##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549644_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413639
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.813697451159104	31.0	30.0	31.0	25.0	34.0
2	29.813649099818925	31.0	30.0	33.0	25.0	34.0
3	29.881268449058236	31.0	30.0	33.0	25.0	34.0
4	33.83571665147629	35.0	33.0	37.0	28.0	37.0
5	34.360925831461735	35.0	35.0	37.0	32.0	37.0
6	33.69519315151618	35.0	33.0	37.0	28.0	37.0
7	34.42437004247665	35.0	35.0	37.0	32.0	37.0
8	34.28569598127836	35.0	35.0	37.0	30.0	37.0
9	35.851745120745385	37.0	35.0	39.0	31.0	39.0
10	35.06613738066285	37.0	34.0	39.0	30.0	39.0
11	35.235618498255725	37.0	34.0	39.0	30.0	39.0
12	35.326697917749534	37.0	34.0	39.0	30.0	39.0
13	35.349734913777475	37.0	34.0	39.0	30.0	39.0
14	36.046117508262036	38.0	34.0	40.0	29.0	41.0
15	36.238091669305845	38.0	35.0	40.0	30.0	41.0
16	36.45910081012671	38.0	35.0	40.0	31.0	41.0
17	36.14891729261506	38.0	34.0	40.0	30.0	41.0
18	36.313481078911806	38.0	35.0	40.0	30.0	41.0
19	36.28162479843535	38.0	35.0	40.0	30.0	41.0
20	36.214271381567016	38.0	34.0	40.0	30.0	41.0
21	35.99254905847853	38.0	34.0	40.0	30.0	41.0
22	36.21259600762984	38.0	34.0	40.0	30.0	41.0
23	36.181327195936554	38.0	34.0	40.0	30.0	41.0
24	36.090842497926936	38.0	34.0	40.0	30.0	41.0
25	35.730006116444535	38.0	34.0	40.0	29.0	41.0
26	35.79900347887892	38.0	34.0	40.0	29.0	41.0
27	35.77907547402445	38.0	34.0	40.0	29.0	41.0
28	35.65771361017699	38.0	34.0	40.0	29.0	41.0
29	35.8158128222919	38.0	34.0	40.0	29.0	41.0
30	35.36320317958413	38.0	34.0	40.0	27.0	41.0
31	35.58954547322666	38.0	34.0	40.0	29.0	41.0
32	35.571466907133996	38.0	34.0	40.0	28.0	41.0
33	35.581376514303535	38.0	34.0	40.0	28.0	41.0
34	35.57147657740203	38.0	34.0	40.0	28.0	41.0
35	35.55840237501783	38.0	34.0	40.0	28.0	41.0
36	35.33013811560322	38.0	34.0	40.0	27.0	41.0
37	35.326448908347615	38.0	34.0	40.0	27.0	41.0
38	35.31877796822833	38.0	34.0	40.0	27.0	41.0
39	35.32960383329425	38.0	34.0	40.0	27.0	41.0
40	35.370874119703416	38.0	34.0	40.0	28.0	41.0
41	35.24436525569398	38.0	34.0	40.0	27.0	41.0
42	35.37261718551684	38.0	34.0	40.0	28.0	41.0
43	35.360812205812316	38.0	34.0	40.0	28.0	41.0
44	35.342204192544706	38.0	34.0	40.0	28.0	41.0
45	35.417683052129995	38.0	34.0	40.0	28.0	41.0
46	35.30734045870916	38.0	34.0	40.0	28.0	41.0
47	35.140513829692075	37.0	34.0	40.0	27.0	41.0
48	35.113644989955006	37.0	34.0	40.0	27.0	41.0
49	35.14988915455264	37.0	34.0	40.0	27.0	41.0
50	34.98535679662701	37.0	34.0	40.0	27.0	41.0
51	33.877654186379914	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	6.0
11	8.0
12	3.0
13	6.0
14	6.0
15	21.0
16	48.0
17	84.0
18	171.0
19	336.0
20	644.0
21	1300.0
22	1888.0
23	2496.0
24	3237.0
25	4102.0
26	4940.0
27	6061.0
28	7839.0
29	9936.0
30	13392.0
31	16908.0
32	21577.0
33	27870.0
34	37036.0
35	40357.0
36	49246.0
37	62222.0
38	71581.0
39	30315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.062781797654473	4.354038183053339	54.62323426949587	11.95994574979632
2	18.412190339885743	9.042667640140316	51.8166807288481	20.72846129112584
3	19.86466459884102	8.900998213417981	50.94321376852763	20.29112341921337
4	17.95647895870554	4.224698348076463	51.809428027821355	26.00939466539664
5	20.96030596728065	5.14168151455738	50.54238115844976	23.355631359712213
6	19.35721728367006	10.332923152797488	53.2909130908836	17.018946472648857
7	80.04564366512828	1.9277679329076802	13.831867884798097	4.194720517165935
8	82.04521333820071	6.428794190102964	6.722528581685963	4.803463890010371
9	77.55119802533127	3.9916448884171944	8.358737933318666	10.098419152932872
10	40.12073329642514	21.736344977141904	20.896965711647113	17.24595601478584
11	30.039720625956452	27.054750640050866	23.68998087704496	19.215547856947726
12	23.52268524002814	22.28416566136172	30.30105961962001	23.892089478990133
13	23.580223334840284	25.16711431949115	32.71935189863625	18.533310447032317
