##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549620_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1013015
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.293024288880225	33.0	31.0	34.0	30.0	34.0
2	32.495793250840315	34.0	31.0	34.0	31.0	34.0
3	32.584447416869445	34.0	31.0	34.0	31.0	34.0
4	36.01610736267479	37.0	35.0	37.0	35.0	37.0
5	36.03967167317364	37.0	35.0	37.0	35.0	37.0
6	35.35137584339817	37.0	35.0	37.0	33.0	37.0
7	35.88081222884163	37.0	35.0	37.0	35.0	37.0
8	35.771838521640845	37.0	35.0	37.0	35.0	37.0
9	37.770033020241556	39.0	38.0	39.0	35.0	39.0
10	37.492357961135816	39.0	37.0	39.0	35.0	39.0
11	37.42556131942765	39.0	37.0	39.0	35.0	39.0
12	37.34521206497436	39.0	37.0	39.0	35.0	39.0
13	37.254660592390046	39.0	37.0	39.0	34.0	39.0
14	38.4912444534385	40.0	38.0	41.0	34.0	41.0
15	38.42096908732842	40.0	38.0	41.0	34.0	41.0
16	38.6006515204612	40.0	38.0	41.0	35.0	41.0
17	38.45289753853596	40.0	38.0	41.0	34.0	41.0
18	38.45385803764012	40.0	38.0	41.0	34.0	41.0
19	38.49690083562435	40.0	38.0	41.0	34.0	41.0
20	38.33845204661333	40.0	38.0	41.0	34.0	41.0
21	38.39703854335819	40.0	38.0	41.0	34.0	41.0
22	38.409878432204856	40.0	38.0	41.0	34.0	41.0
23	38.295477362131855	40.0	37.0	41.0	34.0	41.0
24	38.25049579719945	40.0	37.0	41.0	34.0	41.0
25	38.12188072239799	40.0	37.0	41.0	34.0	41.0
26	37.96024145743153	40.0	37.0	41.0	33.0	41.0
27	37.89851482949413	40.0	37.0	41.0	33.0	41.0
28	37.64615627606699	40.0	36.0	41.0	33.0	41.0
29	37.526121528309055	40.0	36.0	41.0	32.0	41.0
30	37.61551408419421	40.0	36.0	41.0	33.0	41.0
31	37.490600830195014	40.0	36.0	41.0	32.0	41.0
32	37.38645133586373	39.0	36.0	41.0	32.0	41.0
33	37.37129854937982	39.0	36.0	41.0	32.0	41.0
34	37.11442574887835	40.0	36.0	41.0	31.0	41.0
35	37.15931649580707	39.0	36.0	41.0	31.0	41.0
36	36.849433621417255	39.0	35.0	41.0	30.0	41.0
37	37.01738078903077	39.0	35.0	41.0	31.0	41.0
38	37.0286323499652	39.0	35.0	41.0	31.0	41.0
39	37.13281540747175	39.0	35.0	41.0	31.0	41.0
40	37.14229897879103	39.0	36.0	41.0	32.0	41.0
41	37.040967804030544	39.0	36.0	41.0	31.0	41.0
42	37.05534370172209	39.0	36.0	41.0	31.0	41.0
43	36.90933204345444	39.0	35.0	41.0	31.0	41.0
44	36.887835816843776	39.0	35.0	41.0	31.0	41.0
45	36.88641727911235	39.0	35.0	41.0	31.0	41.0
46	36.796502519706024	39.0	35.0	41.0	31.0	41.0
47	36.62920785970593	39.0	35.0	41.0	31.0	41.0
48	36.60998208318732	39.0	35.0	41.0	31.0	41.0
49	36.78249877839914	39.0	35.0	41.0	31.0	41.0
50	36.76567770467367	39.0	35.0	41.0	31.0	41.0
51	35.85840880934636	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	5.0
11	9.0
12	6.0
13	6.0
14	14.0
15	10.0
16	20.0
17	53.0
18	101.0
19	268.0
20	571.0
21	1218.0
22	1999.0
23	2841.0
24	3633.0
25	4352.0
26	5259.0
27	6230.0
28	7683.0
29	10088.0
30	13354.0
31	18133.0
32	24511.0
33	33297.0
34	53508.0
35	72691.0
36	77724.0
37	127417.0
38	242720.0
39	305090.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.72578885801296	3.739332586388158	49.76668657423631	14.768191981362566
2	16.495510925307126	16.17991836251191	47.71696371722038	19.607606994960587
3	17.66232484217904	16.394722684264302	47.07837495002542	18.86457752353124
4	17.22847144415433	4.027482317635968	57.35611022541621	21.387936012793492
5	25.693301678652343	4.3610410507248165	45.79112846305336	24.154528807569484
6	18.54977468250717	14.153492297744851	49.434213708582796	17.862519311165183
