##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549667_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1459422
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30681598605475	33.0	31.0	34.0	30.0	34.0
2	32.44561545598189	34.0	31.0	34.0	30.0	34.0
3	32.4810061791586	34.0	31.0	34.0	30.0	34.0
4	35.970942606045405	37.0	35.0	37.0	35.0	37.0
5	35.99234628503613	37.0	35.0	37.0	35.0	37.0
6	35.77635392641744	37.0	35.0	37.0	35.0	37.0
7	35.95290464307102	37.0	35.0	37.0	35.0	37.0
8	35.88508121708457	37.0	35.0	37.0	35.0	37.0
9	37.774174296399536	39.0	38.0	39.0	35.0	39.0
10	37.3324048835772	39.0	37.0	39.0	34.0	39.0
11	37.1922117112117	39.0	37.0	39.0	34.0	39.0
12	37.16654744138433	39.0	37.0	39.0	34.0	39.0
13	37.15309417015777	39.0	37.0	39.0	33.0	39.0
14	38.26047914859444	40.0	38.0	41.0	33.0	41.0
15	38.32735288353883	40.0	38.0	41.0	34.0	41.0
16	38.41694999801291	40.0	38.0	41.0	34.0	41.0
17	38.396748164684375	40.0	38.0	41.0	34.0	41.0
18	38.415084190864604	40.0	38.0	41.0	34.0	41.0
19	38.399750038028756	40.0	38.0	41.0	34.0	41.0
20	38.31935108556675	40.0	37.0	41.0	34.0	41.0
21	38.279499692343954	40.0	37.0	41.0	34.0	41.0
22	38.17350087911516	40.0	37.0	41.0	34.0	41.0
23	37.94685361739099	40.0	37.0	41.0	33.0	41.0
24	37.6172758804513	39.0	36.0	41.0	32.0	41.0
25	37.54798612053265	39.0	36.0	41.0	32.0	41.0
26	37.71857009144716	39.0	37.0	41.0	33.0	41.0
27	37.83405896306894	40.0	37.0	41.0	33.0	41.0
28	37.8100933109135	40.0	37.0	41.0	33.0	41.0
29	37.83641811621313	40.0	37.0	41.0	33.0	41.0
30	37.741856707655494	40.0	37.0	41.0	33.0	41.0
31	37.64174104542757	40.0	36.0	41.0	33.0	41.0
32	37.5652525451857	40.0	36.0	41.0	33.0	41.0
33	37.43126936554334	40.0	36.0	41.0	33.0	41.0
34	37.314180545448814	40.0	36.0	41.0	32.0	41.0
35	37.31521177562076	40.0	36.0	41.0	32.0	41.0
36	37.233933022799434	39.0	36.0	41.0	32.0	41.0
37	37.16356955013697	39.0	36.0	41.0	32.0	41.0
38	37.09316428010541	39.0	35.0	41.0	31.0	41.0
39	37.08325967403534	39.0	35.0	41.0	31.0	41.0
40	37.004930033944945	39.0	35.0	41.0	31.0	41.0
41	36.934652211628986	39.0	35.0	41.0	31.0	41.0
42	36.93608428542259	39.0	35.0	41.0	31.0	41.0
43	36.7958541121074	39.0	35.0	41.0	31.0	41.0
44	36.66174074393835	39.0	35.0	41.0	31.0	41.0
45	36.63869805991687	39.0	35.0	41.0	31.0	41.0
46	36.528295448472065	39.0	35.0	40.0	31.0	41.0
47	36.41199735237649	39.0	35.0	40.0	30.0	41.0
48	36.35800063312736	39.0	35.0	40.0	30.0	41.0
49	36.362390727287924	39.0	35.0	40.0	30.0	41.0
50	36.18840678021847	39.0	35.0	40.0	30.0	41.0
51	35.33905066526337	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	14.0
11	5.0
12	11.0
13	12.0
14	18.0
15	27.0
16	45.0
17	106.0
18	203.0
19	399.0
20	791.0
21	1401.0
22	2289.0
23	3135.0
24	4780.0
25	6857.0
26	9345.0
27	11609.0
28	13809.0
29	16655.0
30	21236.0
31	27345.0
32	35418.0
33	48249.0
34	75934.0
35	99477.0
36	127323.0
37	188973.0
38	335427.0
39	428251.0
40	271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.0282111685311	3.726132674442348	58.05544934912589	14.190206807900662
2	19.325390462799657	6.583907875857703	53.900653820485104	20.190047840857545
3	18.37309565019576	6.640574145106762	54.18638337643259	20.79994682826489
4	17.725167908939294	4.046396450101478	54.894197839966786	23.334237800992447
5	18.53130897026357	4.972585037090027	52.301253509951195	24.194852482695204
6	20.21697630979936	7.931359127106484	54.7253638769321	17.126300686162054
