##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549633_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	264464
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.00451857341642	31.0	30.0	31.0	26.0	34.0
2	30.381095347570938	31.0	30.0	33.0	27.0	34.0
3	30.383761116824974	31.0	30.0	34.0	26.0	34.0
4	34.01842594833323	35.0	33.0	37.0	30.0	37.0
5	34.38057353741908	35.0	35.0	37.0	32.0	37.0
6	33.91036965333656	35.0	35.0	37.0	30.0	37.0
7	34.41090658842035	35.0	35.0	37.0	32.0	37.0
8	34.58332703127836	35.0	35.0	37.0	32.0	37.0
9	35.82795011797447	37.0	35.0	39.0	30.0	39.0
10	35.20632675902959	37.0	34.0	39.0	30.0	39.0
11	35.38224484239821	37.0	35.0	39.0	30.0	39.0
12	35.393546947788735	37.0	35.0	39.0	30.0	39.0
13	35.38037313207091	37.0	35.0	39.0	30.0	39.0
14	35.988028616371224	38.0	34.0	40.0	29.0	41.0
15	36.30059667856494	38.0	35.0	40.0	30.0	41.0
16	36.439458678685945	38.0	35.0	40.0	31.0	41.0
17	36.26761676447456	38.0	35.0	40.0	30.0	41.0
18	36.36051409643657	38.0	35.0	40.0	30.0	41.0
19	36.38490683041926	38.0	35.0	40.0	30.0	41.0
20	36.44312647468087	38.0	35.0	40.0	30.0	41.0
21	36.08993662653518	38.0	34.0	40.0	30.0	41.0
22	36.26145713594289	38.0	34.0	40.0	30.0	41.0
23	36.34079874765563	38.0	35.0	40.0	30.0	41.0
24	36.23460281928731	38.0	34.0	40.0	30.0	41.0
25	35.73667115372981	38.0	34.0	40.0	29.0	41.0
26	35.86887440256519	38.0	34.0	40.0	29.0	41.0
27	35.9288182890677	38.0	34.0	40.0	30.0	41.0
28	35.80606056022748	38.0	34.0	40.0	29.0	41.0
29	35.99345090447093	38.0	34.0	40.0	30.0	41.0
30	35.467004960977675	38.0	34.0	40.0	28.0	41.0
31	35.652644594349326	38.0	34.0	40.0	29.0	41.0
32	35.74497096013068	38.0	34.0	40.0	29.0	41.0
33	35.74172288099704	38.0	34.0	40.0	29.0	41.0
34	35.753853076411154	38.0	34.0	40.0	29.0	41.0
35	35.82068258817835	38.0	34.0	40.0	29.0	41.0
36	35.57056915118882	38.0	34.0	40.0	29.0	41.0
37	35.654331024260394	38.0	34.0	40.0	29.0	41.0
38	35.523284832718254	38.0	34.0	40.0	29.0	41.0
39	35.48656149797326	38.0	34.0	40.0	29.0	41.0
40	35.42739654546555	38.0	34.0	40.0	28.0	41.0
41	35.36612544618549	38.0	34.0	40.0	28.0	41.0
42	35.581451539718074	38.0	34.0	40.0	29.0	41.0
43	35.556669338737976	38.0	34.0	40.0	29.0	41.0
44	35.456742694657876	38.0	34.0	40.0	28.0	41.0
45	35.58977781474984	38.0	34.0	40.0	29.0	41.0
46	35.48159673906468	38.0	34.0	40.0	29.0	41.0
47	35.44274835138242	37.0	34.0	40.0	28.0	41.0
48	35.43107568515941	37.0	34.0	40.0	28.0	41.0
49	35.45205396575715	37.0	34.0	40.0	28.0	41.0
50	35.30100883296025	37.0	34.0	40.0	28.0	41.0
51	34.19981169459737	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	7.0
12	7.0
13	7.0
14	5.0
15	14.0
16	38.0
17	48.0
18	101.0
19	170.0
20	284.0
21	493.0
22	753.0
23	1120.0
24	1604.0
25	2175.0
26	3027.0
27	3839.0
28	4991.0
29	6357.0
30	8367.0
31	10493.0
32	13419.0
33	17658.0
34	24352.0
35	26199.0
36	31387.0
37	39381.0
38	46918.0
39	21244.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.494040776816504	4.9696745114646985	57.01191844636699	16.524366265351805
2	20.802831387258756	3.7025833383749776	53.42315022082401	22.07143505354226
3	20.941980761086576	3.6617460221429003	53.4700375098312	21.926235706939316
4	20.091203339584972	4.9696745114646985	46.916026377881295	28.02309577106903
5	17.97635973138121	6.289324823038296	50.30930485812814	25.425010587452356
6	22.032110230503964	6.556279871740577	52.920246233891945	18.491363663863513
7	81.63114828483272	2.971671002480489	10.343562828967269	5.053617883719523
8	82.49780688486902	2.9637304132131406	8.054782503478734	6.483680198439107
9	76.31511283199227	6.148284832718253	11.169005989473048	6.367596345816444
10	36.880634037146834	30.029417992619035	18.333686248411883	14.756261721822254
11	28.794467299897153	26.324565914453384	26.358218888015006	18.52274789763446
12	26.00845483695323	22.768694415875128	31.600898420957108	19.621952326214533
13	23.133583398874705	26.32418779115494	31.5974953112711	18.944733498699257
