FastQCFastQC Report
Sat 18 Jun 2016
SRR3549644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413639
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC40160.9708949107796896No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG35330.8541264242491641No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC23970.5794908120365826No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC17220.41630503893491666TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACCTAT16110.38947004513597605No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT13740.3321737070247245TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC12890.31162438744895915TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC11610.28067952973486543TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTA7510.18155928236940908No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT6540.15810888238294746No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC6160.1489221277490759No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT6140.14843861434729316No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.10081254427169585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCA302.16212E-645.00000440
TCGCTTT302.16212E-645.00000429
CGATCAA302.16212E-645.00000441
TCGAATC302.16212E-645.00000438
TCACGCC207.028044E-445.013
TCGTTGA253.8864608E-545.024
AGCCGAT351.2094097E-745.018
TCGTTCA351.2094097E-745.016
CTCACGC207.028044E-445.012
ACACGCG207.028044E-445.036
CTCGTAC351.2094097E-745.029
CGTTGAT253.8864608E-545.025
TAAACGA207.028044E-445.013
ACCGAAT207.028044E-445.039
TTTCCCG502.1827873E-1145.01
GCCGATT351.2094097E-745.09
AAGCCGA351.2094097E-745.017
TTACGTG207.028044E-445.013
GGGCACG207.028044E-445.07
GCTACCG207.028044E-445.01
Produced by FastQC (version 0.11.5)