FastQCFastQC Report
Sat 18 Jun 2016
SRR3549652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317305
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC37320.2833056885079765No Hit
CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT30090.2284209048018492No Hit
TCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC24860.1887186338774999No Hit
GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC19850.15068643935914613No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA253.8911166E-545.023
TCACACG207.0336624E-445.043
CGCCTAT253.8911166E-545.038
CCGCGTA207.0336624E-445.019
CGGTCAA253.8911166E-545.011
CGTCACA207.0336624E-445.041
ACGAAGC207.0336624E-445.016
CTACGCG302.1657452E-644.9999961
TAGGGCG6250.041.765
TAGGGAC18000.041.755
AGGGCGA9150.041.0655756
TCGTTAG2150.040.8139531
TTAGGGA45600.040.756584
CTTCGCG950.040.263161
GTTTACG1900.040.263161
TCTACGG3900.039.8076932
TAGGCGT403.4587356E-739.3754
CCGTTAG800.039.3751
TTATGGG40100.039.332923
TATGGGA36200.039.281774
Produced by FastQC (version 0.11.5)