14	19.068001693992375	28.93626353681408	31.082113255490352	20.913621513703188
15	16.848417932119304	27.798112408494163	35.885791638937626	19.467678020448908
16	19.008636336136487	26.390359368382843	32.940211144049854	21.66079315143082
17	19.397725210236555	25.68515941678263	28.58120575957408	26.335909613406738
18	19.982681952931213	25.59025046887289	31.949906225421987	22.477161352773912
19	22.259740456167947	26.78474196866114	29.537479581341884	21.418037993829028
20	24.14733196200617	27.66387863754613	29.036088087603606	19.15270131284409
21	23.562375219311512	26.751845241696415	29.815022082400628	19.870757456591445
22	21.551893641478614	26.59908342912457	28.558518361667375	23.290504567729446
23	20.194052876762054	27.87562768467542	28.083217375521812	23.84710206304072
24	21.61995583519874	25.307036118337468	30.414725633734648	22.658282412729143
25	19.584139996370016	27.456288946699743	27.713790912940894	25.245780143989354
26	18.203233710448302	29.410052029765865	28.553224635489137	23.833489624296693
27	19.015064432210053	29.646000967995644	29.77531913606389	21.56361546373041
28	16.573522294149676	28.96878214048037	31.486327061528225	22.97136850384173
29	17.749863875612558	25.875355435900538	30.85259241333414	25.522188275152764
30	18.78365297356162	28.397059713231293	30.175373585818864	22.643913727388227
31	20.13355314901083	29.8014096436566	27.247564885958013	22.817472321374556
32	19.430243813902838	29.37791154939803	26.230791336438987	24.96105330026015
33	19.509649706576322	30.4653941557263	27.24227115977978	22.7826849779176
34	17.609958255187852	27.861258999334503	29.6221792001936	24.906603545284046
35	16.952023715893276	27.78109686006413	27.532669852985663	27.73420957105693
36	18.86494948272733	29.185068667191	28.6734678443947	23.276514005686973
37	17.622436324036542	29.63919474862363	30.492619033214353	22.245749894125478
38	17.457574565914452	30.9259483332325	26.47165587754855	25.144821223304497
39	18.867218222517998	29.10604089781596	28.47344061951721	23.55330026014883
40	19.554646379091295	27.28121785951963	29.955305826123784	23.20882993526529
41	16.803799382902778	26.872466573900418	28.92567608445762	27.398057958739187
42	18.40401718192268	27.37612680742937	27.73345332446004	26.48640268618791
43	19.750514247685885	27.989820920805858	27.57199467602396	24.6876701554843
44	17.57970839131224	28.28059773731018	27.995870893580978	26.1438229777966
45	18.839237098433056	27.24000241998911	26.060635852138663	27.86012462943917
46	19.37390344243451	27.707362816867327	28.906769919535364	24.011963821162805
47	16.332279629741667	27.782987476556354	30.865826728779723	25.018906164922257
48	16.421516728174723	26.74655151551818	30.626474680863936	26.20545707544316
49	18.219114888982997	25.86060862726118	31.49993950027225	24.420336983483573
50	18.570769556536995	25.390601367293847	29.400598947304736	26.638030128864422
51	16.50999758001089	25.871952326214533	27.147362211870046	30.470687881904528
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63.0
1	136.0
2	209.0
3	416.5
4	624.0
5	490.0
6	356.0
7	416.5
8	477.0
9	652.0
10	827.0
11	951.5
12	1076.0
13	1116.5
14	1157.0
15	1148.0
16	1139.0
17	997.5
18	856.0
19	911.5
20	967.0
21	1028.5
22	1090.0
23	1378.0
24	1666.0
25	1664.5
26	2213.5
27	2764.0
28	3166.5
29	3569.0
30	4294.0
31	5019.0
32	5828.5
33	6638.0
34	6889.0
35	7140.0
36	8393.0
37	9646.0
38	10785.5
39	11925.0
40	13567.5
41	15210.0
42	18023.5
43	20837.0
44	24060.0
45	27283.0
46	29285.0
47	31287.0
48	30105.5
49	28924.0
50	24766.0
51	20608.0
52	17685.5
53	14763.0
54	12876.5
55	10990.0
56	10079.5
57	9169.0
58	8484.0
59	7799.0
60	7096.5
61	6394.0
62	5403.5
63	4413.0
64	3785.0
65	3157.0
66	2489.5
67	1822.0
68	1466.0
69	1110.0
70	947.5
71	785.0
72	623.0
73	461.0
74	351.5
75	192.0
76	142.0
77	150.0
78	158.0
79	89.5
80	21.0
81	15.5
82	10.0
83	5.5
