##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549635_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	234337
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.068269202046626	31.0	30.0	31.0	26.0	34.0
2	30.200984052881108	31.0	30.0	33.0	26.0	34.0
3	30.328868253839556	31.0	30.0	34.0	26.0	34.0
4	34.06226076121142	35.0	33.0	37.0	30.0	37.0
5	34.49033656656866	35.0	35.0	37.0	32.0	37.0
6	33.96699198163329	35.0	35.0	37.0	30.0	37.0
7	34.499507120087735	35.0	35.0	37.0	32.0	37.0
8	34.452502165684464	35.0	35.0	37.0	31.0	37.0
9	35.88831895944729	37.0	35.0	39.0	31.0	39.0
10	35.39054438692993	37.0	34.0	39.0	30.0	39.0
11	35.51947835809113	37.0	35.0	39.0	30.0	39.0
12	35.42369749548727	37.0	35.0	39.0	30.0	39.0
13	35.26663309677942	37.0	34.0	39.0	30.0	39.0
14	35.96770463051076	38.0	34.0	40.0	29.0	41.0
15	36.30439495256831	38.0	35.0	40.0	30.0	41.0
16	36.51042729061138	38.0	35.0	40.0	31.0	41.0
17	36.30765948185733	38.0	35.0	40.0	30.0	41.0
18	36.41411300818906	38.0	35.0	40.0	30.0	41.0
19	36.38461702590713	38.0	35.0	40.0	30.0	41.0
20	36.37042379137738	38.0	35.0	40.0	30.0	41.0
21	36.10293295552986	38.0	34.0	40.0	30.0	41.0
22	36.28860145858315	38.0	34.0	40.0	30.0	41.0
23	36.34486658103501	38.0	35.0	40.0	30.0	41.0
24	36.259651698195334	38.0	35.0	40.0	30.0	41.0
25	35.808067014598635	38.0	34.0	40.0	29.0	41.0
26	35.84186876165522	38.0	34.0	40.0	30.0	41.0
27	35.83096139320722	38.0	34.0	40.0	29.0	41.0
28	35.710822448012905	38.0	34.0	40.0	29.0	41.0
29	35.864639386865925	38.0	34.0	40.0	30.0	41.0
30	35.491160166768374	38.0	34.0	40.0	28.0	41.0
31	35.634355650196085	38.0	34.0	40.0	29.0	41.0
32	35.64639386865924	38.0	34.0	40.0	29.0	41.0
33	35.62093054020492	38.0	34.0	40.0	29.0	41.0
34	35.5210316766025	38.0	34.0	40.0	29.0	41.0
35	35.58150014722387	38.0	34.0	40.0	29.0	41.0
36	35.32994789555213	38.0	34.0	40.0	27.0	41.0
37	35.351638879050256	38.0	34.0	40.0	28.0	41.0
38	35.291865987872164	37.0	34.0	40.0	28.0	41.0
39	35.240158404349295	37.0	34.0	40.0	28.0	41.0
40	35.14501337816905	37.0	34.0	40.0	27.0	41.0
41	35.001297276998514	37.0	33.0	40.0	27.0	41.0
42	35.24206591362013	37.0	34.0	40.0	28.0	41.0
43	35.1876357553438	37.0	34.0	40.0	28.0	41.0
44	35.11700243666173	37.0	34.0	40.0	27.0	41.0
45	35.205639741056686	37.0	34.0	40.0	28.0	41.0
46	35.133197062350376	37.0	34.0	40.0	28.0	41.0
47	35.020030981023055	37.0	34.0	40.0	27.0	41.0
48	35.054891032999485	37.0	34.0	40.0	28.0	41.0
49	35.04988542142299	37.0	34.0	40.0	28.0	41.0
50	34.83377784984872	37.0	33.0	40.0	27.0	41.0
51	33.78570178844997	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	3.0
12	1.0
13	1.0
14	7.0
15	17.0
16	29.0
17	48.0
18	88.0
19	182.0
20	382.0
21	611.0
22	943.0
23	1250.0
24	1702.0
25	2198.0
26	2790.0
27	3512.0
28	4415.0
29	5750.0
30	7395.0
31	9476.0
32	12093.0
33	15996.0
34	22440.0
35	23089.0
36	26486.0
37	33858.0
38	39969.0
39	19602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.35994742614269	4.44061330477048	52.09377947144497	20.10565979764186
2	18.461019813345736	6.900745507538289	54.28293440643176	20.35530027268421
3	21.297106304168782	6.252107008282944	51.384117744957045	21.066668942591228
4	19.42800326026193	4.628803816725485	48.757558558827675	27.185634364184914
5	20.019885890832434	5.539884866666382	48.182745362448095	26.257483880053083
6	21.28259728510649	8.244109978364492	51.761352240576606	18.711940495952412
7	82.06301181631582	2.490857184311483	10.944921203224416	4.501209796148283
8	82.68391248501091	5.001344217942536	6.76589697742994	5.5488463196166204
9	76.96437182348498	5.539458130811608	9.71122784707494	7.784942198628471
10	41.05796353115385	27.656750747854588	16.53772131588268	14.747564405108882
11	33.68823531921976	25.372433717253358	23.398780388927058	17.540550574599827
12	30.7326627890602	20.816174995839326	27.883774222593953	20.567387992506518
13	25.57214609728724	25.398037868539753	29.303097675569802	19.726718358603208
