##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549645_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	232814
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.955526729492213	31.0	30.0	31.0	26.0	34.0
2	30.306618158701795	31.0	30.0	33.0	27.0	34.0
3	30.356318778080357	31.0	30.0	34.0	26.0	34.0
4	34.00066576752257	35.0	33.0	37.0	30.0	37.0
5	34.38702998960544	35.0	35.0	37.0	32.0	37.0
6	33.894868865274425	35.0	35.0	37.0	30.0	37.0
7	34.38778595788913	35.0	35.0	37.0	32.0	37.0
8	34.48018160419906	35.0	35.0	37.0	32.0	37.0
9	35.764460041062826	37.0	35.0	39.0	30.0	39.0
10	35.23847363131083	37.0	34.0	39.0	30.0	39.0
11	35.41675328803251	37.0	35.0	39.0	30.0	39.0
12	35.32419871657203	37.0	34.0	39.0	30.0	39.0
13	35.24333158658843	37.0	34.0	39.0	30.0	39.0
14	35.89825354145369	38.0	34.0	40.0	28.0	41.0
15	36.24204729956102	38.0	34.0	40.0	30.0	41.0
16	36.400139166888586	38.0	35.0	40.0	31.0	41.0
17	36.17832260946507	38.0	34.0	40.0	30.0	41.0
18	36.28824727035316	38.0	35.0	40.0	30.0	41.0
19	36.290536651575934	38.0	35.0	40.0	30.0	41.0
20	36.3389658697501	38.0	35.0	40.0	30.0	41.0
21	36.00113395242554	38.0	34.0	40.0	29.0	41.0
22	36.17083165101755	38.0	34.0	40.0	30.0	41.0
23	36.25991134553764	38.0	34.0	40.0	30.0	41.0
24	36.15851709948714	38.0	34.0	40.0	30.0	41.0
25	35.67851589681033	38.0	34.0	40.0	29.0	41.0
26	35.7772642538679	38.0	34.0	40.0	29.0	41.0
27	35.867250251273546	38.0	34.0	40.0	29.0	41.0
28	35.753979571675245	38.0	34.0	40.0	29.0	41.0
29	35.87590093379264	38.0	34.0	40.0	30.0	41.0
30	35.41346740316304	38.0	34.0	40.0	27.0	41.0
31	35.575884611750155	38.0	34.0	40.0	29.0	41.0
32	35.6524478768459	38.0	34.0	40.0	29.0	41.0
33	35.694197943422644	38.0	34.0	40.0	29.0	41.0
34	35.64007748674908	38.0	34.0	40.0	29.0	41.0
35	35.65536866339653	38.0	34.0	40.0	29.0	41.0
36	35.440849777075265	38.0	34.0	40.0	28.0	41.0
37	35.545035951446216	38.0	34.0	40.0	29.0	41.0
38	35.392759885573895	38.0	34.0	40.0	28.0	41.0
39	35.328279227194244	37.0	34.0	40.0	28.0	41.0
40	35.2180753734741	37.0	34.0	40.0	27.0	41.0
41	35.13446356318778	37.0	34.0	40.0	27.0	41.0
42	35.39474859759293	37.0	34.0	40.0	28.0	41.0
43	35.336100921765876	37.0	34.0	40.0	28.0	41.0
44	35.23706478132758	37.0	34.0	40.0	28.0	41.0
45	35.3032635494429	37.0	34.0	40.0	28.0	41.0
46	35.18339532845963	37.0	34.0	40.0	28.0	41.0
47	35.12534469576572	37.0	34.0	40.0	28.0	41.0
48	35.10498939067238	37.0	34.0	40.0	28.0	41.0
49	35.12320564914481	37.0	34.0	40.0	28.0	41.0
50	34.943633114847046	37.0	34.0	40.0	27.0	41.0
51	33.81062994493458	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	7.0
12	2.0
13	2.0
14	7.0
15	16.0
16	33.0
17	46.0
18	100.0
19	193.0
20	333.0
21	527.0
22	801.0
23	1129.0
24	1614.0
25	2066.0
26	2742.0
27	3593.0
28	4558.0
29	5753.0
30	7410.0
31	9627.0
32	11949.0
33	16164.0
34	22439.0
35	22841.0
36	26940.0
37	33844.0
38	39865.0
39	18211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.90314156365167	4.779351757196732	52.713324800054984	17.60418187909662
2	20.738873091824374	4.468373895040676	52.81898854879861	21.973764464336337
3	22.258541153023444	4.385045572860738	51.33239410001117	22.02401917410465
4	21.05887103009269	5.074437104297851	46.1651790699872	27.701512795622257
5	19.3463451510648	5.949384487187197	48.71957871949281	25.984691642255193
6	22.136555361790958	7.079900693257278	51.513225149690314	19.270318795261453
7	83.33433556401248	2.663070090286667	9.784205417199997	4.218388928500864
8	83.4348449835491	3.7351705653440086	7.314422672176072	5.51556177893082
9	76.64960010995902	6.303315092734973	10.176793491800321	6.870291305505683
10	38.808233181853325	28.21866382605857	17.14115130533387	15.831951686754234
11	31.29322119803792	25.001503346018712	24.51914403772969	19.18613141821368
