##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549665_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	814986
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2984419364259	33.0	31.0	34.0	30.0	34.0
2	32.46053674541648	34.0	31.0	34.0	31.0	34.0
3	32.49276920094333	34.0	31.0	34.0	30.0	34.0
4	35.970109915998556	37.0	35.0	37.0	35.0	37.0
5	35.98444145052798	37.0	35.0	37.0	35.0	37.0
6	35.78826384747713	37.0	35.0	37.0	35.0	37.0
7	35.933223392794474	37.0	35.0	37.0	35.0	37.0
8	35.89677245989502	37.0	35.0	37.0	35.0	37.0
9	37.76171001710459	39.0	38.0	39.0	35.0	39.0
10	37.32741053220546	39.0	37.0	39.0	34.0	39.0
11	37.18410254900084	39.0	37.0	39.0	34.0	39.0
12	37.11783392598155	39.0	37.0	39.0	34.0	39.0
13	37.114208096826204	39.0	37.0	39.0	33.0	39.0
14	38.184800720503176	40.0	38.0	41.0	33.0	41.0
15	38.24156611279212	40.0	38.0	41.0	33.0	41.0
16	38.30693778788838	40.0	38.0	41.0	34.0	41.0
17	38.341181320906124	40.0	38.0	41.0	34.0	41.0
18	38.371695955513346	40.0	38.0	41.0	34.0	41.0
19	38.373747524497354	40.0	37.0	41.0	34.0	41.0
20	38.309969741811514	40.0	37.0	41.0	34.0	41.0
21	38.23927527589431	40.0	37.0	41.0	34.0	41.0
22	38.136066141013465	40.0	37.0	41.0	34.0	41.0
23	37.92279253876754	40.0	37.0	41.0	33.0	41.0
24	37.56434220955943	39.0	36.0	41.0	32.0	41.0
25	37.50693631547045	39.0	36.0	41.0	32.0	41.0
26	37.672848367947424	39.0	36.0	41.0	33.0	41.0
27	37.77450778295578	39.0	36.0	41.0	33.0	41.0
28	37.74921532394421	40.0	36.0	41.0	33.0	41.0
29	37.767710120173845	40.0	36.0	41.0	33.0	41.0
30	37.683979847506585	40.0	36.0	41.0	33.0	41.0
31	37.57702218197613	40.0	36.0	41.0	33.0	41.0
32	37.48946239567305	39.0	36.0	41.0	33.0	41.0
33	37.39673687646168	39.0	36.0	41.0	33.0	41.0
34	37.29421118890386	39.0	36.0	41.0	32.0	41.0
35	37.263827844895495	39.0	36.0	41.0	32.0	41.0
36	37.21291776791258	39.0	35.0	41.0	32.0	41.0
37	37.12388188263357	39.0	35.0	41.0	32.0	41.0
38	37.00726392846012	39.0	35.0	41.0	31.0	41.0
39	37.03375763510048	39.0	35.0	41.0	32.0	41.0
40	37.00538659559796	39.0	35.0	41.0	32.0	41.0
41	36.94738559926183	39.0	35.0	41.0	31.0	41.0
42	36.91128681965089	39.0	35.0	41.0	31.0	41.0
43	36.7974652325316	39.0	35.0	41.0	31.0	41.0
44	36.69082168282645	39.0	35.0	41.0	31.0	41.0
45	36.66499179127003	39.0	35.0	41.0	31.0	41.0
46	36.53184471880498	39.0	35.0	40.0	31.0	41.0
47	36.42846134780229	39.0	35.0	40.0	31.0	41.0
48	36.37432299450543	39.0	35.0	40.0	31.0	41.0
49	36.387195608268115	39.0	35.0	40.0	31.0	41.0
50	36.24659932808662	39.0	35.0	40.0	30.0	41.0
51	35.46394048486722	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	7.0
11	6.0
12	4.0
13	6.0
14	12.0
15	26.0
16	37.0
17	84.0
18	115.0
19	267.0
20	438.0
21	678.0
22	1134.0
23	1701.0
24	2516.0
25	3648.0
26	5045.0
27	6181.0
28	7633.0
29	9230.0
30	11702.0
31	15387.0
32	20149.0
33	27403.0
34	45080.0
35	59583.0
36	71995.0
37	104430.0
38	185337.0
39	234995.0
40	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.24821776079589	4.254797014918048	58.349591281322624	16.147393942963436
2	18.93934374332811	4.112217878589326	55.47788550968974	21.47055286839283
3	18.34632742157534	3.8609252183473086	55.94685062074686	21.84589673933049
4	18.42092993008469	4.1619119837641385	53.263368941306965	24.153789144844207
