FastQCFastQC Report
Sat 18 Jun 2016
SRR3549655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504917
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGC42470.8411283438664177No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG36210.7171475707888624No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC25020.49552698760390324No Hit
CCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC18700.3703579004073937No Hit
CTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT14160.28044213207319224No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC14110.2794518703073971No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAAGCAGT13300.2634096297015153No Hit
TCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC10510.20815302317014478No Hit
GAATCAGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC9540.1889419449137185No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCT8110.1606204584119766No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTC7800.15448083546404656No Hit
GCTGTATCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC6720.1330911813228709No Hit
CCTGTATCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC6280.1243768777838734No Hit
CTGTCACTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT6190.12259440660544209No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTA6160.12200024954596499No Hit
GAATATGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC5380.10655216599956031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATC351.2101191E-745.00000427
GGGTACG351.2101191E-745.0000047
CTACGAA700.045.00000411
TACGAAT700.045.00000412
TTGCGTA351.2101191E-745.00000436
GCGACCG207.029526E-445.033
CAACGTC253.8876875E-545.019
ACCGCGA253.8876875E-545.011
GACAACG207.029526E-445.026
ACCGAAC207.029526E-445.011
TTACTCG207.029526E-445.039
CGCGACT253.8876875E-545.013
TCGGCAC207.029526E-445.027
TACGCTT207.029526E-445.039
GCCGATT453.8380676E-1045.09
CGTACTA253.8876875E-545.011
ACGTATA207.029526E-445.014
CAATCCG207.029526E-445.013
TTCGATT207.029526E-445.037
CCGCACG207.029526E-445.039