14	18.86641887538033	29.4003934504581	28.728711215045	23.00447645911657
15	16.86033362209126	25.760763345097022	37.044939552866175	20.33396347994555
16	18.743945685060403	22.686558247310497	34.743553088074016	23.825942979555084
17	18.76826962878248	24.203604211029415	30.7262617512386	26.301864408949506
18	20.418457179190654	23.854534281824893	31.06978411433107	24.657224424653386
19	23.847706508148523	26.12306208579951	27.509526877957814	22.519704528094156
20	28.539240495525675	24.658077896362933	28.41634056935098	18.386341038760417
21	25.246119904240473	26.94922270064053	27.206971156923576	20.59768623819542
22	22.535493754720765	24.230915305734904	27.536837972663303	25.696752966881032
23	22.844023777721826	27.03841049428814	26.5045639399668	23.61300178802323
24	23.54984488151679	23.386405049138634	29.165261994478037	23.89848807486654
25	20.81446805242023	27.520622010181917	26.797731472196023	24.867178465201825
26	21.106355377085137	27.99429880898023	28.094581734851943	22.80476407908269
27	22.725817945949636	28.14749698084383	27.7873319194152	21.339353153791336
28	18.30398101878918	28.097568885835354	32.351698622069925	21.24675147330554
29	20.97662767723407	25.903719856446056	30.866657847459	22.25299461886087
30	23.149566649739477	25.967303498807276	29.272372694026128	21.610757157427123
31	22.93747892991717	26.7247596410298	27.52104874603669	22.81671268301634
32	26.129889859475885	27.668272615933464	26.172563444953205	20.029274079637446
33	27.214225666454723	25.865313629516468	25.602444342976145	21.31801636105267
34	22.171488070599178	27.05206604164088	28.560577288264337	22.215868599495597
35	23.670611128417622	24.795060105745144	27.576097671302442	23.958231094534792
36	24.737450765350754	27.73441667342332	26.85064671818791	20.677485843038017
37	22.546162151090098	26.863448793831108	30.511186880432884	20.079202174645914
38	22.381015375292847	26.296316842837452	27.92644780807128	23.39621997379842
39	22.941746288464905	24.801887879421518	30.734369732479294	21.52199609963429
40	25.2153949226968	23.7794287713848	29.353452506433044	21.651723799485357
41	20.511485595531223	25.40699932148999	29.58132945288196	24.500185630096826
42	24.60558938622582	24.897049975035955	27.215932609873818	23.28142802886441
43	25.16290641255969	24.845841672463163	27.229161421371785	22.762090493605363
44	21.789559480577118	25.753508835565874	29.071806842282694	23.385124841574314
45	21.815590367718286	25.40315869879703	27.28762423347572	25.49362670000896
46	23.34245125609699	26.720065546627293	27.90255060020398	22.034932597071737
47	20.7368021268515	26.458476467651288	31.453846383627	21.350875021870213
48	23.727366997102465	23.51314559800629	29.99825038299543	22.761237021895816
49	23.177731216154513	22.33151401613915	31.777312161545122	22.713442606161212
50	22.034505861216967	23.77516141283707	29.546763848645323	24.64356887730064
51	20.998391205827506	24.15580979529481	27.281649931508895	27.56414906736879
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	53.0
1	153.0
2	253.0
3	418.0
4	583.0
5	466.5
6	350.0
7	323.0
8	296.0
9	379.0
10	462.0
11	474.0
12	486.0
13	498.5
14	511.0
15	492.5
16	474.0
17	447.5
18	421.0
19	550.0
20	679.0
21	722.0
22	765.0
23	900.5
24	1036.0
25	1107.0
26	1758.0
27	2338.0
28	2744.5
29	3151.0
30	3563.5
31	3976.0
32	4562.5
33	5149.0
34	5853.5
35	6558.0
36	7097.5
37	7637.0
38	8143.5
39	8650.0
40	10607.5
41	12565.0
42	13428.0
43	14291.0
44	15441.5
45	16592.0
46	19984.0
47	23376.0
48	23100.0
49	22824.0
50	22223.5
51	21623.0
52	18686.5
53	15750.0
54	13926.5
55	12103.0
56	11737.5
57	11372.0
58	10664.0
59	9956.0
60	9775.5
61	9595.0
62	8409.5
63	7224.0
64	6100.5
65	4977.0
66	3893.0
67	2809.0
68	2280.5
69	1752.0
70	1399.0
71	1046.0
72	829.5
73	613.0
74	511.5
75	348.5
76	287.0
77	198.5
78	110.0
79	76.0
80	42.0
81	27.5
82	13.0
83	6.5
84	0.0
85	0.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	234337.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.268549140767355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.36393615315914	27.714360088248974
