##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549650_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1137817
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.912463076224032	33.0	31.0	34.0	30.0	34.0
2	32.13620907404266	33.0	31.0	34.0	30.0	34.0
3	32.210599771316474	34.0	31.0	34.0	30.0	34.0
4	35.65607386776608	37.0	35.0	37.0	33.0	37.0
5	29.511037363653383	37.0	35.0	37.0	0.0	37.0
6	23.73496704654615	35.0	10.0	37.0	0.0	37.0
7	30.44535105381621	35.0	19.0	37.0	17.0	37.0
8	34.35304359136838	35.0	32.0	37.0	32.0	37.0
9	36.96200707143591	38.0	37.0	39.0	34.0	39.0
10	36.82028744516913	38.0	37.0	39.0	33.0	39.0
11	37.00831416651359	39.0	37.0	39.0	34.0	39.0
12	37.12377210043443	39.0	37.0	39.0	34.0	39.0
13	37.12744843854504	39.0	37.0	39.0	33.0	39.0
14	38.28204096089266	40.0	38.0	41.0	34.0	41.0
15	38.14115538790509	40.0	38.0	41.0	33.0	41.0
16	38.3342119163275	40.0	38.0	41.0	34.0	41.0
17	38.335067941505535	40.0	38.0	41.0	34.0	41.0
18	38.37833500466244	40.0	38.0	41.0	34.0	41.0
19	38.37534682642288	40.0	38.0	41.0	34.0	41.0
20	38.355898180463114	40.0	38.0	41.0	34.0	41.0
21	38.20981493509062	40.0	37.0	41.0	34.0	41.0
22	38.286655059644914	40.0	37.0	41.0	34.0	41.0
23	38.235433290239115	40.0	37.0	41.0	34.0	41.0
24	38.21466457259823	40.0	37.0	41.0	34.0	41.0
25	37.97094523987601	40.0	37.0	41.0	34.0	41.0
26	37.87089048590415	40.0	37.0	41.0	33.0	41.0
27	37.08173897911527	40.0	36.0	41.0	33.0	41.0
28	37.43887637467185	40.0	36.0	41.0	33.0	41.0
29	37.723967035120765	40.0	36.0	41.0	33.0	41.0
30	37.71843890537758	40.0	36.0	41.0	33.0	41.0
31	37.75360273224956	40.0	37.0	41.0	33.0	41.0
32	37.74936215577725	40.0	37.0	41.0	33.0	41.0
33	36.54487496671258	40.0	36.0	41.0	31.0	41.0
34	37.030700015907655	40.0	36.0	41.0	31.0	41.0
35	37.32090397665002	40.0	36.0	41.0	31.0	41.0
36	37.448075569269925	40.0	36.0	41.0	33.0	41.0
37	37.59604312468525	40.0	36.0	41.0	33.0	41.0
38	37.52487965991016	40.0	36.0	41.0	33.0	41.0
39	37.50416191707454	40.0	36.0	41.0	33.0	41.0
40	37.45302188313235	40.0	36.0	41.0	33.0	41.0
41	37.389851795148076	40.0	36.0	41.0	33.0	41.0
42	37.445133092579916	40.0	36.0	41.0	33.0	41.0
43	37.37906886608303	40.0	36.0	41.0	33.0	41.0
44	37.41209087225802	40.0	36.0	41.0	33.0	41.0
45	37.332502502599276	39.0	36.0	41.0	33.0	41.0
46	37.26540120247808	39.0	36.0	41.0	33.0	41.0
47	37.22603634855166	39.0	35.0	41.0	32.0	41.0
48	37.18473269427333	39.0	35.0	41.0	32.0	41.0
49	37.21275038077301	39.0	35.0	41.0	32.0	41.0
50	37.13727163506961	39.0	35.0	41.0	32.0	41.0
51	36.41318946719903	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	10.0
11	7.0
12	9.0
13	15.0
14	21.0
15	24.0
16	44.0
17	52.0
18	136.0
19	254.0
20	387.0
21	725.0
22	1234.0
23	2056.0
24	3206.0
25	4613.0
26	5938.0
27	7236.0
28	9129.0
29	12137.0
30	16543.0
31	23099.0
32	33863.0
33	52938.0
34	74712.0
35	99194.0
36	137031.0
37	209337.0
38	241868.0
39	201874.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.96184975264036	4.436390034601347	56.52727987013729	15.074480342621003
2	20.61974816688448	3.847806809003557	53.97607875431638	21.556366269795582
3	19.999525406985484	3.7093838464357627	53.46553971332823	22.825551033250512
4	18.612659153449105	4.7800305321506	52.25576696428335	24.351543350116934
5	14.245260881143453	22.283987671128134	43.23806025046207	20.232691197266345
6	15.800256104452648	29.265075139499586	41.53409555315134	13.400573202896423
7	84.28112780877768	1.602718187546855	9.39333829605288	4.722815707622579
8	86.06050006283962	1.9308904683266288	7.17233087570321	4.83627859313053
9	80.88813930535402	4.351666392750328	9.72432297988165	5.035871322013997
10	36.640338472706944	25.34467317679381	21.20507955145687	16.80990879904238
11	26.696999605384697	26.76027867398712	25.39406600534181	21.148655715286377
12	23.38144007340372	23.228867207995663	31.498298935593333	21.891393783007285
13	22.964501321389996	24.434685015252892	32.71967284721533	19.881140816141787
