FastQCFastQC Report
Sat 18 Jun 2016
SRR3549659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691014
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC22340.3232930157710263TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT20860.30187521526336664TruSeq Adapter, Index 20 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC20020.28971916632658673No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCG17960.25990790345781706No Hit
TCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC16290.2357405204525523TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC15250.22069017414987252TruSeq Adapter, Index 23 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC11980.17336841221740804No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCCTTTT8830.12778322870448355No Hit
CCTGTATCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC7640.11056215937737875TruSeq Adapter, Index 20 (95% over 24bp)
GCTGTATCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC7480.10824672148465878TruSeq Adapter, Index 20 (95% over 24bp)
CTGTCACTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT7390.1069442876700038TruSeq Adapter, Index 20 (96% over 25bp)
TCTGTATCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC7020.10158983754308885TruSeq Adapter, Index 20 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTT207.0313324E-445.019
AGTCAAC207.0313324E-445.033
GGTCGCC207.0313324E-445.032
CTATCCG207.0313324E-445.041
CAACCCG253.889185E-545.023
ATTCGTA207.0313324E-445.033
CGAATTC207.0313324E-445.026
ACCGTTA207.0313324E-445.020
GAACCGT207.0313324E-445.018
GCGCTTA253.889185E-545.027
TATAACG207.0313324E-445.043
CGATAGC253.889185E-545.012
ATCGCGA207.0313324E-445.021
AATCGTA253.889185E-545.012
TGCGTAG502.1827873E-1145.01
AACACGG207.0313324E-445.010
TCTCGAT302.1642409E-644.99999620
TAAATCG302.1642409E-644.99999628
TAGGGCG3250.042.9230775
TTGCGAG1000.042.751
Produced by FastQC (version 0.11.5)