##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549639_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	420416
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.98236270741361	31.0	30.0	31.0	26.0	34.0
2	30.025795878368093	31.0	30.0	33.0	25.0	34.0
3	30.085139480895112	31.0	30.0	34.0	25.0	34.0
4	34.00508306058761	35.0	33.0	37.0	28.0	37.0
5	34.50386521921145	35.0	35.0	37.0	32.0	37.0
6	33.81455510732227	35.0	35.0	37.0	29.0	37.0
7	34.4764994671944	35.0	35.0	37.0	32.0	37.0
8	34.28423038133658	35.0	35.0	37.0	31.0	37.0
9	35.83834582889329	37.0	35.0	39.0	31.0	39.0
10	35.3223569036383	37.0	34.0	39.0	30.0	39.0
11	35.538614134571475	37.0	35.0	39.0	30.0	39.0
12	35.57907405997869	37.0	35.0	39.0	30.0	39.0
13	35.521193294260925	37.0	35.0	39.0	30.0	39.0
14	36.250749257877914	38.0	35.0	40.0	30.0	41.0
15	36.47688479981733	38.0	35.0	40.0	30.0	41.0
16	36.703912315420915	38.0	35.0	40.0	31.0	41.0
17	36.42754557390775	38.0	35.0	40.0	30.0	41.0
18	36.56307562033795	38.0	35.0	40.0	31.0	41.0
19	36.55344944055412	38.0	35.0	40.0	31.0	41.0
20	36.52042262901507	38.0	35.0	40.0	30.0	41.0
21	36.30060701781093	38.0	35.0	40.0	30.0	41.0
22	36.45907862688385	38.0	35.0	40.0	30.0	41.0
23	36.5293566372355	38.0	35.0	40.0	30.0	41.0
24	36.416071224691734	38.0	35.0	40.0	30.0	41.0
25	36.004388510427766	38.0	34.0	40.0	30.0	41.0
26	36.114698298827825	38.0	34.0	40.0	30.0	41.0
27	36.08145503501294	38.0	34.0	40.0	30.0	41.0
28	35.97920868853707	38.0	34.0	40.0	30.0	41.0
29	36.16415883315573	38.0	35.0	40.0	30.0	41.0
30	35.66426586999543	38.0	34.0	40.0	29.0	41.0
31	35.8395731846552	38.0	34.0	40.0	29.0	41.0
32	35.89102698279799	38.0	34.0	40.0	30.0	41.0
33	35.8710872088598	38.0	34.0	40.0	29.0	41.0
34	35.91769580605876	38.0	34.0	40.0	30.0	41.0
35	35.94106789465672	38.0	34.0	40.0	30.0	41.0
36	35.69706195768001	38.0	34.0	40.0	29.0	41.0
37	35.72923961029076	38.0	34.0	40.0	29.0	41.0
38	35.6023866836657	38.0	34.0	40.0	29.0	41.0
39	35.623991475110365	38.0	34.0	40.0	29.0	41.0
40	35.575518058304155	38.0	34.0	40.0	29.0	41.0
41	35.51451419546354	38.0	34.0	40.0	28.0	41.0
42	35.77034413533262	38.0	34.0	40.0	29.0	41.0
43	35.73203683970163	38.0	34.0	40.0	29.0	41.0
44	35.70232817019333	38.0	34.0	40.0	29.0	41.0
45	35.792595905008376	38.0	34.0	40.0	29.0	41.0
46	35.68041653980819	38.0	34.0	40.0	29.0	41.0
47	35.567359472522455	38.0	34.0	40.0	29.0	41.0
48	35.591866151621254	38.0	34.0	40.0	29.0	41.0
49	35.674655579235804	38.0	34.0	40.0	29.0	41.0
50	35.512896749885826	38.0	34.0	40.0	29.0	41.0
51	34.46687804460344	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	4.0
12	3.0
13	2.0
14	13.0
15	11.0
16	36.0
17	57.0
18	121.0
19	277.0
20	511.0
21	853.0
22	1301.0
23	1955.0
24	2417.0
25	3082.0
26	3961.0
27	5408.0
28	6919.0
29	9392.0
30	12185.0
31	16200.0
32	20957.0
33	28238.0
34	37889.0
35	41176.0
36	50754.0
37	64698.0
38	76364.0
39	35624.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.722103820977317	4.410393514994672	52.73752664028011	16.129976023747908
2	19.53826685949155	8.533928299588979	51.84769371289389	20.080111128025575
3	20.484948241741513	8.39977546049627	50.39270627188309	20.72257002587913
4	19.99257877911402	4.495071548180849	50.08467803318618	25.42767163951895
5	20.946395950677424	5.297134267011722	48.52431876998021	25.232151012330643
6	21.07674303546963	9.896626198812605	51.140536991931796	17.886093773785962
7	79.09737022377836	2.5353459430659155	13.631498325468108	4.735785507687624
8	79.54097845943066	6.4864324859187095	7.904789541787183	6.067799512863449
9	73.39611242198204	5.628948470086772	10.479144466433247	10.495794641497945
10	38.76802976099863	24.079721038209772	20.237336352565077	16.914912848226518
11	30.290712056629626	26.158138605571622	24.77855267163952	18.772596666159235
12	25.446700411021467	22.376170269447403	30.14419051606028	22.03293880347085
13	23.739581747602376	24.362536154665854	32.89836733140508	18.999514766326687
