##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549643_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	636003
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.052449438131582	31.0	30.0	31.0	26.0	34.0
2	30.297662117945986	31.0	30.0	33.0	26.0	34.0
3	30.333918236234734	31.0	30.0	34.0	26.0	34.0
4	33.98497963059923	35.0	33.0	37.0	28.0	37.0
5	34.41564112118968	35.0	35.0	37.0	32.0	37.0
6	33.864360702701084	35.0	35.0	37.0	29.0	37.0
7	34.29529105994783	35.0	35.0	37.0	31.0	37.0
8	34.3704730952527	35.0	35.0	37.0	31.0	37.0
9	35.8088719707297	37.0	35.0	39.0	30.0	39.0
10	35.319029941682665	37.0	34.0	39.0	30.0	39.0
11	35.50136398727679	37.0	35.0	39.0	30.0	39.0
12	35.53380565814941	37.0	35.0	39.0	30.0	39.0
13	35.50502120273018	37.0	35.0	39.0	30.0	39.0
14	36.252245665507864	38.0	35.0	40.0	30.0	41.0
15	36.49315647882164	38.0	35.0	40.0	30.0	41.0
16	36.6280473519779	38.0	35.0	40.0	31.0	41.0
17	36.37968846058902	38.0	35.0	40.0	30.0	41.0
18	36.51101645746954	38.0	35.0	40.0	30.0	41.0
19	36.495728793732106	38.0	35.0	40.0	30.0	41.0
20	36.49829324704443	38.0	35.0	40.0	30.0	41.0
21	36.20748329803476	38.0	34.0	40.0	30.0	41.0
22	36.36821209962846	38.0	35.0	40.0	30.0	41.0
23	36.42263322657283	38.0	35.0	40.0	30.0	41.0
24	36.345826985092835	38.0	35.0	40.0	30.0	41.0
25	35.895469046529655	38.0	34.0	40.0	29.0	41.0
26	35.99335223261526	38.0	34.0	40.0	30.0	41.0
27	36.01238201706596	38.0	34.0	40.0	30.0	41.0
28	35.85935915396625	38.0	34.0	40.0	29.0	41.0
29	35.97369980959209	38.0	34.0	40.0	30.0	41.0
30	35.498895445461734	38.0	34.0	40.0	27.0	41.0
31	35.72037238818056	38.0	34.0	40.0	29.0	41.0
32	35.71939126073305	38.0	34.0	40.0	29.0	41.0
33	35.69735362883508	38.0	34.0	40.0	29.0	41.0
34	35.648647883736395	38.0	34.0	40.0	28.0	41.0
35	35.6297501741344	38.0	34.0	40.0	28.0	41.0
36	35.40552009974796	38.0	34.0	40.0	27.0	41.0
37	35.47603863503789	38.0	34.0	40.0	28.0	41.0
38	35.354745182019585	38.0	34.0	40.0	27.0	41.0
39	35.279547423518444	38.0	34.0	40.0	27.0	41.0
40	35.18639063023288	38.0	34.0	40.0	27.0	41.0
41	35.05360981001662	37.0	33.0	40.0	26.0	41.0
42	35.28159930063223	38.0	34.0	40.0	28.0	41.0
43	35.256921744079825	37.0	34.0	40.0	27.0	41.0
44	35.20612009691778	37.0	34.0	40.0	27.0	41.0
45	35.23472530789949	37.0	34.0	40.0	28.0	41.0
46	35.10209857500672	37.0	34.0	40.0	27.0	41.0
47	35.0316948190496	37.0	34.0	40.0	27.0	41.0
48	34.98939627643266	37.0	33.0	40.0	27.0	41.0
49	34.98091990132122	37.0	34.0	40.0	27.0	41.0
50	34.812799625159	36.0	33.0	40.0	27.0	41.0
51	33.716462029267156	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	11.0
11	13.0
12	16.0
13	23.0
14	33.0
15	48.0
16	90.0
17	173.0
18	324.0
19	604.0
20	1099.0
21	1897.0
22	2852.0
23	3768.0
24	4825.0
25	6578.0
26	8047.0
27	9730.0
28	12083.0
29	15215.0
30	19452.0
31	24483.0
32	30894.0
33	41269.0
34	55846.0
35	59227.0
36	72491.0
37	93126.0
38	112662.0
39	59120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.577080611254978	5.341484238281895	51.778686578522425	17.3027485719407
2	21.30697496709921	6.120725845632804	52.29204893687608	20.2802502503919
3	22.95649548822883	6.1694677540829215	49.35023891396739	21.523797843720864
4	20.27458989973318	5.41113799777674	47.50920986221763	26.805062240272452
5	20.48999768869015	5.994940275438952	47.87241569615237	25.64264633971852
6	22.475051218311865	8.759235412411577	50.90070329856935	17.865010070707214
7	84.0137546521007	2.8218420353363114	9.34240876222282	3.8219945503401713
8	85.17585608872913	5.012397740262232	5.677488942662221	4.134257228346407
9	79.88106974338172	5.617269100931914	7.3042108291942025	7.19745032649217
10	41.18015166595126	28.0203080803078	15.638448246313303	15.161092007427637
11	31.85157931644976	25.758997992147837	22.756024735732378	19.63339795567002
12	29.057881802444328	21.67379713617703	27.866063524857587	21.402257536521056
13	26.285725067334585	23.508536909417092	29.064013848991276	21.14172417425704
