874109611923	1.360289801740347
>10	6.476885572069167	43.63179044657366
>50	0.39964843731202687	7.762970326372367
>100	0.06007786961937322	3.1859864723600118
>500	0.00435346881299806	0.7870621998964261
>1k	0.0026120812877988356	1.840274302516913
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	2794	0.363843773765744	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2500	0.3255581368698497	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCC	2213	0.28818406275719094	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2176	0.28336580233151715	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2137	0.2782870953963475	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2078	0.2706039233662191	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC	808	0.10522038983633543	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3932742293387784	0.0
2	0.0	0.0	0.0	1.3650001562679057	0.0
3	0.0	0.0	0.0	1.9261321609767785	0.0
4	0.0	0.0	0.0	2.4953380074800235	0.0
5	0.0	0.0	0.0	3.434377897467418	0.0
6	0.0	0.0	0.0	4.009574013689068	0.0
7	0.0	0.0	0.0	4.572659367219161	0.0
8	0.0	0.0	0.0	5.518991759472439	0.0
9	0.0	0.0	0.0	6.003682713644272	0.0
10	0.0	0.0	0.0	6.937123003677505	0.0
11	0.0	0.0	0.0	8.81611434643553	0.0
12	0.0	0.0	0.0	10.298706101740825	0.0
13	0.0	0.0	0.0	10.758915084020044	0.0
14	0.0	0.0	0.0	10.914271426934336	0.0
15	0.0	0.0	0.0	11.15609601100126	0.0
16	0.0	0.0	0.0	11.74014730854577	0.0
17	0.0	0.0	0.0	12.654575003385805	0.0
18	0.0	0.0	0.0	13.716675869110002	0.0
19	0.0	0.0	0.0	14.396831928658493	0.0
20	0.0	0.0	0.0	15.077248434716479	0.0
21	0.0	0.0	0.0	15.99493171092521	0.0
22	0.0	0.0	0.0	16.96965277271354	0.0
23	0.0	0.0	0.0	17.89514944420715	0.0
24	0.0	0.0	0.0	18.629217931221287	0.0
25	0.0	0.0	0.0	19.228505349571304	0.0
26	0.0	0.0	0.0	19.803441019283458	0.0
27	0.0	0.0	0.0	20.346341768327623	0.0
28	0.0	0.0	0.0	20.926225921720196	0.0
29	0.0	0.0	0.0	21.549995311962828	0.0
30	0.0	0.0	0.0	22.283673129212723	0.0
31	0.0	0.0	0.0	22.88426278011022	0.0
32	0.0	0.0	0.0	23.46844430090948	0.0
33	0.0	0.0	0.0	24.047026221754574	0.0
34	0.0	0.0	0.0	24.625477919344924	0.0
35	0.0	0.0	0.0	25.222421319109483	0.0
36	0.0	0.0	0.0	25.808295742220462	0.0
37	0.0	0.0	0.0	26.37437623060976	0.0
38	0.0	0.0	0.0	26.942930960839263	0.0
39	0.0	0.0	0.0	27.532451635083188	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTG	20	7.031822E-4	45.000004	1
CGTTTAA	20	7.031822E-4	45.000004	16
ACTATCC	25	3.8895923E-5	45.0	20
TATTGCG	25	3.8895923E-5	45.0	1
GCTTCGA	25	3.8895923E-5	45.0	42
CGTCCAC	25	3.8895923E-5	45.0	18
TTCGGAT	30	2.1645574E-6	44.999996	12
CGGGTAT	80	0.0	42.187504	6
GCCGATC	60	3.6379788E-12	41.249996	9
GACCGAT	270	0.0	40.833332	9
TTGGGAC	2005	0.0	40.623444	5
TTCGTTG	220	0.0	39.886364	1
GCGATGT	175	0.0	39.857143	9
TCAAGCG	475	0.0	39.789474	17
ACACGAC	455	0.0	39.56044	26
GTCAAGC	490	0.0	39.489796	16
ACTTCGG	40	3.4562254E-7	39.375004	2
CTACGGG	80	0.0	39.375004	3
ATTATCG	40	3.4562254E-7	39.375004	30
CGAGACA	470	0.0	39.255318	22
>>END_MODULE