FastQCFastQC Report
Sat 18 Jun 2016
SRR3549637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285073
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGC41501.4557674700866094No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCG41091.441385189056838No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC27490.9643144036790576No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC18500.6489565830506572No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGGAATATT17170.6023018665394478No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC17090.5994955678019314No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT13930.4886467676700354No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC11820.4146306384680415No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGGAATATTCGTA8730.3062373497314723No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTC7720.27080782817032834No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCT7690.26975546614375967No Hit
GAACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT7210.25291767371866153No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGC3260.11435667355379148No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.10172832923496788No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC2860.10032517986620972No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCC207.024351E-445.00000430
GAGCTAG207.024351E-445.00000445
CCGATCG207.024351E-445.00000440
TTAGCGT207.024351E-445.00000428
CGGCATG207.024351E-445.0000045
TAGCGTC207.024351E-445.00000429
CGATTCG207.024351E-445.00000410
CCTCCGC207.024351E-445.00000445
TACGAGA207.024351E-445.00000435
ACGGATG207.024351E-445.00000420
CTGCCCG207.024351E-445.0000041
GACGGAT207.024351E-445.00000419
TCATCGA302.1597389E-645.00000416
CATCGAC302.1597389E-645.00000417
ACCAACG302.1597389E-645.00000436
TTCATCG302.1597389E-645.00000415
GTCCAAC207.024351E-445.0000048
CGATCGA207.024351E-445.00000441
CTTAGTT207.024351E-445.00000426
TCGACAA302.1597389E-645.00000419