14	21.991562932879248	26.05459408210339	29.46071009099014	22.49313289402723
15	19.876321338106894	24.45743180456696	32.481765023120964	23.184481834205183
16	23.147689554923485	23.409166308963954	30.159134469491494	23.28400966662107
17	23.129765111170858	24.9045995066061	28.20992982737503	23.75570555484801
18	23.671586454780876	23.612310004827023	28.14687981031536	24.569223730076743
19	24.889819702108323	25.213245849469264	26.826131323279924	23.07080312514249
20	28.697663375801685	25.17613910626208	26.88933857230233	19.2368589456339
21	27.179274311599162	26.441856406337706	26.20931033344182	20.16955894862131
22	25.340760971253278	23.1299223431336	28.1819425380069	23.347374147606224
23	25.354125688086377	25.38510038474661	27.622196750644257	21.63857717652275
24	24.634946690503032	24.719380254495654	27.720938423246434	22.92473463175488
25	24.091081331377367	26.62141530778943	25.355226311825575	23.93227704900763
26	23.29690268756594	26.001135214770997	26.65128938071047	24.050672716952594
27	23.81010781395685	25.792173936286467	27.36622311529977	23.031495134456915
28	21.6866901571219	27.524241237855797	28.171407996503163	22.61766060851914
29	23.618756515299456	25.44138942740836	27.631001740557824	23.308852316734356
30	23.206179845063623	26.740282671622616	28.057572055477724	21.99596542783603
31	24.993750029480992	25.74909237849507	25.594061663231148	23.66309592879279
32	25.81277132340571	27.067954081977604	25.070636459261987	22.048638135354707
33	24.966234436001088	26.801917601017607	25.10066776414577	23.13118019883554
34	23.150205266327358	25.663243726837763	27.992792486827895	23.193758520006984
35	23.466555975364898	26.477548061880213	26.486981979644753	23.568913983110143
36	23.952874436126873	29.10269291182589	24.702084738594	22.24234791345324
37	23.444857964506458	28.442475900270907	25.88242508290055	22.230241052322082
38	23.374103581272415	27.535404707210496	25.271107211758437	23.81938449975865
39	23.61435402034267	26.4604097779413	25.9643429354893	23.960893266226734
40	23.98070449353226	25.20837165862425	27.903170268064777	22.90775357977871
41	20.820499274374495	25.76198539943994	28.22926935879233	25.188245967393236
42	22.575679674466944	25.395477694287607	26.83635140085817	25.192491230387276
43	23.056180552607454	26.671729535866966	26.611195230211177	23.660894681314396
44	23.571901390402246	25.72252017679162	27.280374463642467	23.425203969163668
45	24.027716850392213	24.828813700564307	25.309786274593044	25.833683174450435
46	23.114670842747596	26.644528406312553	26.443585957927873	23.797214793011985
47	21.87206664119509	26.964967146381387	28.524236520896913	22.638729691526613
48	22.023481021315938	25.951449914544426	28.237759884780417	23.787309179359216
49	23.329135239928114	25.703180645374314	27.538392114502603	23.42929200019497
50	21.713576822750834	26.175505461452225	27.50757464980511	24.60334306599183
51	21.261377697903942	25.467961629111812	27.208991152557456	26.06166952042679
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	118.0
1	439.0
2	760.0
3	1143.5
4	1527.0
5	1202.0
6	877.0
7	905.5
8	934.0
9	933.5
10	933.0
11	949.0
12	965.0
13	964.0
14	963.0
15	1067.5
16	1172.0
17	1188.5
18	1205.0
19	1235.5
20	1266.0
21	1451.0
22	1636.0
23	2163.5
24	2691.0
25	3230.5
26	5169.0
27	6568.0
28	7243.5
29	7919.0
30	9715.0
31	11511.0
32	12842.5
33	14174.0
34	16603.0
35	19032.0
36	19275.5
37	19519.0
38	21689.5
39	23860.0
40	26599.5
41	29339.0
42	31852.5
43	34366.0
44	35439.0
45	36512.0
46	42449.5
47	48387.0
48	49007.0
49	49627.0
50	48991.5
51	48356.0
52	46071.0
53	43786.0
54	41715.0
55	39644.0
56	39042.5
57	38441.0
58	36869.0
59	35297.0
60	34080.5
61	32864.0
62	28262.0
63	23660.0
64	21349.5
65	19039.0
66	15534.5
67	12030.0
68	10021.5
69	8013.0
70	7005.0
71	5997.0
72	5114.5
73	4232.0
74	3403.5
75	2012.5
76	1450.0
77	992.0
78	534.0
79	424.5
80	315.0
81	208.0
82	101.0
83	61.5
84	22.0