14	20.856868898952996	26.223021804033515	31.28675349375163	21.63335580326186
15	18.991191026324973	26.10806482940578	33.236803457849554	21.663940686419696
16	20.24473179781986	24.85127221688549	31.635139921446065	23.26885606384858
17	20.111230540587812	25.75510824675673	29.20381748558863	24.92984372706683
18	21.241904453879666	23.94690886144257	32.422700662760356	22.38848602191741
19	22.600295126544953	27.465664513713538	29.255934829590345	20.678105530151157
20	24.042970003084854	26.851769660674783	29.026372430716012	20.07888790552435
21	23.08587409047325	27.392454146844354	29.070755666332985	20.450916096349413
22	22.07077236497609	24.745982877738687	28.209984558149507	24.973260199135712
23	20.474382084289477	26.092332949850462	27.84208708430266	25.591197881557402
24	21.480870825449085	25.14130128131325	29.39673075723073	23.98109713600693
25	20.508746134044404	26.62651375396922	27.361957151281796	25.50278296070458
26	19.914977540325026	27.219051921354666	28.02972710022789	24.836243438092417
27	20.207291682230093	27.731085051462586	27.986398515754292	24.07522475055303
28	19.54347667507165	25.060181030868762	30.36463684406192	25.03170544999767
29	20.4700755921207	23.736857508720647	29.139044327866433	26.65402257129222
30	21.68810977512201	25.484150790504977	28.6056545121052	24.22208492226782
31	20.75447985045047	28.206469054338264	26.99502644098304	24.044024654228227
32	20.569212799597828	28.89190440993587	26.01217946295406	24.526703327512244
33	20.5395067923928	29.811032881386023	25.146047211458434	24.503413114762743
34	19.6397135919045	26.87514776101957	27.481132730483022	26.004005916592916
35	19.04752697490018	26.002951265449543	26.509974802626434	28.439546957023843
36	19.93984972978959	28.197152969238466	25.72900563095823	26.133991670013717
37	20.73549612986974	28.221849383512463	27.583346003794983	23.45930848282281
38	21.8740799267369	29.053002372086194	26.14866889842567	22.924248802751233
39	20.955654556048994	27.84129609594513	28.32555674594421	22.877492602061665
40	19.903903703319603	26.653583133315816	30.028378904516277	23.414134258848303
41	17.511163921790587	27.966799582006598	28.149342117405524	26.372694378797295
42	19.30266466400133	28.500453060553678	26.060693415549245	26.136188859895746
43	21.014890795268485	25.685325496103506	27.544587574276	25.75519613435201
44	21.390522377500073	24.63445351932692	26.56798061551198	27.40704348766102
45	21.991321978841942	23.649233576225352	26.10156114735498	28.25788329757773
46	21.189874997473233	25.24843625996096	27.66631189374038	25.895376848825425
47	18.92369335314906	26.50716239957744	29.095539968202267	25.47360427907124
48	19.26654286234078	25.9593590181901	28.64511604238643	26.128982077082696
49	21.751828281700835	24.7114430527932	28.907021076324224	24.62970758918174
50	21.216329163652855	23.685091715100057	28.11796624589016	26.980612875356936
51	19.21020691376557	24.74536766457172	26.475434977680944	29.568990443981765
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	122.0
1	543.5
2	965.0
3	2448.5
4	3932.0
5	2854.0
6	1776.0
7	1765.0
8	1754.0
9	1830.0
10	1906.0
11	1930.0
12	1954.0
13	1929.5
14	1905.0
15	2102.5
16	2300.0
17	2327.5
18	2355.0
19	2338.0
20	2321.0
21	2547.0
22	2773.0
23	3447.0
24	4121.0
25	5452.5
26	7855.5
27	8927.0
28	10415.5
29	11904.0
30	14517.0
31	17130.0
32	20046.0
33	22962.0
34	26904.5
35	30847.0
36	33905.0
37	36963.0
38	41599.0
39	46235.0
40	52124.0
41	58013.0
42	64838.5
43	71664.0
44	81653.0
45	91642.0
46	106046.5
47	120451.0
48	132063.5
49	143676.0
50	134312.0
51	124948.0
52	106005.5
53	87063.0
54	74303.5
55	61544.0
56	54124.5
57	46705.0
58	41294.5
59	35884.0
60	31874.5
61	27865.0
62	23943.0
63	20021.0
64	16920.0
65	13819.0
66	11173.0
67	8527.0
68	7281.5
69	6036.0
70	5032.5
71	4029.0
72	3348.5
73	2668.0
74	2186.0
75	1226.5
76	749.0
77	612.0
