##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549628_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	238993
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.85937245023913	31.0	30.0	31.0	25.0	34.0
2	29.705606440355993	31.0	30.0	33.0	25.0	34.0
3	29.77148703100091	31.0	30.0	33.0	25.0	34.0
4	33.84280711150536	35.0	33.0	37.0	28.0	37.0
5	34.39453038373509	35.0	35.0	37.0	32.0	37.0
6	33.55770252685225	35.0	33.0	37.0	28.0	37.0
7	34.3621193926182	35.0	35.0	37.0	32.0	37.0
8	33.991506027373184	35.0	35.0	37.0	29.0	37.0
9	35.6482993225743	37.0	35.0	39.0	30.0	39.0
10	35.22235379278891	37.0	34.0	39.0	30.0	39.0
11	35.41526320854586	37.0	35.0	39.0	30.0	39.0
12	35.42495805316474	37.0	35.0	39.0	30.0	39.0
13	35.266689819367095	37.0	34.0	39.0	30.0	39.0
14	35.96041306649149	38.0	34.0	40.0	29.0	41.0
15	36.14596243404618	38.0	34.0	40.0	30.0	41.0
16	36.4363349554171	38.0	35.0	40.0	31.0	41.0
17	36.124681476026495	38.0	34.0	40.0	30.0	41.0
18	36.28538074336905	38.0	35.0	40.0	30.0	41.0
19	36.27581560966221	38.0	34.0	40.0	30.0	41.0
20	36.12492834518166	38.0	34.0	40.0	30.0	41.0
21	36.00290803496337	38.0	34.0	40.0	30.0	41.0
22	36.17208453804086	38.0	34.0	40.0	30.0	41.0
23	36.10899063989322	38.0	34.0	40.0	30.0	41.0
24	36.038762641583645	38.0	34.0	40.0	30.0	41.0
25	35.694384354353474	38.0	34.0	40.0	29.0	41.0
26	35.75799709614926	38.0	34.0	40.0	29.0	41.0
27	35.656567347160795	38.0	34.0	40.0	29.0	41.0
28	35.556003732326886	38.0	34.0	40.0	28.0	41.0
29	35.74614737670141	38.0	34.0	40.0	29.0	41.0
30	35.33495541710427	38.0	34.0	40.0	27.0	41.0
31	35.4916922252953	38.0	34.0	40.0	28.0	41.0
32	35.36523245450704	38.0	34.0	40.0	27.0	41.0
33	35.299410442983685	38.0	34.0	40.0	27.0	41.0
34	35.23540438422883	38.0	34.0	40.0	27.0	41.0
35	35.278585565267605	38.0	34.0	40.0	27.0	41.0
36	34.99086165703598	38.0	33.0	40.0	26.0	41.0
37	35.15731841518371	38.0	34.0	40.0	27.0	41.0
38	35.00525538404891	37.0	33.0	40.0	26.0	41.0
39	34.95813684919642	37.0	33.0	40.0	26.0	41.0
40	34.94914495403631	37.0	33.0	40.0	26.0	41.0
41	34.676563748729045	37.0	33.0	40.0	25.0	41.0
42	34.975844480800696	37.0	33.0	40.0	26.0	41.0
43	34.82494466365124	37.0	33.0	40.0	26.0	41.0
44	34.92279271777834	37.0	33.0	40.0	26.0	41.0
45	35.04378370914629	37.0	34.0	40.0	27.0	41.0
46	34.894344185812976	37.0	34.0	40.0	26.0	41.0
47	34.711071035553346	37.0	33.0	40.0	26.0	41.0
48	34.717037737506956	37.0	33.0	40.0	26.0	41.0
49	34.81548831974158	37.0	33.0	40.0	27.0	41.0
50	34.51213633872122	37.0	33.0	40.0	26.0	41.0
51	33.51174302176214	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	4.0
13	3.0
14	8.0
15	13.0
16	17.0
17	40.0
18	107.0
19	208.0
20	480.0
21	876.0
22	1342.0
23	1818.0
24	2337.0
25	2730.0
26	3083.0
27	3786.0
28	4695.0
29	6143.0
30	7937.0
31	10158.0
32	12716.0
33	16448.0
34	22202.0
35	23212.0
36	27250.0
37	33902.0
38	39373.0
39	18101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.694116564083465	4.043214654822526	50.213604582561	18.04906419853301
2	18.38296519144912	11.448452465134961	50.25335470076529	19.91522764265062
3	19.84744323055487	11.439665596900328	48.41480712824225	20.298084044302552
4	18.78841639713297	4.640303272480784	50.6140347206822	25.95724560970405
5	23.36135367981489	5.257894582686522	45.871636407760896	25.509115329737693
6	20.472147719807694	12.920043683287796	49.44956546844468	17.15824312845983
7	77.00895005293043	2.5787366157167786	15.95360533572113	4.458707995631672
8	77.23573493784338	8.852142112948915	7.821986418012243	6.0901365311954745
9	71.47615202118891	5.425263501441465	10.278125300741026	12.820459176628605
10	40.64177611896583	25.774395065964274	17.714326361023126	15.869502454046772
11	32.389651579753384	24.383140928813816	25.25011192796442	17.97709556346839
12	26.35474679174704	20.79851711137983	29.780370136363825	23.0663659605093
13	25.57397078575523	23.691488872059015	32.11600339758905	18.618536944596702
14	19.880917014305858	29.842715058600046	28.86528057307118	21.41108735402292
15	16.69630491269619	23.780194398999132	37.04962070018787	22.473879988116806
