##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549637_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285073
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.777393860519936	31.0	30.0	31.0	25.0	34.0
2	29.65261178715627	31.0	30.0	33.0	25.0	34.0
3	29.677261613691932	31.0	30.0	33.0	25.0	34.0
4	33.7800949230548	35.0	33.0	37.0	28.0	37.0
5	34.349773566770615	35.0	35.0	37.0	32.0	37.0
6	33.55568222876246	35.0	33.0	37.0	28.0	37.0
7	34.40056406604624	35.0	35.0	37.0	32.0	37.0
8	34.05329512089886	35.0	35.0	37.0	30.0	37.0
9	35.69528857520705	37.0	35.0	39.0	30.0	39.0
10	35.20258670586131	37.0	34.0	39.0	30.0	39.0
11	35.37175740950563	37.0	34.0	39.0	30.0	39.0
12	35.3859467574972	37.0	35.0	39.0	30.0	39.0
13	35.22735229221989	37.0	34.0	39.0	29.0	39.0
14	35.9222023832492	38.0	34.0	40.0	29.0	41.0
15	36.08632525703943	38.0	34.0	40.0	30.0	41.0
16	36.44337064541363	38.0	35.0	40.0	31.0	41.0
17	36.09629112543103	38.0	34.0	40.0	30.0	41.0
18	36.2608068810445	38.0	35.0	40.0	30.0	41.0
19	36.26441648279563	38.0	34.0	40.0	30.0	41.0
20	36.15446569825974	38.0	34.0	40.0	30.0	41.0
21	36.01071304543047	38.0	34.0	40.0	30.0	41.0
22	36.21581138866185	38.0	34.0	40.0	30.0	41.0
23	36.19760201772879	38.0	34.0	40.0	30.0	41.0
24	36.08831422126964	38.0	34.0	40.0	30.0	41.0
25	35.750951510665686	38.0	34.0	40.0	29.0	41.0
26	35.816008531148164	38.0	34.0	40.0	29.0	41.0
27	35.686413655449655	38.0	34.0	40.0	29.0	41.0
28	35.58587449530471	38.0	34.0	40.0	28.0	41.0
29	35.67237865388865	38.0	34.0	40.0	29.0	41.0
30	35.28187166094299	38.0	34.0	40.0	27.0	41.0
31	35.517456932084066	38.0	34.0	40.0	29.0	41.0
32	35.39754729490341	38.0	34.0	40.0	27.0	41.0
33	35.42426676675799	38.0	34.0	40.0	28.0	41.0
34	35.40191459731367	38.0	34.0	40.0	27.0	41.0
35	35.41201727276873	38.0	34.0	40.0	28.0	41.0
36	35.18522974816977	38.0	34.0	40.0	27.0	41.0
37	35.16870064860579	38.0	34.0	40.0	27.0	41.0
38	35.01974932736527	37.0	33.0	40.0	26.0	41.0
39	35.05735373044799	37.0	33.0	40.0	27.0	41.0
40	34.91780000210473	37.0	33.0	40.0	26.0	41.0
41	34.83752231884465	37.0	33.0	40.0	26.0	41.0
42	35.08606567440621	37.0	34.0	40.0	27.0	41.0
43	35.0805372658933	37.0	33.0	40.0	27.0	41.0
44	35.075093046342516	37.0	33.0	40.0	27.0	41.0
45	35.13508469760377	37.0	34.0	40.0	27.0	41.0
46	35.008874919757396	37.0	33.0	40.0	27.0	41.0
47	34.81906388889863	37.0	33.0	40.0	27.0	41.0
48	34.84687430938742	36.0	33.0	40.0	27.0	41.0
49	34.85855202000891	36.0	33.0	39.0	27.0	41.0
50	34.615849273694806	36.0	33.0	39.0	26.0	41.0
51	33.603070090818846	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	3.0
12	5.0
13	5.0
14	7.0
15	11.0
16	22.0
17	39.0
18	105.0
19	263.0
20	517.0
21	869.0
22	1293.0
23	1657.0
24	2209.0
25	2782.0
26	3529.0
27	4473.0
28	5775.0
29	7613.0
30	10077.0
31	12363.0
32	15791.0
33	20048.0
34	26967.0
35	28220.0
36	32850.0
37	41015.0
38	46200.0
39	20362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.63582310495908	4.359234301389469	50.75752526545833	16.24741732819313
2	18.4023039712635	11.532133874481273	50.0941864013779	19.971375752877332
3	19.94611906423969	11.921507824311668	49.57361798556861	18.55875512588004
4	19.04143850873285	4.074394979531559	52.431482462386825	24.452684049348765
5	23.572207820453006	5.299695165799637	47.126174699112156	24.0019223146352
6	19.81106593749671	12.309127837431113	49.41436053221456	18.465445692857617
7	76.86838108133706	2.103671691110698	16.243207880086853	4.784739347465386
8	76.8434751800416	8.537813121551322	7.975501012021483	6.643210686385592
9	70.98041554268556	5.365993973473461	10.454164371932803	13.199426111908178
10	40.12516092369323	28.178396410743918	16.81814833393552	14.878294331627337
11	30.197528352386932	23.538532235602812	27.934599207922183	18.329340204088076
12	26.210128633718384	20.461776457258317	31.086423477495238	22.241671431528065
13	23.787942035899576	24.281149039018075	34.317174899060944	17.613734026021405
14	18.920767663019646	29.131134832130716	29.578388693422387	22.369708811427248
