##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549664_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	460886
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3042509427494	33.0	31.0	34.0	30.0	34.0
2	32.466028475588324	34.0	31.0	34.0	31.0	34.0
3	32.482381760348545	34.0	31.0	34.0	30.0	34.0
4	35.968059346562924	37.0	35.0	37.0	35.0	37.0
5	35.983362480092694	37.0	35.0	37.0	35.0	37.0
6	35.794665058170565	37.0	35.0	37.0	35.0	37.0
7	35.94943435035996	37.0	35.0	37.0	35.0	37.0
8	35.89943934074804	37.0	35.0	37.0	35.0	37.0
9	37.7412266807844	39.0	38.0	39.0	35.0	39.0
10	37.317853004864546	39.0	37.0	39.0	34.0	39.0
11	37.16822815186402	39.0	37.0	39.0	34.0	39.0
12	37.112266373897235	39.0	37.0	39.0	34.0	39.0
13	37.0993998515902	39.0	37.0	39.0	33.0	39.0
14	38.17358522497971	40.0	38.0	41.0	33.0	41.0
15	38.23997908376475	40.0	38.0	41.0	33.0	41.0
16	38.33588132423202	40.0	38.0	41.0	34.0	41.0
17	38.35528742465599	40.0	38.0	41.0	34.0	41.0
18	38.367418407154915	40.0	38.0	41.0	34.0	41.0
19	38.359034555182845	40.0	37.0	41.0	34.0	41.0
20	38.269146817217276	40.0	37.0	41.0	34.0	41.0
21	38.187267567250906	40.0	37.0	41.0	34.0	41.0
22	38.09792226277214	40.0	37.0	41.0	34.0	41.0
23	37.83651488654461	40.0	36.0	41.0	33.0	41.0
24	37.518928758955575	39.0	36.0	41.0	32.0	41.0
25	37.449939898369664	39.0	36.0	41.0	32.0	41.0
26	37.595006140347074	39.0	36.0	41.0	33.0	41.0
27	37.729822559157796	39.0	36.0	41.0	33.0	41.0
28	37.759085760904	39.0	36.0	41.0	33.0	41.0
29	37.750053158481705	40.0	36.0	41.0	33.0	41.0
30	37.69171552184271	40.0	36.0	41.0	33.0	41.0
31	37.59424673346554	40.0	36.0	41.0	33.0	41.0
32	37.47488099009299	39.0	36.0	41.0	33.0	41.0
33	37.37021519421288	39.0	36.0	41.0	33.0	41.0
34	37.26753687462843	39.0	35.0	41.0	32.0	41.0
35	37.200557187677646	39.0	35.0	41.0	32.0	41.0
36	37.14189192121262	39.0	35.0	41.0	32.0	41.0
37	37.144311174563775	39.0	35.0	41.0	32.0	41.0
38	37.071330003514966	39.0	35.0	41.0	32.0	41.0
39	37.017225517807006	39.0	35.0	41.0	31.0	41.0
40	36.924313170719	39.0	35.0	41.0	31.0	41.0
41	36.85626597466619	39.0	35.0	41.0	31.0	41.0
42	36.829864651996374	39.0	35.0	41.0	31.0	41.0
43	36.67197094292298	39.0	35.0	41.0	31.0	41.0
44	36.554032884487704	39.0	35.0	40.0	31.0	41.0
45	36.5392656752429	39.0	35.0	40.0	31.0	41.0
46	36.437756842256	39.0	35.0	40.0	31.0	41.0
47	36.35202631453331	39.0	35.0	40.0	31.0	41.0
48	36.31637541604648	39.0	35.0	40.0	30.0	41.0
49	36.312678189400415	39.0	35.0	40.0	31.0	41.0
50	36.15118489170858	39.0	35.0	40.0	30.0	41.0
51	35.316867945652504	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	2.0
13	0.0
14	0.0
15	9.0
16	21.0
17	32.0
18	59.0
19	130.0
20	232.0
21	397.0
22	640.0
23	1021.0
24	1559.0
25	2245.0
26	3069.0
27	3760.0
28	4433.0
29	5329.0
30	6601.0
31	8684.0
32	11430.0
33	15576.0
34	25679.0
35	34397.0
36	41142.0
37	58940.0
38	103350.0
39	132063.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.08281006582973	3.8888141536084846	61.27328666958858	15.755089110973215
2	19.028566717149143	4.935276836354326	54.937229596906825	21.098926849589702
3	17.45051921733357	4.621316334191102	56.13145116145858	21.796713287016747
4	17.105965466514498	4.039176716151065	55.61765816275608	23.237199654578355
5	16.80957980932378	4.961747590510451	54.56381838458968	23.664854215576085
6	20.20781711746506	6.536106542615744	56.10671619446024	17.149360145458964
7	80.717357437631	1.5648121227375098	12.07435244290345	5.643477996728041
8	81.78855508737519	2.859275395650985	9.301432458351957	6.050737058621872
9	75.07192668035046	5.984560173231558	11.908150822546139	7.035362323871847
10	35.70340604835035	32.15111762995622	18.10729768315809	14.038178638535342
11	23.530981631032404	26.52022409012207	30.254770160082973	19.694024118762556
12	21.29615566539231	22.87311829823427	35.07114557612945	20.759580460243964
13	21.972678710136563	24.790078240606135	35.5465342839661	17.6907087652912
14	18.533867377182208	28.04511310823067	32.465295105514166	20.95572440907296
15	14.61684668225982	27.138598265080738	37.10852575257222	21.136029300087223
16	16.708904154172615	26.73763143163385	32.99362532166305	23.559839092530474