16	18.13191181331671	22.788115133079213	31.826455168143	27.253517885461083
17	19.356215454009114	25.008682262660415	30.678722807780982	24.956379475549493
18	20.05958333507676	21.899804596787355	31.79298138439201	26.24763068374388
19	21.437447958726825	24.736289347386744	28.861096350102304	24.96516634378413
20	25.704518542384086	24.067650516960747	31.54443854004092	18.683392400614242
21	23.73333110174775	28.861096350102304	27.715037678927835	19.690534869222112
22	21.21191834070454	23.523701531007184	30.689183365203164	24.57519676308511
23	22.550032846150305	26.334244099199562	27.52925817910985	23.58646487554029
24	21.26380270551857	25.194880184775286	29.022607356700824	24.518709753005318
25	19.311025845945277	29.11005761675028	27.812948496399475	23.765968040904966
26	19.091354140079417	25.319570029247718	31.079152945902184	24.50992288477068
27	21.890180883958944	27.063972584971108	27.574447787173682	23.471398743896266
28	18.157435573426834	27.112509571410044	32.081692769244285	22.648362085918837
29	20.24494441259786	22.36048754566033	31.916834384270672	25.47773365747114
30	20.140757260672906	27.589929412158515	29.40002426849322	22.86928905867536
31	23.053394869305794	26.133819818990517	25.16559062399317	25.647194687710517
32	24.595281033335702	26.630487085395803	26.412070646420606	22.362161234847882
33	22.424087734787214	25.0588929382869	27.279041645571212	25.237977681354685
34	21.352508232458693	25.36517805960844	28.544769093655464	24.737544614277407
35	22.616143569058508	23.329135162954564	27.48867121631178	26.566050051675155
36	22.245421414016313	25.442167762235712	30.092513169841794	22.21989765390618
37	20.282602419317723	25.818329407137448	28.253965597318746	25.64510257622608
38	22.763428217562858	25.07228245178729	26.888653642575306	25.275635688074544
39	20.521103128543512	26.102438146723962	29.640617089203445	23.735841635529074
40	21.422803178335766	24.124974371634316	30.390011422928705	24.062211027101213
41	19.531952818701804	25.461415187892534	28.940178164214014	26.066453829191648
42	23.239174369123784	22.174708045842348	29.74187528505019	24.844242299983684
43	21.381379370943918	23.221600632654514	30.406329892507312	24.990690103894256
44	20.28469453080216	23.022431619336132	30.176197629219264	26.516676220642445
45	21.29100015481625	22.489780035398528	28.115467817049034	28.103751992736186
46	23.13122141652684	25.140066863883042	28.366939617478337	23.36177210211178
47	17.637336658395853	25.369780704874202	33.54826291983447	23.444619716895474
48	20.189712669408728	24.698212918369993	29.51257986635592	25.599494545865358
49	21.844154431301334	21.108149611076474	33.57085772386639	23.4768382337558
50	21.650843330139377	22.279313620064187	30.18791345353211	25.881929596264325
51	19.72861129823886	22.94795245049018	28.40083182352621	28.922604427744748
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	81.0
1	174.5
2	268.0
3	442.5
4	617.0
5	479.0
6	341.0
7	361.5
8	382.0
9	441.5
10	501.0
11	540.5
12	580.0
13	552.5
14	525.0
15	543.5
16	562.0
17	587.5
18	613.0
19	634.5
20	656.0
21	784.5
22	913.0
23	990.5
24	1068.0
25	1370.5
26	2073.5
27	2474.0
28	2943.0
29	3412.0
30	3689.0
31	3966.0
32	4261.0
33	4556.0
34	5281.0
35	6006.0
36	6313.0
37	6620.0
38	7218.5
39	7817.0
40	9416.5
41	11016.0
42	12623.0
43	14230.0
44	15488.0
45	16746.0
46	22982.5
47	29219.0
48	27519.5
49	25820.0
50	24521.5
51	23223.0
52	19969.5
53	16716.0
54	14847.5
55	12979.0
56	11889.0
57	10799.0
58	9969.5
59	9140.0
60	8316.0
61	7492.0
62	6689.0
63	5886.0
64	5143.0
65	4400.0
66	3422.0
67	2444.0
68	2168.0
69	1892.0
70	1582.0
71	1272.0
72	1101.0
73	930.0
74	746.0
75	462.0
76	362.0
77	242.5
78	123.0
79	100.5
80	78.0
81	51.5
82	25.0
83	14.5
84	4.0
85	3.5
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	238993.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.00368211621261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.71706796644561	29.889578355851427
2	9.297533627596595	7.438711594063424
3	3.3627596594356004	4.035683053478554