78	475.0
79	354.5
80	234.0
81	168.5
82	103.0
83	66.5
84	30.0
85	21.0
86	12.0
87	10.0
88	8.0
89	9.5
90	11.0
91	5.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1137817.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.66920426098797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.61710547923079	32.14800416275226
2	11.47944163307145	10.025961629533729
3	4.276336597570289	5.602326491041057
4	2.2859006003095756	3.992938409409356
5	1.453457573048439	3.1735667821066063
6	1.0556259195784286	2.765900634315819
7	0.8163052220286309	2.4953179636055594
8	0.6950111352738871	2.4280466583949214
9	0.5360653181800671	2.106859128915317
>10	3.661003936982333	27.897797650446666
>50	0.082785502015896	2.4701093697481196
>100	0.03671641114044214	2.839644519709701
>500	0.0027590365203094885	0.815126887538197
>1k	0.0014856350493974168	1.2383997124827049
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	3008	0.26436588660566684	No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT	2837	0.249337107812592	No Hit
TCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	2289	0.2011747055985277	No Hit
GCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	1997	0.17551152777643506	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2685844911791615	0.0
2	0.0	0.0	0.0	0.973970330905585	0.0
3	0.0	0.0	0.0	1.394336699135274	0.0
4	0.0	0.0	0.0	1.8729725430363582	0.0
5	0.0	0.0	0.0	2.6879542140783625	0.0
6	0.0	0.0	0.0	3.4776242576793983	0.0
7	0.0	0.0	0.0	4.341119881316591	0.0
8	0.0	0.0	0.0	5.795308032838321	0.0
9	8.788759528113924E-5	0.0	0.0	6.40234765344515	0.0
10	8.788759528113924E-5	0.0	0.0	7.656679413297569	0.0
11	8.788759528113924E-5	0.0	0.0	10.151280917757425	0.0
12	8.788759528113924E-5	0.0	0.0	12.16285219855214	0.0
13	8.788759528113924E-5	0.0	0.0	12.850748406817617	0.0
14	8.788759528113924E-5	0.0	0.0	13.063084837016849	0.0
15	8.788759528113924E-5	0.0	0.0	13.38246835826851	0.0
16	8.788759528113924E-5	0.0	0.0	14.260816985508214	0.0
17	8.788759528113924E-5	0.0	0.0	15.536769093799794	0.0
18	8.788759528113924E-5	0.0	0.0	16.99763670256289	0.0
19	8.788759528113924E-5	0.0	0.0	17.960181646081928	0.0
20	8.788759528113924E-5	0.0	0.0	18.91842009743219	0.0
21	8.788759528113924E-5	0.0	0.0	20.477897588100724	0.0
22	8.788759528113924E-5	0.0	0.0	22.049679342108618	0.0
23	8.788759528113924E-5	0.0	0.0	23.726662547668035	0.0
24	8.788759528113924E-5	0.0	0.0	24.930722603019642	0.0
25	8.788759528113924E-5	0.0	0.0	25.97368469622092	0.0
26	8.788759528113924E-5	0.0	0.0	26.85475783891434	0.0
27	8.788759528113924E-5	0.0	0.0	27.719308113694908	0.0
28	8.788759528113924E-5	0.0	0.0	28.694596758529713	0.0
29	8.788759528113924E-5	0.0	0.0	29.680783465179374	0.0
30	8.788759528113924E-5	0.0	0.0	30.708628891992298	0.0
31	8.788759528113924E-5	0.0	0.0	31.712744668079313	0.0
32	8.788759528113924E-5	0.0	0.0	32.62993961243328	0.0
33	8.788759528113924E-5	0.0	0.0	33.59362709469097	0.0
34	8.788759528113924E-5	0.0	0.0	34.48366477210307	0.0
35	8.788759528113924E-5	0.0	0.0	35.43329023911578	0.0
36	8.788759528113924E-5	0.0	0.0	36.37131454355138	0.0
37	8.788759528113924E-5	0.0	0.0	37.2938706312175	0.0
38	8.788759528113924E-5	0.0	0.0	38.18382042103431	0.0
39	8.788759528113924E-5	0.0	0.0	39.10707960946268	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGAGT	20	7.033257E-4	45.0	34
ATCGCGA	20	7.033257E-4	45.0	24
TCGCGAA	20	7.033257E-4	45.0	25
TCTCGAG	65	0.0	44.999996	1
CGAATAT	90	0.0	42.5	14
TAGGGCG	495	0.0	42.272728	5
TCAAGCG	70	0.0	41.785713	17
AGGGCCG	135	0.0	41.666664	6
TAATACG	65	0.0	41.53846	33
GCCGATC	115	0.0	41.086956	9
CTACGAA	110	0.0	40.909092	11
TTAGGGA	2345	0.0	40.77825	4
TCTACGG	255	0.0	40.588234	2
GTCGTAG	50	1.0822987E-9	40.499996	1
CGTTCAT	95	0.0	40.263157	17
CGCTAGG	185	0.0	40.135136	2
TATGGGT	930	0.0	39.67742	4
TCGATGG	165	0.0	39.545452	2
TAGGGTA	820	0.0	39.512196	5
TGTTTAG	655	0.0	39.50382	1
>>END_MODULE