##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549630_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	167790
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.00746766791823	31.0	30.0	31.0	26.0	34.0
2	30.372477501638954	31.0	30.0	33.0	27.0	34.0
3	30.353870910066153	31.0	30.0	34.0	26.0	34.0
4	34.04725549794386	35.0	33.0	37.0	30.0	37.0
5	34.391686036116575	35.0	35.0	37.0	32.0	37.0
6	33.986089755050955	35.0	35.0	37.0	30.0	37.0
7	34.52564515167769	35.0	35.0	37.0	32.0	37.0
8	34.682823767805	35.0	35.0	37.0	32.0	37.0
9	35.96511711067406	37.0	35.0	39.0	31.0	39.0
10	35.2990941057274	37.0	34.0	39.0	30.0	39.0
11	35.339394481196734	37.0	34.0	39.0	30.0	39.0
12	35.303873889981524	37.0	34.0	39.0	30.0	39.0
13	35.1791882710531	37.0	34.0	39.0	29.0	39.0
14	35.78493354788724	38.0	34.0	40.0	27.0	41.0
15	36.127880088205494	38.0	34.0	40.0	30.0	41.0
16	36.29463019250253	38.0	34.0	40.0	30.0	41.0
17	36.144072948328265	38.0	34.0	40.0	30.0	41.0
18	36.24364384051493	38.0	35.0	40.0	30.0	41.0
19	36.26819834316706	38.0	34.0	40.0	30.0	41.0
20	36.33372668216223	38.0	34.0	40.0	30.0	41.0
21	36.01860063174206	38.0	34.0	40.0	30.0	41.0
22	36.18435544430538	38.0	34.0	40.0	30.0	41.0
23	36.31169914774421	38.0	34.0	40.0	30.0	41.0
24	36.21784373323798	38.0	34.0	40.0	30.0	41.0
25	35.76736992669408	38.0	34.0	40.0	29.0	41.0
26	35.85938971333214	38.0	34.0	40.0	30.0	41.0
27	35.85320340902318	38.0	34.0	40.0	30.0	41.0
28	35.803534179629295	38.0	34.0	40.0	29.0	41.0
29	35.878032063889385	38.0	34.0	40.0	30.0	41.0
30	35.33149770546517	37.0	34.0	40.0	27.0	41.0
31	35.53994278562489	38.0	34.0	40.0	29.0	41.0
32	35.49445735741105	38.0	34.0	40.0	29.0	41.0
33	35.54022289766971	38.0	34.0	40.0	29.0	41.0
34	35.568448656058166	38.0	34.0	40.0	29.0	41.0
35	35.539918946301924	38.0	34.0	40.0	29.0	41.0
36	35.312903033553845	38.0	34.0	40.0	28.0	41.0
37	35.32917337147625	37.0	34.0	40.0	28.0	41.0
38	35.26328744263663	37.0	34.0	40.0	28.0	41.0
39	35.25480064366172	37.0	34.0	40.0	28.0	41.0
40	35.22037070147208	37.0	34.0	40.0	28.0	41.0
41	35.09837296620776	37.0	33.0	40.0	27.0	41.0
42	35.31871386852613	37.0	34.0	40.0	28.0	41.0
43	35.283997854460935	37.0	34.0	40.0	28.0	41.0
44	35.18783002562727	37.0	34.0	40.0	28.0	41.0
45	35.3079742535312	37.0	34.0	40.0	28.0	41.0
46	35.22138387269801	37.0	34.0	40.0	28.0	41.0
47	35.15632636033137	37.0	33.0	40.0	27.0	41.0
48	35.11317122593719	37.0	33.0	40.0	28.0	41.0
49	35.189623934680256	37.0	34.0	40.0	28.0	41.0
50	34.99548244829847	37.0	33.0	40.0	27.0	41.0
51	33.83536563561595	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	0.0
12	2.0
13	1.0
14	3.0
15	8.0
16	8.0
17	15.0
18	72.0
19	110.0
20	172.0
21	328.0
22	419.0
23	713.0
24	972.0
25	1398.0
26	1971.0
27	2490.0
28	3333.0
29	4183.0
30	5626.0
31	7184.0
32	9040.0
33	11929.0
34	16618.0
35	16771.0
36	19784.0
37	24902.0
38	28384.0
39	11350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.098396805530722	4.437093986530782	56.19405208892068	18.27045711901782
2	18.198343167054055	3.785088503486501	57.029620358781806	20.986947970677633
3	19.93980570951785	4.074140294415638	55.378151260504204	20.607902735562313
4	20.65975326300733	4.088443888193575	49.373025806067105	25.878777042731986
5	17.657786518862864	4.956791227129149	52.59729423684367	24.78812801716431
6	19.831336790035163	5.961022706955123	54.88586924131354	19.32177126169617
7	79.94934143870313	1.885094463317242	12.650336730436857	5.515227367542762
8	80.61803444782169	2.563323201621074	9.347398533881638	7.471243816675606
9	74.5694022289767	4.739257405089695	13.077656594552714	7.6136837713808925
10	38.99874843554443	21.451814768460576	21.606770367721555	17.942666428273437
11	27.54335776863937	26.443768996960486	27.4682639013052	18.54460933309494
12	25.588533285654684	22.242684307765657	33.59020203826211	18.57858036831754
13	21.707491507241194	28.47547529650158	31.074557482567496	18.742475713689732
14	17.434888849156685	30.280112044817926	31.588890875499136	20.696108230526253
