SRR3549628_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549628_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	238993
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC	2869	1.200453569769826	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCG	2652	1.1096559313452694	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	1835	0.7678049147882993	No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	1395	0.5836991041578624	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGCTAGTT	1262	0.528048938671844	No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	1044	0.43683287795040027	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	984	0.4117275401371588	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTA	705	0.29498771930558637	No Hit
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	566	0.2368270200382438	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC	561	0.234734908553807	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT	512	0.21423221600632655	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT	486	0.20335323628725527	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	316	0.13222144581640466	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCC	260	0.1087897971907127	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCGATCGAATGC	260	0.1087897971907127	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	257	0.10753453030005063	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGC	20	7.0220605E-4	45.000004	13
AACGTGT	20	7.0220605E-4	45.000004	19
CAAACCG	20	7.0220605E-4	45.000004	14
CCGATCG	45	3.8198777E-10	45.000004	40
TTTGTCG	40	6.7757355E-9	45.000004	1
GACCCAC	35	1.2065357E-7	45.000004	12
GCCGATG	35	1.2065357E-7	45.000004	9
CATAGTC	20	7.0220605E-4	45.000004	11
TAACAGT	20	7.0220605E-4	45.000004	40
ACGTGTC	20	7.0220605E-4	45.000004	20
GGAACCG	20	7.0220605E-4	45.000004	9
TTGCCCG	45	3.8198777E-10	45.000004	1
CGATCGA	45	3.8198777E-10	45.000004	41
CCACGCA	35	1.2065357E-7	45.000004	41
CGTCATA	40	6.7757355E-9	45.000004	38
GCCGTTT	20	7.0220605E-4	45.000004	9
CCCATAG	20	7.0220605E-4	45.000004	9
ACGACGA	20	7.0220605E-4	45.000004	34
TGATCCG	55	1.8189894E-12	45.0	4
TCTAACG	25	3.8815037E-5	45.0	16