4	1.98732297136163	3.1800094563439094
5	1.4570215258456582	2.914311297820438
6	1.3043114448883961	3.1306356253112018
7	1.0522352153630525	2.9465298146807646
8	0.9204443235780182	2.94569297008699
9	0.8482731209338327	3.0540643449808154
>10	4.936928644645733	30.37620348713142
>50	0.07426312156140828	1.9766269304958721
>100	0.030332824299730145	2.0929483290305573
>500	0.005229797293056921	1.3925094040411226
>1k	0.006275756751668306	4.626495336683501
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC	2869	1.200453569769826	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCG	2652	1.1096559313452694	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	1835	0.7678049147882993	No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	1395	0.5836991041578624	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGCTAGTT	1262	0.528048938671844	No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	1044	0.43683287795040027	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	984	0.4117275401371588	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTA	705	0.29498771930558637	No Hit
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	566	0.2368270200382438	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC	561	0.234734908553807	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT	512	0.21423221600632655	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT	486	0.20335323628725527	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	316	0.13222144581640466	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCC	260	0.1087897971907127	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCGATCGAATGC	260	0.1087897971907127	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	257	0.10753453030005063	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44603816848192207	0.0
2	0.0	0.0	0.0	1.8490081299452286	0.0
3	0.0	0.0	0.0	2.24609088969133	0.0
4	0.0	0.0	0.0	3.0800065273878316	0.0
5	0.0	0.0	0.0	4.90516458641048	0.0
6	0.0	0.0	0.0	5.481750511521258	0.0
7	0.0	0.0	0.0	6.243697514153134	0.0
8	0.0	0.0	0.0	7.384316695468068	0.0
9	0.0	0.0	0.0	7.768009941713774	0.0
10	0.0	0.0	0.0	9.765557987054015	0.0
11	0.0	0.0	0.0	11.1526279012356	0.0
12	0.0	0.0	0.0	13.946433577552481	0.0
13	0.0	0.0	0.0	14.515487901319286	0.0
14	0.0	0.0	0.0	14.777420259170771	0.0
15	0.0	0.0	0.0	15.375345721422804	0.0
16	0.0	0.0	0.0	16.12432163285117	0.0
17	0.0	0.0	0.0	17.074558669082357	0.0
18	0.0	0.0	0.0	18.151159238973527	0.0
19	0.0	0.0	0.0	19.44073675798036	0.0
20	0.0	0.0	0.0	20.21649169640952	0.0
21	0.0	0.0	0.0	21.087646918528996	0.0
22	0.0	0.0	0.0	21.981815366977276	0.0
23	0.0	0.0	0.0	22.78393091011034	0.0
24	0.0	0.0	0.0	23.458846074989644	0.0
25	0.0	0.0	0.0	24.091500587883328	0.0
26	0.0	0.0	0.0	24.627917972492916	0.0
27	0.0	0.0	0.0	25.180653826681116	0.0
28	0.0	0.0	0.0	25.764352930838978	0.0
29	0.0	0.0	0.0	26.32587565326181	0.0
30	0.0	0.0	0.0	26.986564460046946	0.0
31	0.0	0.0	0.0	27.651855912097844	0.0
32	0.0	0.0	0.0	28.241412928412128	0.0
33	0.0	0.0	0.0	28.84435945822681	0.0
34	0.0	0.0	0.0	29.455674433979237	0.0
35	0.0	0.0	0.0	30.09125790295113	0.0
36	0.0	0.0	0.0	30.74567037528296	0.0
37	0.0	0.0	0.0	31.339830036863006	0.0
38	0.0	0.0	0.0	31.974158238944238	0.0
39	0.0	0.0	0.0	32.66497345110526	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGC	20	7.0220605E-4	45.000004	13
AACGTGT	20	7.0220605E-4	45.000004	19
CAAACCG	20	7.0220605E-4	45.000004	14
CCGATCG	45	3.8198777E-10	45.000004	40
TTTGTCG	40	6.7757355E-9	45.000004	1
GACCCAC	35	1.2065357E-7	45.000004	12
GCCGATG	35	1.2065357E-7	45.000004	9
CATAGTC	20	7.0220605E-4	45.000004	11
TAACAGT	20	7.0220605E-4	45.000004	40
ACGTGTC	20	7.0220605E-4	45.000004	20
GGAACCG	20	7.0220605E-4	45.000004	9
TTGCCCG	45	3.8198777E-10	45.000004	1
CGATCGA	45	3.8198777E-10	45.000004	41
CCACGCA	35	1.2065357E-7	45.000004	41
CGTCATA	40	6.7757355E-9	45.000004	38
GCCGTTT	20	7.0220605E-4	45.000004	9
CCCATAG	20	7.0220605E-4	45.000004	9
ACGACGA	20	7.0220605E-4	45.000004	34
TGATCCG	55	1.8189894E-12	45.0	4
TCTAACG	25	3.8815037E-5	45.0	16
>>END_MODULE