##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549638_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	745716
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.134049691839788	31.0	30.0	31.0	26.0	34.0
2	30.520948993986988	31.0	30.0	33.0	27.0	34.0
3	30.542702583825477	31.0	30.0	34.0	27.0	34.0
4	34.084559001013794	35.0	33.0	37.0	30.0	37.0
5	34.465493565915175	35.0	35.0	37.0	32.0	37.0
6	33.98930021616808	35.0	35.0	37.0	30.0	37.0
7	34.36792827296183	35.0	35.0	37.0	32.0	37.0
8	34.63448819657886	35.0	35.0	37.0	32.0	37.0
9	35.94096277939591	37.0	35.0	39.0	31.0	39.0
10	35.3100496703839	37.0	34.0	39.0	30.0	39.0
11	35.459229787211214	37.0	35.0	39.0	30.0	39.0
12	35.48931228510586	37.0	35.0	39.0	30.0	39.0
13	35.521498801152184	37.0	35.0	39.0	30.0	39.0
14	36.275399750038886	38.0	35.0	40.0	30.0	41.0
15	36.55391730900236	38.0	35.0	40.0	31.0	41.0
16	36.67649212300661	38.0	35.0	40.0	31.0	41.0
17	36.45227271508188	38.0	35.0	40.0	30.0	41.0
18	36.5423123548375	38.0	35.0	40.0	31.0	41.0
19	36.55013034452794	38.0	35.0	40.0	30.0	41.0
20	36.60252428538478	38.0	35.0	40.0	31.0	41.0
21	36.27214650081264	38.0	35.0	40.0	30.0	41.0
22	36.448090157647144	38.0	35.0	40.0	30.0	41.0
23	36.53599761839628	38.0	35.0	40.0	30.0	41.0
24	36.43188827918403	38.0	35.0	40.0	30.0	41.0
25	35.91875727488749	38.0	34.0	40.0	29.0	41.0
26	36.0457788219644	38.0	34.0	40.0	30.0	41.0
27	36.106791325383924	38.0	34.0	40.0	30.0	41.0
28	35.99854770448803	38.0	34.0	40.0	30.0	41.0
29	36.11709953923478	38.0	35.0	40.0	30.0	41.0
30	35.65487799644905	38.0	34.0	40.0	29.0	41.0
31	35.83164636403135	38.0	34.0	40.0	29.0	41.0
32	35.91707835154402	38.0	34.0	40.0	30.0	41.0
33	35.95176045572309	38.0	34.0	40.0	30.0	41.0
34	35.924008603811636	38.0	34.0	40.0	29.0	41.0
35	35.94917636204668	38.0	34.0	40.0	30.0	41.0
36	35.74039044354687	38.0	34.0	40.0	29.0	41.0
37	35.81535061605222	38.0	34.0	40.0	29.0	41.0
38	35.72306481287782	38.0	34.0	40.0	29.0	41.0
39	35.63755236578	38.0	34.0	40.0	29.0	41.0
40	35.53622558722087	38.0	34.0	40.0	28.0	41.0
41	35.433218544325186	38.0	34.0	40.0	28.0	41.0
42	35.64846000354022	38.0	34.0	40.0	29.0	41.0
43	35.61011832922989	38.0	34.0	40.0	29.0	41.0
44	35.55606557992587	38.0	34.0	40.0	29.0	41.0
45	35.61083441953773	38.0	34.0	40.0	29.0	41.0
46	35.50798159084692	37.0	34.0	40.0	29.0	41.0
47	35.481067859614114	37.0	34.0	40.0	29.0	41.0
48	35.441725804461754	37.0	34.0	40.0	29.0	41.0
49	35.43245820124552	37.0	34.0	40.0	29.0	41.0
50	35.271732133949115	37.0	34.0	40.0	28.0	41.0
51	34.16076093311663	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	9.0
11	11.0
12	16.0
13	27.0
14	28.0
15	70.0
16	101.0
17	183.0
18	324.0
19	579.0
20	933.0
21	1539.0
22	2266.0
23	3269.0
24	4484.0
25	6101.0
26	8382.0
27	10336.0
28	13022.0
29	17253.0
30	21775.0
31	27992.0
32	35745.0
33	47754.0
34	66617.0
35	69984.0
36	85982.0
37	112173.0
38	137787.0
39	70961.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.905347344029092	4.759586759570668	53.862730583761106	17.472335312639128
2	19.962425373734774	3.8308149483181264	55.095773726190664	21.110985951756433
3	22.801710034383063	3.9456039564660004	51.5563565754255	21.696329433725438
4	20.149225710592237	5.420562251581031	46.76874306036078	27.66146897746595
5	18.339019144017293	5.943951852984246	49.96392728599091	25.75310171700755
6	22.654871291483623	6.8444287101255705	52.176029480391996	18.324670517998808
7	86.44658288141866	2.6409517832526057	7.018891910593308	3.893573424735422
8	88.28562079933916	2.8787098573719754	5.114949927318175	3.7207194159706916
9	82.62059550820956	5.390926304383974	7.065156172054776	4.923322015351689
10	42.015056670367805	26.97219853134437	15.588642324960173	15.424102473327647
