##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549656_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1513397
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.064517109522484	33.0	31.0	34.0	30.0	34.0
2	32.28245595835065	34.0	31.0	34.0	30.0	34.0
3	32.36052932574863	34.0	31.0	34.0	30.0	34.0
4	35.757957099161686	37.0	35.0	37.0	35.0	37.0
5	29.485852026930143	37.0	35.0	37.0	0.0	37.0
6	23.611900909014622	35.0	10.0	37.0	0.0	37.0
7	30.423832609685363	35.0	19.0	37.0	17.0	37.0
8	34.41087566580349	35.0	32.0	37.0	32.0	37.0
9	37.026799313068544	38.0	37.0	39.0	34.0	39.0
10	37.033858927961404	39.0	37.0	39.0	34.0	39.0
11	37.16083618508561	39.0	37.0	39.0	34.0	39.0
12	37.183470695395854	39.0	37.0	39.0	34.0	39.0
13	37.190666427910195	39.0	37.0	39.0	34.0	39.0
14	38.33302431549686	40.0	38.0	41.0	34.0	41.0
15	38.187284631858	40.0	38.0	41.0	34.0	41.0
16	38.3838682117118	40.0	38.0	41.0	34.0	41.0
17	38.35658323625592	40.0	37.0	41.0	34.0	41.0
18	38.42323858181297	40.0	38.0	41.0	34.0	41.0
19	38.45904676697522	40.0	38.0	41.0	34.0	41.0
20	38.43469030267669	40.0	38.0	41.0	34.0	41.0
21	38.31572284073511	40.0	37.0	41.0	34.0	41.0
22	38.38984681481462	40.0	37.0	41.0	34.0	41.0
23	38.33687261174695	40.0	37.0	41.0	34.0	41.0
24	38.33533104664539	40.0	37.0	41.0	34.0	41.0
25	38.108643006428586	40.0	37.0	41.0	34.0	41.0
26	38.003345453968784	40.0	37.0	41.0	34.0	41.0
27	37.17052630605188	40.0	36.0	41.0	33.0	41.0
28	37.54725627181764	40.0	36.0	41.0	33.0	41.0
29	37.83340590737262	40.0	36.0	41.0	33.0	41.0
30	37.84768702462077	40.0	36.0	41.0	33.0	41.0
31	37.888297650913806	40.0	37.0	41.0	34.0	41.0
32	37.88824743276219	40.0	37.0	41.0	34.0	41.0
33	36.68978992293496	40.0	36.0	41.0	32.0	41.0
34	37.14771669297613	40.0	36.0	41.0	32.0	41.0
35	37.454346083677976	40.0	36.0	41.0	32.0	41.0
36	37.625591302216144	40.0	36.0	41.0	33.0	41.0
37	37.771634937825304	40.0	36.0	41.0	33.0	41.0
38	37.71300722811001	40.0	36.0	41.0	33.0	41.0
39	37.71361381052031	40.0	36.0	41.0	33.0	41.0
40	37.66429826410387	40.0	36.0	41.0	33.0	41.0
41	37.609160715925825	40.0	36.0	41.0	33.0	41.0
42	37.66377295580737	40.0	36.0	41.0	33.0	41.0
43	37.583564656200586	40.0	36.0	41.0	33.0	41.0
44	37.606813017337814	40.0	36.0	41.0	33.0	41.0
45	37.53163181901378	40.0	36.0	41.0	33.0	41.0
46	37.472827024237525	40.0	35.0	41.0	33.0	41.0
47	37.4455889631075	39.0	35.0	41.0	33.0	41.0
48	37.40389666425928	39.0	35.0	41.0	33.0	41.0
49	37.421859564939005	39.0	35.0	41.0	33.0	41.0
50	37.3440359667688	39.0	35.0	41.0	33.0	41.0
51	36.65632150717888	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	9.0
10	14.0
11	7.0
12	18.0
13	13.0
14	27.0
15	35.0
16	45.0
17	93.0
18	167.0
19	267.0
20	489.0
21	832.0
22	1338.0
23	2158.0
24	3292.0
25	4912.0
26	6928.0
27	8822.0
28	11397.0
29	15050.0
30	20885.0
31	29604.0
32	43368.0
33	73197.0
34	100493.0
35	129300.0
36	165249.0
37	262303.0
38	326003.0
39	306879.0
40	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.27353430725712	4.256384808480524	55.928616219009285	17.54146466525307
