##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549666_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	714591
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40489454807015	33.0	31.0	34.0	31.0	34.0
2	32.55658271654695	34.0	31.0	34.0	31.0	34.0
3	32.57632967669618	34.0	31.0	34.0	31.0	34.0
4	36.021282104028735	37.0	35.0	37.0	35.0	37.0
5	36.04343743484035	37.0	35.0	37.0	35.0	37.0
6	35.85202164594852	37.0	35.0	37.0	35.0	37.0
7	35.95152331893349	37.0	35.0	37.0	35.0	37.0
8	35.91113378142182	37.0	35.0	37.0	35.0	37.0
9	37.771270558963096	39.0	38.0	39.0	35.0	39.0
10	37.41968762550886	39.0	37.0	39.0	35.0	39.0
11	37.33640502049424	39.0	37.0	39.0	34.0	39.0
12	37.19449027485653	39.0	37.0	39.0	34.0	39.0
13	37.158587219822245	39.0	37.0	39.0	34.0	39.0
14	38.28511414221562	40.0	38.0	41.0	34.0	41.0
15	38.335601763806146	40.0	38.0	41.0	34.0	41.0
16	38.416579553898664	40.0	38.0	41.0	34.0	41.0
17	38.419017312000854	40.0	38.0	41.0	34.0	41.0
18	38.41037460589344	40.0	38.0	41.0	34.0	41.0
19	38.37066377830116	40.0	37.0	41.0	34.0	41.0
20	38.28388126914557	40.0	37.0	41.0	34.0	41.0
21	38.191548732071915	40.0	37.0	41.0	34.0	41.0
22	38.08197276484031	40.0	37.0	41.0	34.0	41.0
23	37.88535539910242	40.0	36.0	41.0	33.0	41.0
24	37.57684465659377	39.0	36.0	41.0	33.0	41.0
25	37.52775643689887	39.0	36.0	41.0	33.0	41.0
26	37.63715328068783	39.0	36.0	41.0	33.0	41.0
27	37.713787327296316	40.0	36.0	41.0	33.0	41.0
28	37.68912286888584	40.0	36.0	41.0	33.0	41.0
29	37.66245166815703	40.0	36.0	41.0	33.0	41.0
30	37.5928454178684	40.0	36.0	41.0	33.0	41.0
31	37.47800630010733	40.0	36.0	41.0	33.0	41.0
32	37.33160787079602	40.0	35.0	41.0	33.0	41.0
33	37.16584731685678	39.0	35.0	41.0	32.0	41.0
34	37.01302003523694	39.0	35.0	41.0	31.0	41.0
35	36.934155342006825	39.0	35.0	41.0	31.0	41.0
36	36.86978285480785	39.0	35.0	41.0	31.0	41.0
37	36.79729103781044	39.0	35.0	41.0	31.0	41.0
38	36.66216199196464	39.0	35.0	41.0	31.0	41.0
39	36.624210212555155	39.0	35.0	41.0	31.0	41.0
40	36.564466946826926	39.0	35.0	41.0	31.0	41.0
41	36.51558303980879	39.0	35.0	41.0	31.0	41.0
42	36.440713638990694	39.0	35.0	41.0	31.0	41.0
43	36.2566517070604	39.0	35.0	40.0	30.0	41.0
44	36.10818216294356	38.0	35.0	40.0	30.0	41.0
45	36.08454206672069	38.0	35.0	40.0	30.0	41.0
46	35.98979136317138	38.0	35.0	40.0	30.0	41.0
47	35.90302984504423	38.0	35.0	40.0	30.0	41.0
48	35.8236879557677	38.0	35.0	40.0	29.0	41.0
49	35.80011782963961	38.0	35.0	40.0	29.0	41.0
50	35.65451146180123	38.0	35.0	40.0	29.0	41.0
51	34.86199518325868	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	10.0
11	10.0
12	12.0
13	12.0
14	10.0
15	29.0
16	57.0
17	77.0
18	133.0
19	318.0
20	539.0
21	832.0
22	1317.0
23	1966.0
24	2833.0
25	4194.0
26	5510.0
27	6816.0
28	7575.0
29	8901.0
30	10912.0
31	13758.0
32	17657.0
33	24761.0
34	41870.0
35	54498.0
36	60909.0
37	90948.0
38	156340.0
39	201646.0
40	135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.197885223855323	4.387824643747262	54.68344829419905	17.730841838198355
2	21.253276349688143	4.477946125825822	53.35401649335074	20.914761031135292
3	19.891798245429904	4.410634894646028	53.97535093501038	21.722215924913694
