FastQCFastQC Report
Sat 18 Jun 2016
SRR3549635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234337
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC15020.6409572538694274No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC11010.46983617610535255No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG10830.4621549307194339No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT8660.3695532502336379Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC7190.3068230795819696No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC6710.2863397585528534No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC5430.23171756914187688No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTGTGGT5370.22915715401323733No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG2590.11052458638627276No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2530.10796417125763325No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.10711069954808673No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTA2460.10497702027422046No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC2430.1036968127099007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTCG406.7757355E-945.0000041
ACAGGCC207.0217793E-445.00000413
TGATTGA207.0217793E-445.0000046
AGGCACG207.0217793E-445.00000410
CGAGGGC207.0217793E-445.0000044
ATCCAGT351.2063902E-745.00000411
GATTGAA207.0217793E-445.0000047
GAACCTA207.0217793E-445.00000423
TTGTTCG406.7757355E-945.0000041
GGTCTAT207.0217793E-445.0000048
CACCGGT207.0217793E-445.00000438
AAGGCGT207.0217793E-445.00000414
TGACTAG207.0217793E-445.00000428
TGCAAGC207.0217793E-445.00000413
TTCCGCG253.881271E-545.01
GGATAAG253.881271E-545.08
TACTGCG253.881271E-545.01
CGGGATC502.1827873E-1145.06
GTACGGG253.881271E-545.03
TTTGGGC4450.043.4831474