15	15.263722510280708	27.22331485785804	38.31813576494428	19.19482686691698
16	16.863341081113294	25.7732880386197	33.81250372489421	23.55086715537279
17	16.4217176232195	24.98420644853686	30.951188986232793	27.642886942010847
18	18.168544013350022	23.136062935812625	35.25120686572502	23.444186185112343
19	20.74259491030455	25.418082126467606	31.47327015912748	22.366052804100363
20	22.129447523690327	26.391918469515467	31.826688122057334	19.651945884736875
21	21.39579235949699	26.751296263186124	31.91072173550271	19.942189641814174
22	18.883723702246858	25.988437928362835	29.92609809881399	25.201740270576316
23	18.98384885869241	25.961618690029205	28.92365456821026	26.13087788306812
24	20.713391739674595	24.18618511234281	31.326062339829548	23.774360808153048
25	18.095834078312176	25.770308123249297	30.167471243816674	25.966386554621852
26	18.76691101972704	28.41826092138983	29.99165623696287	22.82317182192026
27	17.983789260385006	29.930269980332557	30.477978425412715	21.607962333869718
28	16.326956314440668	26.086179152512067	35.4770844508016	22.109780082245663
29	18.01537636331128	27.958161988199535	31.70629954109303	22.32016210739615
30	19.514869777698312	30.445795339412363	29.141188390249717	20.898146492639608
31	21.762321950056617	29.80272960247929	28.06543894153406	20.36950950593003
32	21.357649442755825	30.364741641337385	27.614279754454973	20.663329161451816
33	22.57703081232493	29.63347040944037	26.60885630848084	21.18064246975386
34	19.878419452887538	25.382323142022766	31.802252816020026	22.93700458906967
35	20.48036235770904	27.170272364264854	28.54997318076167	23.799392097264437
36	24.268430776565946	27.222718874783958	29.218666189880203	19.29018415876989
37	19.646582037070146	28.91888670361762	32.696823410215146	18.737707849097085
38	17.979021395792362	31.700339710352228	29.29197210799213	21.02866678586328
39	21.62762977531438	28.019548244829846	29.883187317480182	20.469634662375586
40	24.575958042791584	25.380535192800522	28.448060075093867	21.595446689314024
41	21.14607545145718	24.75236903271947	28.574408486798973	25.527147029024377
42	23.87091006615412	23.618809225817987	29.2127063591394	23.297574348888492
43	23.431074557482567	24.98420644853686	29.09887359198999	22.485845401990584
44	18.738303832171166	26.30073305918112	29.924906132665836	25.03605697598188
45	20.356397878300257	27.486739376601705	26.657130937481377	25.499731807616666
46	22.86727456940223	26.484891829072055	30.399904642708147	20.24792895881757
47	19.540497049883783	25.356099886763218	33.146790631146075	21.956612432206928
48	19.406400858215626	24.69217474223732	32.91912509684725	22.982299302699804
49	22.0376661302819	22.754037785326897	33.78568448656058	21.42261159783062
50	21.883902497169082	22.64616484891829	31.144883485309016	24.325049168603613
51	20.25865665415102	22.61100184754753	28.105369807497464	29.02497169080398
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	71.0
1	146.0
2	221.0
3	377.5
4	534.0
5	385.0
6	236.0
7	269.5
8	303.0
9	333.5
10	364.0
11	382.0
12	400.0
13	406.0
14	412.0
15	402.0
16	392.0
17	448.5
18	505.0
19	474.0
20	443.0
21	552.0
22	661.0
23	743.0
24	825.0
25	998.0
26	1476.0
27	1781.0
28	2070.5
29	2360.0
30	2693.5
31	3027.0
32	3238.5
33	3450.0
34	3806.5
35	4163.0
36	4956.0
37	5749.0
38	6674.0
39	7599.0
40	9252.0
41	10905.0
42	12770.5
43	14636.0
44	17369.0
45	20102.0
46	21849.0
47	23596.0
48	22370.0
49	21144.0
50	16680.0
51	12216.0
52	10383.5
53	8551.0
54	7113.0
55	5675.0
56	5104.0
57	4533.0
58	4113.5
59	3694.0
60	3155.0
61	2616.0
62	2208.5
63	1801.0
64	1557.5
65	1314.0
66	1097.5
67	881.0
68	687.5
69	494.0
70	463.5
71	433.0
72	355.5
73	278.0
74	217.0
75	93.0
76	30.0
77	41.5
78	53.0
79	31.0
80	9.0
81	6.0
82	3.0
83	2.5
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	167790.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.74408486798975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.05477238971258	33.87567793074677