15	15.084908076176978	25.24897131611903	38.871797750049986	20.794322857654006
16	17.69371354004062	23.837753838490492	32.10300519516054	26.36552742630835
17	17.622503709576144	26.175049899499424	31.736783209914655	24.465663181009774
18	18.72502832607788	23.86932469928755	32.44186576771564	24.96378120691893
19	20.294100107691715	26.38096206936469	29.77518039239072	23.549757430552877
20	23.855994780284348	25.500135053126744	32.81650664917407	17.827363517414838
21	23.240713782083887	29.062731300403755	28.653362472068554	19.0431924454438
22	19.96471079337573	24.455841135428468	31.318293910682527	24.26115416051327
23	19.92787812244583	27.839185050846627	28.439382193332936	23.793554633374608
24	21.132832642866912	24.113823476793662	29.981443349598173	24.77190053074125
25	18.506838599235987	29.741504807540526	26.986771809326033	24.764884783897458
26	19.928579697130207	26.476025439098056	30.071946483883078	23.52344837988866
27	23.09899569583931	27.60941934171247	27.629765007559463	21.66181995488875
28	18.913751916175855	27.89145236483287	32.08336110399792	21.111434614993353
29	22.482311548270093	24.91642491572334	29.799384719001797	22.801878817004766
30	22.436007619101073	28.9841549357533	28.567770360574308	20.01206708457132
31	23.98648767157886	26.75840924956064	26.06490267405191	23.190200404808593
32	23.858099504337485	32.5102692994426	24.88695877897942	18.744672417240498
33	22.37567219624447	27.470156766863223	26.789629323015507	23.364541713876797
34	23.377871632879998	25.967734580265407	28.74561954306441	21.908774243790187
35	22.454950135579306	26.870310411719107	26.94993913839613	23.724800314305458
36	25.850220820631908	26.416742378268022	28.127532246126428	19.60550455497364
37	22.987796108365227	27.607314617659334	29.795526058237716	19.609363215737723
38	22.284116699932998	29.70818001003252	25.077436305788343	22.93026698424614
39	20.371974897657793	29.104474994124313	30.431854296969547	20.091695811248346
40	20.718903579083253	28.616880588480846	28.794378983628754	21.869836848807147
41	18.930238921258766	25.546088194953576	30.576729469293827	24.94694341449383
42	21.909475818474565	25.962823557474753	29.434916670466865	22.69278395358382
43	24.4112911429704	23.96088019559902	28.249957028550583	23.377871632879998
44	22.21501159352166	24.404275396126607	29.25355961455487	24.127153395796867
45	21.95262266156388	24.550553717819646	27.160411543709856	26.33641207690662
46	24.36358406443262	27.48874849599927	27.67396421267535	20.473703226892763
47	18.02590915309412	26.71035138368067	34.06320486331571	21.200534599909496
48	20.66663626509701	25.087258351369652	30.477105864111998	23.768999519421342
49	20.94375826542675	22.777674490393686	34.52378864361058	21.754778600568976
50	21.32331016967584	23.595359785037516	30.431503509627362	24.649826535659287
51	19.486938433313572	23.718135354803856	27.73780750895385	29.057118702928726
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44.0
1	144.5
2	245.0
3	405.5
4	566.0
5	438.5
6	311.0
7	331.5
8	352.0
9	420.5
10	489.0
11	515.0
12	541.0
13	543.0
14	545.0
15	592.0
16	639.0
17	673.0
18	707.0
19	684.0
20	661.0
21	959.0
22	1257.0
23	1327.5
24	1398.0
25	1764.0
26	2644.5
27	3159.0
28	3746.5
29	4334.0
30	4692.5
31	5051.0
32	5752.5
33	6454.0
34	7295.0
35	8136.0
36	8517.5
37	8899.0
38	9899.0
39	10899.0
40	12948.0
41	14997.0
42	17537.5
43	20078.0
44	30124.5
45	40171.0
46	37357.5
47	34544.0
48	32322.5
49	30101.0
50	25199.5
51	20298.0
52	17983.0
53	15668.0
54	13728.0
55	11788.0
56	10778.5
57	9769.0
58	9485.0
59	9201.0
60	8383.5
61	7566.0
62	6452.5
63	5339.0
64	4305.5
65	3272.0
66	2698.0
67	2124.0
68	1682.0
69	1240.0
70	1027.0
71	814.0
72	686.0
73	558.0
74	460.5
75	300.5
76	238.0
77	152.5
78	67.0
79	54.5
80	42.0
81	27.0
82	12.0
83	9.0
84	6.0
85	3.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	285073.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.96592024300126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.91188190443202	25.86377009098927
2	11.360676707189073	8.171943847145686
