##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549659_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	691014
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.955440844903286	33.0	31.0	34.0	30.0	34.0
2	32.1574888497194	33.0	31.0	34.0	30.0	34.0
3	32.24075778493634	34.0	31.0	34.0	30.0	34.0
4	35.689588054655914	37.0	35.0	37.0	35.0	37.0
5	29.327520137073922	37.0	35.0	37.0	0.0	37.0
6	23.433502070869764	35.0	10.0	37.0	0.0	37.0
7	30.281584743579725	35.0	19.0	37.0	17.0	37.0
8	34.176243896650426	35.0	32.0	37.0	32.0	37.0
9	36.8744251201857	38.0	37.0	39.0	34.0	39.0
10	36.953280251919644	39.0	37.0	39.0	34.0	39.0
11	37.12975279806198	39.0	37.0	39.0	34.0	39.0
12	37.11610184453571	39.0	37.0	39.0	34.0	39.0
13	37.04325961557942	39.0	37.0	39.0	33.0	39.0
14	38.1849716503573	40.0	37.0	41.0	33.0	41.0
15	38.062479197237685	40.0	37.0	41.0	33.0	41.0
16	38.301700110272726	40.0	37.0	41.0	34.0	41.0
17	38.28714903026567	40.0	37.0	41.0	34.0	41.0
18	38.355233034352416	40.0	37.0	41.0	34.0	41.0
19	38.363224189379665	40.0	37.0	41.0	34.0	41.0
20	38.28996084015664	40.0	37.0	41.0	34.0	41.0
21	38.18827838509784	40.0	37.0	41.0	34.0	41.0
22	38.24501240206421	40.0	37.0	41.0	34.0	41.0
23	38.17984874401966	40.0	37.0	41.0	34.0	41.0
24	38.159539459403135	40.0	37.0	41.0	34.0	41.0
25	37.9545653199501	40.0	37.0	41.0	34.0	41.0
26	37.83720735035759	40.0	36.0	41.0	33.0	41.0
27	36.93257155426663	40.0	36.0	41.0	32.0	41.0
28	37.32258970151111	40.0	36.0	41.0	32.0	41.0
29	37.63452260012098	40.0	36.0	41.0	33.0	41.0
30	37.66034986266559	40.0	36.0	41.0	33.0	41.0
31	37.71673511679937	40.0	36.0	41.0	33.0	41.0
32	37.714131696318745	40.0	36.0	41.0	33.0	41.0
33	36.4886181756086	40.0	35.0	41.0	31.0	41.0
34	37.00318517425117	40.0	35.0	41.0	31.0	41.0
35	37.31241335197261	40.0	35.0	41.0	31.0	41.0
36	37.42539948539393	40.0	36.0	41.0	33.0	41.0
37	37.50905915075527	40.0	36.0	41.0	33.0	41.0
38	37.478441826070096	40.0	36.0	41.0	33.0	41.0
39	37.488817882126845	40.0	36.0	41.0	33.0	41.0
40	37.46020485836756	40.0	36.0	41.0	33.0	41.0
41	37.361105563707824	39.0	35.0	41.0	33.0	41.0
42	37.41634322893603	40.0	35.0	41.0	33.0	41.0
43	37.29540211920453	39.0	35.0	41.0	33.0	41.0
44	37.32109190262426	39.0	35.0	41.0	33.0	41.0
45	37.252848712182384	39.0	35.0	41.0	33.0	41.0
46	37.177756167024114	39.0	35.0	41.0	33.0	41.0
47	37.1407004778485	39.0	35.0	41.0	33.0	41.0
48	37.12674996454486	39.0	35.0	41.0	33.0	41.0
49	37.15289125835366	39.0	35.0	41.0	33.0	41.0
50	37.064535595516155	39.0	35.0	41.0	33.0	41.0
51	36.41537653361582	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	9.0
12	6.0
13	6.0
14	10.0
15	9.0
16	25.0
17	33.0
18	94.0
19	163.0
20	328.0
21	618.0
22	1000.0
23	1433.0
24	2067.0
25	2863.0
26	3599.0
27	4452.0
28	5603.0
29	7382.0
30	10204.0
31	14634.0
32	20926.0
33	34300.0
34	47944.0
35	63586.0
36	79903.0
37	120345.0
38	141836.0
39	127527.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.61019024216586	4.643321264113317	52.90225668365619	16.84423181006463
2	19.264877412035066	6.118399916644235	52.22947726095275	22.38724541036795
3	20.41897848668768	6.256023756392779	50.46916560301239	22.85583215390716
