##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549662_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1501547
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44104247153103	33.0	31.0	34.0	31.0	34.0
2	32.588483077785774	34.0	31.0	34.0	31.0	34.0
3	32.61641227347529	34.0	31.0	34.0	31.0	34.0
4	36.046724478154864	37.0	35.0	37.0	35.0	37.0
5	36.074529135618135	37.0	35.0	37.0	35.0	37.0
6	35.888817333057176	37.0	35.0	37.0	35.0	37.0
7	35.988992019563824	37.0	35.0	37.0	35.0	37.0
8	35.96785515205318	37.0	35.0	37.0	35.0	37.0
9	37.839160545757146	39.0	38.0	39.0	35.0	39.0
10	37.45875753472918	39.0	37.0	39.0	35.0	39.0
11	37.3331384232395	39.0	37.0	39.0	34.0	39.0
12	37.247123799654624	39.0	37.0	39.0	34.0	39.0
13	37.26822537023483	39.0	37.0	39.0	34.0	39.0
14	38.45251397392156	40.0	38.0	41.0	34.0	41.0
15	38.51176886237993	40.0	38.0	41.0	34.0	41.0
16	38.57075802489033	40.0	38.0	41.0	34.0	41.0
17	38.557695496711055	40.0	38.0	41.0	34.0	41.0
18	38.537355141064516	40.0	38.0	41.0	34.0	41.0
19	38.51653128406903	40.0	38.0	41.0	34.0	41.0
20	38.44440700157904	40.0	38.0	41.0	34.0	41.0
21	38.360050667744666	40.0	37.0	41.0	34.0	41.0
22	38.26114267485467	40.0	37.0	41.0	34.0	41.0
23	38.06654803346149	40.0	37.0	41.0	34.0	41.0
24	37.78089730124998	40.0	36.0	41.0	33.0	41.0
25	37.72660329646691	40.0	36.0	41.0	33.0	41.0
26	37.83067196697806	40.0	37.0	41.0	33.0	41.0
27	37.9247642597934	40.0	37.0	41.0	33.0	41.0
28	37.88626396642929	40.0	37.0	41.0	33.0	41.0
29	37.87168500220106	40.0	37.0	41.0	33.0	41.0
30	37.785241487612446	40.0	36.0	41.0	33.0	41.0
31	37.68793650814793	40.0	36.0	41.0	33.0	41.0
32	37.566748826377065	40.0	36.0	41.0	33.0	41.0
33	37.43463308174836	40.0	36.0	41.0	33.0	41.0
34	37.33038126678685	40.0	36.0	41.0	32.0	41.0
35	37.259235974631494	40.0	36.0	41.0	32.0	41.0
36	37.189003740808644	40.0	35.0	41.0	32.0	41.0
37	37.16120108128484	40.0	35.0	41.0	32.0	41.0
38	37.013659912077344	39.0	35.0	41.0	31.0	41.0
39	36.96117470848398	39.0	35.0	41.0	31.0	41.0
40	36.87935908766092	39.0	35.0	41.0	31.0	41.0
41	36.80271080425721	39.0	35.0	41.0	31.0	41.0
42	36.75552680002691	39.0	35.0	41.0	31.0	41.0
43	36.65530416297325	39.0	35.0	41.0	31.0	41.0
44	36.551679035021884	39.0	35.0	41.0	31.0	41.0
45	36.469999274082	39.0	35.0	41.0	31.0	41.0
46	36.349678698036094	39.0	35.0	41.0	30.0	41.0
47	36.25106706616576	39.0	35.0	41.0	30.0	41.0
48	36.15690817536847	38.0	35.0	40.0	30.0	41.0
49	36.085205458104205	38.0	35.0	40.0	30.0	41.0
50	35.90627399608537	38.0	35.0	40.0	29.0	41.0
51	35.02936105230139	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	7.0
10	17.0
11	18.0
12	15.0
13	14.0
14	33.0
15	45.0
16	78.0
17	164.0
18	274.0
19	558.0
20	1025.0
21	1662.0
22	2549.0
23	3517.0
24	5254.0
25	7505.0
26	10545.0
27	13466.0
28	15191.0
29	17754.0
30	21509.0
31	26731.0
32	34419.0
33	47056.0
34	79672.0
35	103868.0
36	121849.0
37	184337.0
38	320872.0
39	481242.0
40	294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.278525414122903	4.038634821287646	54.716635576508764	17.96620418808069
2	20.2509145567871	4.124013434144919	54.380848551527194	21.24422345754079