7	80.36078666759853	1.7587099550369942	12.907027576670766	4.9734758006936985
8	81.31513708851861	4.113888923149028	9.022270460497376	5.548703527834992
9	76.33008136097715	5.009517466503863	11.257333382667934	7.40306778985105
10	37.0499416892441	32.832244546128535	16.83104681168298	13.286766952944385
11	23.4990975879492	26.68165890331926	30.123021305694998	19.696222203036545
12	21.75779178332244	23.022950181647257	34.47070141466964	20.748556620360663
13	23.13854388929316	22.804233456806873	35.864609413863846	18.192613240036124
14	19.159091750021584	26.399423881509254	32.97072402636112	21.47076034210804
15	16.040048731621148	26.25607946159507	36.26696048161532	21.436911325168456
16	18.623948385045587	26.720783981603674	32.04501508131301	22.610252552037725
17	18.993067118352332	26.75819605295795	31.210164023839575	23.038572804850137
18	18.753725790073055	25.991728232135735	32.822172065379306	22.432373912411897
19	19.586452718953122	26.703585392025065	32.450106960152716	21.2598549288691
20	21.91593658311304	28.19424402263362	31.418054544881468	18.471764849371873
21	22.13266621991446	28.33546431395443	30.694548937867182	18.83732052826393
22	20.41780924228907	26.295204539879485	30.04319518275043	23.243791035081014
23	19.377808474861965	28.055764542401036	29.22739276234016	23.339034220396844
24	20.841401595974297	26.893729161270695	29.121665974611865	23.143203268143143
25	18.17575725184354	29.69456401232817	27.22598398544081	24.90369475038748
26	18.069687862729218	28.97098988503668	28.95399685628968	24.00532539594442
27	19.9000015074461	28.231724614265097	29.69963451284138	22.16863936544742
28	17.137675052178192	28.036510344506247	30.87222201666139	23.953592586654167
29	19.12729834139817	25.11823173831832	29.545326848574298	26.209143071709214
30	21.729492908836512	27.730704347337507	29.29872237091122	21.241080372914755
31	19.774952001545817	30.88606311265693	26.3464577072293	22.992527178567954
32	19.513204542620297	29.662770603704754	26.862141313478897	23.961883540196048
33	20.596510125241362	28.074127976692143	27.46107705653334	23.868284841533153
34	18.61428702595959	27.82587901237613	28.341425578071316	25.218408383592955
35	19.20541145741259	25.069513821225115	27.94921551134627	27.77585921001602
36	21.723120523056387	27.380839811925544	27.687262491589138	23.20877717342893
37	20.217181870630977	29.912595534396498	28.4774383283245	21.39278426664803
38	18.046185407647684	29.439737101400414	28.991545968198366	23.52253152275353
39	19.44050452850512	27.527815806531624	30.002562658367495	23.02911700659576
40	19.390005084204567	26.84103706809956	32.26297808310413	21.505979764591736
41	19.51491754955044	27.995398178182867	28.968591675334483	23.52109259693221
42	21.695849452728545	25.391353563259976	29.44247791248864	23.470319071522834
43	23.049947170866275	25.135224767065317	28.096534107338382	23.718293954730026
44	20.590480340847268	25.51434746084409	27.75441236325066	26.140759835057985
45	20.329692165802626	24.697654276830143	27.808885983629132	27.163767573738095
46	21.444791157047106	26.036746054259837	29.381426345498422	23.137036443194635
47	17.878242208216676	26.91675197441179	32.04179462828435	23.16321118908719
48	18.982514995662665	25.901898148719148	30.902302418354665	24.213284437263518
49	20.60781597098029	24.27680273423314	31.535498300011923	23.579882994774643
