SRR3549665_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549665_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	814986
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	6095	0.7478656075073682	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	5922	0.7266382490005963	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	4996	0.6130166653169502	TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	3771	0.46270733484992377	TruSeq Adapter, Index 23 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCC	2652	0.3254043627743299	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2309	0.2833177502435624	No Hit
TCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	2091	0.25656882449514473	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	1395	0.17116858449102193	TruSeq Adapter, Index 23 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	1284	0.15754871862829545	No Hit
TTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	1269	0.1557081962144135	No Hit
GCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	1185	0.14540127069667455	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC	948	0.11632101655733963	No Hit
TTTCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	900	0.11043134483291737	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	878	0.10773191195922384	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGGT	35	1.2113378E-7	45.000004	40
TATTTCG	20	7.032078E-4	45.0	1
TCGTTAG	45	3.8380676E-10	45.0	1
GGTAATC	25	3.8898026E-5	45.0	8
GCGAATA	25	3.8898026E-5	45.0	30
TAACGAA	20	7.032078E-4	45.0	19
TACGCAC	25	3.8898026E-5	45.0	35
TATCTCG	20	7.032078E-4	45.0	1
CGGATAT	20	7.032078E-4	45.0	6
GCTTACG	25	3.8898026E-5	45.0	1
GCGATCG	20	7.032078E-4	45.0	9
TTACGTA	20	7.032078E-4	45.0	26
ACGTACT	20	7.032078E-4	45.0	28
GGCGATA	55	1.8189894E-12	45.0	8
CGATCGT	20	7.032078E-4	45.0	10
TCGACTA	20	7.032078E-4	45.0	18
TCGGTGA	20	7.032078E-4	45.0	28
AATCGTC	20	7.032078E-4	45.0	20
TACGTAC	20	7.032078E-4	45.0	27
GACTACG	25	3.8898026E-5	45.0	9