17	16.003957594719736	26.79664819499833	31.080353927001468	26.119040283280466
18	17.104012705961996	24.814379260815038	34.57492742239946	23.5066806108235
19	18.265471287910675	27.219095394522725	33.639772091146185	20.875661226420416
20	20.46514756360575	28.393355406760023	32.23573725389793	18.9057597757363
21	19.542359715851642	29.1167447047643	31.688747325802908	19.652148253581146
22	18.608506224966696	27.07198743290098	29.788711308219384	24.53079503391294
23	17.22681964737484	29.283163298516335	28.483399365569795	25.00661768853903
24	18.387627309139354	27.97589859531424	29.592567359390394	24.04390673615601
25	16.677443011937875	31.142408317892063	27.60856263804932	24.57158603212074
26	15.8967727377269	31.14436107844456	29.103075380896794	23.855790802931747
27	16.39754733274606	30.550070950300075	30.66810447702903	22.38427723992484
28	14.697994731885977	28.500106316963414	33.29738807427433	23.504510876876278
29	15.306171157292692	27.205209097260497	32.444248686226096	25.044371059220715
30	19.246408005450373	27.873053206215854	31.447906857661113	21.43263193067266
31	19.219937251294247	30.61169139440122	27.60465711694432	22.563714237360216
32	18.2695937824104	30.483026171330874	27.16875756694714	24.078622479311587
33	19.654534960923094	27.11581605863489	27.62526958944294	25.60437939099908
34	18.99862438867746	26.417595674418404	29.40597023992918	25.177809696974958
35	16.022617306665857	27.18633241191965	28.745720199789105	28.04533008162539
36	19.333848283523476	27.526980641633724	29.409658787639458	23.729512287203345
37	18.170653914417016	26.46185824694176	32.93308974453553	22.434398094105703
38	17.23897015747929	26.374200995473934	34.10778370356227	22.279045143484506
39	20.112348823787226	25.590493093736843	33.66081851043425	20.636339572041678
40	22.287941052668124	25.250929730996386	30.75749751565463	21.703631700680862
41	20.177223868809207	26.69857622058383	26.13509631448992	26.989103596117044
42	21.776534761307566	24.860377620496173	26.68620873708466	26.676878881111598
43	21.196564877214755	24.62908398172216	29.53333362263119	24.64101751843189
44	18.338808295326828	25.36440681643617	31.17690708765291	25.119877800584096
45	19.30867936973568	24.732146344215273	30.063182652543148	25.8959916335059
46	22.085070928602736	24.32467030892672	31.48522628155336	22.10503248091719
47	17.396709815442428	26.165689563145765	33.385045325742155	23.052555295669645
48	18.128778049235603	24.144365417912457	32.76363352325738	24.96322300959456
49	20.872189652104858	21.67933068047196	34.00276858051666	23.445711086906524
50	20.773900704295638	21.135812326692502	31.295808507960754	26.794478461051106
51	18.914438711525193	21.252327039658397	28.34540428652639	31.487829962290025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	215.0
1	536.0
2	857.0
3	1715.0
4	2573.0
5	1759.0
6	945.0
7	1145.0
8	1345.0
9	1746.5
10	2148.0
11	2438.5
12	2729.0
13	3026.5
14	3324.0
15	3130.5
16	2937.0
17	2628.0
18	2319.0
19	2407.5
20	2496.0
21	2404.0
22	2312.0
23	2624.5
24	2937.0
25	3353.5
26	4473.0
27	5176.0
28	6351.0
29	7526.0
30	8095.0
31	8664.0
32	10021.5
33	11379.0
34	12942.5
35	14506.0
36	15156.0
37	15806.0
38	17406.5
39	19007.0
40	21769.0
41	24531.0
42	27959.5
43	31388.0
44	35764.0
45	40140.0
46	47636.5
47	55133.0
48	58489.5
49	61846.0
50	55419.5
51	48993.0
52	39079.5
53	29166.0
54	23572.5
55	17979.0
56	14705.5
57	11432.0
58	9718.0
59	8004.0
60	7022.0
61	6040.0
62	5523.0
63	5006.0
64	4136.5
65	3267.0
66	2501.5
67	1736.0
68	1476.5
69	1217.0
70	996.5
71	776.0
72	593.0
73	410.0
74	349.5
75	277.0
76	265.0
77	191.0
78	117.0
79	104.0
80	91.0
81	83.0
82	75.0
83	43.5
84	12.0
85	6.0
86	0.0
87	0.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	460886.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.22417709830939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.60995182406127	22.79509190109087
2	12.85164628535613	8.539707443790041
3	5.531620892560622	5.513506565254271
4	2.7590454499057717	3.6666805859981633
5	1.702687186885354	2.8285190320050617
6	1.2322429185672106	2.456415417276878