3	19.807638388941537	4.300098498415301	54.03100935235461	21.861253760288555
4	17.943028090362805	4.569620531358659	53.50375312927268	23.98359824900586
5	17.07019493895296	5.256978303043462	53.522800152109795	24.15002660589379
6	21.059014469743538	6.540854199036062	55.049891878176304	17.350239453044093
7	82.28427082202555	2.3378555583008724	10.778084202492497	4.59978941718108
8	84.22207230276508	2.7568900607173803	8.691969015954879	4.329068620562659
9	78.84348608468467	5.2128904389939175	10.818375981571005	5.125247494750415
10	38.03836976131949	30.76267342946974	16.938131140750173	14.260825668460594
11	25.86066237020886	25.37303194638596	29.152600617896073	19.613705065509105
12	23.58667427659607	22.27775753939104	33.49725316623456	20.638315017778332
13	23.63216069826652	21.536255608382554	34.204257342594005	20.62732635075692
14	20.597290660898395	23.84840434565152	33.31743861497509	22.236866378475
15	18.210219193937984	24.708916870400994	35.22467162200051	21.85619231366051
16	21.199802603581507	23.761693773155283	33.35659822836048	21.681905394902724
17	20.634652128771194	23.79073049328459	32.231425323349846	23.34319205459436
18	21.000674637557132	23.236835077423486	33.02460728834995	22.737882996669434
19	21.607182459157123	24.956128579391788	31.867933537877935	21.568755423573155
20	24.438728857638157	25.129150136492562	30.744092592506263	19.688028413363018
21	24.549081713725908	25.010339336697417	30.411568868640142	20.029010080936526
22	23.008270803378117	24.15029299782158	29.602736377882277	23.238699820918026
23	21.67251507944806	25.385219377082436	29.546993866991844	23.39527167647766
24	21.758293280197023	24.409492343563006	30.904327337073035	22.92788703916694
25	21.879168617432555	25.852137828519524	28.307938412850213	23.960755141197712
26	21.484508976408996	26.462508333072492	28.902724989627366	23.150257700891146
27	21.544447160162154	25.407529701035	29.86366727115435	23.1843558676485
28	20.56292610221325	25.220256175797363	31.477869157608783	22.7389485643806
29	21.703216749126067	23.767221405656965	30.740363105517176	23.789198739699792
30	22.844506365768105	23.839746607998286	30.516527288190115	22.799219738043497
31	22.155483644534606	26.361545792439394	28.67862278037251	22.80434778265349
32	22.12444898494686	26.580786349012055	28.55801383506477	22.73675083097632
33	21.52153745437206	26.561672728192992	28.244736927981606	23.672052889453344
34	21.547777059259552	25.193550385036232	29.637234132531315	23.6214384231729
35	21.60598369548206	24.62866630215371	29.54479613358756	24.22055386877667
36	21.755695958901054	26.92536430761075	27.810984271554602	23.50795546193359
37	21.859255820830118	26.93209070378749	29.274807914770566	21.93384556061182
38	20.709508260480693	26.98124001446508	29.917478440568296	22.391773284485932
39	20.241590839314387	25.53166833938598	30.490420879266516	23.736319942033116
40	21.011729902560493	25.042839151888018	31.205150421531926	22.74028052401956
41	18.8971773777311	27.157391676717413	30.043415224431868	23.902015721119618
