##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549655_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504917
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.937847210531633	33.0	31.0	34.0	30.0	34.0
2	32.115684360003726	33.0	31.0	34.0	30.0	34.0
3	32.20940273351858	34.0	31.0	34.0	30.0	34.0
4	35.68956680008794	37.0	35.0	37.0	35.0	37.0
5	29.253297076549217	37.0	35.0	37.0	0.0	37.0
6	23.503460964871454	35.0	10.0	37.0	0.0	37.0
7	30.257491825389124	35.0	19.0	37.0	17.0	37.0
8	33.92656416797216	35.0	32.0	37.0	32.0	37.0
9	36.75040848297839	37.0	35.0	39.0	34.0	39.0
10	36.97754680472236	39.0	37.0	39.0	34.0	39.0
11	37.14692909923809	39.0	37.0	39.0	34.0	39.0
12	37.093647074667714	39.0	37.0	39.0	34.0	39.0
13	36.938334419320405	39.0	37.0	39.0	33.0	39.0
14	38.12319450523552	40.0	37.0	41.0	33.0	41.0
15	37.98514409298954	40.0	37.0	41.0	33.0	41.0
16	38.26432463157311	40.0	37.0	41.0	34.0	41.0
17	38.25593909494036	40.0	37.0	41.0	34.0	41.0
18	38.34591427898051	40.0	37.0	41.0	34.0	41.0
19	38.33691676057649	40.0	37.0	41.0	34.0	41.0
20	38.19618075842168	40.0	37.0	41.0	34.0	41.0
21	38.12365200617131	40.0	37.0	41.0	34.0	41.0
22	38.179175983379444	40.0	37.0	41.0	34.0	41.0
23	38.113846434166405	40.0	37.0	41.0	34.0	41.0
24	38.11359490767789	40.0	37.0	41.0	34.0	41.0
25	37.88803110214154	40.0	36.0	41.0	33.0	41.0
26	37.732769940406044	40.0	36.0	41.0	33.0	41.0
27	36.785461768964005	39.0	35.0	41.0	32.0	41.0
28	37.13814349685196	39.0	36.0	41.0	32.0	41.0
29	37.447348772174436	39.0	36.0	41.0	32.0	41.0
30	37.52930481643518	39.0	36.0	41.0	33.0	41.0
31	37.508966820289274	39.0	36.0	41.0	33.0	41.0
32	37.43108669345655	39.0	35.0	41.0	33.0	41.0
33	36.22628273557832	39.0	35.0	41.0	31.0	41.0
34	36.657862579394234	39.0	35.0	41.0	31.0	41.0
35	37.01611750050008	39.0	35.0	41.0	31.0	41.0
36	37.15813094033277	39.0	35.0	41.0	32.0	41.0
37	37.17258678158984	39.0	35.0	41.0	33.0	41.0
38	37.09128827114159	39.0	35.0	41.0	32.0	41.0
39	37.18197248260605	39.0	35.0	41.0	33.0	41.0
40	37.11489611163815	39.0	35.0	41.0	33.0	41.0
41	37.038820241742705	39.0	35.0	41.0	32.0	41.0
42	37.04272979519406	39.0	35.0	41.0	32.0	41.0
43	36.93921575229196	39.0	35.0	40.0	32.0	41.0
44	36.9790539831299	39.0	35.0	40.0	32.0	41.0
45	36.92617004378938	39.0	35.0	40.0	32.0	41.0
46	36.895499656379165	39.0	35.0	40.0	32.0	41.0
47	36.79227080886562	39.0	35.0	40.0	32.0	41.0
48	36.79507324966281	39.0	35.0	40.0	32.0	41.0
49	36.80643551316355	39.0	35.0	40.0	32.0	41.0
50	36.71261811347212	39.0	35.0	40.0	32.0	41.0
51	35.987956436404396	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	7.0
11	2.0
12	5.0
13	4.0
14	9.0
15	17.0
16	20.0
17	33.0
18	66.0
19	115.0
20	290.0
21	600.0
22	952.0
23	1359.0
24	1819.0
25	2330.0
26	2796.0
27	3319.0
28	4242.0
29	5611.0
30	7957.0
31	11368.0
32	16600.0
33	26953.0
34	37223.0
35	50140.0
36	60631.0
37	88479.0
38	99587.0
39	82308.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.01569366846432	4.481924751988941	50.49463575201468	17.00774582753205
2	18.874983413115423	9.377976974433421	50.66634714220357	21.080692470247588
3	20.185297781615592	9.239340327222099	49.27661378008663	21.298748111075682