7	0.8582451947509182	1.9960143240924277
8	0.66537993191468	1.7685360556475502
9	0.557070546805891	1.6657389452997877
>10	4.851754308156894	32.86472464538397
>50	0.2879079531447393	6.282518259628588
>100	0.0812217390752361	5.032859647851648
>500	0.006603393420750902	1.5357461872258733
>1k	0.0046223753945256315	3.0539409894548797
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	2765	0.5999314364072678	No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	2747	0.5960259153022656	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	2375	0.5153118124655555	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC	1910	0.41441918391966776	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1655	0.35909096826547127	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1283	0.2783768654287611	No Hit
TCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	1185	0.2571134727459719	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	871	0.1889838268031574	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	837	0.18160673138259786	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	811	0.17596542311981705	No Hit
TTCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	734	0.15925847172619695	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	708	0.1536171634634161	No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	680	0.1475419084111906	No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	634	0.13756113225396302	No Hit
TTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	627	0.13604231849090664	No Hit
TGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	588	0.12758035609673543	No Hit
TTTTTTGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC	510	0.11065643130839296	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	488	0.10588301662450152	No Hit
TTTCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	478	0.10371328267727811	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5406976996480691	0.0
2	0.0	0.0	0.0	2.1332824169100384	0.0
3	0.0	0.0	0.0	3.2428843575200808	0.0
4	0.0	0.0	0.0	4.50154702030437	0.0
5	0.0	0.0	0.0	6.42718589846513	0.0
6	0.0	0.0	0.0	7.583437118940475	0.0
7	0.0	0.0	0.0	8.76464027980889	0.0
8	0.0	0.0	0.0	10.765134979148856	0.0
9	0.0	0.0	0.0	11.793805843527467	0.0
10	0.0	0.0	0.0	13.824242871339116	0.0
11	0.0	0.0	0.0	17.52190346419722	0.0
12	0.0	0.0	0.0	20.92708392096961	0.0
13	0.0	0.0	0.0	21.90194538345708	0.0
14	0.0	0.0	0.0	22.219811406725306	0.0
15	0.0	0.0	0.0	22.668512387011106	0.0
16	0.0	0.0	0.0	23.761407376227528	0.0
17	0.0	0.0	0.0	25.602426630446576	0.0
18	0.0	0.0	0.0	27.80514053366776	0.0
19	0.0	0.0	0.0	29.125206667158473	0.0
20	0.0	0.0	0.0	30.4153304721775	0.0
21	0.0	0.0	0.0	31.986651796756682	0.0
22	0.0	0.0	0.0	33.416940414766344	0.0
23	0.0	0.0	0.0	34.80730592814709	0.0
24	0.0	0.0	0.0	35.85073098336682	0.0
25	0.0	0.0	0.0	36.72730349804507	0.0
26	0.0	0.0	0.0	37.46175843918019	0.0
27	0.0	0.0	0.0	38.2758426161784	0.0
28	0.0	0.0	0.0	39.11184110604358	0.0
29	0.0	0.0	0.0	39.97734797759099	0.0
30	0.0	0.0	0.0	40.89427754368759	0.0
31	0.0	0.0	0.0	41.71139934821192	0.0
32	0.0	0.0	0.0	42.476013591213444	0.0
33	0.0	0.0	0.0	43.22413785621607	0.0
34	0.0	0.0	0.0	43.950564781746465	0.0
35	0.0	0.0	0.0	44.746423193588	0.0
36	0.0	0.0	0.0	45.482396948486176	0.0
37	0.0	0.0	0.0	46.20513532630629	0.0
38	0.0	0.0	0.0	46.88035653068221	0.0
39	0.0	0.0	0.0	47.61329265805427	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTCG	45	3.8380676E-10	45.000004	1
GATGTAC	30	2.1626602E-6	45.000004	9
GCGCGAC	60	0.0	45.000004	9
TACGGGC	30	2.1626602E-6	45.000004	4
AAGCGCA	30	2.1626602E-6	45.000004	12
CGCGACC	60	0.0	45.000004	10
CGTTAGG	30	2.1626602E-6	45.000004	2
CGATGAA	30	2.1626602E-6	45.000004	19
CGGGTTA	25	3.887157E-5	45.0	6
CGGGTCA	40	6.7993824E-9	45.0	6
CTATGCG	20	7.0288836E-4	45.0	1
CCGGGTC	25	3.887157E-5	45.0	5
TCGCACA	20	7.0288836E-4	45.0	19
AGATCAG	55	1.8189894E-12	45.0	19
CTCGTTC	25	3.887157E-5	45.0	15
CTCAACG	20	7.0288836E-4	45.0	44
GACACGA	35	1.2098099E-7	45.0	25
TAGGTCC	20	7.0288836E-4	45.0	19
CAGCACT	25	3.887157E-5	45.0	27
TTCACGA	20	7.0288836E-4	45.0	27
>>END_MODULE