7	74.88842712101993	1.6675962350014562	18.308613396642695	5.135363247335923
8	74.17767752698627	10.197084939512248	9.247345794484781	6.377891739016698
9	70.27319437520669	4.215830960054886	9.928875682985938	15.582098981752491
10	37.332517287503144	33.7954521897504	15.970543377936162	12.901487144810295
11	27.09890771607528	22.293154592972463	31.15886734154973	19.449070349402525
12	23.03756607750132	20.034747757930536	33.55863437362724	23.369051790940905
13	22.578935158906827	21.75387333849943	37.69776360665933	17.969427895934416
14	19.20119642848329	28.575292567237405	29.40144025508013	22.82207074919917
15	14.62643692344141	24.413360118063405	40.7514202652478	20.208782693247386
16	16.789484854617157	26.84846719939981	29.958391534182617	26.40365641180042
17	17.12225386593486	26.111558071696866	33.33060221220811	23.435585850160166
18	17.20487850624127	25.43289092461612	32.553713419840776	24.80851714930184
19	18.565075541823173	26.67196438354812	30.48780126651629	24.27515880811242
20	22.481601950612777	26.71993998114539	34.189523353553504	16.60893471468833
21	22.304506843432723	30.612281160693573	29.115560973924374	17.96765102194933
22	18.78886294872238	23.306762486241567	32.17415339358252	25.730221171453532
23	19.43446049663628	29.0905860229118	30.099751731218195	21.375201749233725
24	22.41417945440097	22.776365601693954	28.551403483660167	26.258051460244914
25	17.49480511147416	32.10436173205727	26.387763261156056	24.01306989531251
26	17.495397402802524	26.831883042205696	30.0888930568649	25.58382649812688
27	20.037018208022587	28.589803704782256	29.159587962665906	22.21359012452925
28	14.524760245406041	26.92970982660671	33.83108838467347	24.71444154331377
29	18.468235909636086	25.736835091286903	31.7834385473068	24.01149045177021
30	17.977325113645897	26.097836655923164	34.86690720275613	21.05793102767481
31	19.70967853388153	24.273875510234298	26.976698271990053	29.039747683894117
32	19.536532035557222	28.30333213229814	29.817623628475392	22.342512203669244
33	17.827080546684897	22.20026356964112	29.721376287616668	30.251279596057312
34	19.088661076094628	24.813255479928728	27.72969798078015	28.3683854631965
35	15.772619359042068	22.7740951516019	31.095887030300638	30.357398459055396
36	16.336085842756525	26.882918811666162	27.864937834089325	28.916057511487985
37	15.395329782875871	23.184553042156335	37.32047403049313	24.099643144474662
38	16.959571181078267	22.82759880159721	29.777742679032393	30.435087338292128
39	18.728646663672304	22.263540026554395	33.244818684817105	25.762994624956193
40	17.8914428710335	21.239468319817576	34.32199918066367	26.547089628485267
41	16.039150456804688	24.048804805456978	26.386085102392364	33.52595963534597
42	17.7334985168038	21.79799904246235	30.51998242869059	29.948520012043257
43	20.295454657630934	21.91675345379881	27.497124919176912	30.29066696939335
44	18.95075591180782	21.334432362798182	31.833388449331945	27.88142327606205
45	18.207035433828718	19.76476162741914	29.661752293894956	32.36645064485719
46	23.135491577123734	23.361352003672206	29.347245598535064	24.155910820668993
47	14.434435817830932	21.15546166641165	38.70100640168211	25.709096114075308
48	17.42126227153596	22.376667670271416	30.53321026835733	29.668859789835295
49	17.39194385078207	17.95521290405374	37.63231541487539	27.020527830288792
