##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549652_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1317305
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05731778137941	33.0	31.0	34.0	30.0	34.0
2	32.281007056072816	34.0	31.0	34.0	30.0	34.0
3	32.35466198033105	34.0	31.0	34.0	30.0	34.0
4	35.741589836825945	37.0	35.0	37.0	35.0	37.0
5	29.325487263769592	37.0	35.0	37.0	0.0	37.0
6	23.49994875901936	35.0	10.0	37.0	0.0	37.0
7	30.391553968139497	35.0	19.0	37.0	17.0	37.0
8	34.399144465404746	35.0	32.0	37.0	32.0	37.0
9	37.048389704738085	38.0	37.0	39.0	34.0	39.0
10	37.03471557460117	39.0	37.0	39.0	34.0	39.0
11	37.164438759436884	39.0	37.0	39.0	34.0	39.0
12	37.31388099187356	39.0	37.0	39.0	34.0	39.0
13	37.36202853553277	39.0	37.0	39.0	35.0	39.0
14	38.567642269633836	40.0	38.0	41.0	35.0	41.0
15	38.413973225638706	40.0	38.0	41.0	34.0	41.0
16	38.59506416509464	40.0	38.0	41.0	34.0	41.0
17	38.58855997661893	40.0	38.0	41.0	34.0	41.0
18	38.65524005450522	40.0	38.0	41.0	35.0	41.0
19	38.67256406071487	40.0	38.0	41.0	35.0	41.0
20	38.65191508420601	40.0	38.0	41.0	35.0	41.0
21	38.52447231279013	40.0	38.0	41.0	34.0	41.0
22	38.59549990321148	40.0	38.0	41.0	35.0	41.0
23	38.51978926672259	40.0	38.0	41.0	34.0	41.0
24	38.5184630742311	40.0	38.0	41.0	34.0	41.0
25	38.29043995126413	40.0	38.0	41.0	34.0	41.0
26	38.186407855432115	40.0	37.0	41.0	34.0	41.0
27	37.29734040332345	40.0	37.0	41.0	33.0	41.0
28	37.69359411829455	40.0	37.0	41.0	33.0	41.0
29	37.99230474339656	40.0	37.0	41.0	33.0	41.0
30	38.03189010897248	40.0	37.0	41.0	34.0	41.0
31	38.094104250724016	40.0	37.0	41.0	34.0	41.0
32	38.08621769445952	40.0	37.0	41.0	34.0	41.0
33	36.855894420806116	40.0	37.0	41.0	32.0	41.0
34	37.39297201483331	40.0	37.0	41.0	32.0	41.0
35	37.71207351372689	40.0	37.0	41.0	33.0	41.0
36	37.879348366551405	40.0	37.0	41.0	33.0	41.0
37	37.991157704555896	40.0	37.0	41.0	34.0	41.0
38	37.930057959242546	40.0	37.0	41.0	34.0	41.0
39	37.97067345831072	40.0	37.0	41.0	34.0	41.0
40	37.944186046511625	40.0	37.0	41.0	34.0	41.0
41	37.86770413837342	40.0	37.0	41.0	33.0	41.0
42	37.919943369227326	40.0	37.0	41.0	34.0	41.0
43	37.843141110069425	40.0	37.0	41.0	33.0	41.0
44	37.87037550149738	40.0	37.0	41.0	33.0	41.0
45	37.801397550301566	40.0	37.0	41.0	33.0	41.0
46	37.76942469663442	40.0	37.0	41.0	33.0	41.0
47	37.736500658541495	40.0	37.0	41.0	33.0	41.0
48	37.70247512914625	40.0	36.0	41.0	33.0	41.0
49	37.72597538155552	40.0	36.0	41.0	33.0	41.0
50	37.64536914382015	40.0	36.0	41.0	33.0	41.0
51	36.964434204683045	39.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	14.0
11	4.0
12	6.0
13	15.0
14	13.0
15	20.0
16	47.0
17	64.0
18	111.0
19	231.0
20	408.0
21	639.0
22	1068.0
23	1693.0
24	2648.0
25	3889.0
26	5359.0
27	7038.0
28	8643.0
29	11656.0
30	16108.0
31	23220.0
32	33937.0
33	53783.0
34	77729.0
35	105727.0
36	147652.0
37	235407.0
38	297646.0
39	282344.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.91846231510546	3.8954532169846767	56.6295580750092	17.556526392900658
2	18.56479706673853	4.18111219497383	56.10029567943643	21.153795058851216
3	20.623014412000256	4.2940700900702575	54.020595078588485	21.062320419341003
4	18.362186433665702	4.404522870557692	53.01277988013406	24.220510815642545
5	14.8576070082479	22.703398225923383	42.04614724760021	20.392847518228503
6	16.04601819624157	29.635429911827554	40.302663392304744	14.015888499626131
7	85.83084403384183	1.5072439564110058	8.399421546263014	4.262490463484159
8	87.77375019452595	2.0155544843449316	5.850809038149859	4.359886282979264
9	82.48833793236949	5.206994583638565	7.840856901021404	4.463810582970535
10	35.61460709554735	39.19881880050558	13.577873005871838	11.608701098075237
11	23.505945851568164	24.921031955393776	32.035253794679285	19.53776839835877