5	16.935751043576207	5.001190204494311	53.57122699040229	24.49183176152719
6	20.28317050845045	6.5561862412360465	55.10131462380949	18.059328626504016
7	80.54972723457826	1.7918099206612137	11.85075571850314	5.807707126257384
8	81.6160032196872	2.3918202275867313	9.410591102178442	6.581585450547617
9	75.99860611102523	5.209414640251489	12.302051814386996	6.489927434336296
10	36.40406092865399	31.785576684752865	17.695886800509456	14.114475586083689
11	23.61488418205957	25.563752015372042	30.352791336292896	20.468572466275496
12	22.505294569477265	22.16148498256412	34.394823960166185	20.938396487792428
13	22.88873673903601	23.550956703550735	34.68550380006528	18.87480275734798
14	19.515795363355934	26.57775716392674	32.573197576400084	21.333249896317238
15	16.663721830804455	26.19333338241393	35.29005406227837	21.852890724503244
16	17.998100580868872	26.507081103233677	32.21233248178496	23.28248583411249
17	17.64987374016241	26.562787581627166	30.480032785839267	25.307305892371158
18	17.743863084764648	25.079940023509607	34.17837852429367	22.997818367432078
19	18.877256787233154	26.836289212330026	32.38939073800041	21.897063262436408
20	21.239505954703517	28.19631257469453	31.252070587715618	19.312110882886333
21	20.88195380043338	28.823439911851246	30.88580662735311	19.408799660362263
22	19.843776457509698	26.52057826760214	29.22823214141102	24.407413133477142
23	18.55749669319473	27.8325026442172	28.439384234821212	25.170616427766856
24	20.11703268522404	26.43112887828748	29.4322847263634	24.019553710125084
25	18.629645171818904	28.065635483308917	27.267953069132478	26.036766275739705
26	18.1930732552461	27.916675869278734	28.666995506671277	25.223255368803883
27	19.47861681059552	27.14733750027608	30.157082452950114	23.21696323617829
28	16.924339804610142	25.903021646997615	32.58571312881448	24.58692541957776
29	17.86177922074735	24.33109280404817	31.545081756005626	26.262046219198858
30	21.03925711607316	25.216261383631128	30.307146380428623	23.43733511986709
31	19.467941780595005	28.247724500788973	29.227864036928242	23.056469681687783
32	18.192582449269068	29.03718591485007	27.243167367292198	25.52706426858866
33	19.1296537609235	26.653341284390166	27.65421737305917	26.562787581627166
34	17.185080479910084	24.59710964360124	31.12433833219221	27.093471544296467
35	17.030353895649743	25.24436002581639	28.95571212266223	28.769573955871635
36	20.260838836495353	25.407184908697815	28.107967498828202	26.224008755978627
37	19.228919269778867	26.28302817471711	33.01553646320305	21.47251609230097
38	16.37660033423887	28.224411216879798	32.3822740513334	23.016714397547933
39	17.220909316233655	25.530131805945132	31.326059588753672	25.92289928906754
40	19.13492992517663	24.963619006952268	31.397471858412292	24.503979209458812
41	16.994402357831913	27.101815245906064	28.149440603887676	27.754341792374348
42	18.396144228244413	26.425607311045834	27.580228372021114	27.598020088688642
43	20.094823714763198	23.86188229000253	29.69327080465186	26.350023190582416
44	18.643755843658663	23.257086624800916	30.39217851594997	27.70697901559045