2	9.297638949320998	6.9301902815176435
3	3.247303455698811	3.630668652410844
4	1.7702154945382096	2.638934525917802
5	1.159914809810612	2.161417104426531
6	1.0190761902580896	2.278769464489176
7	0.8851077472691048	2.309067710178077
8	0.8644972175784917	2.5774845628304535
9	0.7854901870978084	2.6346671673700692
>10	6.4923168525431105	40.52326350512296
>50	0.07900703048068336	1.962984931956968
>100	0.02633567682689445	1.6416528333127078
>500	0.005725147136281403	1.423590811523575
>1k	0.003435088281768841	1.5729483606942138
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1502	0.6409572538694274	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	1101	0.46983617610535255	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	1083	0.4621549307194339	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	866	0.3695532502336379	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	719	0.3068230795819696	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	671	0.2863397585528534	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	543	0.23171756914187688	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTGTGGT	537	0.22915715401323733	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	259	0.11052458638627276	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	253	0.10796417125763325	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	251	0.10711069954808673	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTA	246	0.10497702027422046	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	243	0.1036968127099007	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4053990620345912	0.0
2	0.0	0.0	0.0	1.744496174313062	0.0
3	0.0	0.0	0.0	2.157576481733572	0.0
4	0.0	0.0	0.0	2.786585131669348	0.0
5	0.0	0.0	0.0	3.9878465628560575	0.0
6	0.0	0.0	0.0	4.499929588583963	0.0
7	0.0	0.0	0.0	5.037190029743489	0.0
8	0.0	0.0	0.0	5.9410165701532405	0.0
9	0.0	0.0	0.0	6.281125046407524	0.0
10	0.0	0.0	0.0	7.43587226942395	0.0
11	0.0	0.0	0.0	8.691329154166862	0.0
12	0.0	0.0	0.0	10.400832988388517	0.0
13	0.0	0.0	0.0	10.848052164190888	0.0
14	0.0	0.0	0.0	11.022587128793148	0.0
15	0.0	0.0	0.0	11.371230322142896	0.0
16	0.0	0.0	0.0	11.948603933651109	0.0
17	0.0	0.0	0.0	12.766656567251436	0.0
18	0.0	0.0	0.0	13.75455007105152	0.0
19	0.0	0.0	0.0	14.56108083657297	0.0
20	0.0	0.0	0.0	15.228922449293112	0.0
21	0.0	0.0	0.0	16.045268139474345	0.0
22	0.0	0.0	0.0	16.844544395464652	0.0
23	0.0	0.0	0.0	17.693748746463427	0.0
24	0.0	0.0	0.0	18.337266415461492	0.0
25	0.0	0.0	0.0	18.89287649837627	0.0
26	0.0	0.0	0.0	19.407946675087587	0.0
27	0.0	0.0	0.0	19.90509394589843	0.0
28	0.0	0.0	0.0	20.444914802186595	0.0
29	0.0	0.0	0.0	21.045758885707336	0.0
30	0.0	0.0	0.0	21.707199460605878	0.0
31	0.0	0.0	0.0	22.31871194049595	0.0
32	0.0	0.0	0.0	22.92937094867648	0.0
33	0.0	0.0	0.0	23.46065708786918	0.0
34	0.0	0.0	0.0	24.050406039165818	0.0
35	0.0	0.0	0.0	24.685388991068418	0.0
36	0.0	0.0	0.0	25.30756986732782	0.0
37	0.0	0.0	0.0	25.969010442226367	0.0
38	0.0	0.0	0.0	26.569854525747107	0.0
39	0.0	0.0	0.0	27.167284722429663	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTCG	40	6.7757355E-9	45.000004	1
ACAGGCC	20	7.0217793E-4	45.000004	13
TGATTGA	20	7.0217793E-4	45.000004	6
AGGCACG	20	7.0217793E-4	45.000004	10
CGAGGGC	20	7.0217793E-4	45.000004	4
ATCCAGT	35	1.2063902E-7	45.000004	11
GATTGAA	20	7.0217793E-4	45.000004	7
GAACCTA	20	7.0217793E-4	45.000004	23
TTGTTCG	40	6.7757355E-9	45.000004	1
GGTCTAT	20	7.0217793E-4	45.000004	8
CACCGGT	20	7.0217793E-4	45.000004	38
AAGGCGT	20	7.0217793E-4	45.000004	14
TGACTAG	20	7.0217793E-4	45.000004	28
TGCAAGC	20	7.0217793E-4	45.000004	13
TTCCGCG	25	3.881271E-5	45.0	1
GGATAAG	25	3.881271E-5	45.0	8
TACTGCG	25	3.881271E-5	45.0	1
CGGGATC	50	2.1827873E-11	45.0	6
GTACGGG	25	3.881271E-5	45.0	3
TTTGGGC	445	0.0	43.483147	4
>>END_MODULE