FastQCFastQC Report
Sat 18 Jun 2016
SRR3549642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432229
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC14790.3421797241739911No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT9700.22441807467800634No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGC7500.173519129905675No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC7460.17259369454617807No Hit
TCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC7340.16981738846768726No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCG6180.1429797630422762No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.1228515439732179No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTC4410.1020292483845369No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGG207.028395E-445.0000044
GCGACTC207.028395E-445.00000431
GACCGGA207.028395E-445.00000428
ATCGTCG207.028395E-445.00000421
CATACTC207.028395E-445.00000430
GCGATGA207.028395E-445.0000049
GCGATAT207.028395E-445.0000049
TACCGAA207.028395E-445.00000430
CGAACGA253.886753E-545.022
TCTAGCG253.886753E-545.01
CGCTAGA253.886753E-545.024
GTCGTTT253.886753E-545.09
CGGGACG351.2095734E-745.06
CTAGCGG551.8189894E-1245.02
TAGCGGG1500.040.53
TCGTCTT501.0786607E-940.538
GGCGATA451.9235813E-840.08
TGTTTCG1250.039.61
CGTTTGG2850.039.4736822
TTTAGCG403.4515506E-739.3750041