##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549629_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	211832
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.853105290985308	31.0	30.0	31.0	25.0	34.0
2	30.249216360134447	31.0	30.0	33.0	27.0	34.0
3	30.27074757354885	31.0	30.0	33.0	26.0	34.0
4	33.87683164016768	35.0	33.0	37.0	28.0	37.0
5	34.30316949280562	35.0	35.0	37.0	32.0	37.0
6	33.89004494127422	35.0	35.0	37.0	30.0	37.0
7	34.37277653989954	35.0	35.0	37.0	32.0	37.0
8	34.50760508327354	35.0	35.0	37.0	32.0	37.0
9	35.67677687979153	37.0	35.0	39.0	30.0	39.0
10	35.12030288152876	37.0	34.0	39.0	30.0	39.0
11	35.30280599720533	37.0	34.0	39.0	30.0	39.0
12	35.296399977340535	37.0	34.0	39.0	30.0	39.0
13	35.200569319083044	37.0	34.0	39.0	29.0	39.0
14	35.7565854073039	38.0	34.0	40.0	27.0	41.0
15	36.10542788624948	38.0	34.0	40.0	30.0	41.0
16	36.26279315684127	38.0	34.0	40.0	30.0	41.0
17	36.09376770270781	38.0	34.0	40.0	30.0	41.0
18	36.227614335888816	38.0	35.0	40.0	30.0	41.0
19	36.20263227463273	38.0	34.0	40.0	30.0	41.0
20	36.282020657879826	38.0	34.0	40.0	30.0	41.0
21	35.927730465652026	38.0	34.0	40.0	29.0	41.0
22	36.09620359530194	38.0	34.0	40.0	30.0	41.0
23	36.228034480154086	38.0	34.0	40.0	30.0	41.0
24	36.110738698591334	38.0	34.0	40.0	30.0	41.0
25	35.61153650062314	38.0	34.0	40.0	29.0	41.0
26	35.681969674081344	38.0	34.0	40.0	29.0	41.0
27	35.77498678197817	38.0	34.0	40.0	29.0	41.0
28	35.6428018429699	38.0	34.0	40.0	29.0	41.0
29	35.83716813323766	38.0	34.0	40.0	29.0	41.0
30	35.33677631330488	38.0	34.0	40.0	27.0	41.0
31	35.41436138071679	38.0	34.0	40.0	28.0	41.0
32	35.56878092072964	38.0	34.0	40.0	29.0	41.0
33	35.619099097397935	38.0	34.0	40.0	29.0	41.0
34	35.59955058725782	38.0	34.0	40.0	29.0	41.0
35	35.64012519354961	38.0	34.0	40.0	29.0	41.0
36	35.4260404471468	38.0	34.0	40.0	28.0	41.0
37	35.42163129272254	38.0	34.0	40.0	28.0	41.0
38	35.33011537444767	38.0	34.0	40.0	28.0	41.0
39	35.31622228936138	37.0	34.0	40.0	28.0	41.0
40	35.19207201933608	37.0	34.0	40.0	27.0	41.0
41	35.05341497035386	37.0	33.0	40.0	27.0	41.0
42	35.3578684995657	37.0	34.0	40.0	28.0	41.0
43	35.3335048529023	37.0	34.0	40.0	28.0	41.0
44	35.18239925979078	37.0	34.0	40.0	27.0	41.0
45	35.306587295592735	37.0	34.0	40.0	28.0	41.0
46	35.1594471090298	37.0	34.0	40.0	27.0	41.0
47	35.09921069526795	37.0	33.0	40.0	27.0	41.0
48	35.13130688470108	37.0	34.0	40.0	28.0	41.0
49	35.182087692133386	37.0	34.0	40.0	28.0	41.0
50	34.99300861059708	37.0	34.0	40.0	27.0	41.0
51	33.859275274746025	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	1.0
12	6.0
13	8.0
14	8.0
15	9.0
16	18.0
17	56.0
18	107.0
19	160.0
20	258.0
21	463.0
22	669.0
23	962.0
24	1357.0
25	1953.0
26	2542.0
27	3321.0
28	4269.0
29	5430.0
30	7003.0
31	8979.0
32	11302.0
33	14853.0
34	20040.0
35	21369.0
36	25184.0
37	31644.0
38	35679.0
39	14177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.988500321009102	4.266116545186752	55.127176252879636	17.618206880924507
2	20.319404056044412	3.693020884474489	54.47477246119566	21.512802598285433
3	22.80344801540844	3.5065523622493293	53.20442614902375	20.485573473318478
4	20.616337474980174	5.449129498848144	46.66905849918804	27.265474526983645
5	17.71214925034933	5.563370973224065	50.591506476830695	26.132973299595907
6	21.601552173420448	6.021281015144076	53.12181351259488	19.255353298840593
7	79.42237244608935	2.871615242267457	12.091185467729144	5.614826843914045
8	79.74149325880887	3.0037954605536465	9.657181162430605	7.5975301182068815
9	72.95451112202123	6.306412628875712	13.36200385210922	7.377072396993844
10	36.60589523773557	25.912987650591035	21.60768911212659	15.873427999546811
11	28.97484799274897	26.02581290834246	26.30716794440878	18.692171154499793
12	26.233524679935044	21.917368480682807	32.71554817024812	19.133558669134032
13	22.900694890290417	26.656501378450848	31.95503984289437	18.487763888364363
14	17.152743683673854	29.57154726386948	31.935684882359606	21.340024170097056