14	19.854144466433247	28.141412315420915	30.030969325620337	21.9734738925255
15	16.894219059217537	26.057523976252096	36.36160374486223	20.686653219668138
16	18.46028695387426	24.611575201704976	32.145065839549396	24.783072004871364
17	19.338464758715176	26.095343659613334	30.539275384381188	24.0269161972903
18	19.745442609225147	24.35325962855838	32.61483863601766	23.286459126198814
19	21.349330187243112	26.420497792662506	29.274337798751716	22.95583422134267
20	23.793575886740754	26.118891764347694	31.737850129395646	18.34968221951591
21	23.727688765413305	28.41328588826305	29.077627873344497	18.781397472979144
22	20.29561196529152	25.280198660374488	30.344706195768	24.07948317856599
23	20.71805069264728	27.214235423961032	28.311481960724617	23.756231922667073
24	21.17402762977622	25.268305678185417	29.648728878063636	23.908937813974728
25	18.898424417719593	29.106171030598265	27.96468260009134	24.030721951590806
26	18.794004034099558	27.48325468107779	30.297372126655503	23.425369158167147
27	19.783738011873954	29.928451819150553	28.707993986908203	21.579816182067287
28	17.108530598264576	28.684208022530065	32.7268705282387	21.48039085096666
29	20.38219287562795	25.20218069721419	31.090634038666465	23.3249923884914
30	18.853231085401127	29.869462627492773	29.93201971380728	21.34528657329883
31	20.510637083269902	28.843573983863603	26.335581899832544	24.310207033033947
32	21.712066144009743	28.84642829958898	27.153581214796773	22.287924341604505
33	19.59749391079312	27.241827142639668	28.599767848987668	24.56091109757954
34	19.28756279494596	25.702399528086467	29.84948241741513	25.160555259552442
35	19.0632611508601	24.965034632364137	29.308589587456236	26.66311462931953
36	19.342508372659463	28.112155579235804	30.715529380423202	21.829806667681535
37	19.250218830872278	29.15445653828589	29.986727431876997	21.608597198964834
38	19.40054612574212	28.613087989039425	28.145931648652763	23.840434236565688
39	21.19900289237327	25.174588978535546	30.767382782767545	22.85902534632364
40	19.397216090729184	23.510047191353326	31.682190972750803	25.41054574516669
41	17.303575506165323	24.864895722332168	29.611860633277516	28.219668138224996
42	18.702665930887502	24.59992007915969	30.522863068960266	26.17455092099254
43	19.867464606485004	25.25379623991475	31.129880879890393	23.74885827370985
44	19.44288514233521	25.576096057238544	30.05689602679251	24.924122773633734
45	19.721418785203227	24.949573755518344	28.177091261988124	27.151916197290305
46	21.894980210077637	25.969278048409194	29.56690516060283	22.568836580910336
47	16.88280179631603	25.688841528390927	33.60148043842289	23.82687623687015
48	18.314716851880043	24.86085210838788	31.003101689754907	25.82132934997717
49	19.453588826305374	22.44824174151317	33.769647206576344	24.328522225605116
50	20.292995509209927	22.63591300045669	30.947442533110063	26.123648957223324
51	18.447918252397624	22.628301491855684	28.62165093621556	30.30212931953113
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102.0
1	225.0
2	348.0
3	584.5
4	821.0
5	637.5
6	454.0
7	525.0
8	596.0
9	657.0
10	718.0
11	787.0
12	856.0
13	899.0
14	942.0
15	961.0
16	980.0
17	979.0
18	978.0
19	1047.5
20	1117.0
21	1284.0
22	1451.0
23	1695.5
24	1940.0
25	2367.0
26	3609.0
27	4424.0
28	5394.5
29	6365.0
30	7097.5
31	7830.0
32	8898.5
33	9967.0
34	11329.5
35	12692.0
36	14389.0
37	16086.0
38	17524.5
39	18963.0
40	21628.0
41	24293.0
42	27488.0
43	30683.0
44	41359.0
45	52035.0
46	51161.5
47	50288.0
48	47306.0
49	44324.0
50	37416.5
51	30509.0
52	26192.0
53	21875.0
54	19372.5
55	16870.0
56	15743.5
57	14617.0
58	13403.0
59	12189.0
60	10941.5
61	9694.0
62	8531.0
63	7368.0
64	6267.5
65	5167.0
66	4384.5
67	3602.0
68	2930.5
69	2259.0
70	1986.0
71	1713.0
72	1415.5
73	1118.0
74	940.0
75	566.0
76	370.0
77	266.0
78	162.0
79	115.0
80	68.0
81	42.0
82	16.0
83	11.0
84	6.0
85	5.0
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	420416.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.27702481683339
