FastQCFastQC Report
Sat 18 Jun 2016
SRR3549663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167948
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC57270.4903471729905784TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48570.4158575553021196No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC46400.3972779610051132No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT40500.3467620133773079Illumina PCR Primer Index 8 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC37400.32021973581015595TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC23520.2013788285094884TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC17370.14872237462626758No Hit
TCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG14690.12577614756821365No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC13120.11233376828420442No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG12340.10565538876730814No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG11890.10180247750756026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC302.1655324E-645.00000416
GCAAACG207.033334E-445.032
ACGATAG207.033334E-445.025
ACAGGCG501.0822987E-940.533
GGCGATA403.4582808E-739.3758
CCCCGAT1550.039.19354640
TAGGGAC1550.039.1935465
TTTGGGA50000.038.4299964
TTGGGAC17850.037.9411775
CGCCCAA950.037.89473737
TTGGGAT25650.037.8947335
CATACGA1550.037.74193618
ACCGGTA301.139933E-437.50000441
ATCGTTA301.139933E-437.50000415
CAACCCG1150.037.17391623
CGTTTTT27450.036.8852461
ACACGCG2450.036.73469536
GCTTGCG1900.036.7105261
TTTGGGC28900.036.5916984
CGGGAAC2800.036.1607136
Produced by FastQC (version 0.11.5)