SRR3549645_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549645_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	232814
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	910	0.3908699648646559	TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	754	0.323863685173572	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	725	0.31140738958997316	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	712	0.30582353294904946	TruSeq Adapter, Index 13 (95% over 22bp)
GCGGGCGGACTGACTGATCTTCCAGAGGTCCTGAGTTCAATTCCCAGCAAC	502	0.21562277182643658	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	477	0.20488458597850645	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	362	0.15548893107802797	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	238	0.10222752927229461	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTTCG	25	3.8811933E-5	45.000004	31
GGCGATA	25	3.8811933E-5	45.000004	8
CGAAGTG	25	3.8811933E-5	45.000004	12
GCTTTAT	25	3.8811933E-5	45.000004	9
CGGGTCC	20	7.021687E-4	45.0	6
CGGGTAT	20	7.021687E-4	45.0	6
CCGGGCG	35	1.2063538E-7	45.0	5
CCCTCTA	20	7.021687E-4	45.0	32
CGCGGGT	20	7.021687E-4	45.0	4
TTCCGCG	20	7.021687E-4	45.0	1
TCGGACT	20	7.021687E-4	45.0	14
TCTGTTG	105	0.0	45.0	1
GTATGCG	20	7.021687E-4	45.0	1
AACGCTC	20	7.021687E-4	45.0	20
ACGTGAG	35	1.2063538E-7	45.0	44
GATATGT	20	7.021687E-4	45.0	9
TTCGGAC	20	7.021687E-4	45.0	13
GGACCAA	45	3.8198777E-10	45.0	8
GTTATCA	20	7.021687E-4	45.0	45
GATAGTC	20	7.021687E-4	45.0	26