11	31.1534149729924	27.41311705796845	22.309163273954162	19.124304695084994
12	28.505087727767673	23.20776274077531	27.958096648053683	20.329052883403335
13	25.704021370065817	24.439062592193274	28.996293495110738	20.860622542630168
14	21.561693727907137	25.96283303563287	29.39309334921069	23.082379887249303
15	19.80566328200012	26.498827972042978	32.66726206759678	21.028246678360127
16	23.10464037247424	24.180921423169142	31.349334062833574	21.365104141523048
17	23.332475097758394	24.651878194916026	28.556715961572504	23.458930745753076
18	23.13213073073395	24.888965772492476	29.35554554280718	22.623357953966387
19	25.94204764280235	26.0227754265699	26.906892168063983	21.128284762563766
20	27.88393973040675	26.0545569627043	27.01886509073159	19.042638216157357
21	27.025704155469377	26.05656845233306	26.69970873630176	20.21801865589581
22	25.465592799403524	24.258564922839255	27.371278073690252	22.90456420406696
23	24.49565250041571	25.682565480692382	27.35196777325416	22.46981424563775
24	23.74617146473993	25.548332072799834	28.312118822715348	22.39337763974489
25	23.34615322723396	26.920436198230963	26.210246259970283	23.52316431456479
26	22.92199711418288	28.26880474604273	25.963369432867207	22.845828706907188
27	22.742974537223287	27.25246608628486	27.739112477136068	22.265446899355787
28	21.016982336439074	27.407216688390758	28.662252117428082	22.91354885774209
29	22.733453486313824	26.307334159385075	27.958498945979432	23.000713408321666
30	23.34508043276529	26.65076248866861	27.35223597187133	22.651921106694775
31	23.268107429638093	27.43470704665047	25.90382933985592	23.393356183855516
32	24.327089669525666	27.284784019653596	25.91093660321087	22.477189707609867
33	24.42444576755762	26.87175814921498	25.951836892328984	22.75195919089841
34	22.049815211152772	26.826164384296437	27.449055672668955	23.674964731881843
35	22.185926009365495	26.34903904435469	27.783365248968778	23.68166969731104
36	22.9268246892919	27.987598495942155	26.46785103176008	22.617725783005863
37	22.445274072166885	28.06966727279554	26.654383170000372	22.8306754850372
38	22.252573365731728	26.885302179381966	26.680130237248495	24.181994217637815
39	22.10855070831255	26.547774219676125	27.277140359064312	24.06653471294702
40	22.976575532776554	26.40670174704579	28.077310933384826	22.539411786792826
41	20.151237200220994	26.481931459161395	28.791389751594444	24.575441589023168
42	21.95768898615559	26.289364852034826	27.62620085930837	24.12674530250122
43	21.90632895096793	26.463023456651058	27.52294439169872	24.107703200682298
44	21.953666006898068	26.073867263140393	27.403864205676154	24.568602524285385
45	21.95299551035515	26.29848360501853	26.2385412140815	25.50997967054482
46	21.650199271572557	27.05078072617458	26.804172097688667	24.494847904564203
47	20.812480890848526	27.08095307060597	28.357042091090978	23.749523947454527
48	20.966292797794335	24.996781616593985	29.34642678982347	24.69049879578821
49	21.279414683337894	25.64448127705454	28.78025950898197	24.295844530625597
50	20.82991380096444	25.997698855864698	27.792752200569655	25.379635142601202
51	19.717157738334702	26.076012852077735	27.580070697155485	26.626758712432082
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	94.0
1	387.5
2	681.0
3	1028.5
4	1376.0
5	1093.5
6	811.0
7	801.0
8	791.0
9	930.0
10	1069.0
11	1062.5
12	1056.0
13	1080.0
14	1104.0
15	1126.0
16	1148.0
17	1096.0
18	1044.0
19	1223.5
20	1403.0
21	1643.5
22	1884.0
23	2721.0
24	3558.0
25	3965.5
26	5519.5
27	6666.0
28	8291.5
29	9917.0
30	12238.5
31	14560.0
32	16644.5
33	18729.0
34	21272.0
35	23815.0
36	25930.5
37	28046.0
38	29478.0
39	30910.0
40	34355.5
41	37801.0
42	40727.5
43	43654.0