12	22.866002937816223	22.936146146868037	33.43288000880586	20.764970906509884
13	22.37720193880688	22.691404040825777	34.647632856475916	20.28376116389143
14	19.835421561445525	25.969688113231182	32.128626248287226	22.066264077036067
15	17.441442946014778	26.696702737786616	35.19078725124402	20.671067064954585
16	19.832233233761354	25.66748019630989	33.416179244745905	21.084107325182856
17	19.512185864321474	25.319117440531997	31.339591059018225	23.829105636128308
18	19.98413427414304	24.79160103392912	32.35249239925454	22.8717722926733
19	21.660815073198687	25.7947855659851	31.2898683296579	21.25453103115831
20	24.527956699473545	26.63369530974224	30.158467477159807	18.679880513624408
21	24.042419940712286	26.460159188646514	30.327372931857088	19.17004793878411
22	22.045995422472398	24.296954767498796	30.2275479103169	23.429501899711912
23	20.42457896994242	26.92899518334782	29.90674141523793	22.73968443147183
24	21.248685763737328	24.735046173816997	30.861114168700492	23.155153893745183
25	20.515901784324814	26.672258892207957	28.359263799955208	24.452575523512017
26	19.659683975996447	28.15915828149138	28.538797013599737	23.64236072891244
27	19.968420373413903	29.911068431380734	29.031165903112793	21.089345292092567
28	19.165720922641302	27.076948770406243	31.440858419272683	22.31647188767977
29	19.761103161378724	25.63445823100952	30.677557589168796	23.92688101844296
30	22.134433559426252	25.89795074033728	30.12628055006244	21.84133515017403
31	21.804137993858674	27.730631858225696	27.16250222993156	23.302727917984065
32	20.676532769556026	29.37747901966515	27.320248537734237	22.62573967304459
33	20.822360804825003	30.024557714424528	26.964142700437638	22.188938780312835
34	20.951336250906206	26.151726441484698	28.54411089307336	24.352826414535738
35	18.978824190297615	26.79227665574791	28.231882517716095	25.997016636238378
36	20.497530943858862	27.816261230314925	28.036255840522884	23.64995198530333
37	20.816439624840108	28.822558177491164	28.446031860503073	21.914970337165652
38	21.032562694288718	28.62032710723788	27.90674900649432	22.44036119197908
39	22.009709216923948	25.636431957671153	29.212369193163312	23.141489632241587
40	22.266369595499903	24.697621279809916	29.14435153590095	23.891657588789233
41	18.38139231233465	27.01447273030923	28.687965201680704	25.916169755675412
42	19.876414345956327	27.6393090438433	28.592770846538958	23.891505763661414
43	21.255897457308674	25.91366464106642	29.73980968720228	23.09062821442263
44	20.774156326742858	26.137682617161552	28.45529319329996	24.63286786279563
45	21.54740170271881	24.58663711137512	27.239857132554725	26.62610405335135
46	21.839437336076305	25.50867111261249	27.621545503888623	25.03034604742258
47	19.079787900296438	26.913888583129953	29.768884199179386	24.237439317394223
48	19.486223767464633	25.924444225141478	29.804563104216562	24.78476890317732
49	20.98253631467276	24.880190996010796	30.85898861691104	23.278284072405402
50	21.237982092226172	23.93902702866838	28.880631288881464	25.94235959022398
51	19.377365150819287	23.562576624244194	27.878661357848028	29.181396867088488
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	194.0
1	639.0
2	1084.0
3	2254.0
4	3424.0
5	2525.0
6	1626.0
7	1612.0
8	1598.0
9	1636.0
10	1674.0
11	1808.0
12	1942.0
13	1919.5
14	1897.0
15	1945.0
16	1993.0
17	2249.5
18	2506.0
19	2952.5
20	3399.0
21	4139.5
22	4880.0
23	6651.0
24	8422.0
25	9980.0
26	14706.0
27	17874.0
28	21193.0
29	24512.0
30	27710.5
31	30909.0
32	33959.5
33	37010.0
34	42002.5
35	46995.0
36	50503.0
37	54011.0
38	59186.5
39	64362.0
40	69103.5
41	73845.0
42	81056.0
43	88267.0
44	95433.0
45	102599.0
46	115454.0
47	128309.0
48	136165.5
49	144022.0
50	134835.0
51	125648.0
52	107082.0
53	88516.0
54	75405.5
55	62295.0
56	55936.5
57	49578.0
58	45771.0
