##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549663_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1167948
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.364481980362136	33.0	31.0	34.0	31.0	34.0
2	32.5232013754037	34.0	31.0	34.0	31.0	34.0
3	32.52921277317141	34.0	31.0	34.0	31.0	34.0
4	35.995980985454835	37.0	35.0	37.0	35.0	37.0
5	36.00919304626576	37.0	35.0	37.0	35.0	37.0
6	35.81530855825773	37.0	35.0	37.0	35.0	37.0
7	35.92461051348177	37.0	35.0	37.0	35.0	37.0
8	35.90522437642772	37.0	35.0	37.0	35.0	37.0
9	37.76734323788388	39.0	38.0	39.0	35.0	39.0
10	37.40092966467685	39.0	37.0	39.0	35.0	39.0
11	37.287113809861395	39.0	37.0	39.0	34.0	39.0
12	36.93307835622819	39.0	35.0	39.0	33.0	39.0
13	36.7392469527753	39.0	35.0	39.0	33.0	39.0
14	37.340468068783885	40.0	36.0	41.0	31.0	41.0
15	37.54817937099939	40.0	36.0	41.0	32.0	41.0
16	37.81146592142801	40.0	36.0	41.0	33.0	41.0
17	37.907201348005216	40.0	36.0	41.0	33.0	41.0
18	37.95106032117868	40.0	36.0	41.0	33.0	41.0
19	37.94974947514787	40.0	36.0	41.0	33.0	41.0
20	37.80310339158935	40.0	36.0	41.0	33.0	41.0
21	37.73162418189851	40.0	35.0	41.0	33.0	41.0
22	37.73058389585838	40.0	35.0	41.0	33.0	41.0
23	37.566498679735744	39.0	35.0	41.0	33.0	41.0
24	37.24036344083812	39.0	35.0	41.0	32.0	41.0
25	37.16023144866038	39.0	35.0	41.0	32.0	41.0
26	37.29393431899366	39.0	35.0	41.0	33.0	41.0
27	37.37488398456096	39.0	35.0	41.0	33.0	41.0
28	37.362796117635376	39.0	35.0	41.0	33.0	41.0
29	37.32783480086442	39.0	35.0	41.0	33.0	41.0
30	37.214840900451044	39.0	35.0	41.0	32.0	41.0
31	37.01819430317103	39.0	35.0	41.0	32.0	41.0
32	36.908191118097726	39.0	35.0	41.0	31.0	41.0
33	36.76127104973852	39.0	35.0	41.0	31.0	41.0
34	36.5802775466031	39.0	35.0	41.0	31.0	41.0
35	36.49601437735242	39.0	35.0	41.0	31.0	41.0
36	36.38979218252867	39.0	35.0	41.0	31.0	41.0
37	36.3332802487782	39.0	35.0	41.0	30.0	41.0
38	36.24440386044584	38.0	35.0	40.0	30.0	41.0
39	36.204761684595546	38.0	35.0	40.0	30.0	41.0
40	36.05722001321977	38.0	35.0	40.0	30.0	41.0
41	35.8306705435516	38.0	35.0	40.0	29.0	41.0
42	35.789722658885495	38.0	35.0	40.0	29.0	41.0
43	35.71933596358742	38.0	35.0	40.0	29.0	41.0
44	35.64890132094922	38.0	35.0	40.0	29.0	41.0
45	35.60020137882851	38.0	34.0	40.0	29.0	41.0
46	35.44828793747667	37.0	34.0	40.0	29.0	41.0
47	35.25505245096528	37.0	34.0	40.0	28.0	41.0
48	35.074888608054465	36.0	34.0	40.0	28.0	41.0
49	35.00514320843051	36.0	34.0	40.0	28.0	41.0
50	34.76315041423077	36.0	34.0	40.0	27.0	41.0
51	33.79230068461952	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	11.0
10	14.0
11	12.0
12	13.0
13	17.0
14	22.0
15	46.0
16	121.0
17	220.0
18	384.0
19	627.0
20	1018.0
21	1624.0
22	2591.0
23	3642.0
24	5180.0
25	7630.0
26	10188.0
27	12354.0
28	14509.0
29	17167.0
30	21400.0
31	27708.0
32	36162.0
33	54673.0
34	98581.0
35	89962.0
36	99969.0
37	150825.0
38	234410.0
39	276677.0
40	189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.122645871220296	4.245993828492364	56.4388140567902	17.19254624349714
2	22.09516177090076	4.241627195731317	52.640785377431186	21.022425655936736