4	18.74839004709022	5.0687829769006125	51.044551919353296	25.138275056655875
5	15.97044343529943	22.923848142005806	40.324653335533	20.781055087161764
6	16.656970770490904	31.22628485095816	38.29386958874929	13.822874789801654
7	82.08647002810363	2.243369888309065	10.792690162572685	4.877469921014625
8	82.87574491978454	3.9688052629903305	7.679439200942382	5.476010616282738
9	77.3189834069932	5.032459544958568	9.925413956880757	7.723143091167472
10	38.984014795648136	26.626378047333336	18.62017267378085	15.769434483237676
11	30.485923584760943	24.631773017623377	25.41453574023102	19.467767657384655
12	27.450239792536763	22.180882008179285	29.330375361425382	21.038502837858566
13	24.64783636800412	24.13178314766416	30.88302118336242	20.3373593009693
14	20.126220308126896	27.1771628360641	29.72457866266096	22.97203819314804
15	18.11960394434845	24.77605374131349	35.116365225596006	21.987977088742053
16	20.069347364886962	23.375213816217904	32.57401441938947	23.981424399505656
17	20.431279250492754	24.358551346282418	30.076814652090984	25.13335475113384
18	21.41866879686953	23.768404113375414	31.255951398958633	23.556975690796424
19	23.413707971184376	25.918143481897616	27.920128969890623	22.748019577027385
20	26.501199686258165	25.244785199720987	28.64297973702414	19.611035376996703
21	24.899061379364237	27.050826756042568	27.5765758725583	20.473535992034893
22	22.10490670232441	24.86664524886616	28.14877846179672	24.87966958701271
23	22.049770337504015	26.14954255630132	27.21507813155739	24.58560897463727
24	22.502004300925886	24.78705207130391	28.951656551097372	23.75928707667283
25	21.165417777353284	26.52117033808288	26.81349437203877	25.499917512525073
26	20.046482415696353	27.102779393760475	27.601032685300154	25.24970550524302
27	21.17149580182167	29.365830504157657	26.249540530293164	23.21313316372751
28	19.52319345194164	27.037657703027723	29.131826562124647	24.30732228290599
29	20.64719383398889	24.227005531002266	28.50911848385127	26.616682151157573
30	21.8737970576573	25.976608288688794	27.42491469058514	24.724679963068766
31	22.784487723837724	26.811323649014344	26.003380539323373	24.40080808782456
32	22.9500415331672	25.80512116975922	26.257644562917683	24.98719273415589
33	21.93573502128756	27.716515150199562	26.088907026485714	24.258842802027168
34	20.925191095983582	24.00269748514502	29.413441695826712	25.658669723044685
35	19.667329460763458	24.074910204424224	28.4445756525917	27.81318468222062
36	21.386976240712922	27.175136827907977	26.617839870103932	24.82004706127517
37	20.89957656429537	27.767888928444286	29.285513752253934	22.04702075500641
38	21.320986260770404	25.40194554668936	27.495101401708215	25.781966790832023
39	20.85703039301664	24.0351136156431	29.384064577562828	25.723791413777437
40	20.997693244999375	22.696935228519248	31.06854564451661	25.236825881964762
41	18.69079352950881	24.8683818272857	30.41805231153059	26.022772331674897
42	20.871936024451024	24.25001519506117	29.533699751379856	25.34434902910795
43	21.853392261227704	24.10399789295152	28.55904511341304	25.483564732407736
44	21.289438419482096	23.761457799697254	27.87801694321678	27.07108683760387
