FastQCFastQC Report
Sat 18 Jun 2016
SRR3549664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460886
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC27650.5999314364072678No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC27470.5960259153022656No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT23750.5153118124655555No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC19100.41441918391966776No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.35909096826547127No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC12830.2783768654287611No Hit
TCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG11850.2571134727459719No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG8710.1889838268031574No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC8370.18160673138259786No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC8110.17596542311981705No Hit
TTCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT7340.15925847172619695No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC7080.1536171634634161No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG6800.1475419084111906No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG6340.13756113225396302No Hit
TTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG6270.13604231849090664No Hit
TGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG5880.12758035609673543No Hit
TTTTTTGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC5100.11065643130839296No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG4880.10588301662450152No Hit
TTTCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC4780.10371328267727811No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCG453.8380676E-1045.0000041
GATGTAC302.1626602E-645.0000049
GCGCGAC600.045.0000049
TACGGGC302.1626602E-645.0000044
AAGCGCA302.1626602E-645.00000412
CGCGACC600.045.00000410
CGTTAGG302.1626602E-645.0000042
CGATGAA302.1626602E-645.00000419
CGGGTTA253.887157E-545.06
CGGGTCA406.7993824E-945.06
CTATGCG207.0288836E-445.01
CCGGGTC253.887157E-545.05
TCGCACA207.0288836E-445.019
AGATCAG551.8189894E-1245.019
CTCGTTC253.887157E-545.015
CTCAACG207.0288836E-445.044
GACACGA351.2098099E-745.025
TAGGTCC207.0288836E-445.019
CAGCACT253.887157E-545.027
TTCACGA207.0288836E-445.027
Produced by FastQC (version 0.11.5)