15	15.526926998753728	27.147928547150574	37.88851542731976	19.436629026775936
16	17.43362664753201	25.248782053702932	34.7058045998716	22.611786698893464
17	16.91717965179954	24.903697269534348	30.646455681861095	27.532667396805017
18	19.14960912421164	23.354828354545113	34.17472336568601	23.320839155557234
19	20.52286717776351	25.684032629631027	30.678556592016314	23.114543600589148
20	23.944918614751312	25.741153366818985	30.34102496317837	19.972903055251333
21	22.288417236300464	26.602213074511877	30.122455530797993	20.986914158389666
22	20.10508327353752	25.55846142225915	28.968711054042828	25.367744250160506
23	19.083046942860378	25.901657917595077	29.10891649986782	25.906378639676724
24	21.353714264133842	24.380641262887572	30.49256014199932	23.773084330979266
25	19.776993088862874	25.667510102345254	28.6160731145436	25.93942369424827
26	19.94741115601042	28.148249556252125	28.130782884550023	23.77355640318743
27	19.056610899203143	27.780033233883454	28.909702028022206	24.253653838891196
28	16.913875146342384	28.11803693492957	31.761490237546735	23.20659768118131
29	19.284149703538652	24.679935042864155	31.72136409985271	24.314551153744475
30	21.75025491899241	25.967748026738168	28.67791457381321	23.60408248045621
31	20.645133879678234	30.43071868272971	26.66027795611617	22.263869481475886
32	20.808470863703313	28.35360096680388	28.88704256203029	21.950885607462517
33	20.76267985951131	27.02802220627667	29.224102118660074	22.985195815551947
34	19.172268590203558	26.69851580497753	30.493976358623815	23.6352392461951
35	19.05897126024397	24.68323954832131	30.76164130065335	25.496147890781373
36	22.644831753465013	25.519279428981456	29.558801314249028	22.277087503304504
37	18.74032251973262	28.69254881226632	31.162902677593564	21.404225990407493
38	18.3942935911477	28.798292986895273	29.728275236980252	23.079138184976774
39	21.333887231390914	24.928245024358926	32.23686695116885	21.50100079308131
40	23.326504022055214	24.865459420672988	30.48217455341969	21.32586200385211
41	18.255976434155368	25.586785754749048	29.963839268854564	26.19339854224102
42	21.045451112202123	23.61730050228483	30.298066392235356	25.039181993277694
43	21.632708939159333	23.71549152158314	30.718682729710334	23.93311680954719
44	19.716567846217757	25.408342460062695	29.259979606480606	25.615110087238946
45	21.43160617848106	24.524623286377885	27.839986404320406	26.20378413082065
46	22.469220892027643	24.982533328297897	30.096963631557085	22.451282148117375
47	17.44070773065448	25.931870538917636	33.26315193171948	23.36426979870841
48	18.052513312436268	23.274104006948903	33.75410702821103	24.91927565240379
49	20.783923108878735	21.781411684731296	34.659069451263264	22.775595755126705
50	20.41051399222025	22.191642433626647	31.651025340836135	25.746818233316972
51	18.62891347860569	22.7486876392613	28.646757808074323	29.97564107405869
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	169.0
1	283.0
2	397.0
3	559.5
4	722.0
5	563.5
6	405.0
7	451.5
8	498.0
9	541.0
10	584.0
11	616.0
12	648.0
13	616.0
14	584.0
15	561.0
16	538.0
17	610.5
18	683.0
19	683.5
20	684.0
21	683.0
22	682.0
23	718.5
24	755.0
25	969.5
26	1587.0
27	1990.0
28	2557.0
29	3124.0
30	3414.5
31	3705.0
32	4542.5
33	5380.0
34	5237.5
35	5095.0
36	5625.5
37	6156.0
38	6998.0
39	7840.0
40	9669.0
41	11498.0
42	13725.0
43	15952.0
44	19255.5
45	22559.0
46	25635.5
47	28712.0
48	26928.5
49	25145.0
50	21079.5
51	17014.0
52	14422.0
53	11830.0
54	10281.0
55	8732.0
56	7935.0
57	7138.0
58	6450.5
59	5763.0
60	5391.5
61	5020.0
62	4385.5
63	3751.0
64	3173.5
65	2596.0
66	2081.0
67	1566.0
68	1275.5
69	985.0
70	849.5
71	714.0
72	574.5
73	435.0
74	362.0
75	232.0
76	175.0
77	116.0
78	57.0
79	54.5
80	52.0
81	35.5
82	19.0
83	12.0
84	5.0
85	3.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	211832.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.57825068922542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.34528915292647	33.14182937422108