85	16.5
86	11.0
87	8.0
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	636003.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.302933803230964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.58911425112302	21.85679877342289
2	7.80739220386303	4.575589938513198
3	2.7146350738581635	2.386403156075843
4	1.4538455763510196	1.704077627357364
5	1.0129375901894186	1.4841021576062412
6	0.8281379728803754	1.4560123319553313
7	0.7433112469749998	1.524684018571384
8	0.6695272178871422	1.569528939616666
9	0.6072978451261696	1.6016047699119265
>10	9.392183312876874	55.1843221617067
>50	0.1337663364729801	2.6109333186976973
>100	0.041869950847231906	2.194898478277776
>500	0.003806359167930173	0.8411520193704811
>1k	0.0021750623816743845	1.0098923089164822
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC	2022	0.31792302866495914	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG	1777	0.2794011977930922	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTC	1331	0.2092757424100201	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1208	0.18993621099271543	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGATCAT	910	0.14308108609550585	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	895	0.1407226066543711	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	824	0.12955913729966684	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	753	0.11839566794496252	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	735	0.11556549261560088	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0935530178316769	0.0
2	0.0	0.0	0.0	0.4671361613074152	0.0
3	0.0	0.0	0.0	0.6176071496518099	0.0
4	0.0	0.0	0.0	0.8770398881766281	0.0
5	0.0	0.0	0.0	1.4550245832173747	0.0
6	0.0	0.0	0.0	1.6977907336915077	0.0
7	0.0	0.0	0.0	1.9562800804398721	0.0
8	0.0	0.0	0.0	2.331121079617549	0.0
9	0.0	0.0	0.0	2.476246181228705	0.0
10	0.0	0.0	0.0	3.050771773089121	0.0
11	0.0	0.0	0.0	3.637246994117952	0.0
12	0.0	0.0	0.0	4.544475419141104	0.0
13	0.0	0.0	0.0	4.752336073886444	0.0
14	0.0	0.0	0.0	4.836455173953582	0.0
15	0.0	0.0	0.0	5.015070683628851	0.0
16	0.0	0.0	0.0	5.306735974515844	0.0
17	0.0	0.0	0.0	5.679061262289643	0.0
18	0.0	0.0	0.0	6.0746568805493055	0.0
19	0.0	0.0	0.0	6.5301578766137895	0.0
20	0.0	0.0	0.0	6.871508467727353	0.0
21	0.0	0.0	0.0	7.315217066586164	0.0
22	0.0	0.0	0.0	7.811755604926391	0.0
23	0.0	0.0	0.0	8.274174807351537	0.0
24	0.0	0.0	0.0	8.665525162617158	0.0
25	0.0	0.0	0.0	9.032819027583203	0.0
26	1.5723196274231412E-4	0.0	0.0	9.347754648956059	0.0
27	1.5723196274231412E-4	0.0	0.0	9.686117832777517	0.0
28	1.5723196274231412E-4	0.0	0.0	10.042405460351603	0.0
29	1.5723196274231412E-4	0.0	0.0	10.39287550530422	0.0
30	1.5723196274231412E-4	0.0	0.0	10.806395567316507	0.0
31	1.5723196274231412E-4	0.0	0.0	11.176991303500142	0.0
32	1.5723196274231412E-4	0.0	0.0	11.574316473349969	0.0
33	1.5723196274231412E-4	0.0	0.0	11.955289519074595	0.0
34	1.5723196274231412E-4	0.0	0.0	12.318180889083857	0.0
35	1.5723196274231412E-4	0.0	0.0	12.757172529060398	0.0
36	1.5723196274231412E-4	0.0	0.0	13.162202065084598	0.0
37	1.5723196274231412E-4	0.0	0.0	13.565187585593149	0.0
38	1.5723196274231412E-4	0.0	0.0	13.994745307805152	0.0
39	1.5723196274231412E-4	0.0	0.0	14.497573124655073	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	20	7.030908E-4	45.0	39
GCCGATA	20	7.030908E-4	45.0	9
ACGCGCC	20	7.030908E-4	45.0	40
CGTTCAT	140	0.0	41.785713	17
TCGTTTG	245	0.0	41.32653	1
TTTCGCG	210	0.0	40.714287	1
CGGGCGA	260	0.0	40.673077	6
TTTGTCG	205	0.0	40.609756	1
TCGTTGA	50	1.0804797E-9	40.500004	24
TTAATCG	50	1.0804797E-9	40.500004	20
GGCCGAT	250	0.0	40.500004	8
CGATGAA	615	0.0	40.2439	19
CCGATGA	620	0.0	39.55645	18
TGTTTCG	165	0.0	39.545452	1
TTGGGAC	1190	0.0	39.516808	5
CGGTAGT	115	0.0	39.130436	12
TCGTTCA	150	0.0	39.0	16
TGGGACC	660	0.0	38.863636	6
TTGGGCA	915	0.0	38.60656	5
TCGAATG	70	0.0	38.571426	44
>>END_MODULE