45	19.856414711418356	21.99706498025733	29.887016464086503	28.259503844237816
46	20.691152976860952	23.586417435391528	31.439190366460284	24.283239221287236
47	16.340526094926783	25.00386509706915	33.61174302380655	25.04386578419752
48	16.461877872748733	24.58483949417536	33.05431013538883	25.89897249768708
49	19.41456663059243	22.019764756695206	33.18204239091224	25.38362622180013
50	18.78915711435534	21.20245010343736	31.608763831525938	28.39962895068136
51	17.062624388639804	21.41889553930988	29.233876410146927	32.2846036619034
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	475.0
1	886.0
2	1297.0
3	2514.5
4	3732.0
5	2588.0
6	1444.0
7	1693.0
8	1942.0
9	2576.0
10	3210.0
11	3760.5
12	4311.0
13	4519.5
14	4728.0
15	4429.5
16	4131.0
17	4119.5
18	4108.0
19	3863.5
20	3619.0
21	3652.0
22	3685.0
23	4010.0
24	4335.0
25	5053.5
26	7224.0
27	8676.0
28	10232.0
29	11788.0
30	12814.0
31	13840.0
32	15748.0
33	17656.0
34	19725.0
35	21794.0
36	23504.5
37	25215.0
38	27412.0
39	29609.0
40	34783.0
41	39957.0
42	45026.0
43	50095.0
44	57526.5
45	64958.0
46	77983.0
47	91008.0
48	102534.0
49	114060.0
50	103318.5
51	92577.0
52	74543.0
53	56509.0
54	46415.0
55	36321.0
56	30702.5
57	25084.0
58	22403.0
59	19722.0
60	17555.0
61	15388.0
62	13309.5
63	11231.0
64	9684.5
65	8138.0
66	6720.0
67	5302.0
68	4308.0
69	3314.0
70	2920.0
71	2526.0
72	2018.5
73	1511.0
74	1238.5
75	706.5
76	447.0
77	377.5
78	308.0
79	207.0
80	106.0
81	65.0
82	24.0
83	33.5
84	43.0
85	31.0
86	19.0
87	12.0
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	814986.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.17669603649919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.43375530017171	21.33538387629619
2	13.082103604494735	8.157135351906456
3	5.487471349427118	5.132436788102619
4	2.9409562821974036	3.667572002668048
5	1.7577368816249082	2.740021423528186
6	1.122231225827828	2.0992477086181243
7	0.8175415503782495	1.7841771089345684
8	0.625618652141451	1.5603778042062673
9	0.45711957386011803	1.2826330205913847
>10	4.7641268671834425	33.42365659598245
>50	0.4115458346860094	8.247948150998848
>100	0.08941441869433073	5.143704946081136
>500	0.00598757268042393	1.322762989255211
>1k	0.0035925436082543585	2.6074447408858012
>5k	7.98343024056524E-4	1.4954974919446675
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	6095	0.7478656075073682	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	5922	0.7266382490005963	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	4996	0.6130166653169502	TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	3771	0.46270733484992377	TruSeq Adapter, Index 23 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCC	2652	0.3254043627743299	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2309	0.2833177502435624	No Hit
TCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	2091	0.25656882449514473	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	1395	0.17116858449102193	TruSeq Adapter, Index 23 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	1284	0.15754871862829545	No Hit
TTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	1269	0.1557081962144135	No Hit
GCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	1185	0.14540127069667455	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC	948	0.11632101655733963	No Hit
TTTCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	900	0.11043134483291737	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	878	0.10773191195922384	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4540298851759415E-4	0.0	0.0	0.7629578913012003	0.0
2	2.4540298851759415E-4	0.0	0.0	2.7394335608219036	0.0
3	2.4540298851759415E-4	0.0	0.0	3.907306383177134	0.0
4	2.4540298851759415E-4	0.0	0.0	5.150886027490043	0.0
5	2.4540298851759415E-4	0.0	0.0	7.077422188847415	0.0
6	2.4540298851759415E-4	0.0	0.0	8.24897605603041	0.0
7	2.4540298851759415E-4	0.0	0.0	9.393290191487951	0.0
8	2.4540298851759415E-4	0.0	0.0	11.32031715882236	0.0
9	2.4540298851759415E-4	0.0	0.0	12.246099933004984	0.0
10	2.4540298851759415E-4	0.0	0.0	14.014719271251286	0.0
11	2.4540298851759415E-4	0.0	0.0	17.41391876670274	0.0
12	2.4540298851759415E-4	0.0	0.0	20.165254372467746	0.0
13	2.4540298851759415E-4	0.0	0.0	21.005882309634767	0.0
14	2.4540298851759415E-4	0.0	0.0	21.265886775969157	0.0
15	2.4540298851759415E-4	0.0	0.0	21.654850512769546	0.0
16	2.4540298851759415E-4	0.0	0.0	22.61707563074703	0.0
17	2.4540298851759415E-4	0.0	0.0	24.1809061750754	0.0
18	2.4540298851759415E-4	0.0	0.0	26.015784320221453	0.0
19	2.4540298851759415E-4	0.0	0.0	27.147705604758855	0.0
20	2.4540298851759415E-4	0.0	0.0	28.229687381132926	0.0
21	2.4540298851759415E-4	0.0	0.0	29.647871251776102	0.0
22	2.4540298851759415E-4	0.0	0.0	31.09464457058158	0.0
23	2.4540298851759415E-4	0.0	0.0	32.49577293352279	0.0
24	2.4540298851759415E-4	0.0	0.0	33.594196710127534	0.0
25	2.4540298851759415E-4	0.0	0.0	34.53728039500065	0.0
26	2.4540298851759415E-4	0.0	0.0	35.336680630096716	0.0
27	2.4540298851759415E-4	0.0	0.0	36.11227677530657	0.0
28	2.4540298851759415E-4	0.0	0.0	36.935112995806065	0.0
29	2.4540298851759415E-4	0.0	0.0	37.818931858952176	0.0
30	2.4540298851759415E-4	0.0	0.0	38.73146287175485	0.0
31	2.4540298851759415E-4	0.0	0.0	39.529758793402586	0.0
32	2.4540298851759415E-4	0.0	0.0	40.28780862493343	0.0
33	3.6810448277639125E-4	0.0	0.0	41.036533142900616	0.0
34	3.6810448277639125E-4	0.0	0.0	41.78893870569556	0.0
35	3.6810448277639125E-4	0.0	0.0	42.584903298952376	0.0
36	3.6810448277639125E-4	0.0	0.0	43.30859671209076	0.0
37	3.6810448277639125E-4	0.0	0.0	44.02775016994157	0.0
38	3.6810448277639125E-4	0.0	0.0	44.73205674698706	0.0
39	3.6810448277639125E-4	0.0	0.0	45.47292836932168	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGGT	35	1.2113378E-7	45.000004	40
TATTTCG	20	7.032078E-4	45.0	1
TCGTTAG	45	3.8380676E-10	45.0	1
GGTAATC	25	3.8898026E-5	45.0	8
GCGAATA	25	3.8898026E-5	45.0	30
TAACGAA	20	7.032078E-4	45.0	19
TACGCAC	25	3.8898026E-5	45.0	35
TATCTCG	20	7.032078E-4	45.0	1
CGGATAT	20	7.032078E-4	45.0	6
GCTTACG	25	3.8898026E-5	45.0	1
GCGATCG	20	7.032078E-4	45.0	9
TTACGTA	20	7.032078E-4	45.0	26
ACGTACT	20	7.032078E-4	45.0	28
GGCGATA	55	1.8189894E-12	45.0	8
CGATCGT	20	7.032078E-4	45.0	10
TCGACTA	20	7.032078E-4	45.0	18
TCGGTGA	20	7.032078E-4	45.0	28
AATCGTC	20	7.032078E-4	45.0	20
TACGTAC	20	7.032078E-4	45.0	27
GACTACG	25	3.8898026E-5	45.0	9
>>END_MODULE