44	46475.0
45	49296.0
46	52497.5
47	55699.0
48	58247.0
49	60795.0
50	60625.5
51	60456.0
52	55764.0
53	51072.0
54	47258.5
55	43445.0
56	41830.5
57	40216.0
58	38887.5
59	37559.0
60	35190.0
61	32821.0
62	29430.5
63	26040.0
64	22230.0
65	18420.0
66	15431.0
67	12442.0
68	10434.0
69	8426.0
70	7089.5
71	5753.0
72	4857.0
73	3961.0
74	3238.0
75	1806.5
76	1098.0
77	828.0
78	558.0
79	467.0
80	376.0
81	263.0
82	150.0
83	114.5
84	79.0
85	60.5
86	42.0
87	23.5
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	745716.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.90376414941862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.91228177092954	22.102554418232504
2	8.818326162652559	5.274022915212197
3	2.9451893565154106	2.642167436778445
4	1.5368097585504528	1.8382558624887086
5	1.0249342992924246	1.5324696777345153
6	0.8013682196051306	1.4378355741546804
7	0.6923351557980202	1.4492399047934497
8	0.617481921468551	1.4772026996900305
9	0.5874678183831399	1.5810749177672618
>10	8.883381271952716	55.674736773353196
>50	0.14306368476137435	2.742916196852543
>100	0.034626879108429494	1.603894767778764
>500	0.0027337009822444337	0.6436288551636834
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	937	0.12565105214317515	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	888	0.11908018602256086	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	829	0.11116832681610693	TruSeq Adapter, Index 16 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	800	0.10727944686717195	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12149397357707224	0.0
2	0.0	0.0	0.0	0.5081022802246432	0.0
3	0.0	0.0	0.0	0.654136427272581	0.0
4	0.0	0.0	0.0	0.9323924925843082	0.0
5	0.0	0.0	0.0	1.3816251763405907	0.0
6	0.0	0.0	0.0	1.6628314264411652	0.0
7	0.0	0.0	0.0	1.9417579882958123	0.0
8	0.0	0.0	0.0	2.386163096943072	0.0
9	0.0	0.0	0.0	2.597101309345649	0.0
10	0.0	0.0	0.0	3.0386903325126458	0.0
11	0.0	0.0	0.0	3.8532095328516487	0.0
12	0.0	0.0	0.0	4.662901158081629	0.0
13	0.0	0.0	0.0	4.904145814224182	0.0
14	0.0	0.0	0.0	4.988762477940663	0.0
15	0.0	0.0	0.0	5.140294696640544	0.0
16	0.0	0.0	0.0	5.458646455218877	0.0
17	0.0	0.0	0.0	5.932151113828857	0.0
18	0.0	0.0	0.0	6.499122990521861	0.0
19	0.0	0.0	0.0	6.875137451791298	0.0
20	0.0	0.0	0.0	7.299561763459548	0.0
21	0.0	0.0	0.0	7.894962693572352	0.0
22	0.0	0.0	0.0	8.536896083763793	0.0
23	0.0	0.0	0.0	9.125726147755982	0.0
24	0.0	0.0	0.0	9.599364905674546	0.0
25	0.0	0.0	0.0	10.024057415959962	0.0
26	0.0	0.0	0.0	10.405838147498512	0.0
27	0.0	0.0	0.0	10.770185968921144	0.0
28	0.0	0.0	0.0	11.173422589833127	0.0
29	0.0	0.0	0.0	11.626275954921176	0.0
30	0.0	0.0	0.0	12.100987507308412	0.0
31	0.0	0.0	0.0	12.587365699542453	0.0
32	0.0	0.0	0.0	13.038073475693158	0.0
33	0.0	0.0	0.0	13.48864715253528	0.0
34	0.0	0.0	0.0	13.960944917368007	0.0
35	0.0	0.0	0.0	14.453759876414077	0.0
36	0.0	0.0	0.0	14.930482918430073	0.0
37	0.0	0.0	0.0	15.406669563211732	0.0
38	0.0	0.0	0.0	15.889963471348342	0.0
39	0.0	0.0	0.0	16.425556109832698	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	20	7.0316903E-4	45.000004	35
GGACGCA	20	7.0316903E-4	45.000004	8
TAACGAA	25	3.889483E-5	45.0	19
CGATAAC	25	3.889483E-5	45.0	16
TTTAGCG	65	0.0	41.53846	1
TTTGTCG	235	0.0	40.212765	1
TATGGGC	175	0.0	39.857143	4
TGGGCGA	970	0.0	39.43299	6
GTCCGTA	80	0.0	39.375004	20
TTTGGGC	1495	0.0	39.130436	4
CATACGA	165	0.0	38.181816	18
TTTGGGA	3430	0.0	37.587463	4
CTCCGTT	30	1.1395373E-4	37.500004	15
TTTCGCG	240	0.0	37.500004	1
TACGGGA	60	1.546141E-10	37.500004	4
CCGTAAT	30	1.1395373E-4	37.500004	35
CGCTCGT	30	1.1395373E-4	37.500004	18
TTGGGAC	1230	0.0	37.317074	5
TTTTGCG	640	0.0	37.26563	1
TGTTGCG	170	0.0	37.058823	1
>>END_MODULE