SRR3549629_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549629_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	211832
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	1648	0.7779749990558557	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	1354	0.6391857698553571	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	1331	0.628328109067563	Illumina PCR Primer Index 5 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	1314	0.6203028815287587	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	369	0.17419464481287056	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	317	0.14964688998829262	Illumina PCR Primer Index 5 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	300	0.1416216624494883	No Hit
TGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG	297	0.1402054458249934	Illumina PCR Primer Index 5 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG	264	0.12462706295554968	Illumina PCR Primer Index 5 (95% over 22bp)
TTTGTTGGGACAATAGGAATTGAACCTACACTTAAGAATTCAAAATTCTCC	224	0.10574417462895125	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	40	6.7702786E-9	45.000004	1
GGTACGG	20	7.020245E-4	45.000004	8
ACTGCGT	20	7.020245E-4	45.000004	34
GCGCGAC	40	6.7702786E-9	45.000004	9
GACCGTT	20	7.020245E-4	45.000004	1
CGCGACC	40	6.7702786E-9	45.000004	10
ACGGGCG	40	6.7702786E-9	45.000004	5
TCAGGAG	20	7.020245E-4	45.000004	11
AAAGTAG	20	7.020245E-4	45.000004	26
GCCGATG	20	7.020245E-4	45.000004	9
TCTCCCG	20	7.020245E-4	45.000004	1
ACCGTTC	20	7.020245E-4	45.000004	2
TCATACG	20	7.020245E-4	45.000004	19
GGCGCGA	40	6.7702786E-9	45.000004	8
TAGCTTG	20	7.020245E-4	45.000004	1
GATTAAC	20	7.020245E-4	45.000004	9
GTACGGC	20	7.020245E-4	45.000004	9
GTCGAGG	25	3.8800004E-5	45.0	10
TTAGCGG	25	3.8800004E-5	45.0	2
GGGTCGA	25	3.8800004E-5	45.0	8