2	19.342512242326368	4.056437273233659	55.656248823012064	20.9448016614279
3	20.75456737392766	4.01599844588036	53.81628217843699	21.41315200175499
4	19.048669978862122	4.361446467780761	51.939114455757476	24.65076909759964
5	15.221055678054075	22.300427448977366	41.78269152112763	20.695825351840924
6	16.697205029480035	29.229475147631454	39.949134298535014	14.124185524353491
7	85.71630576775294	1.6193371600445885	8.378105678813954	4.2862513933885165
8	87.72615513312105	1.9026071810635279	6.010385906672208	4.360851779143212
9	81.72191434237018	5.434991611586385	8.166660829907817	4.676433216135621
10	38.60203238145708	35.41853195162935	13.792151035055575	12.187284631857999
11	27.304335874856367	23.57213606211721	30.035146098479114	19.088381964547306
12	26.429152429930813	21.214922455905487	31.98499798797011	20.37092712619359
13	24.71050226741562	21.22853421805382	33.07704455605502	20.983918958475538
14	20.13985755224835	24.31787561360304	32.52484311783359	23.01742371631502
15	18.6526734227701	25.491790984123796	35.3194171787046	20.536118414401507
16	23.1843329939203	23.690016565382383	32.04836536612667	21.07728507457065
17	22.08455547354726	24.14012978749132	30.642719656507843	23.13259508245358
18	21.525482077736378	23.78754550194034	31.88006848170044	22.806903938622845
19	23.546432297672055	25.470976881809598	29.775201087355136	21.20738973316321
20	25.40562720819455	26.985582765130367	29.141395152758992	18.4673948739161
21	25.294552586003537	26.344111954761374	28.840218396098315	19.52111706313677
22	22.762830902928975	24.152750401910406	30.180976967709068	22.903441727451554
23	21.949759382369596	25.18955700321859	30.365925133986654	22.494758480425162
24	23.382364310223952	23.232502773561727	30.474819231173317	22.910313685041004
25	22.688494823235413	24.209113669446946	27.79336816446709	25.309023342850555
26	20.172301121252385	25.860960475010852	28.879005310569532	25.087733093167223
27	19.579198320070677	27.36816578861991	29.87206925876026	23.18056663254916
28	18.11216752775379	26.33195387594927	30.64430549287464	24.911573103422302
29	20.013915714118635	25.755832739195334	29.62177141886762	24.60848012781841
30	22.584886847271406	24.567050152735863	30.21434560792707	22.633717392065662
31	22.57669335937629	25.76918019528253	28.385479817919556	23.26864662742162
32	22.085612697791788	26.836514146651542	27.855215782772135	23.222657372784536
33	22.239570978401566	27.666435178608122	26.9387345157946	23.155259327195708
34	21.160805789888578	24.753055543257982	29.671857417452262	24.41428124940118
35	19.60833806331055	25.26197686396894	29.899689242148625	25.229995830571884
36	20.060235351332135	27.339422504471727	27.971378296639944	24.628963847556193
37	20.455108606664346	27.32759480823604	29.228285770356354	22.989010814743256
38	20.063539177096292	27.889773800265232	27.78504252354141	24.261644499097066
