FastQCFastQC Report
Sat 18 Jun 2016
SRR3549630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167790
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC19551.165146909827761TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT12920.7700101317122594TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC11680.6961082305262531TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC9530.5679718695989034TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG3570.2127659574468085No Hit
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG3560.2121699743727278No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC3150.18773466833541927No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC3050.1817748375946123No Hit
TTCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT3040.18117885452053162No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.16389534537219141No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC2390.14243995470528636No Hit
TGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG2220.13230824244591452No Hit
CGTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC1870.11144883485309016No Hit
TCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG1720.10250908874187972No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAC207.0160505E-445.00000439
CTGTCGG207.0160505E-445.0000042
TCACCGA207.0160505E-445.00000413
CTACTGG351.2036435E-745.0000042
GGATGCA406.7557266E-945.0000048
TTTCGCG207.0160505E-445.0000041
ACCGAGT207.0160505E-445.00000415
GCCATTC207.0160505E-445.00000435
AGGCGCA207.0160505E-445.00000423
GCGCATA207.0160505E-445.00000425
ACAGGCG207.0160505E-445.00000433
ATCCCTA207.0160505E-445.00000421
CGGGCAC351.2036435E-745.0000046
TAGGGAT207.0160505E-445.0000045
AGTTCGT207.0160505E-445.00000419
TTCGGTG207.0160505E-445.0000041
CGATGTG207.0160505E-445.00000410
TAGGCGC207.0160505E-445.00000422
CACACCG207.0160505E-445.00000417
GGCGCAT207.0160505E-445.00000424