12	28.134476449010798	22.529143436391283	29.30493870643518	20.03144140816274
13	23.907067444397672	25.656532682742444	30.845224084462274	19.591175788397607
14	19.56497461492866	28.439440927092015	30.10085304148376	21.89473141649557
15	17.953387682871305	25.281984760366644	35.39005386274021	21.374573694021837
16	20.215279149879304	23.730531669057704	33.31629541178795	22.737893769275043
17	19.68266513182197	24.026905598460573	29.025316346955083	27.265112922762373
18	21.062736776997948	23.590076198166777	32.29788586597026	23.049301158865017
19	22.914859072048934	26.03881209892876	28.95916912213183	22.087159706890482
20	27.17534168907368	25.210253678902472	28.588057419227365	19.026347212796484
21	24.03678472944067	26.7363646516103	28.237992560584846	20.988858058364187
22	22.41445961153539	24.617935347530647	28.509883426254433	24.457721614679528
23	21.95615383954573	26.3244478424837	27.9871485391772	23.73224977879337
24	22.785571314439853	24.802202616681125	29.880934995318153	22.53129107356087
25	21.24528593641276	26.145764429974143	27.742747429278307	24.86620220433479
26	20.23246024723599	27.65340572302353	28.146073689726563	23.968060340013917
27	20.640511309457334	26.86221618974804	29.153315522262407	23.34395697853222
28	18.471397768175454	26.520312352349944	32.29359059163109	22.714699287843516
29	21.62885393490082	23.507606930854674	31.140309431563395	23.72322970268111
30	21.003891518551292	24.946523834477308	30.730110732172466	23.319473914798937
31	23.41611758743031	26.25915967252828	26.97818859690568	23.34653414313572
32	23.249460943070435	26.787907943680363	28.544675148401726	21.417955964847472
33	21.859080639480442	25.23774343467317	28.957451012396163	23.945724913450224
34	19.762986761964488	25.60971419244547	30.50332024706418	24.12397879852586
35	20.583384160746345	25.164723770907248	28.71949281400603	25.532399254340376
36	22.88736931627823	24.69310264846616	29.49092408532133	22.92860394993428
37	21.096669444277406	25.214119425807723	31.058269691685208	22.63094143822966
38	20.774094341405586	26.46748047797813	28.8934514247425	23.864973755873788
39	22.05881089625194	24.760967983025076	30.2816840911629	22.898537029560078
40	21.67481337032996	23.82760486912299	30.282972673464652	24.21460908708239
41	18.955475186200143	24.632109752849914	29.578118154406525	26.834296906543422
42	21.07304543541196	23.358131383851486	30.321630142517204	25.24719303821935
43	21.805819237674708	23.48956677863015	29.432508354308588	25.272105629386548
44	20.44292868985542	24.21675672425198	29.759378731519583	25.58093585437302
45	21.78606097571452	24.65702234401711	27.37249478124168	26.184421899026688
46	22.26842028400354	24.645425103301346	29.315247364849196	23.770907247845923
47	19.3613786112519	24.841289613167593	31.360227477729	24.4371042978515
48	20.128514608228027	23.278668808576803	32.14755126409924	24.44526531909593
49	21.585042136641267	22.31437971943268	32.83049988402759	23.27007825989846
50	21.106548575257502	22.735746132105458	30.538111969211474	25.619593323425566
51	19.264305411186612	23.111153109349093	28.987517932770366	28.63702354669393
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	151.5
2	251.0
3	379.5
4	508.0
5	391.5
6	275.0
7	322.0
8	369.0
9	441.0
10	513.0
11	583.5
12	654.0
13	660.5
14	667.0
15	638.0
16	609.0
17	597.0
18	585.0
19	647.0
20	709.0
21	733.0
22	757.0
23	868.0
24	979.0
25	1221.0
26	1795.5
27	2128.0
28	2628.5
29	3129.0
30	3455.5
31	3782.0
32	4250.5
33	4719.0
34	5472.0
35	6225.0
36	7025.0
37	7825.0
38	8216.0
39	8607.0
40	9960.5
41	11314.0
42	12927.0
43	14540.0
44	15822.0
45	17104.0
46	19644.5
47	22185.0
48	23361.0
49	24537.0
50	23464.0
51	22391.0
52	19496.0
53	16601.0
54	14639.0
55	12677.0
56	11812.5
57	10948.0
58	10254.0
59	9560.0
60	8578.5
61	7597.0
62	6765.5
63	5934.0
64	5209.0
