SRR3549666_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549666_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	714591
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	3292	0.4606831040413328	No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT	3177	0.44458998224158996	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	2445	0.342153763481488	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2385	0.3337573521077092	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCC	2207	0.3088479983654986	No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	1826	0.2555307861420029	No Hit
TTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCG	1506	0.21074992548184907	No Hit
TTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGG	1226	0.17156667240421444	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGC	785	0.1098530488069399	No Hit
TCCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG	763	0.10677436463655435	No Hit
GCCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG	735	0.10285603932879087	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATAA	45	3.8380676E-10	45.000004	15
ACGAACG	45	3.8380676E-10	45.000004	21
TAGCGAT	25	3.8893188E-5	45.0	10
ATTCGAC	20	7.031494E-4	45.0	10
ATATCGA	20	7.031494E-4	45.0	32
CTCGACC	20	7.031494E-4	45.0	22
TATCGCG	20	7.031494E-4	45.0	1
TGATTCG	90	0.0	42.500004	15
GCTACGA	80	0.0	42.1875	10
AGACACG	125	0.0	41.399998	24
GACCTAT	60	3.6379788E-12	41.249996	33
GATAACG	50	1.0804797E-9	40.5	17
TCCGATA	50	1.0804797E-9	40.5	14
ATTCCGA	50	1.0804797E-9	40.5	12
AACGAAC	50	1.0804797E-9	40.5	20
CGATAAC	50	1.0804797E-9	40.5	16
TGGGCGA	990	0.0	40.454544	6
GCGAGAC	135	0.0	40.0	21
ACGACCA	130	0.0	39.807693	28
CGAATAT	85	0.0	39.705883	14