2	10.746470968220182	9.581177536916046
3	3.8747868814266497	5.181936629026776
4	2.13806906630238	3.812455153140224
5	1.5111562940136185	3.368235205256996
6	1.2008768307017823	3.211979304354394
7	0.97531530959113	3.043449526039503
8	0.7719922483082886	2.753125118018052
9	0.7073948173798859	2.838098115487745
>10	3.6058074149378916	24.97261981192643
>50	0.07836409653609513	2.4311718720495485
>100	0.04024102254556237	2.9981305940556666
>500	0.0	0.0
>1k	0.004235897110059197	2.665791759507534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	1648	0.7779749990558557	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	1354	0.6391857698553571	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	1331	0.628328109067563	Illumina PCR Primer Index 5 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	1314	0.6203028815287587	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	369	0.17419464481287056	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	317	0.14964688998829262	Illumina PCR Primer Index 5 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	300	0.1416216624494883	No Hit
TGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG	297	0.1402054458249934	Illumina PCR Primer Index 5 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG	264	0.12462706295554968	Illumina PCR Primer Index 5 (95% over 22bp)
TTTGTTGGGACAATAGGAATTGAACCTACACTTAAGAATTCAAAATTCTCC	224	0.10574417462895125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7057479512066166	0.0
2	0.0	0.0	0.0	3.0226783488802447	0.0
3	0.0	0.0	0.0	3.876656973450659	0.0
4	0.0	0.0	0.0	5.123399675214321	0.0
5	0.0	0.0	0.0	7.06456059518864	0.0
6	0.0	0.0	0.0	8.136636579931267	0.0
7	0.0	0.0	0.0	9.111937762000075	0.0
8	0.0	0.0	0.0	10.782129234487707	0.0
9	0.0	0.0	0.0	11.48079610257185	0.0
10	0.0	0.0	0.0	12.902205521356546	0.0
11	0.0	0.0	0.0	15.246516107103742	0.0
12	0.0	0.0	0.0	17.369896899429737	0.0
13	0.0	0.0	0.0	18.135125948865138	0.0
14	0.0	0.0	0.0	18.40184674647834	0.0
15	0.0	0.0	0.0	18.849843272026888	0.0
16	0.0	0.0	0.0	19.8992597907776	0.0
17	0.0	0.0	0.0	21.516579175950753	0.0
18	0.0	0.0	0.0	23.54507345443559	0.0
19	0.0	0.0	0.0	24.622342233468032	0.0
20	0.0	0.0	0.0	25.782695721137504	0.0
21	0.0	0.0	0.0	27.159730352354696	0.0
22	0.0	0.0	0.0	28.486725329506402	0.0
23	0.0	0.0	0.0	29.73488424789456	0.0
24	0.0	0.0	0.0	30.77533139469013	0.0
25	0.0	0.0	0.0	31.585407303901206	0.0
26	0.0	0.0	0.0	32.375184108161186	0.0
27	4.7207220816496094E-4	0.0	0.0	33.083764492616794	0.0
28	4.7207220816496094E-4	0.0	0.0	33.93821518939537	0.0
29	4.7207220816496094E-4	0.0	0.0	34.72799199365535	0.0
30	4.7207220816496094E-4	0.0	0.0	35.68677064843838	0.0
31	4.7207220816496094E-4	0.0	0.0	36.53177990105367	0.0
32	4.7207220816496094E-4	0.0	0.0	37.34940896559538	0.0
33	4.7207220816496094E-4	0.0	0.0	38.10330828203482	0.0
34	4.7207220816496094E-4	0.0	0.0	38.96153555647872	0.0
35	4.7207220816496094E-4	0.0	0.0	39.771611465689794	0.0
36	4.7207220816496094E-4	0.0	0.0	40.55572340345179	0.0
37	4.7207220816496094E-4	0.0	0.0	41.32614524717701	0.0
38	4.7207220816496094E-4	0.0	0.0	42.10978511273084	0.0
39	4.7207220816496094E-4	0.0	0.0	42.88398353412138	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	40	6.7702786E-9	45.000004	1
GGTACGG	20	7.020245E-4	45.000004	8
ACTGCGT	20	7.020245E-4	45.000004	34
GCGCGAC	40	6.7702786E-9	45.000004	9
GACCGTT	20	7.020245E-4	45.000004	1
CGCGACC	40	6.7702786E-9	45.000004	10
ACGGGCG	40	6.7702786E-9	45.000004	5
TCAGGAG	20	7.020245E-4	45.000004	11
AAAGTAG	20	7.020245E-4	45.000004	26
GCCGATG	20	7.020245E-4	45.000004	9
TCTCCCG	20	7.020245E-4	45.000004	1
ACCGTTC	20	7.020245E-4	45.000004	2
TCATACG	20	7.020245E-4	45.000004	19
GGCGCGA	40	6.7702786E-9	45.000004	8
TAGCTTG	20	7.020245E-4	45.000004	1
GATTAAC	20	7.020245E-4	45.000004	9
GTACGGC	20	7.020245E-4	45.000004	9
GTCGAGG	25	3.8800004E-5	45.0	10
TTAGCGG	25	3.8800004E-5	45.0	2
GGGTCGA	25	3.8800004E-5	45.0	8
>>END_MODULE