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	3.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	264464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.65968112471965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.5676882222077	28.383516937261117
2	11.51149479546126	9.13084425713727
3	4.209075939344027	5.007918287523418
4	2.2095259944226378	3.5051638550232362
5	1.482382107295613	2.9395400840166985
6	1.2018491703807246	2.8598972914385037
7	0.9532912498325848	2.646505889114204
8	0.784593361817209	2.489337803379384
9	0.7546470181788132	2.693615409242302
>10	5.196683007765183	33.61437234693888
>50	0.08384966819160689	2.2058825649188094
>100	0.040926623760189074	2.701137247028183
>500	9.982103356143677E-4	0.3246273695680946
>1k	0.0029946310068431027	1.4976406574098806
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	1501	0.5675630709661806	No Hit
TCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	1278	0.4832415754129106	No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT	1004	0.3796357916389376	No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	820	0.31006110472502874	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCC	409	0.1546524290640692	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT	328	0.12402444189001151	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	315	0.11910883901022447	No Hit
TGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG	292	0.11041200314598584	No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG	278	0.10511827696775364	No Hit
TTCCTTGGGATTTTCTGTTTTAATCTTTTCTTTTTAAACGAGGACTCATAC	272	0.1028495371770827	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.41064190211144047	0.0
2	0.0	0.0	0.0	1.95111621997701	0.0
3	0.0	0.0	0.0	2.58901022445399	0.0
4	0.0	0.0	0.0	3.5880119789460947	0.0
5	0.0	0.0	0.0	5.0562647468086395	0.0
6	0.0	0.0	0.0	5.826880029039869	0.0
7	0.0	0.0	0.0	6.625476435356041	0.0
8	0.0	0.0	0.0	7.836227236977433	0.0
9	0.0	0.0	0.0	8.37127170427733	0.0
10	0.0	0.0	0.0	9.396363966362152	0.0
11	0.0	0.0	0.0	11.40003932482304	0.0
12	0.0	0.0	0.0	13.277043378304798	0.0
13	0.0	0.0	0.0	13.874100066549701	0.0
14	0.0	0.0	0.0	14.075261661322523	0.0
15	0.0	0.0	0.0	14.411791396938714	0.0
16	0.0	0.0	0.0	15.2482001330994	0.0
17	0.0	0.0	0.0	16.511131949906225	0.0
18	0.0	0.0	0.0	17.97484723818743	0.0
19	0.0	0.0	0.0	18.88423377094803	0.0
20	0.0	0.0	0.0	19.841263839312724	0.0
21	0.0	0.0	0.0	21.05390525742634	0.0
22	0.0	0.0	0.0	22.247640510617703	0.0
23	0.0	0.0	0.0	23.382010405953174	0.0
24	0.0	0.0	0.0	24.32769677536451	0.0
25	0.0	0.0	0.0	25.144064976707604	0.0
26	0.0	0.0	0.0	25.917705245326395	0.0
27	0.0	0.0	0.0	26.618367717345272	0.0
28	0.0	0.0	0.0	27.383311150099825	0.0
29	0.0	0.0	0.0	28.162623268195293	0.0
30	0.0	0.0	0.0	29.01075382660778	0.0
31	0.0	0.0	0.0	29.875521810151856	0.0
32	0.0	0.0	0.0	30.635927763325064	0.0
33	0.0	0.0	0.0	31.37818379817291	0.0
34	0.0	0.0	0.0	32.13140540867566	0.0
35	0.0	0.0	0.0	32.9095831568758	0.0
36	0.0	0.0	0.0	33.66318289067699	0.0
37	0.0	0.0	0.0	34.418295117671974	0.0
38	0.0	0.0	0.0	35.173785467965395	0.0
39	0.0	0.0	0.0	35.95082884627019	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	20	7.0234254E-4	45.000004	15
GTACCTC	20	7.0234254E-4	45.000004	30
TCGTTCA	20	7.0234254E-4	45.000004	16
CCAATGA	20	7.0234254E-4	45.000004	35
TTCCGCG	30	2.1591422E-6	45.000004	1
CTGGTCG	20	7.0234254E-4	45.000004	1
TAGTTGG	20	7.0234254E-4	45.000004	2
GTATGAC	20	7.0234254E-4	45.000004	18
CGCTAGG	40	6.7811925E-9	45.000004	2
TACGAAA	30	2.1591422E-6	45.000004	20
GGTATGC	30	2.1591422E-6	45.000004	8
TTGCGCG	20	7.0234254E-4	45.000004	1
TTGCGAT	20	7.0234254E-4	45.000004	28
CATACGA	30	2.1591422E-6	45.000004	18
GCGATCT	60	0.0	45.000004	9
AGTTCCG	20	7.0234254E-4	45.000004	1
TCCGTAG	20	7.0234254E-4	45.000004	1
CGAGATG	20	7.0234254E-4	45.000004	18
ACACTTA	30	2.1591422E-6	45.000004	29
GATCTAC	20	7.0234254E-4	45.000004	9
>>END_MODULE