50	19.35667290217815	18.438621343218017	32.020651224315536	30.184054530288297
51	16.97319388163058	18.851349683864505	28.69572513733755	35.47973129716737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78.0
1	372.5
2	667.0
3	1567.0
4	2467.0
5	1741.0
6	1015.0
7	1033.0
8	1051.0
9	1065.5
10	1080.0
11	1125.5
12	1171.0
13	1388.5
14	1606.0
15	1531.5
16	1457.0
17	1618.5
18	1780.0
19	2079.0
20	2378.0
21	2725.5
22	3073.0
23	4054.5
24	5036.0
25	5982.5
26	9201.5
27	11474.0
28	12991.0
29	14508.0
30	16471.0
31	18434.0
32	21136.5
33	23839.0
34	25994.0
35	28149.0
36	31583.0
37	35017.0
38	38648.5
39	42280.0
40	45876.5
41	49473.0
42	54911.5
43	60350.0
44	67154.5
45	73959.0
46	128328.0
47	182697.0
48	153425.0
49	124153.0
50	113140.0
51	102127.0
52	82406.5
53	62686.0
54	52529.0
55	42372.0
56	36886.0
57	31400.0
58	28911.5
59	26423.0
60	24153.0
61	21883.0
62	17532.0
63	13181.0
64	10780.5
65	8380.0
66	6341.5
67	4303.0
68	3339.5
69	2376.0
70	1900.5
71	1425.0
72	1274.0
73	1123.0
74	859.5
75	440.5
76	285.0
77	263.0
78	241.0
79	145.5
80	50.0
81	34.0
82	18.0
83	19.5
84	21.0
85	11.5
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1013015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.73354639975804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.21952035930167	17.040630997358118
2	13.786441577925443	7.0954806806619555
3	6.000836969956265	4.63268449811259
4	2.9947674130237547	3.0826394471812053
5	1.7879644598554352	2.300533319440408
6	1.1081604249842487	1.7110138628824652
7	0.8213666113333632	1.4795673062771388
8	0.5811826492758191	1.1964712537498876
9	0.45301010102395967	1.049180080883323
>10	5.735036084167797	35.64890136777657
>50	0.41751157984785786	6.625399892866279
>100	0.08024595162966214	3.802017990042407
>500	0.005039600826983583	0.8411685477549714
>1k	0.005039600826983583	2.332666526583788
>5k	0.002713631214529622	4.086131844881835
>10k+	0.0011629848062269807	7.075512383547094
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC	26193	2.5856477939615896	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	23197	2.2898969906664757	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	21536	2.125931007931768	No Hit
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	7511	0.7414500278870501	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	6001	0.5923900435827703	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCTTCCCT	5652	0.5579384313164168	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	5628	0.5555692660029713	No Hit
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT	5565	0.5493502070551768	TruSeq Adapter, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT	5352	0.5283238648983479	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTC	5251	0.5183536275375982	No Hit
TCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	4636	0.45764376638055704	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTA	4236	0.4181576778231319	No Hit
TCCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG	1922	0.18973065551842766	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGT	1575	0.1554764736948614	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC	1558	0.1537983149311708	No Hit
CGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG	1481	0.14619724288386649	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATG	1235	0.12191329842105003	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1216	0.12003770921457234	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	1185	0.1169775373513719	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTAT	1131	0.11164691539611951	No Hit
TGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG	1126	0.11115333928915169	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT	1046	0.10325612157766667	No Hit
TTCCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT	1036	0.10226896936373103	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9614566418068836E-4	0.0	0.0	0.5769904690453744	0.0
2	2.9614566418068836E-4	0.0	0.0	2.5115126626950244	0.0
3	2.9614566418068836E-4	0.0	0.0	3.4323282478541777	0.0
4	2.9614566418068836E-4	0.0	0.0	4.881072837026105	0.0
5	2.9614566418068836E-4	0.0	0.0	8.666209286140877	0.0
6	2.9614566418068836E-4	0.0	0.0	9.534607088740048	0.0
7	2.9614566418068836E-4	0.0	0.0	10.914349738157876	0.0
8	2.9614566418068836E-4	0.0	0.0	12.569112994378168	0.0
9	2.9614566418068836E-4	0.0	0.0	13.090526793778967	0.0
10	2.9614566418068836E-4	0.0	0.0	16.483763813961293	0.0
11	2.9614566418068836E-4	0.0	0.0	18.52815604902198	0.0
12	2.9614566418068836E-4	0.0	0.0	23.35888412313737	0.0
13	2.9614566418068836E-4	0.0	0.0	24.124124519380267	0.0
14	3.948608855742511E-4	0.0	0.0	24.466567622394535	0.0
15	3.948608855742511E-4	0.0	0.0	25.252636930351475	0.0
16	3.948608855742511E-4	0.0	0.0	26.14364051864977	0.0
17	3.948608855742511E-4	0.0	0.0	27.293574132663387	0.0
18	3.948608855742511E-4	0.0	0.0	28.410142001845976	0.0
19	3.948608855742511E-4	0.0	0.0	30.039535446168124	0.0
20	3.948608855742511E-4	0.0	0.0	31.043370532519262	0.0
21	3.948608855742511E-4	0.0	0.0	32.235159400403745	0.0
22	5.922913283613767E-4	0.0	0.0	33.63059777002315	0.0
23	5.922913283613767E-4	0.0	0.0	34.853580647868	0.0
24	5.922913283613767E-4	0.0	0.0	35.74823669935786	0.0
25	5.922913283613767E-4	0.0	0.0	36.49481991875737	0.0
26	5.922913283613767E-4	0.0	0.0	37.19382240144519	0.0
27	5.922913283613767E-4	0.0	0.0	37.91829341125255	0.0
28	5.922913283613767E-4	0.0	0.0	38.59172865159943	0.0
29	5.922913283613767E-4	0.0	0.0	39.342556625518874	0.0
30	5.922913283613767E-4	0.0	0.0	40.1916062447249	0.0
31	5.922913283613767E-4	0.0	0.0	40.97441795037586	0.0
32	5.922913283613767E-4	0.0	0.0	41.7129065216211	0.0
33	5.922913283613767E-4	0.0	0.0	42.42414969176172	0.0
34	5.922913283613767E-4	0.0	0.0	43.11841384382265	0.0
35	5.922913283613767E-4	0.0	0.0	43.856408838960924	0.0
36	5.922913283613767E-4	0.0	0.0	44.54336806463873	0.0
37	5.922913283613767E-4	0.0	0.0	45.2783028879138	0.0
38	5.922913283613767E-4	0.0	0.0	45.96160965040004	0.0
39	5.922913283613767E-4	0.0	0.0	46.66426459627942	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGGT	20	7.032892E-4	45.0	21
CGGCCAT	20	7.032892E-4	45.0	41
CGTTCCA	20	7.032892E-4	45.0	14
TTATCCG	20	7.032892E-4	45.0	30
GCCGCAC	55	1.8189894E-12	45.0	17
CGCTCTA	20	7.032892E-4	45.0	12
TCCACCG	20	7.032892E-4	45.0	21
TCGTACA	20	7.032892E-4	45.0	18
TTACGAA	20	7.032892E-4	45.0	18
CGACTTA	20	7.032892E-4	45.0	26
CGGAGAT	25	3.8904775E-5	45.0	6
CGGATAT	30	2.1652468E-6	44.999996	24
CTACGGC	310	0.0	44.274193	6
GGCGACT	565	0.0	43.40708	10
TACGGCT	3080	0.0	42.954548	7
CGAGGGA	100	0.0	42.75	4
TTTCTCG	195	0.0	42.692307	1
TCGTTTG	360	0.0	42.5	1
CGTTTGG	625	0.0	42.480003	2
CGATGAA	645	0.0	42.2093	19
>>END_MODULE