50	20.172643690447316	23.596053780195174	29.715120095489862	26.51618243386765
51	18.876103005162317	23.193223070503254	28.205275787263723	29.7253981370707
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	734.0
1	1697.0
2	2660.0
3	5207.5
4	7755.0
5	5351.5
6	2948.0
7	3276.5
8	3605.0
9	4589.0
10	5573.0
11	6287.5
12	7002.0
13	7344.0
14	7686.0
15	7338.0
16	6990.0
17	6942.0
18	6894.0
19	6559.5
20	6225.0
21	6314.5
22	6404.0
23	7313.5
24	8223.0
25	9893.0
26	13971.0
27	16379.0
28	19866.0
29	23353.0
30	27062.0
31	30771.0
32	33522.0
33	36273.0
34	39720.0
35	43167.0
36	47917.5
37	52668.0
38	56281.0
39	59894.0
40	66691.5
41	73489.0
42	82514.0
43	91539.0
44	103234.5
45	114930.0
46	145148.5
47	175367.0
48	177441.5
49	179516.0
50	163999.5
51	148483.0
52	121192.0
53	93901.0
54	78733.0
55	63565.0
56	55498.5
57	47432.0
58	41243.0
59	35054.0
60	31926.5
61	28799.0
62	24480.5
63	20162.0
64	17552.5
65	14943.0
66	12223.0
67	9503.0
68	7942.5
69	6382.0
70	5062.5
71	3743.0
72	3071.5
73	2400.0
74	1924.5
75	1237.0
76	1025.0
77	796.0
78	567.0
79	375.5
80	184.0
81	161.0
82	138.0
83	98.0
84	58.0
85	38.0
86	18.0
87	12.0
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1459422.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.30814622061131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.95834003258489	19.917329665850207
2	13.202841972815909	7.739016461338258
3	5.432374284848315	4.776384595962695
4	2.9799139822076457	3.4934301886154318
5	1.7525981469635041	2.5682701378589403
6	1.1321890268773935	1.9909416929456567
7	0.8290193344738661	1.7007913912131756
8	0.6170984507898849	1.4468809302610126
9	0.5097265384221217	1.344522592854143
>10	4.972304374831418	32.7623497849159
>50	0.5008262263368068	9.520342866756373
>100	0.09881943232695552	5.210414239865042
>500	0.007801533647468658	1.5468416710176685
>1k	0.004491792100057712	2.569870440225994
>5k	0.0016548707737054726	3.412613340319517
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	8502	0.5825593968022957	No Hit
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	7809	0.5350748446987917	RNA PCR Primer, Index 27 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	7247	0.4965664489092257	RNA PCR Primer, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	6871	0.47080282467990753	RNA PCR Primer, Index 38 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC	6404	0.4388038552248767	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	6392	0.43798161189840906	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC	6028	0.41304023099555853	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	4981	0.34129950076126025	RNA PCR Primer, Index 38 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4744	0.3250601950635252	No Hit
TCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	2276	0.15595215092002176	RNA PCR Primer, Index 38 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC	2214	0.1517038937332725	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT	2028	0.13895912217302467	No Hit
TGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	1873	0.12833847920615146	RNA PCR Primer, Index 27 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	1859	0.12737919532527262	RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC	1748	0.11977344455544729	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTA	1706	0.11689559291281068	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGACTATGT	1700	0.11648447124957689	No Hit
TTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	1580	0.10826203798490086	RNA PCR Primer, Index 27 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC	1572	0.1077138757672558	No Hit
GAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	1569	0.10750831493563891	RNA PCR Primer, Index 27 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	1524	0.1044249024613854	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.852027720563346E-5	0.0	0.0	0.4982109355621609	0.0
2	6.852027720563346E-5	0.0	0.0	1.9885269647846888	0.0
3	6.852027720563346E-5	0.0	0.0	2.805151628521428	0.0
4	6.852027720563346E-5	0.0	0.0	3.823637028905964	0.0
5	6.852027720563346E-5	0.0	0.0	5.690334940819037	0.0
6	6.852027720563346E-5	0.0	0.0	6.601380546545139	0.0
7	6.852027720563346E-5	0.0	0.0	7.605065567053258	0.0
8	6.852027720563346E-5	0.0	0.0	9.192680389907785	0.0
9	6.852027720563346E-5	0.0	0.0	9.887544521050115	0.0
10	6.852027720563346E-5	0.0	0.0	11.87799005359656	0.0
11	6.852027720563346E-5	0.0	0.0	14.789005510400694	0.0
12	6.852027720563346E-5	0.0	0.0	17.484387654838695	0.0
13	6.852027720563346E-5	0.0	0.0	18.20837290379342	0.0
14	6.852027720563346E-5	0.0	0.0	18.45662186810943	0.0
15	6.852027720563346E-5	0.0	0.0	18.919270779801867	0.0
16	6.852027720563346E-5	0.0	0.0	19.783722597028138	0.0
17	6.852027720563346E-5	0.0	0.0	21.09698222995131	0.0
18	6.852027720563346E-5	0.0	0.0	22.519257623908643	0.0
19	6.852027720563346E-5	0.0	0.0	23.64586802172367	0.0
20	6.852027720563346E-5	0.0	0.0	24.590008921340093	0.0
21	6.852027720563346E-5	0.0	0.0	25.938693537578576	0.0
22	6.852027720563346E-5	0.0	0.0	27.377071196679232	0.0
23	6.852027720563346E-5	0.0	0.0	28.659770785968693	0.0
24	6.852027720563346E-5	0.0	0.0	29.68750642377599	0.0
25	6.852027720563346E-5	0.0	0.0	30.537089340848638	0.0
26	6.852027720563346E-5	0.0	0.0	31.341174793856744	0.0
27	6.852027720563346E-5	0.0	0.0	32.09434968090107	0.0
28	6.852027720563346E-5	0.0	0.0	32.88911637620921	0.0
29	6.852027720563346E-5	0.0	0.0	33.74596244266566	0.0
30	1.3704055441126692E-4	0.0	0.0	34.67180842826818	0.0
31	1.3704055441126692E-4	0.0	0.0	35.52844893389301	0.0
32	2.055608316169004E-4	0.0	0.0	36.32492863613129	0.0
33	2.055608316169004E-4	0.0	0.0	37.09893368744613	0.0
34	2.055608316169004E-4	0.0	0.0	37.89068549055722	0.0
35	2.7408110882253385E-4	0.0	0.0	38.70004700491016	0.0
36	2.7408110882253385E-4	0.0	0.0	39.47884847562939	0.0
37	2.7408110882253385E-4	0.0	0.0	40.24613853977807	0.0
38	2.7408110882253385E-4	0.0	0.0	40.9956818521305	0.0
39	2.7408110882253385E-4	0.0	0.0	41.748514137788796	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTATA	30	2.1659052E-6	45.000004	45
CGGGTCG	20	7.033914E-4	45.0	6
CGAGTTA	20	7.033914E-4	45.0	20
CGGTTAT	35	1.2122291E-7	45.0	11
CGCATCG	25	3.8913247E-5	45.0	21
TACGGAT	20	7.033914E-4	45.0	6
CGCCCTA	20	7.033914E-4	45.0	25
AATCGCC	20	7.033914E-4	45.0	13
TTCCGCG	125	0.0	43.199997	1
TATTGCG	90	0.0	42.500004	1
TATCCGG	70	0.0	41.785713	2
CGTTTTT	3155	0.0	40.935024	1
CTAGCGG	55	6.184564E-11	40.909092	2
TTCGATC	95	0.0	40.263157	13
TTTCGCG	560	0.0	40.17857	1
CTCGTTG	185	0.0	38.91892	1
ACGGGAT	145	0.0	38.793106	5
TTTGGGA	10525	0.0	38.693584	4
CTAGCGA	35	6.2494473E-6	38.571426	17
TAGCGGG	340	0.0	38.38235	3
>>END_MODULE