39	21.243203204446683	26.871600776266902	27.496155998723403	24.38904002056301
40	22.7054104111479	24.675349561284975	29.174235180854723	23.445004846712397
41	20.110321349916777	24.515510470814995	29.380988597175755	25.993179582092473
42	19.683863520279214	24.344174066685742	29.120118514837813	26.851843898197238
43	20.02647025202244	23.478307410415113	28.750882947435468	27.74433939012698
44	19.917510078320493	24.019870529675956	28.683220595785507	27.379398796218048
45	21.18842577327694	23.710566361635447	27.554964097325417	27.54604376776219
46	21.182941422508435	24.187638801979915	29.404445760101282	25.224974015410368
47	18.177385048338273	24.69523859238521	31.36090530112059	25.766471058155922
48	18.630736019696087	23.733428835923423	31.35383511398529	26.282000030395196
49	20.841920527132007	23.08356630811347	31.090520200581867	24.983992964172653
50	20.15564983940103	23.19417839469749	29.401604469944104	27.248567295957372
51	18.48774644062331	22.389036055972095	29.27863607500213	29.84458142840246
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	261.0
1	844.0
2	1427.0
3	2993.0
4	4559.0
5	3241.5
6	1924.0
7	1795.0
8	1666.0
9	1750.5
10	1835.0
11	1887.5
12	1940.0
13	2015.5
14	2091.0
15	2215.0
16	2339.0
17	2385.5
18	2432.0
19	2883.5
20	3335.0
21	4082.5
22	4830.0
23	6019.0
24	7208.0
25	9538.0
26	14062.5
27	16257.0
28	20574.0
29	24891.0
30	27706.0
31	30521.0
32	34287.5
33	38054.0
34	41678.5
35	45303.0
36	50047.0
37	54791.0
38	60043.0
39	65295.0
40	71868.0
41	78441.0
42	85793.5
43	93146.0
44	100936.5
45	108727.0
46	121895.0
47	135063.0
48	148235.0
49	161407.0
50	153288.5
51	145170.0
52	126552.0
53	107934.0
54	94777.5
55	81621.0
56	74096.5
57	66572.0
58	64741.5
59	62911.0
60	57345.0
61	51779.0
62	44478.5
63	37178.0
64	30693.5
65	24209.0
66	19169.0
67	14129.0
68	11390.0
69	8651.0
70	6976.5
71	5302.0
72	4335.0
73	3368.0
74	2834.5
75	1893.5
76	1486.0
77	1070.5
78	655.0
79	479.5
80	304.0
81	220.5
82	137.0
83	105.5
84	74.0
85	39.5
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1513397.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.29892165439382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.40470873733017	25.55615006556764
2	11.620625134114222	8.436323226365838
3	4.331435999163193	4.716793679539374
4	2.246373288042262	3.2616371195667653
5	1.5172725267544553	2.753767828851207
6	1.1550683224961171	2.515664072625519
7	0.973652887908065	2.47397849277244
8	0.7991276725835436	2.3205978223174504
9	0.691706051308974	2.259736538790111
>10	6.130447549237986	39.78687230365275
>50	0.09231246919897244	2.2897366751503907
>100	0.03376892761536508	2.0098976992565203
>500	0.0020590789686429955	0.5242428159871567
>1k	0.001441355278050097	1.0946016595568733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	3576	0.23628961865260734	TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	3124	0.2064230337446156	Illumina PCR Primer Index 4 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	2358	0.15580842303770923	TruSeq Adapter, Index 13 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	2012	0.1329459487497332	TruSeq Adapter, Index 13 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9822954584950282E-4	0.0	0.0	0.22175278529031048	0.0