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.50731439180122	27.02856957985115
2	10.910777751355907	8.134426660164957
3	4.031327400101473	4.508276746150892
4	2.1307723521049113	3.17715415393749
5	1.3386432556772818	2.4950318931384343
6	1.010334377588665	2.259735580000356
7	0.8702044177927357	2.2707042174444507
8	0.7629830171672385	2.2753389492612457
9	0.678928983536215	2.2777607311332266
>10	5.630908785727211	35.54508209499716
>50	0.0826215868425839	2.152071001517087
>100	0.03743790653804583	2.6325814878799703
>500	0.002581924588830747	0.6720409556392246
>1k	0.005163849177661494	4.57122594888435
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGC	3979	0.9464435226061805	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCG	3548	0.8439260161363982	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	2480	0.5898919165778658	No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	2151	0.511636093773786	No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	2049	0.4873744101080834	No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	1699	0.4041235347845943	No Hit
TCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	1614	0.3839054650631755	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAAGTCCTT	1478	0.35155655350890547	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTA	782	0.18600624143705283	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTC	748	0.1779190135484853	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCT	653	0.15532234738925255	No Hit
GAACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT	610	0.14509438270665245	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCC	444	0.10560968183894047	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.43861318313289693	0.0
2	0.0	0.0	0.0	1.9901716395189526	0.0
3	0.0	0.0	0.0	2.5320159080529763	0.0
4	0.0	0.0	0.0	3.492493149642259	0.0
5	0.0	0.0	0.0	5.44841300045669	0.0
6	0.0	0.0	0.0	6.209801720200944	0.0
7	0.0	0.0	0.0	7.038742578779114	0.0
8	0.0	0.0	0.0	8.27894276145532	0.0
9	0.0	0.0	0.0	8.74514766326686	0.0
10	0.0	0.0	0.0	10.627331024509058	0.0
11	0.0	0.0	0.0	12.402239686405846	0.0
12	0.0	0.0	0.0	15.10408738011874	0.0
13	0.0	0.0	0.0	15.726090348607094	0.0
14	0.0	0.0	0.0	15.976080834221342	0.0
15	0.0	0.0	0.0	16.510551453798143	0.0
16	0.0	0.0	0.0	17.34615238240219	0.0
17	0.0	0.0	0.0	18.444112498097123	0.0
18	0.0	0.0	0.0	19.65600738316334	0.0
19	0.0	0.0	0.0	20.854106408890242	0.0
20	0.0	0.0	0.0	21.674484320292283	0.0
21	0.0	0.0	0.0	22.776488049931498	0.0
22	0.0	0.0	0.0	23.895379814279192	0.0
23	0.0	0.0	0.0	24.940535089054652	0.0
24	0.0	0.0	0.0	25.780417491246766	0.0
25	0.0	0.0	0.0	26.55203417567362	0.0
26	0.0	0.0	0.0	27.254909423047646	0.0
27	0.0	0.0	0.0	27.931857969249506	0.0
28	0.0	0.0	0.0	28.6352089359111	0.0
29	0.0	0.0	0.0	29.353307200487137	0.0
30	0.0	0.0	0.0	30.14847198964835	0.0
31	0.0	0.0	0.0	30.913666463693104	0.0
32	0.0	0.0	0.0	31.65031778048409	0.0
33	0.0	0.0	0.0	32.3470086771198	0.0
34	0.0	0.0	0.0	33.04607817019333	0.0
35	0.0	0.0	0.0	33.7791615923276	0.0
36	0.0	0.0	0.0	34.48893476937129	0.0
37	0.0	0.0	0.0	35.19989724463389	0.0
38	0.0	0.0	0.0	35.92156340386665	0.0
39	0.0	0.0	0.0	36.61397282691429	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCG	20	7.028176E-4	45.000004	17
TTAGGCG	20	7.028176E-4	45.000004	1
GCACGAA	20	7.028176E-4	45.000004	34
CGATTAT	20	7.028176E-4	45.000004	10
TCGATGG	20	7.028176E-4	45.000004	2
TCGGCAT	20	7.028176E-4	45.000004	21
TCCGTTG	20	7.028176E-4	45.000004	1
ACGTCAG	20	7.028176E-4	45.000004	12
GCTCGCG	20	7.028176E-4	45.000004	1
GCAAACG	25	3.8865714E-5	45.0	10
GTTCGTA	25	3.8865714E-5	45.0	32
TGCGTCG	25	3.8865714E-5	45.0	1
TGTTGCG	60	0.0	44.999996	1
TGCACGG	30	2.1622054E-6	44.999996	2
CGATGAA	255	0.0	43.235294	19
CTTTGCG	170	0.0	42.35294	1
GCGCGAC	70	0.0	41.785713	9
TCAGACG	70	0.0	41.785713	22
TTTCGCG	135	0.0	41.666668	1
CGACCCG	65	0.0	41.538464	32
>>END_MODULE