42	21.13074049630148	27.39701121576614	28.15456326042408	23.317685027508297
43	21.589001210085332	25.22944669730618	29.654749401783626	23.526802690824862
44	21.48137887125744	24.24812543330312	29.457153189344055	24.81334250609538
45	21.873907376858668	23.30389924524507	28.960998223831822	25.861195154064443
46	21.802447742228516	24.067644902224174	29.971089816036393	24.158817539510917
47	19.613172281653522	25.93958097881718	31.058967851156172	23.388278888373122
48	19.935439916299657	24.869817594787243	30.969127173508387	24.225615315404713
49	21.130340908409792	24.664629212405607	30.771197971159076	23.433831908025525
50	20.54401227534003	23.650142153392466	30.02223706617242	25.783608505095078
51	19.274787935375983	23.653871640381553	29.206345189328076	27.864995234914392
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	928.0
1	2641.0
2	4354.0
3	7509.0
4	10664.0
5	7426.0
6	4188.0
7	4305.0
8	4422.0
9	4813.0
10	5204.0
11	5194.0
12	5184.0
13	5527.5
14	5871.0
15	5519.5
16	5168.0
17	5387.5
18	5607.0
19	5819.0
20	6031.0
21	6729.5
22	7428.0
23	8647.0
24	9866.0
25	11593.5
26	15513.5
27	17706.0
28	21197.0
29	24688.0
30	28022.0
31	31356.0
32	35008.5
33	38661.0
34	42716.5
35	46772.0
36	49642.5
37	52513.0
38	57220.5
39	61928.0
40	66571.0
41	71214.0
42	78191.0
43	85168.0
44	91360.5
45	97553.0
46	109963.5
47	122374.0
48	132904.0
49	143434.0
50	136382.5
51	129331.0
52	113918.0
53	98505.0
54	88993.5
55	79482.0
56	73365.5
57	67249.0
58	64283.5
59	61318.0
60	55554.0
61	49790.0
62	44790.0
63	39790.0
64	34164.0
65	28538.0
66	24484.0
67	20430.0
68	17754.0
69	15078.0
70	12931.5
71	10785.0
72	9349.0
73	7913.0
74	6696.0
75	4179.0
76	2879.0
77	2341.5
78	1804.0
79	1354.5
80	905.0
81	653.0
82	401.0
83	264.5
84	128.0
85	104.5
86	81.0
87	55.0
88	29.0
89	20.5
90	12.0
91	14.0
92	16.0
93	8.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1501547.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.447146019960694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.074634065538	20.218704935776323
2	11.521989740359464	6.555354491689894
3	4.2838456112642875	3.6558954489180886
4	2.209342775159691	2.5139798613245166
5	1.3185679313550522	1.8754747240247343
6	0.874847173494563	1.493214317372983
7	0.6600234263888466	1.3143047950954807
8	0.5255913750529289	1.1961259674370077
9	0.4291634371960974	1.0987627467911052
>10	6.5031320657589555	43.45588753327627
>50	0.5146191767431098	9.336930915825919
>100	0.0763900404854425	3.7345810888746307
>500	0.004521528571705792	0.8235358202276156
>1k	0.0026177270678296688	1.550061625626939
>5k	7.13925563953546E-4	1.1771857277384306
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6180	0.4115755284383372	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	6052	0.40305098674899953	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCC	5157	0.34344579290558336	No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	4538	0.3022216420798017	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	4516	0.30075648647694675	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	3192	0.2125807583778596	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGC	1820	0.12120832714527086	No Hit
TCCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG	1701	0.11328316729346467	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.659798194795101E-5	0.0	0.0	0.4266266723585742	0.0
2	6.659798194795101E-5	0.0	0.0	1.317374680912419	0.0
3	6.659798194795101E-5	0.0	0.0	1.8578839024019893	0.0
4	6.659798194795101E-5	0.0	0.0	2.4340896422156617	0.0
5	6.659798194795101E-5	0.0	0.0	3.3288335296863836	0.0
6	6.659798194795101E-5	0.0	0.0	3.940203003968574	0.0
7	6.659798194795101E-5	0.0	0.0	4.5550355733120576	0.0
8	6.659798194795101E-5	0.0	0.0	5.553738910603531	0.0
9	6.659798194795101E-5	0.0	0.0	6.0439000577404505	0.0
10	6.659798194795101E-5	0.0	0.0	6.988326039744344	0.0
11	6.659798194795101E-5	0.0	0.0	8.802521665988477	0.0
12	6.659798194795101E-5	0.0	0.0	10.276335006496634	0.0
13	6.659798194795101E-5	0.0	0.0	10.710886838707014	0.0
14	6.659798194795101E-5	0.0	0.0	10.868257870050021	0.0
15	6.659798194795101E-5	0.0	0.0	11.105080293856936	0.0
16	6.659798194795101E-5	0.0	0.0	11.632536310884708	0.0
17	1.3319596389590202E-4	0.0	0.0	12.459883040624103	0.0
18	1.3319596389590202E-4	0.0	0.0	13.366081780989873	0.0
19	1.3319596389590202E-4	0.0	0.0	14.014080145343435	0.0
20	1.3319596389590202E-4	0.0	0.0	14.670070267530754	0.0
21	1.3319596389590202E-4	0.0	0.0	15.550495588882665	0.0
22	1.3319596389590202E-4	0.0	0.0	16.498651057875644	0.0
23	1.3319596389590202E-4	0.0	0.0	17.376279263985744	0.0
24	1.3319596389590202E-4	0.0	0.0	18.081818284742337	0.0
25	1.3319596389590202E-4	0.0	0.0	18.70291106438893	0.0
26	1.3319596389590202E-4	0.0	0.0	19.266196795704698	0.0
27	1.3319596389590202E-4	0.0	0.0	19.810235710237507	0.0
28	1.3319596389590202E-4	0.0	0.0	20.390570524931952	0.0
29	1.3319596389590202E-4	0.0	0.0	21.02225238370827	0.0
30	1.3319596389590202E-4	0.0	0.0	21.70807840180827	0.0
31	1.3319596389590202E-4	0.0	0.0	22.335497989739913	0.0
32	1.3319596389590202E-4	0.0	0.0	22.93441364139784	0.0
33	1.3319596389590202E-4	0.0	0.0	23.516679797568774	0.0
34	1.3319596389590202E-4	0.0	0.0	24.102675440728795	0.0
35	1.3319596389590202E-4	0.0	0.0	24.733291731793944	0.0
36	1.3319596389590202E-4	0.0	0.0	25.358313792375462	0.0
37	1.3319596389590202E-4	0.0	0.0	25.947572736650933	0.0
38	1.3319596389590202E-4	0.0	0.0	26.530504872641348	0.0
39	1.3319596389590202E-4	0.0	0.0	27.140742181230426	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	25	3.8913786E-5	45.0	30
TTATGCG	25	3.8913786E-5	45.0	1
CCGTACC	20	7.033979E-4	45.0	31
CGTAAGG	25	3.8913786E-5	45.0	2
TCGTTAG	90	0.0	40.0	1
TTTGGGC	4125	0.0	39.381817	4
ACGAACG	40	3.4591721E-7	39.375	21
TCGTTTG	450	0.0	39.000004	1
ATACTCG	75	0.0	39.000004	44
CGTTTTT	3820	0.0	38.87434	1
CCGTTAT	35	6.249569E-6	38.571426	15
TTGGGAT	5190	0.0	38.540462	5
TTTGGGA	9630	0.0	38.457943	4
CGGGTAC	200	0.0	38.25	6
TGGGCGA	1960	0.0	38.22704	6
CGACCAA	330	0.0	38.18182	29
TTGGGAC	3575	0.0	37.510487	5
TTATCCG	30	1.1400885E-4	37.499996	1
CATACGA	265	0.0	37.35849	18
CGGGATC	615	0.0	37.317074	6
>>END_MODULE