14	20.969976235316302	29.18075906768946	29.885238094086873	19.964026602907367
15	18.180103907030045	26.426666731135118	34.434857448161324	20.958371913673517
16	19.282997976496414	25.32570671527588	30.509212139087467	24.88208316914024
17	19.52282062378064	27.640043612908844	29.000650325525395	23.83648543778512
18	21.34856722891217	24.992807738148482	30.240136930995387	23.418488101943964
19	21.492412465942525	28.961243983280106	27.424154879012857	22.12218867176451
20	24.923181808291773	26.471875234201804	30.094841153759678	18.510101803746746
21	22.32526430051325	30.37745473710168	28.20236969918214	19.09491126320294
22	21.488786115429154	24.71043591150738	29.881853500274392	23.918924472789076
23	21.120832416672506	28.5529169154746	27.01244321739488	23.313807450458008
24	21.370808845394173	26.880202302007305	28.937793583293647	22.811195269304875
25	19.512425085642313	29.91860051880988	26.44068861978682	24.12828577576099
26	19.672951535034173	28.374258713515893	29.375614968607895	22.577174782842043
27	21.356061686639798	27.772526284997305	29.326296601626055	21.545115426736842
28	18.94357156844495	28.851002927673647	30.776353293572416	21.429072210308988
29	20.777779658107672	24.960895853630824	30.536772402989083	23.724552085272425
30	19.615655196922923	28.56621353402363	29.96187496826943	21.856256300784015
31	21.863025488408976	27.604505377877814	25.760627020179435	24.771842113533783
32	21.45614896080882	30.258752196964018	26.31038175800638	21.97471708422078
33	20.79301033026383	27.656724825270345	26.250909609587104	25.299355234878725
34	20.831207889004663	26.731763687660013	27.24428789354969	25.192740529785635
35	19.592446553637348	26.48662239295618	29.396889558286333	24.52404149512014
36	22.38594523243698	28.901771834860835	26.276294063180693	22.43598886952149
37	18.726232294343617	29.057946663636645	30.409608378320225	21.806212663699505
38	19.67947896595824	29.38528523664355	26.30167851677429	24.633557280623926
39	19.18774583634522	29.51196574791062	27.04653091222056	24.253757503523605
40	18.82293497470016	25.13254311126369	29.128539620296927	26.915982293739226
41	17.690546587725045	27.00567402976992	28.198743348668764	27.105036033836267
42	20.019388887411488	26.27000838895752	29.44330684485747	24.26729587877352
43	20.24857423985649	28.743179439076105	27.54019809544071	23.468048225626696
44	19.729280846341858	25.8957690159777	28.679597426741676	25.695352710938767
45	20.887053686910566	24.690370105333397	26.77382935361511	27.64874685414093
46	22.25177026344228	27.350419085240997	26.926377831877556	23.471432819439173
47	18.021995024647094	26.395480116720133	31.66239160233924	23.920133256293532
48	19.048010463230014	26.421831597117297	29.333307545951904	25.196850393700785
49	20.53481417371186	24.25133993651469	31.258657911850673	23.95518797792278
50	19.97369687094302	25.153817700942128	27.516022425351576	27.35646300276328
51	18.48761843056385	24.517272307495183	27.311254499696595	29.683854762244373
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	99.0
1	215.5
2	332.0
3	597.0
4	862.0
5	693.5
6	525.0
7	519.0
8	513.0
9	598.5
10	684.0
11	678.5
12	673.0
13	682.0
14	691.0
15	758.5
16	826.0
17	845.0
18	864.0
19	897.0
20	930.0
21	1130.0
22	1330.0
23	1717.5
24	2105.0
25	2336.0
26	3171.5
27	3776.0
28	4508.5
29	5241.0
30	6127.0
31	7013.0
32	8188.0
33	9363.0
34	11075.0
35	12787.0
36	14172.5
37	15558.0
38	17036.0
39	18514.0
40	20638.5
41	22763.0
42	25290.0
43	27817.0
44	30148.0
45	32479.0
46	42384.5
47	52290.0
48	50505.0
49	48720.0
50	45210.5
51	41701.0
52	35560.5
53	29420.0
54	24647.5
55	19875.0
56	17328.5
57	14782.0
58	13302.0
59	11822.0
60	10187.0
61	8552.0
62	7495.5
63	6439.0
64	5436.0
65	4433.0
66	3610.5
67	2788.0
68	2224.5
69	1661.0
70	1428.0
71	1195.0
72	931.5
73	668.0
74	596.0
75	397.5
76	271.0
77	180.0
78	89.0
79	74.5
80	60.0
81	35.0
82	10.0
83	9.5
84	9.0
85	13.0
86	17.0