4	18.604047793993864	4.875652830069101	51.5746152337909	24.945684142146135
5	18.04712457691066	23.299671035041403	37.51784946832846	21.13535491971948
6	16.002234030543633	32.45800794982146	37.11283240611824	14.426925613516678
7	78.71471944893914	2.889385780237148	13.00748439842588	5.388410372397839
8	77.99182835990865	6.990059752394948	8.59111497533258	6.426996912363814
9	73.15697431459031	5.543485364921363	10.605307406959955	10.694232913528362
10	42.21882012291129	22.440321874684354	19.235834800571183	16.10502320183317
11	31.309304301499058	23.89521446099062	23.804110378537462	20.99137085897286
12	28.233749309292417	19.700861725788595	29.54426173014575	22.52112723477324
13	24.45431625395857	23.951065224581466	31.310888720324332	20.28372980113563
14	19.76106964114894	28.328616386455597	27.608101925663032	24.302212046732432
15	16.187412980747332	24.215861220755095	37.9537626976315	21.642963100866083
16	18.153478690557062	22.71403022675014	32.9567037750759	26.175787307616893
17	18.838145675427842	23.196881863751866	30.05008744011392	27.914885020706375
18	19.918521261910374	22.84296230865667	31.823250157946752	25.415266271486203
19	22.96199177290525	24.245767126082107	27.73901453110115	25.05322656991149
20	26.74677224177439	24.41767656862415	29.188361651518964	19.647189538082497
21	24.590180168225668	25.945056316186623	27.653852019242763	21.810911496344946
22	22.072934759574345	22.985163898224855	28.019060558468023	26.92284078373277
23	22.771267356813098	25.94683878736505	27.176545848129496	24.105348007692353
24	23.22005398907147	22.597575443092627	28.602126686168223	25.58024388166768
25	20.498616604313185	26.932743401390724	26.96839282495935	25.600247169336743
26	20.768958066375266	26.760833958848686	27.766345755837097	24.703862218938955
27	22.98674831705013	29.403446507049676	25.98664731035002	21.62315786555018
28	18.678119374075344	26.92957456374018	31.201959926086865	23.190346136097617
29	21.48293679951358	25.144726757070963	28.50765571371136	24.864680729704087
30	23.641707448947056	26.86639586308245	26.12171901520448	23.37017767276602
31	22.973082704682156	28.389814563581737	22.636591756664956	26.00051097507115
32	25.077190904643732	30.76743306325594	22.185032391462357	21.970343640637964
33	25.45131179976115	28.571230519075414	22.618568992527486	23.358888688635954
34	21.453625051246046	26.837876324227544	25.481019652735004	26.227478971791403
35	23.276102805015476	27.08049045684736	24.27824771200019	25.36515902613697
36	25.93297512264392	28.033320327895478	22.90079359577911	23.132910953681495
37	23.179057151967548	29.091315998471035	26.50950552268987	21.220121326871542
38	22.7427478179582	29.505641521279735	23.891253413927437	23.860357246834628
39	24.170309179528516	26.652499321670692	25.784831962480965	23.39235953631983
40	24.342218622070558	23.75954859907668	27.55284531913166	24.345387459721103
41	20.38077545418356	25.780078706005146	25.22988926892935	28.609256570881946
42	22.43497446114906	26.48910613031449	26.694882525246722	24.381036883289728
43	25.37367527732281	24.896369106209534	25.63530243584589	24.094653180621766
