FastQCFastQC Report
Sat 18 Jun 2016
SRR3549667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459422
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG85020.5825593968022957No Hit
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC78090.5350748446987917RNA PCR Primer, Index 27 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC72470.4965664489092257RNA PCR Primer, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT68710.47080282467990753RNA PCR Primer, Index 38 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC64040.4388038552248767No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC63920.43798161189840906No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC60280.41304023099555853No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC49810.34129950076126025RNA PCR Primer, Index 38 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47440.3250601950635252No Hit
TCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG22760.15595215092002176RNA PCR Primer, Index 38 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC22140.1517038937332725No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT20280.13895912217302467No Hit
TGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG18730.12833847920615146RNA PCR Primer, Index 27 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT18590.12737919532527262RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC17480.11977344455544729No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTA17060.11689559291281068No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGACTATGT17000.11648447124957689No Hit
TTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG15800.10826203798490086RNA PCR Primer, Index 27 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC15720.1077138757672558No Hit
GAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT15690.10750831493563891RNA PCR Primer, Index 27 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG15240.1044249024613854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA302.1659052E-645.00000445
CGGGTCG207.033914E-445.06
CGAGTTA207.033914E-445.020
CGGTTAT351.2122291E-745.011
CGCATCG253.8913247E-545.021
TACGGAT207.033914E-445.06
CGCCCTA207.033914E-445.025
AATCGCC207.033914E-445.013
TTCCGCG1250.043.1999971
TATTGCG900.042.5000041
TATCCGG700.041.7857132
CGTTTTT31550.040.9350241
CTAGCGG556.184564E-1140.9090922
TTCGATC950.040.26315713
TTTCGCG5600.040.178571
CTCGTTG1850.038.918921
ACGGGAT1450.038.7931065
TTTGGGA105250.038.6935844
CTAGCGA356.2494473E-638.57142617
TAGCGGG3400.038.382353
Produced by FastQC (version 0.11.5)