2	10.88933475779764	9.962453066332916
3	4.1170492743049225	5.649919542285
4	2.257862782395706	4.1313546695273855
5	1.5999166167235586	3.6593360748554744
6	1.209057508403471	3.3184337564813164
7	0.9628162701618157	3.083020442219441
8	0.870312947859395	3.18493354788724
9	0.6983349401985565	2.875022349365278
>10	3.232404825807124	22.04660587639311
>50	0.07165750319201605	2.2426843077656593
>100	0.031268728665607	2.7713212944752366
>500	0.0013028636944002918	0.5679718695989034
>1k	0.003908591083200875	2.6312652720662735
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1955	1.165146909827761	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	1292	0.7700101317122594	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1168	0.6961082305262531	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	953	0.5679718695989034	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	357	0.2127659574468085	No Hit
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	356	0.2121699743727278	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	315	0.18773466833541927	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC	305	0.1817748375946123	No Hit
TTCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	304	0.18117885452053162	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	275	0.16389534537219141	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	239	0.14243995470528636	No Hit
TGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	222	0.13230824244591452	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	187	0.11144883485309016	No Hit
TCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	172	0.10250908874187972	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8540437451576375	0.0
2	0.0	0.0	0.0	3.6188092258179867	0.0
3	0.0	0.0	0.0	4.613504976458668	0.0
4	0.0	0.0	0.0	6.055188032659872	0.0
5	0.0	0.0	0.0	8.19476726860957	0.0
6	0.0	0.0	0.0	9.337862804696346	0.0
7	0.0	0.0	0.0	10.433279694856665	0.0
8	0.0	0.0	0.0	12.114547946838309	0.0
9	0.0	0.0	0.0	12.801716431253352	0.0
10	0.0	0.0	0.0	14.303593777936706	0.0
11	0.0	0.0	0.0	16.881220573335717	0.0
12	0.0	0.0	0.0	19.15847189939806	0.0
13	0.0	0.0	0.0	19.941593658740093	0.0
14	0.0	0.0	0.0	20.21991775433578	0.0
15	0.0	0.0	0.0	20.638893855414505	0.0
16	0.0	0.0	0.0	21.829668037427737	0.0
17	0.0	0.0	0.0	23.6492043625961	0.0
18	0.0	0.0	0.0	25.995589725251804	0.0
19	0.0	0.0	0.0	27.232850587043327	0.0
20	0.0	0.0	0.0	28.413493056797186	0.0
21	0.0	0.0	0.0	29.920734251147266	0.0
22	0.0	0.0	0.0	31.355265510459503	0.0
23	0.0	0.0	0.0	32.72543059777102	0.0
24	0.0	0.0	0.0	33.85362655700578	0.0
25	0.0	0.0	0.0	34.77799630490494	0.0
26	0.0	0.0	0.0	35.64574766076643	0.0
27	0.0	0.0	0.0	36.461648489182906	0.0
28	0.0	0.0	0.0	37.30556052208117	0.0
29	0.0	0.0	0.0	38.20251504857262	0.0
30	0.0	0.0	0.0	39.171583527027835	0.0
31	0.0	0.0	0.0	40.09416532570475	0.0
32	0.0	0.0	0.0	40.96430061386257	0.0
33	0.0	0.0	0.0	41.785565289945765	0.0
34	0.0	0.0	0.0	42.60325406758448	0.0
35	0.0	0.0	0.0	43.45014601585315	0.0
36	0.0	0.0	0.0	44.23326777519519	0.0
37	0.0	0.0	0.0	45.04082484057453	0.0
38	0.0	0.0	0.0	45.85553370284284	0.0
39	0.0	0.0	0.0	46.664282734370346	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAC	20	7.0160505E-4	45.000004	39
CTGTCGG	20	7.0160505E-4	45.000004	2
TCACCGA	20	7.0160505E-4	45.000004	13
CTACTGG	35	1.2036435E-7	45.000004	2
GGATGCA	40	6.7557266E-9	45.000004	8
TTTCGCG	20	7.0160505E-4	45.000004	1
ACCGAGT	20	7.0160505E-4	45.000004	15
GCCATTC	20	7.0160505E-4	45.000004	35
AGGCGCA	20	7.0160505E-4	45.000004	23
GCGCATA	20	7.0160505E-4	45.000004	25
ACAGGCG	20	7.0160505E-4	45.000004	33
ATCCCTA	20	7.0160505E-4	45.000004	21
CGGGCAC	35	1.2036435E-7	45.000004	6
TAGGGAT	20	7.0160505E-4	45.000004	5
AGTTCGT	20	7.0160505E-4	45.000004	19
TTCGGTG	20	7.0160505E-4	45.000004	1
CGATGTG	20	7.0160505E-4	45.000004	10
TAGGCGC	20	7.0160505E-4	45.000004	22
CACACCG	20	7.0160505E-4	45.000004	17
GGCGCAT	20	7.0160505E-4	45.000004	24
>>END_MODULE