FastQCFastQC Report
Sat 18 Jun 2016
SRR3549656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513397
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC35760.23628961865260734TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT31240.2064230337446156Illumina PCR Primer Index 4 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC23580.15580842303770923TruSeq Adapter, Index 13 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC20120.1329459487497332TruSeq Adapter, Index 13 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAT207.033996E-445.033
TCGTTAG2200.042.9545441
TAGGGCG5900.041.56785
TACGGGT1300.041.538464
TTAGGGA42600.041.302824
CTTTACG2650.040.754721
TAGGGAA17950.040.4874655
TAGGGAT27450.040.327875
GCGCGAC3350.040.2985089
AGGGCGA7900.040.158236
TTCGTAG2150.039.767441
TATGGGA35400.039.661024
ATGGGCG4850.039.432995
TTACGGG17450.039.326653
ACACGAC1950.039.2307726
CGTTAGG4650.039.193552
CTAGGGA22100.039.0950244
TTTACGG10550.039.0284352
TAGGGAG17550.038.9743585
TCAAGCG2150.038.72093217
Produced by FastQC (version 0.11.5)