4	18.36994868393249	4.935830426075896	52.43922747417754	24.25499341581408
5	17.577887211006015	5.490273457124425	51.45950620704711	25.47233312482245
6	21.669318533258885	6.716289457885699	54.39727060654277	17.217121402312653
7	80.84288774977576	2.6777555272876374	11.65939677381887	4.819959949117747
8	81.31322672689693	3.363882276714932	10.025735000860632	5.297155995527512
9	75.1886043904835	6.085019262767093	12.626383483699065	6.099992863050333
10	36.95456561865459	26.531680359814214	20.192249832421624	16.321504189109575
11	27.746641085600015	24.333499862158913	27.41974080278089	20.50011824946018
12	24.861634137569602	21.019016472359713	33.25454700660938	20.86480238346131
13	24.417463975896702	21.58829316350192	33.705574237570865	20.288668623030517
14	21.344937173851896	24.08370662378899	33.42037613124151	21.150980071117605
15	18.235606101952026	24.058937210236344	36.39172617623228	21.31373051157935
16	20.096530742760542	22.98125781041183	34.55920939390504	22.36300205292258
17	20.40999676738162	24.131146348050844	31.586320006829084	23.872536877738455
18	22.080462810194923	22.47649354665816	33.60957526753066	21.83346837561626
19	22.827603482271677	24.089863992129764	31.377809124380242	21.70472340121832
20	25.33477191848204	24.937901540881427	30.183559546649764	19.543766993986768
21	24.382758808885082	25.121363129398492	30.798176859210376	19.69770120250605
22	21.91225470234022	23.911580190626527	30.61583479220981	23.560330314823442
23	22.381893978513585	24.283261334105806	29.16941299288684	24.165431694493776
24	23.028697534673682	23.760864606467198	29.645349577590537	23.565088281268586
25	21.764757742540837	24.235121908896136	28.87315961158201	25.12696073698101
26	20.12018063479669	26.160978797661876	29.997718974910125	23.72112159263131
27	21.371245929489735	25.76788680517947	31.404817580965894	21.456049684364903
28	19.73422559198199	25.0567107618204	32.161054365364244	23.048009280833373
29	21.2231892089321	24.63703013332102	30.347849329196702	23.791931328550177
30	23.637297419083083	23.675920911402468	30.215325969680556	22.471455699833893
31	23.624143041264166	26.462689846359666	27.828646036683924	22.084521075692248
32	23.574604214158867	26.443797920768663	27.437513206855392	22.544084658217077
33	24.76059732070513	24.63940911654359	26.952200629450974	23.64779293330031
34	22.401625545241963	23.948524400671154	30.096096928172898	23.553753125913985
35	22.03428254763914	23.726999080592957	29.533537366129714	24.70518100563819
36	24.152137376485292	25.43986700084384	28.444522810950602	21.963472811720273
37	24.261150784154854	26.62655980833792	28.805008739264835	20.307280668242395
38	22.63658512351821	27.633289532054	27.307928591320067	22.42219675310772
39	21.627896237148242	25.732901757788724	29.011840339438923	23.62736166562411
40	22.28785417112726	23.50141549501743	31.154884402406413	23.0558459314489
41	21.64552870103318	23.83433320598776	29.650947185173056	24.869190907806004
42	24.949236696236028	23.23301021143563	28.386587572471527	23.431165519856815
43	24.759897619757314	23.029537175811058	29.1506610074854	23.059904196946228