3	20.09113419433057	4.525972046700709	53.23635983793798	22.14653392103073
4	19.2262840468925	4.596694373379637	51.82576621561918	24.351255364108678
5	17.974087887474443	5.483977026374462	50.885056526489194	25.656878559661905
6	21.924863093219905	6.701325743954355	54.006685229136906	17.36712593368883
7	80.35862898005733	2.6769171230226	11.72963179867597	5.234822098244099
8	81.44737608181187	2.98463630230113	10.098223551048505	5.469764064838503
9	75.63564473760817	5.574563251103645	12.696798145122902	6.0929938661652745
10	41.75588296739238	23.81296085099679	19.794631267830418	14.636524913780407
11	32.93614099257844	21.821433830958227	26.241065526889894	19.00135964957344
12	30.266073489573163	18.79064821379034	31.517413446489055	19.425864850147438
13	29.09667211211458	18.93825752516379	33.07398959542719	18.891080767294437
14	21.360882505043033	21.14041036073524	37.258422464013805	20.24028467020792
15	18.58601581577262	21.424412730703764	39.66563579885406	20.323935654669555
16	24.96883422892115	20.999136947877815	32.89658443697836	21.135444386222673
17	25.83479743961204	20.71111042614911	30.4795247733632	22.974567360875657
18	25.863822704435474	20.40715853788011	32.33560055755907	21.393418200125346
19	26.365985471955945	22.663252131088026	30.525417227479306	20.445345169476724
20	23.661070527112507	28.035152249928935	30.19680670714792	18.106970515810637
21	22.63174387900831	24.27008736690332	34.31351395781319	18.784654796275177
22	20.71265159065301	22.466839277091104	34.13045786285006	22.69005126940583
23	20.420686537414337	23.409518231976083	33.38581854671612	22.78397668389346
24	21.99104754663735	25.371078164438828	29.361495546034583	23.27637874288924
25	23.99687314846209	23.994818262456892	26.81001208957933	25.198296499501687
26	20.895536445115706	23.712956398743778	28.586461041073747	26.805046115066766
27	20.39559980410087	23.409689472476515	32.900009246987025	23.294701476435595
28	20.043186854209264	23.78513426967639	29.733173052224927	26.438505823889418
29	22.801871316188734	25.465602920677977	28.46479466551593	23.267731097617357
30	26.618051488593668	23.011555308969236	28.6449396719717	21.725453530465398
31	25.24256216886368	24.93184628082757	28.215639737385565	21.60995181292318
32	27.626743656395664	24.2473123803457	26.23789757763188	21.888046385626758
33	27.019439221609183	24.258186152123212	25.80953946579814	22.912835160469474
34	23.940021302318254	23.17072335412193	30.061526711805662	22.827728631754155
35	24.23369876056126	25.824180528585178	26.897344744800282	23.044775966053283
36	26.121454037337276	28.00313027634792	24.89948182624569	20.975933860069112
37	25.308318521030042	28.473527931038024	26.214180768321878	20.003972779610052
38	26.855818923445224	25.217732296300866	25.89327607050999	22.033172709743926
39	24.16708620589273	22.69921263617901	26.77405158448835	26.359649573439913
40	26.259645121186903	21.52159171469963	28.647080178227114	23.57168298588636
41	22.21443077945251	23.036642042282704	28.670540126786463	26.07838705147832
42	21.62125368595177	24.95213828012891	30.37892097935867	23.047687054560647
43	21.985225369622622	23.61012647823362	30.83202334350502	23.57262480863874