65	4484.0
66	3870.5
67	3257.0
68	2502.5
69	1748.0
70	1501.0
71	1254.0
72	1043.5
73	833.0
74	720.0
75	400.0
76	193.0
77	167.5
78	142.0
79	99.0
80	56.0
81	39.0
82	22.0
83	22.0
84	22.0
85	11.5
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	232814.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.5394091420619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.05520784466286	28.175281555232935
2	9.915672162660046	7.444569484652986
3	3.4188816549767154	3.8502839176338193
4	1.629346545075918	2.4465882635923957
5	1.0400814673272538	1.9522021871536934
6	0.8032312321933247	1.8091695516592645
7	0.6316006270238108	1.6596940046560773
8	0.5640925889904688	1.6940561993694536
9	0.5743904253006397	1.940604946437929
>10	6.234767783791206	42.887455221765016
>50	0.08695950661922035	2.167395431546213
>100	0.0400471412062199	2.4251118918965355
>500	0.005721020172317128	1.5475873444036872
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	910	0.3908699648646559	TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	754	0.323863685173572	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	725	0.31140738958997316	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	712	0.30582353294904946	TruSeq Adapter, Index 13 (95% over 22bp)
GCGGGCGGACTGACTGATCTTCCAGAGGTCCTGAGTTCAATTCCCAGCAAC	502	0.21562277182643658	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	477	0.20488458597850645	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	362	0.15548893107802797	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	238	0.10222752927229461	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.338467617926757	0.0
2	0.0	0.0	0.0	1.5020574364084633	0.0
3	0.0	0.0	0.0	1.955208879191114	0.0
4	0.0	0.0	0.0	2.626130730969787	0.0
5	0.0	0.0	0.0	3.7618012662468754	0.0
6	0.0	0.0	0.0	4.320616457773158	0.0
7	0.0	0.0	0.0	4.871270628055014	0.0
8	0.0	0.0	0.0	5.779721150789901	0.0
9	0.0	0.0	0.0	6.123343097923664	0.0
10	0.0	0.0	0.0	7.05498810209008	0.0
11	0.0	0.0	0.0	8.60987741287036	0.0
12	0.0	0.0	0.0	10.18151829357341	0.0
13	0.0	0.0	0.0	10.634240208922144	0.0
14	0.0	0.0	0.0	10.792735832037593	0.0
15	0.0	0.0	0.0	11.120465264116419	0.0
16	0.0	0.0	0.0	11.779789875179327	0.0
17	0.0	0.0	0.0	12.737206525380776	0.0
18	0.0	0.0	0.0	13.832931009303564	0.0
19	0.0	0.0	0.0	14.60006700627969	0.0
20	0.0	0.0	0.0	15.29332428462206	0.0
21	0.0	0.0	0.0	16.254606681728763	0.0
22	0.0	0.0	0.0	17.249392218681006	0.0
23	0.0	0.0	0.0	18.20509075914679	0.0
24	0.0	0.0	0.0	18.954186603898393	0.0
25	0.0	0.0	0.0	19.582155712285342	0.0
26	0.0	0.0	0.0	20.196809470220863	0.0
27	0.0	0.0	0.0	20.769799067066412	0.0
28	0.0	0.0	0.0	21.3586811789669	0.0
29	0.0	0.0	0.0	22.0102742962193	0.0
30	0.0	0.0	0.0	22.715987870145266	0.0
31	0.0	0.0	0.0	23.41611758743031	0.0
32	0.0	0.0	0.0	24.03979142147809	0.0
33	0.0	0.0	0.0	24.69868650510708	0.0
34	0.0	0.0	0.0	25.354145369264735	0.0
35	0.0	0.0	0.0	26.022919583873822	0.0
36	0.0	0.0	0.0	26.664204042712207	0.0
37	0.0	0.0	0.0	27.326535345812538	0.0
38	0.0	0.0	0.0	27.972544606424012	0.0
39	0.0	0.0	0.0	28.684701091858738	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTTCG	25	3.8811933E-5	45.000004	31
GGCGATA	25	3.8811933E-5	45.000004	8
CGAAGTG	25	3.8811933E-5	45.000004	12
GCTTTAT	25	3.8811933E-5	45.000004	9
CGGGTCC	20	7.021687E-4	45.0	6
CGGGTAT	20	7.021687E-4	45.0	6
CCGGGCG	35	1.2063538E-7	45.0	5
CCCTCTA	20	7.021687E-4	45.0	32
CGCGGGT	20	7.021687E-4	45.0	4
TTCCGCG	20	7.021687E-4	45.0	1
TCGGACT	20	7.021687E-4	45.0	14
TCTGTTG	105	0.0	45.0	1
GTATGCG	20	7.021687E-4	45.0	1
AACGCTC	20	7.021687E-4	45.0	20
ACGTGAG	35	1.2063538E-7	45.0	44
GATATGT	20	7.021687E-4	45.0	9
TTCGGAC	20	7.021687E-4	45.0	13
GGACCAA	45	3.8198777E-10	45.0	8
GTTATCA	20	7.021687E-4	45.0	45
GATAGTC	20	7.021687E-4	45.0	26
>>END_MODULE