2	1.9822954584950282E-4	0.0	0.0	0.7964863152233023	0.0
3	1.9822954584950282E-4	0.0	0.0	1.0953503938490694	0.0
4	1.9822954584950282E-4	0.0	0.0	1.4514367347100596	0.0
5	1.9822954584950282E-4	0.0	0.0	2.0527991003021677	0.0
6	1.9822954584950282E-4	0.0	0.0	2.5613900384367088	0.0
7	1.9822954584950282E-4	0.0	0.0	3.2043145321419297	0.0
8	1.9822954584950282E-4	0.0	0.0	4.230482814489522	0.0
9	1.9822954584950282E-4	0.0	0.0	4.708480326047957	0.0
10	1.9822954584950282E-4	0.0	0.0	5.579302720964823	0.0
11	1.9822954584950282E-4	0.0	0.0	7.33046252899933	0.0
12	1.9822954584950282E-4	0.0	0.0	9.060543928658507	0.0
13	1.9822954584950282E-4	0.0	0.0	9.557307170557362	0.0
14	1.9822954584950282E-4	0.0	0.0	9.731683094389641	0.0
15	1.9822954584950282E-4	0.0	0.0	9.99156202899834	0.0
16	1.9822954584950282E-4	0.0	0.0	10.600589270363296	0.0
17	1.9822954584950282E-4	0.0	0.0	11.49936203124494	0.0
18	1.9822954584950282E-4	0.0	0.0	12.464607766501453	0.0
19	1.9822954584950282E-4	0.0	0.0	13.21444406193484	0.0
20	1.9822954584950282E-4	0.0	0.0	14.021899078695148	0.0
21	1.9822954584950282E-4	0.0	0.0	15.06974045805562	0.0
22	1.9822954584950282E-4	0.0	0.0	16.25356730586885	0.0
23	2.643060611326704E-4	0.0	0.0	17.30015323143894	0.0
24	2.643060611326704E-4	0.0	0.0	18.117585802007007	0.0
25	2.643060611326704E-4	0.0	0.0	18.844956082244117	0.0
26	2.643060611326704E-4	0.0	0.0	19.46435733650853	0.0
27	3.30382576415838E-4	0.0	0.0	20.09829542413524	0.0
28	3.30382576415838E-4	0.0	0.0	20.76243047924636	0.0
29	3.30382576415838E-4	0.0	0.0	21.470176034444364	0.0
30	3.30382576415838E-4	0.0	0.0	22.253248817065185	0.0
31	3.30382576415838E-4	0.0	0.0	22.95465102679601	0.0
32	3.30382576415838E-4	0.0	0.0	23.64739721302474	0.0
33	3.30382576415838E-4	0.0	0.0	24.343182918956494	0.0
34	3.30382576415838E-4	0.0	0.0	25.060773874931694	0.0
35	3.30382576415838E-4	0.0	0.0	25.782924110461433	0.0
36	3.30382576415838E-4	0.0	0.0	26.495625404305677	0.0
37	3.30382576415838E-4	0.0	0.0	27.213877125433708	0.0
38	3.30382576415838E-4	0.0	0.0	27.967215476177103	0.0
39	3.30382576415838E-4	0.0	0.0	28.831231990019802	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAT	20	7.033996E-4	45.0	33
TCGTTAG	220	0.0	42.954544	1
TAGGGCG	590	0.0	41.5678	5
TACGGGT	130	0.0	41.53846	4
TTAGGGA	4260	0.0	41.30282	4
CTTTACG	265	0.0	40.75472	1
TAGGGAA	1795	0.0	40.487465	5
TAGGGAT	2745	0.0	40.32787	5
GCGCGAC	335	0.0	40.298508	9
AGGGCGA	790	0.0	40.15823	6
TTCGTAG	215	0.0	39.76744	1
TATGGGA	3540	0.0	39.66102	4
ATGGGCG	485	0.0	39.43299	5
TTACGGG	1745	0.0	39.32665	3
ACACGAC	195	0.0	39.23077	26
CGTTAGG	465	0.0	39.19355	2
CTAGGGA	2210	0.0	39.095024	4
TTTACGG	1055	0.0	39.028435	2
TAGGGAG	1755	0.0	38.974358	5
TCAAGCG	215	0.0	38.720932	17
>>END_MODULE