3	4.1160813363229	4.441159591676863
4	2.078941043219695	2.9908411100136543
5	1.2823220730317078	2.3059946702249214
6	1.0464454125623674	2.258182334812359
7	0.8765353833221446	2.206778118071295
8	0.7915804099048582	2.277593431084829
9	0.6846370966776291	2.2161242893056787
>10	5.700978101222738	34.41804579433351
>50	0.09395020012531773	2.293769519813795
>100	0.04397668942036149	2.649643493268685
>500	0.003997880856396499	1.1269342492738204
>1k	0.007995761712792999	6.779219459985638
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGC	4150	1.4557674700866094	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCG	4109	1.441385189056838	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	2749	0.9643144036790576	No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1850	0.6489565830506572	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGGAATATT	1717	0.6023018665394478	No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1709	0.5994955678019314	No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	1393	0.4886467676700354	No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1182	0.4146306384680415	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGGAATATTCGTA	873	0.3062373497314723	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTC	772	0.27080782817032834	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCT	769	0.26975546614375967	No Hit
GAACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT	721	0.25291767371866153	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGC	326	0.11435667355379148	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	290	0.10172832923496788	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC	286	0.10032517986620972	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5335475474702971	0.0
2	0.0	0.0	0.0	2.4274484079516476	0.0
3	0.0	0.0	0.0	3.028347125122337	0.0
4	0.0	0.0	0.0	4.158583941657049	0.0
5	0.0	0.0	0.0	6.492372129244089	0.0
6	0.0	0.0	0.0	7.2114861807326545	0.0
7	0.0	0.0	0.0	8.167381688199162	0.0
8	0.0	0.0	0.0	9.47827398596149	0.0
9	0.0	0.0	0.0	9.896763285193616	0.0
10	0.0	0.0	0.0	12.438217579356866	0.0
11	0.0	0.0	0.0	14.15356768266374	0.0
12	0.0	0.0	0.0	17.453424210640783	0.0
13	0.0	0.0	0.0	18.08834930000386	0.0
14	0.0	0.0	0.0	18.38792169023373	0.0
15	0.0	0.0	0.0	19.060030237868897	0.0
16	0.0	0.0	0.0	19.87982025656586	0.0
17	0.0	0.0	0.0	21.050046830110183	0.0
18	0.0	0.0	0.0	22.285870636643946	0.0
19	0.0	0.0	0.0	23.83354439038422	0.0
20	0.0	0.0	0.0	24.735418647153537	0.0
21	0.0	0.0	0.0	25.79584878259253	0.0
22	0.0	0.0	0.0	26.88188639401136	0.0
23	0.0	0.0	0.0	27.90618543320483	0.0
24	0.0	0.0	0.0	28.691949079709406	0.0
25	0.0	0.0	0.0	29.388261953955652	0.0
26	0.0	0.0	0.0	30.024940980029676	0.0
27	0.0	0.0	0.0	30.651447173180202	0.0
28	0.0	0.0	0.0	31.31794312334034	0.0
29	0.0	0.0	0.0	31.996716630477106	0.0
30	0.0	0.0	0.0	32.76774720860973	0.0
31	0.0	0.0	0.0	33.53421755129388	0.0
32	0.0	0.0	0.0	34.230530425540124	0.0
33	0.0	0.0	0.0	34.868261813640714	0.0
34	0.0	0.0	0.0	35.54773689546187	0.0
35	0.0	0.0	0.0	36.26334307352853	0.0
36	0.0	0.0	0.0	36.97473980348893	0.0
37	0.0	0.0	0.0	37.688592044844654	0.0
38	0.0	0.0	0.0	38.377538384904916	0.0
39	0.0	0.0	0.0	39.080165431310576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCC	20	7.024351E-4	45.000004	30
GAGCTAG	20	7.024351E-4	45.000004	45
CCGATCG	20	7.024351E-4	45.000004	40
TTAGCGT	20	7.024351E-4	45.000004	28
CGGCATG	20	7.024351E-4	45.000004	5
TAGCGTC	20	7.024351E-4	45.000004	29
CGATTCG	20	7.024351E-4	45.000004	10
CCTCCGC	20	7.024351E-4	45.000004	45
TACGAGA	20	7.024351E-4	45.000004	35
ACGGATG	20	7.024351E-4	45.000004	20
CTGCCCG	20	7.024351E-4	45.000004	1
GACGGAT	20	7.024351E-4	45.000004	19
TCATCGA	30	2.1597389E-6	45.000004	16
CATCGAC	30	2.1597389E-6	45.000004	17
ACCAACG	30	2.1597389E-6	45.000004	36
TTCATCG	30	2.1597389E-6	45.000004	15
GTCCAAC	20	7.024351E-4	45.000004	8
CGATCGA	20	7.024351E-4	45.000004	41
CTTAGTT	20	7.024351E-4	45.000004	26
TCGACAA	30	2.1597389E-6	45.000004	19
>>END_MODULE