FastQCFastQC Report
Sat 18 Jun 2016
SRR3549638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745716
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC9370.12565105214317515TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC8880.11908018602256086TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT8290.11116832681610693TruSeq Adapter, Index 16 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8000.10727944686717195No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG207.0316903E-445.00000435
GGACGCA207.0316903E-445.0000048
TAACGAA253.889483E-545.019
CGATAAC253.889483E-545.016
TTTAGCG650.041.538461
TTTGTCG2350.040.2127651
TATGGGC1750.039.8571434
TGGGCGA9700.039.432996
GTCCGTA800.039.37500420
TTTGGGC14950.039.1304364
CATACGA1650.038.18181618
TTTGGGA34300.037.5874634
CTCCGTT301.1395373E-437.50000415
TTTCGCG2400.037.5000041
TACGGGA601.546141E-1037.5000044
CCGTAAT301.1395373E-437.50000435
CGCTCGT301.1395373E-437.50000418
TTGGGAC12300.037.3170745
TTTTGCG6400.037.265631
TGTTGCG1700.037.0588231
Produced by FastQC (version 0.11.5)