44	23.381906573130646	22.918704545677176	28.24930624651024	25.450082634681937
45	22.797096520946948	22.68836299365651	28.24930624651024	26.265234238886297
46	23.121757760733065	23.03863328813265	30.527112712026877	23.312496239107404
47	22.005594808778728	24.469381786224567	31.208201614629903	22.3168217903668
48	22.183738670092403	23.053606888415892	31.450717963142555	23.311936478349153
49	22.823405276584786	22.64666081716674	31.26739631481505	23.26253759143342
50	22.64106320958422	22.13993739075919	29.573840140723856	25.645159258932733
51	22.228379590562994	21.872511688504336	27.605861254899654	28.293247466033016
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	590.0
1	1572.5
2	2555.0
3	3625.5
4	4696.0
5	3442.5
6	2189.0
7	2397.0
8	2605.0
9	2799.0
10	2993.0
11	3227.0
12	3461.0
13	3513.0
14	3565.0
15	3394.0
16	3223.0
17	3336.0
18	3449.0
19	3472.0
20	3495.0
21	3453.0
22	3411.0
23	4187.0
24	4963.0
25	5086.5
26	6711.5
27	8213.0
28	9241.5
29	10270.0
30	11484.0
31	12698.0
32	14108.0
33	15518.0
34	16696.5
35	17875.0
36	19306.5
37	20738.0
38	21667.5
39	22597.0
40	25457.5
41	28318.0
42	30406.5
43	32495.0
44	36901.5
45	41308.0
46	44713.0
47	48118.0
48	53767.5
49	59417.0
50	66234.5
51	73052.0
52	70414.5
53	67777.0
54	58038.0
55	48299.0
56	41503.5
57	34708.0
58	32229.0
59	29750.0
60	27327.5
61	24905.0
62	22789.0
63	20673.0
64	19211.5
65	17750.0
66	14840.0
67	11930.0
68	9906.5
69	7883.0
70	6580.0
71	5277.0
72	4350.0
73	3423.0
74	2869.5
75	1913.0
76	1510.0
77	1106.5
78	703.0
79	510.5
80	318.0
81	257.5
82	197.0
83	150.5
84	104.0
85	64.0
86	24.0
87	18.0
88	12.0
89	10.0
90	8.0
91	5.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	714591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.59916012308149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.71615768413704	22.345711899501257
2	11.527518497680452	7.285198056599768
3	4.490020690544671	4.256426482694098
4	2.3905348549927186	3.0215557465088922
5	1.4660014883812087	2.3162207886016803
6	1.001770856620884	1.8993070622999895
7	0.7411730105223552	1.6394311246881583
8	0.6399973247869191	1.6178702355428516
9	0.5555602005596575	1.5799712161946293
>10	6.096800221000835	40.975083328874824
>50	0.30838308183015145	6.2492161553861125
>100	0.059339018642791085	3.5913141363457823
>500	0.003146766140148012	0.6566982537903074
>1k	0.003596304160169157	2.565995512971688
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	3292	0.4606831040413328	No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT	3177	0.44458998224158996	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	2445	0.342153763481488	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2385	0.3337573521077092	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCC	2207	0.3088479983654986	No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	1826	0.2555307861420029	No Hit
TTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCG	1506	0.21074992548184907	No Hit
TTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGG	1226	0.17156667240421444	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGC	785	0.1098530488069399	No Hit
TCCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG	763	0.10677436463655435	No Hit
GCCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG	735	0.10285603932879087	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3994018956298078E-4	0.0	0.0	0.46432154896997024	0.0
2	1.3994018956298078E-4	0.0	0.0	1.60889235940559	0.0
3	2.7988037912596157E-4	0.0	0.0	2.212454396990726	0.0
4	2.7988037912596157E-4	0.0	0.0	2.881508443291337	0.0
5	2.7988037912596157E-4	0.0	0.0	3.9488322690881916	0.0
6	2.7988037912596157E-4	0.0	0.0	4.653990884296052	0.0
7	2.7988037912596157E-4	0.0	0.0	5.306531988228231	0.0
8	2.7988037912596157E-4	0.0	0.0	6.424374222457322	0.0
9	2.7988037912596157E-4	0.0	0.0	6.992811272462149	0.0
10	2.7988037912596157E-4	0.0	0.0	8.125627106974479	0.0
11	2.7988037912596157E-4	0.0	0.0	10.434780174953225	0.0
12	2.7988037912596157E-4	0.0	0.0	12.249804433585085	0.0
13	2.7988037912596157E-4	0.0	0.0	12.808025849751816	0.0
14	2.7988037912596157E-4	0.0	0.0	13.01667667239022	0.0
15	2.7988037912596157E-4	0.0	0.0	13.336300065352068	0.0
16	2.7988037912596157E-4	0.0	0.0	14.08749900292615	0.0
17	2.7988037912596157E-4	0.0	0.0	15.169376608437554	0.0
18	2.7988037912596157E-4	0.0	0.0	16.446890598958007	0.0
19	2.7988037912596157E-4	0.0	0.0	17.189693125158307	0.0
20	2.7988037912596157E-4	0.0	0.0	18.007363652774803	0.0
21	2.7988037912596157E-4	0.0	0.0	19.136121221789807	0.0
22	2.7988037912596157E-4	0.0	0.0	20.247106386730312	0.0
23	2.7988037912596157E-4	0.0	0.0	21.242920775660483	0.0
24	2.7988037912596157E-4	0.0	0.0	22.0333029663122	0.0
25	2.7988037912596157E-4	0.0	0.0	22.74000092360525	0.0
26	2.7988037912596157E-4	0.0	0.0	23.353218834270233	0.0
27	2.7988037912596157E-4	0.0	0.0	23.970355070242977	0.0
28	2.7988037912596157E-4	0.0	0.0	24.602464906498962	0.0
29	2.7988037912596157E-4	0.0	0.0	25.280195244552477	0.0
30	2.7988037912596157E-4	0.0	0.0	26.012922077104246	0.0
31	2.7988037912596157E-4	0.0	0.0	26.70660559676794	0.0
32	2.7988037912596157E-4	0.0	0.0	27.405326963255906	0.0
33	4.1982056868894235E-4	0.0	0.0	28.059827229841964	0.0
34	4.1982056868894235E-4	0.0	0.0	28.684100415482423	0.0
35	4.1982056868894235E-4	0.0	0.0	29.322367620079177	0.0
36	4.1982056868894235E-4	0.0	0.0	29.95195853292303	0.0
37	5.597607582519231E-4	0.0	0.0	30.61107682576467	0.0
38	5.597607582519231E-4	0.0	0.0	31.267116434435923	0.0
39	6.997009478149039E-4	0.0	0.0	31.93840952376954	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATAA	45	3.8380676E-10	45.000004	15
ACGAACG	45	3.8380676E-10	45.000004	21
TAGCGAT	25	3.8893188E-5	45.0	10
ATTCGAC	20	7.031494E-4	45.0	10
ATATCGA	20	7.031494E-4	45.0	32
CTCGACC	20	7.031494E-4	45.0	22
TATCGCG	20	7.031494E-4	45.0	1
TGATTCG	90	0.0	42.500004	15
GCTACGA	80	0.0	42.1875	10
AGACACG	125	0.0	41.399998	24
GACCTAT	60	3.6379788E-12	41.249996	33
GATAACG	50	1.0804797E-9	40.5	17
TCCGATA	50	1.0804797E-9	40.5	14
ATTCCGA	50	1.0804797E-9	40.5	12
AACGAAC	50	1.0804797E-9	40.5	20
CGATAAC	50	1.0804797E-9	40.5	16
TGGGCGA	990	0.0	40.454544	6
GCGAGAC	135	0.0	40.0	21
ACGACCA	130	0.0	39.807693	28
CGAATAT	85	0.0	39.705883	14
>>END_MODULE