Basic Statistics
Measure | Value |
---|---|
Filename | SRR3549630_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 167790 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC | 1955 | 1.165146909827761 | TruSeq Adapter, Index 15 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT | 1292 | 0.7700101317122594 | TruSeq Adapter, Index 21 (95% over 22bp) |
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC | 1168 | 0.6961082305262531 | TruSeq Adapter, Index 15 (95% over 22bp) |
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC | 953 | 0.5679718695989034 | TruSeq Adapter, Index 15 (95% over 22bp) |
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG | 357 | 0.2127659574468085 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG | 356 | 0.2121699743727278 | No Hit |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC | 315 | 0.18773466833541927 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC | 305 | 0.1817748375946123 | No Hit |
TTCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT | 304 | 0.18117885452053162 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 275 | 0.16389534537219141 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC | 239 | 0.14243995470528636 | No Hit |
TGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG | 222 | 0.13230824244591452 | No Hit |
CGTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC | 187 | 0.11144883485309016 | No Hit |
TCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG | 172 | 0.10250908874187972 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCATAC | 20 | 7.0160505E-4 | 45.000004 | 39 |
CTGTCGG | 20 | 7.0160505E-4 | 45.000004 | 2 |
TCACCGA | 20 | 7.0160505E-4 | 45.000004 | 13 |
CTACTGG | 35 | 1.2036435E-7 | 45.000004 | 2 |
GGATGCA | 40 | 6.7557266E-9 | 45.000004 | 8 |
TTTCGCG | 20 | 7.0160505E-4 | 45.000004 | 1 |
ACCGAGT | 20 | 7.0160505E-4 | 45.000004 | 15 |
GCCATTC | 20 | 7.0160505E-4 | 45.000004 | 35 |
AGGCGCA | 20 | 7.0160505E-4 | 45.000004 | 23 |
GCGCATA | 20 | 7.0160505E-4 | 45.000004 | 25 |
ACAGGCG | 20 | 7.0160505E-4 | 45.000004 | 33 |
ATCCCTA | 20 | 7.0160505E-4 | 45.000004 | 21 |
CGGGCAC | 35 | 1.2036435E-7 | 45.000004 | 6 |
TAGGGAT | 20 | 7.0160505E-4 | 45.000004 | 5 |
AGTTCGT | 20 | 7.0160505E-4 | 45.000004 | 19 |
TTCGGTG | 20 | 7.0160505E-4 | 45.000004 | 1 |
CGATGTG | 20 | 7.0160505E-4 | 45.000004 | 10 |
TAGGCGC | 20 | 7.0160505E-4 | 45.000004 | 22 |
CACACCG | 20 | 7.0160505E-4 | 45.000004 | 17 |
GGCGCAT | 20 | 7.0160505E-4 | 45.000004 | 24 |