87	9.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	413639.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.18312216806462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.1622722545599	32.12762435111499
2	10.517398001407274	8.873133695670436
3	3.7201872734354846	4.707873427302248
4	1.8757218393524535	3.1649521401084577
5	1.1879598695191012	2.5055928153341176
6	0.827130442147918	2.093456669403056
7	0.6546731833194575	1.933131121048431
8	0.5366189615894719	1.8109010571542932
9	0.4611142399440381	1.7506114485294262
>10	3.912641708357187	30.19524330387916
>50	0.0909731306816482	2.587522282629817
>100	0.04635573537918379	3.425423887093733
>500	0.00231778676895919	0.6440696405374616
>1k	0.00463557353791838	4.1804641601943855
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC	4016	0.9708949107796896	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG	3533	0.8541264242491641	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC	2397	0.5794908120365826	No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	1722	0.41630503893491666	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACCTAT	1611	0.38947004513597605	No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT	1374	0.3321737070247245	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	1289	0.31162438744895915	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	1161	0.28067952973486543	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTA	751	0.18155928236940908	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT	654	0.15810888238294746	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC	616	0.1489221277490759	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT	614	0.14843861434729316	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	417	0.10081254427169585	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3611845111316873	0.0
2	0.0	0.0	0.0	1.4930893847050206	0.0
3	0.0	0.0	0.0	1.8982736153989348	0.0
4	0.0	0.0	0.0	2.760619767478405	0.0
5	0.0	0.0	0.0	4.433817894347486	0.0
6	0.0	0.0	0.0	5.041352483687467	0.0
7	0.0	0.0	0.0	5.7441392131786415	0.0
8	0.0	0.0	0.0	6.766044787846407	0.0
9	0.0	0.0	0.0	7.117075517540657	0.0
10	0.0	0.0	0.0	8.878998353636867	0.0
11	0.0	0.0	0.0	10.569602962970126	0.0
12	0.0	0.0	0.0	13.0289938811379	0.0
13	0.0	0.0	0.0	13.583825509683564	0.0
14	0.0	0.0	0.0	13.792703299253697	0.0
15	0.0	0.0	0.0	14.25300805775084	0.0
16	0.0	0.0	0.0	15.020343826380008	0.0
17	0.0	0.0	0.0	16.141853161815014	0.0
18	0.0	0.0	0.0	17.309054513718483	0.0
19	0.0	0.0	0.0	18.604386917094374	0.0
20	0.0	0.0	0.0	19.420315782602703	0.0
21	0.0	0.0	0.0	20.699692243719767	0.0
22	0.0	0.0	0.0	21.970365463604736	0.0
23	0.0	0.0	0.0	23.46393836171154	0.0
24	0.0	0.0	0.0	24.480283532258806	0.0
25	0.0	0.0	0.0	25.405244669869138	0.0
26	0.0	0.0	0.0	26.21633840135964	0.0
27	0.0	0.0	0.0	27.045805642117884	0.0
28	0.0	0.0	0.0	27.97850299415674	0.0
29	0.0	0.0	0.0	28.884123595695765	0.0
30	0.0	0.0	0.0	29.858161343587042	0.0
31	0.0	0.0	0.0	30.80608936778205	0.0
32	0.0	0.0	0.0	31.722347264160295	0.0
33	0.0	0.0	0.0	32.629418405904666	0.0
34	0.0	0.0	0.0	33.47750091263155	0.0
35	0.0	0.0	0.0	34.39738515952316	0.0
36	0.0	0.0	0.0	35.273269686852544	0.0
37	0.0	0.0	0.0	36.22506581826182	0.0
38	0.0	0.0	0.0	37.16380708782296	0.0
39	0.0	0.0	0.0	38.03171364402293	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCA	30	2.16212E-6	45.000004	40
TCGCTTT	30	2.16212E-6	45.000004	29
CGATCAA	30	2.16212E-6	45.000004	41
TCGAATC	30	2.16212E-6	45.000004	38
TCACGCC	20	7.028044E-4	45.0	13
TCGTTGA	25	3.8864608E-5	45.0	24
AGCCGAT	35	1.2094097E-7	45.0	18
TCGTTCA	35	1.2094097E-7	45.0	16
CTCACGC	20	7.028044E-4	45.0	12
ACACGCG	20	7.028044E-4	45.0	36
CTCGTAC	35	1.2094097E-7	45.0	29
CGTTGAT	25	3.8864608E-5	45.0	25
TAAACGA	20	7.028044E-4	45.0	13
ACCGAAT	20	7.028044E-4	45.0	39
TTTCCCG	50	2.1827873E-11	45.0	1
GCCGATT	35	1.2094097E-7	45.0	9
AAGCCGA	35	1.2094097E-7	45.0	17
TTACGTG	20	7.028044E-4	45.0	13
GGGCACG	20	7.028044E-4	45.0	7
GCTACCG	20	7.028044E-4	45.0	1
>>END_MODULE