44	24.10826509399391	22.048156253531836	28.2183795853925	25.625199067081756
45	23.969389048142553	20.861373965279277	26.95787826170343	28.211358724874735
46	23.53135584803433	21.885220917369608	29.391719494361052	25.19170374023501
47	20.72249791942792	24.475319106672558	32.35614941761106	22.446033556288462
48	21.112583779414837	25.780856681975568	29.945425652511926	23.16113388609767
49	25.196926575498225	22.024525064472048	29.84953097226931	22.929017387760414
50	22.894341186422686	22.031631545240028	27.672636110511768	27.401391157825522
51	21.728792720223847	22.81771106247881	26.17787778223003	29.27561843506731
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1169.0
1	2326.5
2	3484.0
3	5829.0
4	8174.0
5	5646.5
6	3119.0
7	3320.5
8	3522.0
9	3906.5
10	4291.0
11	4644.5
12	4998.0
13	4675.0
14	4352.0
15	4295.5
16	4239.0
17	4297.0
18	4355.0
19	4359.5
20	4364.0
21	4884.5
22	5405.0
23	5492.0
24	5579.0
25	6543.0
26	9247.0
27	10987.0
28	11774.0
29	12561.0
30	14367.5
31	16174.0
32	18000.0
33	19826.0
34	23375.0
35	26924.0
36	27904.0
37	28884.0
38	31404.5
39	33925.0
40	36271.0
41	38617.0
42	42702.0
43	46787.0
44	52814.5
45	58842.0
46	66464.5
47	74087.0
48	86698.5
49	99310.0
50	113617.5
51	127925.0
52	126152.5
53	124380.0
54	109888.0
55	95396.0
56	85332.5
57	75269.0
58	66054.0
59	56839.0
60	49112.5
61	41386.0
62	36828.0
63	32270.0
64	29010.5
65	25751.0
66	21795.0
67	17839.0
68	15165.0
69	12491.0
70	11344.0
71	10197.0
72	8549.0
73	6901.0
74	5765.0
75	3606.5
76	2584.0
77	1915.0
78	1246.0
79	917.0
80	588.0
81	480.0
82	372.0
83	328.5
84	285.0
85	164.5
86	44.0
87	38.5
88	33.0
89	28.0
90	23.0
91	15.5
92	8.0
93	9.0
94	10.0
95	5.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1167948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.026240570903767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.41855633441271	21.848230694872278
2	11.637147682682704	7.221138871240977
3	4.644828728420296	4.323347206158394
4	2.4696870701633697	3.0650042069495678
5	1.505307528515687	2.3352016756460143
6	1.044367036763878	1.9441669756174784
7	0.7728929091543588	1.6785972934478164
8	0.6402212405312402	1.5890926581859761
9	0.5303535894990506	1.48093902498959
>10	5.927298144210268	39.63471282520644
>50	0.32351659150998324	6.465908528447891
>100	0.0774637469871078	4.367892994939016
>500	0.004736991717992585	1.0709866475366054
>1k	0.003343758859759472	2.479660883487642
>5k	2.7864657164662263E-4	0.4951195132743089
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	5727	0.4903471729905784	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4857	0.4158575553021196	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC	4640	0.3972779610051132	No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	4050	0.3467620133773079	Illumina PCR Primer Index 8 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	3740	0.32021973581015595	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	2352	0.2013788285094884	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	1737	0.14872237462626758	No Hit
TCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	1469	0.12577614756821365	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC	1312	0.11233376828420442	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	1234	0.10565538876730814	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	1189	0.10180247750756026	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.562025021661923E-5	0.0	0.0	0.3703932024370948	0.0
2	8.562025021661923E-5	0.0	0.0	1.4703565569700021	0.0
3	8.562025021661923E-5	0.0	0.0	2.165678608979167	0.0
4	8.562025021661923E-5	0.0	0.0	2.8835187867953027	0.0
5	8.562025021661923E-5	0.0	0.0	4.0362242154616474	0.0
6	8.562025021661923E-5	0.0	0.0	4.830266415970574	0.0
7	8.562025021661923E-5	0.0	0.0	5.611979300448308	0.0
8	8.562025021661923E-5	0.0	0.0	6.887635408425718	0.0
9	8.562025021661923E-5	0.0	0.0	7.572426169658238	0.0
10	8.562025021661923E-5	0.0	0.0	8.806128355029506	0.0
11	8.562025021661923E-5	0.0	0.0	11.472000465774162	0.0
12	8.562025021661923E-5	0.0	0.0	13.541441913509848	0.0
13	8.562025021661923E-5	0.0	0.0	14.232996674509481	0.0
14	8.562025021661923E-5	0.0	0.0	14.530013322510934	0.0
15	8.562025021661923E-5	0.0	0.0	14.901776448951495	0.0
16	8.562025021661923E-5	0.0	0.0	15.701041484723635	0.0
17	8.562025021661923E-5	0.0	0.0	16.78713435872145	0.0
18	8.562025021661923E-5	0.0	0.0	18.043697150900552	0.0
19	8.562025021661923E-5	0.0	0.0	18.862483603722083	0.0
20	8.562025021661923E-5	0.0	0.0	19.80824488761486	0.0
21	8.562025021661923E-5	0.0	0.0	21.001876795884748	0.0
22	8.562025021661923E-5	0.0	0.0	22.204498830427383	0.0
23	8.562025021661923E-5	0.0	0.0	23.25154887032642	0.0
24	8.562025021661923E-5	0.0	0.0	24.050813906098558	0.0
25	8.562025021661923E-5	0.0	0.0	24.77002400791816	0.0
26	8.562025021661923E-5	0.0	0.0	25.451133098391367	0.0
27	8.562025021661923E-5	0.0	0.0	26.332507954121244	0.0
28	8.562025021661923E-5	0.0	0.0	27.067643422481137	0.0
29	8.562025021661923E-5	0.0	0.0	27.94079873419022	0.0
30	8.562025021661923E-5	0.0	0.0	28.726450150177918	0.0
31	8.562025021661923E-5	0.0	0.0	29.401737063636396	0.0
32	8.562025021661923E-5	0.0	0.0	30.066064585067142	0.0
33	8.562025021661923E-5	0.0	0.0	30.753423953806163	0.0
34	8.562025021661923E-5	0.0	0.0	31.503714206454397	0.0
35	8.562025021661923E-5	0.0	0.0	32.29690020446116	0.0
36	8.562025021661923E-5	0.0	0.0	32.966279320654685	0.0
37	8.562025021661923E-5	0.0	0.0	33.6943083082466	0.0
38	8.562025021661923E-5	0.0	0.0	34.57362827797128	0.0
39	1.7124050043323846E-4	0.0	0.0	36.31625723063013	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATAAC	30	2.1655324E-6	45.000004	16
GCAAACG	20	7.033334E-4	45.0	32
ACGATAG	20	7.033334E-4	45.0	25
ACAGGCG	50	1.0822987E-9	40.5	33
GGCGATA	40	3.4582808E-7	39.375	8
CCCCGAT	155	0.0	39.193546	40
TAGGGAC	155	0.0	39.193546	5
TTTGGGA	5000	0.0	38.429996	4
TTGGGAC	1785	0.0	37.941177	5
CGCCCAA	95	0.0	37.894737	37
TTGGGAT	2565	0.0	37.894733	5
CATACGA	155	0.0	37.741936	18
ACCGGTA	30	1.139933E-4	37.500004	41
ATCGTTA	30	1.139933E-4	37.500004	15
CAACCCG	115	0.0	37.173916	23
CGTTTTT	2745	0.0	36.885246	1
ACACGCG	245	0.0	36.734695	36
GCTTGCG	190	0.0	36.710526	1
TTTGGGC	2890	0.0	36.591698	4
CGGGAAC	280	0.0	36.160713	6
>>END_MODULE