44	22.829692800995016	24.69455375834048	27.05216897034562	25.423584470318882
45	22.068775660158003	24.576712608210855	25.02668755458818	28.32782417704296
46	24.03008811349192	26.816684722439533	25.772354664231944	23.38087249983661
47	20.275213549949793	25.38694478498446	30.61037754720083	23.727464117864915
48	21.71366779094386	24.361627752680143	27.436984692533624	26.487719763842378
49	22.1355193031726	23.182225989618097	30.59156257365072	24.090692133558587
50	23.321852898595214	23.55040531414074	26.940863547870244	26.186878239393803
51	20.75113335459095	24.306371146148773	24.224377471940933	30.718118027319342
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97.0
1	341.0
2	585.0
3	1011.5
4	1438.0
5	1051.0
6	664.0
7	690.0
8	716.0
9	756.5
10	797.0
11	824.0
12	851.0
13	836.5
14	822.0
15	772.0
16	722.0
17	670.0
18	618.0
19	671.0
20	724.0
21	920.0
22	1116.0
23	1450.0
24	1784.0
25	2019.5
26	2825.5
27	3396.0
28	4060.5
29	4725.0
30	5420.5
31	6116.0
32	7365.0
33	8614.0
34	9987.5
35	11361.0
36	12015.0
37	12669.0
38	14460.0
39	16251.0
40	19439.0
41	22627.0
42	26310.0
43	29993.0
44	34453.5
45	38914.0
46	49658.0
47	60402.0
48	58610.5
49	56819.0
50	53172.0
51	49525.0
52	42996.5
53	36468.0
54	31429.5
55	26391.0
56	24636.5
57	22882.0
58	22706.5
59	22531.0
60	20932.0
61	19333.0
62	16721.0
63	14109.0
64	11994.5
65	9880.0
66	8415.5
67	6951.0
68	5721.0
69	4491.0
70	3636.0
71	2781.0
72	2425.5
73	2070.0
74	1679.0
75	1007.0
76	726.0
77	513.5
78	301.0
79	188.0
80	75.0
81	52.0
82	29.0
83	18.5
84	8.0
85	4.5
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	504917.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.004982402881105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.68212742727756	27.62282973877646
2	10.814646828100289	8.220209247906508
3	4.007024598180609	4.568606980252974
4	2.1799268454107996	3.313923255976223
5	1.5636372581149773	2.9713003239574483
6	1.2123878560836663	2.7646067481555896
7	0.9524299590282315	2.53379586829913
8	0.8188281851677334	2.4895640614626218
9	0.6540202924742569	2.2370426735950155
>10	4.997688622465047	34.09485121157086
>50	0.07551041098291178	1.9463566036906972
>100	0.03320315943929266	2.54229220293835
>500	0.004284278637328084	1.1434310780648187
>1k	0.004284278637328084	3.551190005353321
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGC	4247	0.8411283438664177	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG	3621	0.7171475707888624	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC	2502	0.49552698760390324	No Hit
CCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	1870	0.3703579004073937	No Hit
CTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT	1416	0.28044213207319224	No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	1411	0.2794518703073971	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAAGCAGT	1330	0.2634096297015153	No Hit
TCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	1051	0.20815302317014478	No Hit
GAATCAGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC	954	0.1889419449137185	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCT	811	0.1606204584119766	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTC	780	0.15448083546404656	No Hit
GCTGTATCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	672	0.1330911813228709	No Hit
CCTGTATCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	628	0.1243768777838734	No Hit
CTGTCACTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT	619	0.12259440660544209	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTA	616	0.12200024954596499	No Hit
GAATATGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC	538	0.10655216599956031	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31371492740390994	0.0
2	0.0	0.0	0.0	1.2798143061136782	0.0
3	0.0	0.0	0.0	1.733948351907343	0.0
4	0.0	0.0	0.0	2.261757873076169	0.0
5	0.0	0.0	0.0	3.5225591532865796	0.0
6	0.0	0.0	0.0	4.109190223343639	0.0
7	0.0	0.0	0.0	5.039640178484781	0.0
8	0.0	0.0	0.0	6.328168788137456	0.0
9	0.0	0.0	0.0	6.820130833384497	0.0
10	0.0	0.0	0.0	8.705193130752184	0.0
11	0.0	0.0	0.0	10.514599429213119	0.0
12	0.0	0.0	0.0	13.157211977414109	0.0
13	0.0	0.0	0.0	13.738099529229556	0.0
14	0.0	0.0	0.0	13.965265578302969	0.0
15	0.0	0.0	0.0	14.451880210014716	0.0
16	0.0	0.0	0.0	15.223690230275471	0.0
17	0.0	0.0	0.0	16.289013837917913	0.0
18	0.0	0.0	0.0	17.45653245979042	0.0
19	0.0	0.0	0.0	18.480265073269468	0.0
20	0.0	0.0	0.0	19.274653061790353	0.0
21	0.0	0.0	0.0	20.280560963485087	0.0
22	3.961047063180681E-4	0.0	0.0	21.32152413168897	0.0
23	3.961047063180681E-4	0.0	0.0	22.27118516508654	0.0
24	3.961047063180681E-4	0.0	0.0	23.042599080640976	0.0
25	3.961047063180681E-4	0.0	0.0	23.72924658904335	0.0
26	3.961047063180681E-4	0.0	0.0	24.32716664323047	0.0
27	3.961047063180681E-4	0.0	0.0	24.924690592711276	0.0
28	3.961047063180681E-4	0.0	0.0	25.533701578675306	0.0
29	3.961047063180681E-4	0.0	0.0	26.157170386419946	0.0
30	3.961047063180681E-4	0.0	0.0	26.85966208307504	0.0
31	3.961047063180681E-4	0.0	0.0	27.53442645028787	0.0
32	3.961047063180681E-4	0.0	0.0	28.196119362192203	0.0
33	3.961047063180681E-4	0.0	0.0	28.77680886165449	0.0
34	3.961047063180681E-4	0.0	0.0	29.387206214090632	0.0
35	3.961047063180681E-4	0.0	0.0	30.05979200541871	0.0
36	3.961047063180681E-4	0.0	0.0	30.685241336694943	0.0
37	3.961047063180681E-4	0.0	0.0	31.332674479171825	0.0
38	3.961047063180681E-4	0.0	0.0	31.904847727448274	0.0
39	3.961047063180681E-4	0.0	0.0	32.555053602869386	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCAATC	35	1.2101191E-7	45.000004	27
GGGTACG	35	1.2101191E-7	45.000004	7
CTACGAA	70	0.0	45.000004	11
TACGAAT	70	0.0	45.000004	12
TTGCGTA	35	1.2101191E-7	45.000004	36
GCGACCG	20	7.029526E-4	45.0	33
CAACGTC	25	3.8876875E-5	45.0	19
ACCGCGA	25	3.8876875E-5	45.0	11
GACAACG	20	7.029526E-4	45.0	26
ACCGAAC	20	7.029526E-4	45.0	11
TTACTCG	20	7.029526E-4	45.0	39
CGCGACT	25	3.8876875E-5	45.0	13
TCGGCAC	20	7.029526E-4	45.0	27
TACGCTT	20	7.029526E-4	45.0	39
GCCGATT	45	3.8380676E-10	45.0	9
CGTACTA	25	3.8876875E-5	45.0	11
ACGTATA	20	7.029526E-4	45.0	14
CAATCCG	20	7.029526E-4	45.0	13
TTCGATT	20	7.029526E-4	45.0	37
CCGCACG	20	7.029526E-4	45.0	39
>>END_MODULE