FastQCFastQC Report
Sat 18 Jun 2016
SRR3549650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137817
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC30080.26436588660566684No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT28370.249337107812592No Hit
TCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC22890.2011747055985277No Hit
GCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC19970.17551152777643506No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT207.033257E-445.034
ATCGCGA207.033257E-445.024
TCGCGAA207.033257E-445.025
TCTCGAG650.044.9999961
CGAATAT900.042.514
TAGGGCG4950.042.2727285
TCAAGCG700.041.78571317
AGGGCCG1350.041.6666646
TAATACG650.041.5384633
GCCGATC1150.041.0869569
CTACGAA1100.040.90909211
TTAGGGA23450.040.778254
TCTACGG2550.040.5882342
GTCGTAG501.0822987E-940.4999961
CGTTCAT950.040.26315717
CGCTAGG1850.040.1351362
TATGGGT9300.039.677424
TCGATGG1650.039.5454522
TAGGGTA8200.039.5121965
TGTTTAG6550.039.503821
Produced by FastQC (version 0.11.5)