45	21.59131363474546	22.739770829534567	26.367917292558474	29.300998243161498
46	20.993641228687117	24.632641306833147	29.050496806142856	25.323220658336883
47	19.023348296850713	24.555942426636797	31.791975271123306	24.628734005389184
48	19.62304671106519	22.455695543071485	31.365066409653057	26.556191336210265
49	20.874106747475448	21.314618806565424	32.03741168775162	25.773862758207507
50	20.104657792750942	22.25367358693167	30.14497535505793	27.49669326525946
51	18.320902326146793	22.287392151244404	28.522576966602703	30.869128556006103
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	76.0
1	488.0
2	900.0
3	1288.5
4	1677.0
5	1291.0
6	905.0
7	924.5
8	944.0
9	1059.0
10	1174.0
11	1288.5
12	1403.0
13	1422.5
14	1442.0
15	1490.0
16	1538.0
17	1426.0
18	1314.0
19	1449.0
20	1584.0
21	1670.5
22	1757.0
23	2180.5
24	2604.0
25	3329.0
26	5103.0
27	6152.0
28	7171.5
29	8191.0
30	9416.5
31	10642.0
32	12379.0
33	14116.0
34	15975.5
35	17835.0
36	19719.5
37	21604.0
38	23267.0
39	24930.0
40	28209.5
41	31489.0
42	35978.0
43	40467.0
44	44492.5
45	48518.0
46	59742.5
47	70967.0
48	74713.0
49	78459.0
50	74349.0
51	70239.0
52	60974.5
53	51710.0
54	44931.0
55	38152.0
56	34941.5
57	31731.0
58	30711.0
59	29691.0
60	27316.0
61	24941.0
62	21024.5
63	17108.0
64	14231.0
65	11354.0
66	9460.5
67	7567.0
68	6336.0
69	5105.0
70	4304.0
71	3503.0
72	2915.0
73	2327.0
74	1849.0
75	1029.5
76	688.0
77	566.5
78	445.0
79	315.5
80	186.0
81	150.0
82	114.0
83	75.0
84	36.0
85	19.5
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	691014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.260009355011974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.12994287250116	26.875623462058723
2	10.808408389648951	8.054427954222195
3	4.0438026782572924	4.520163768650676
4	2.257688076025856	3.3648591533368957
5	1.544708779169933	2.877793178132041
6	1.2187525290584666	2.724643838049779
7	0.9979521553878313	2.6028594651923473
8	0.8088435394177313	2.4110014276356533
9	0.7040396192935674	2.360927052105965
>10	5.339252079389599	36.33426218013592
>50	0.1027070520084263	2.5979165638481785
>100	0.03826341132948492	2.664398965039639
>500	0.002819409255856784	0.7397106285164122
>1k	0.002819409255856784	1.8714123630756059
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	2234	0.3232930157710263	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT	2086	0.30187521526336664	TruSeq Adapter, Index 20 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC	2002	0.28971916632658673	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCG	1796	0.25990790345781706	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	1629	0.2357405204525523	TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	1525	0.22069017414987252	TruSeq Adapter, Index 23 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC	1198	0.17336841221740804	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCCTTTT	883	0.12778322870448355	No Hit
CCTGTATCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	764	0.11056215937737875	TruSeq Adapter, Index 20 (95% over 24bp)
GCTGTATCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	748	0.10824672148465878	TruSeq Adapter, Index 20 (95% over 24bp)
CTGTCACTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT	739	0.1069442876700038	TruSeq Adapter, Index 20 (96% over 25bp)
TCTGTATCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	702	0.10158983754308885	TruSeq Adapter, Index 20 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4471486829499837E-4	0.0	0.0	0.32387187524420635	0.0
2	1.4471486829499837E-4	0.0	0.0	1.1766765941066317	0.0
3	1.4471486829499837E-4	0.0	0.0	1.6187805167478517	0.0
4	1.4471486829499837E-4	0.0	0.0	2.109363920267896	0.0
5	1.4471486829499837E-4	0.0	0.0	3.0356837922241806	0.0
6	1.4471486829499837E-4	0.0	0.0	3.6210554344774493	0.0
7	1.4471486829499837E-4	0.0	0.0	4.3931092568312655	0.0
8	1.4471486829499837E-4	0.0	0.0	5.627671798255896	0.0
9	1.4471486829499837E-4	0.0	0.0	6.127951097951706	0.0
10	1.4471486829499837E-4	0.0	0.0	7.4377653708897356	0.0
11	1.4471486829499837E-4	0.0	0.0	9.29489127571945	0.0
12	1.4471486829499837E-4	0.0	0.0	11.177776427105673	0.0
13	1.4471486829499837E-4	0.0	0.0	11.733770951095057	0.0
14	1.4471486829499837E-4	0.0	0.0	11.93072788684455	0.0
15	1.4471486829499837E-4	0.0	0.0	12.277898855884251	0.0
16	1.4471486829499837E-4	0.0	0.0	12.988883003817579	0.0
17	1.4471486829499837E-4	0.0	0.0	13.945014138642632	0.0
18	1.4471486829499837E-4	0.0	0.0	14.9325483998877	0.0
19	1.4471486829499837E-4	0.0	0.0	15.783327110593996	0.0
20	1.4471486829499837E-4	0.0	0.0	16.4834576434052	0.0
21	1.4471486829499837E-4	0.0	0.0	17.426275010347112	0.0
22	1.4471486829499837E-4	0.0	0.0	18.428280758421682	0.0
23	1.4471486829499837E-4	0.0	0.0	19.37847858364664	0.0
24	1.4471486829499837E-4	0.0	0.0	20.123181295892703	0.0
25	1.4471486829499837E-4	0.0	0.0	20.750375535083226	0.0
26	1.4471486829499837E-4	0.0	0.0	21.31476352143372	0.0
27	1.4471486829499837E-4	0.0	0.0	21.872784053579235	0.0
28	1.4471486829499837E-4	0.0	0.0	22.46365486082771	0.0
29	1.4471486829499837E-4	0.0	0.0	23.09157267435971	0.0
30	1.4471486829499837E-4	0.0	0.0	23.78880890980501	0.0
31	1.4471486829499837E-4	0.0	0.0	24.438289238712965	0.0
32	1.4471486829499837E-4	0.0	0.0	25.031475483854162	0.0
33	1.4471486829499837E-4	0.0	0.0	25.6090325232195	0.0
34	1.4471486829499837E-4	0.0	0.0	26.19006271942392	0.0
35	1.4471486829499837E-4	0.0	0.0	26.818993537033982	0.0
36	1.4471486829499837E-4	0.0	0.0	27.446477205961095	0.0
37	1.4471486829499837E-4	0.0	0.0	28.05051706622442	0.0
38	1.4471486829499837E-4	0.0	0.0	28.721559910508326	0.0
39	1.4471486829499837E-4	0.0	0.0	29.355555748508714	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTT	20	7.0313324E-4	45.0	19
AGTCAAC	20	7.0313324E-4	45.0	33
GGTCGCC	20	7.0313324E-4	45.0	32
CTATCCG	20	7.0313324E-4	45.0	41
CAACCCG	25	3.889185E-5	45.0	23
ATTCGTA	20	7.0313324E-4	45.0	33
CGAATTC	20	7.0313324E-4	45.0	26
ACCGTTA	20	7.0313324E-4	45.0	20
GAACCGT	20	7.0313324E-4	45.0	18
GCGCTTA	25	3.889185E-5	45.0	27
TATAACG	20	7.0313324E-4	45.0	43
CGATAGC	25	3.889185E-5	45.0	12
ATCGCGA	20	7.0313324E-4	45.0	21
AATCGTA	25	3.889185E-5	45.0	12
TGCGTAG	50	2.1827873E-11	45.0	1
AACACGG	20	7.0313324E-4	45.0	10
TCTCGAT	30	2.1642409E-6	44.999996	20
TAAATCG	30	2.1642409E-6	44.999996	28
TAGGGCG	325	0.0	42.923077	5
TTGCGAG	100	0.0	42.75	1
>>END_MODULE