FastQCFastQC Report
Sat 18 Jun 2016
SRR3549620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013015
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC261932.5856477939615896No Hit
GAATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC231972.2898969906664757No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG215362.125931007931768No Hit
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC75110.7414500278870501TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC60010.5923900435827703TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCTTCCCT56520.5579384313164168No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT56280.5555692660029713No Hit
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT55650.5493502070551768TruSeq Adapter, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT53520.5283238648983479No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTC52510.5183536275375982No Hit
TCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC46360.45764376638055704TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTA42360.4181576778231319No Hit
TCCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG19220.18973065551842766No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGT15750.1554764736948614No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC15580.1537983149311708No Hit
CGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG14810.14619724288386649No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATG12350.12191329842105003No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12160.12003770921457234No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG11850.1169775373513719No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTAT11310.11164691539611951No Hit
TGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG11260.11115333928915169No Hit
GAATGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT10460.10325612157766667No Hit
TTCCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT10360.10226896936373103No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGT207.032892E-445.021
CGGCCAT207.032892E-445.041
CGTTCCA207.032892E-445.014
TTATCCG207.032892E-445.030
GCCGCAC551.8189894E-1245.017
CGCTCTA207.032892E-445.012
TCCACCG207.032892E-445.021
TCGTACA207.032892E-445.018
TTACGAA207.032892E-445.018
CGACTTA207.032892E-445.026
CGGAGAT253.8904775E-545.06
CGGATAT302.1652468E-644.99999624
CTACGGC3100.044.2741936
GGCGACT5650.043.4070810
TACGGCT30800.042.9545487
CGAGGGA1000.042.754
TTTCTCG1950.042.6923071
TCGTTTG3600.042.51
CGTTTGG6250.042.4800032
CGATGAA6450.042.209319