59	41964.0
60	37606.5
61	33249.0
62	27948.0
63	22647.0
64	18061.5
65	13476.0
66	10845.0
67	8214.0
68	6373.5
69	4533.0
70	3799.0
71	3065.0
72	2679.0
73	2293.0
74	1944.0
75	1144.5
76	694.0
77	524.5
78	355.0
79	271.5
80	188.0
81	131.5
82	75.0
83	48.5
84	22.0
85	12.5
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1317305.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.434533128245896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.75834178495494	26.488054896486414
2	11.338756922703835	8.489221433121683
3	4.344847301143993	4.879419906955337
4	2.3321239179553337	3.4920788026349423
5	1.5746501999045828	2.9473147536863564
6	1.21099213356062	2.7199755085092123
7	0.9685878229653246	2.5381043062487714
8	0.8565069915319655	2.565035147926207
9	0.7799299867789767	2.6276683435010986
>10	5.713514599838309	36.96669308380083
>50	0.08362558957946659	2.1530807563502
>100	0.03344100865653098	2.123301915484634
>500	0.0033441003043791556	0.8830538164151476
>1k	0.0013376401217516623	1.1269973288791373
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	3732	0.2833056885079765	No Hit
CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT	3009	0.2284209048018492	No Hit
TCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	2486	0.1887186338774999	No Hit
GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	1985	0.15068643935914613	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2493727724407028	0.0
2	0.0	0.0	0.0	0.9426063060566839	0.0
3	0.0	0.0	0.0	1.3133632681877014	0.0
4	0.0	0.0	0.0	1.715775769468726	0.0
5	0.0	0.0	0.0	2.3987611069570067	0.0
6	0.0	0.0	0.0	2.921343196905804	0.0
7	0.0	0.0	0.0	3.6060745233639895	0.0
8	0.0	0.0	0.0	4.739373189959804	0.0
9	0.0	0.0	0.0	5.220962495397801	0.0
10	0.0	0.0	0.0	6.167895817597292	0.0
11	0.0	0.0	0.0	8.01553171057576	0.0
12	0.0	0.0	0.0	9.918052387260353	0.0
13	0.0	0.0	0.0	10.460068093569827	0.0
14	0.0	0.0	0.0	10.635122465943725	0.0
15	0.0	0.0	0.0	10.894591609384312	0.0
16	0.0	0.0	0.0	11.546604620797766	0.0
17	0.0	0.0	0.0	12.576206725094037	0.0
18	0.0	0.0	0.0	13.618182577307458	0.0
19	0.0	0.0	0.0	14.444035360072268	0.0
20	0.0	0.0	0.0	15.24734211135614	0.0
21	0.0	0.0	0.0	16.327805633471367	0.0
22	0.0	0.0	0.0	17.549694262148858	0.0
23	0.0	0.0	0.0	18.686257168992753	0.0
24	0.0	0.0	0.0	19.526457426336346	0.0
25	7.591256390888975E-5	0.0	0.0	20.239655964260365	0.0
26	7.591256390888975E-5	0.0	0.0	20.861987163185443	0.0
27	1.518251278177795E-4	0.0	0.0	21.520300917403336	0.0
28	1.518251278177795E-4	0.0	0.0	22.224086297402653	0.0
29	1.518251278177795E-4	0.0	0.0	22.964157882950417	0.0
30	2.2773769172666924E-4	0.0	0.0	23.78431722342206	0.0
31	2.2773769172666924E-4	0.0	0.0	24.555361135044656	0.0
32	2.2773769172666924E-4	0.0	0.0	25.274253115261843	0.0
33	2.2773769172666924E-4	0.0	0.0	25.95260778635168	0.0
34	2.2773769172666924E-4	0.0	0.0	26.64462671894512	0.0
35	2.2773769172666924E-4	0.0	0.0	27.405877909823467	0.0
36	3.03650255635559E-4	0.0	0.0	28.149821036130586	0.0
37	3.03650255635559E-4	0.0	0.0	28.90014081780605	0.0
38	3.03650255635559E-4	0.0	0.0	29.640440141045545	0.0
39	3.03650255635559E-4	0.0	0.0	30.366999290217528	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGTA	25	3.8911166E-5	45.0	23
TCACACG	20	7.0336624E-4	45.0	43
CGCCTAT	25	3.8911166E-5	45.0	38
CCGCGTA	20	7.0336624E-4	45.0	19
CGGTCAA	25	3.8911166E-5	45.0	11
CGTCACA	20	7.0336624E-4	45.0	41
ACGAAGC	20	7.0336624E-4	45.0	16
CTACGCG	30	2.1657452E-6	44.999996	1
TAGGGCG	625	0.0	41.76	5
TAGGGAC	1800	0.0	41.75	5
AGGGCGA	915	0.0	41.065575	6
TCGTTAG	215	0.0	40.813953	1
TTAGGGA	4560	0.0	40.75658	4
CTTCGCG	95	0.0	40.26316	1
GTTTACG	190	0.0	40.26316	1
TCTACGG	390	0.0	39.807693	2
TAGGCGT	40	3.4587356E-7	39.375	4
CCGTTAG	80	0.0	39.375	1
TTATGGG	4010	0.0	39.33292	3
TATGGGA	3620	0.0	39.28177	4
>>END_MODULE