FastQCFastQC Report
Sat 18 Jun 2016
SRR3549617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2083686
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC93970.4509796581634661No Hit
TCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC61960.29735766329475743No Hit
CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT51640.24783004732958805No Hit
GCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC34930.16763562264179918No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34780.16691574450277058No Hit
CGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG26830.12876220313425343No Hit
TCCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG24970.11983571421029847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG700.045.0000041
ACGGGAT3700.039.5270275
GCTACGA2050.039.51219610
CGTTTGG12250.039.48982
TACCCGC403.460027E-739.37500428
TTTCGCG8250.039.2727281
TACCGGG1500.039.0000043
TTTTCCG13400.038.9552231
TACGGGA5200.038.9423074
TTCGTTG6850.038.7591251
TGGGCGA22700.038.6563876
TTGGGAC57200.038.627625
TTTGGGA162400.038.6268464
CGAACGA356.2507333E-638.5714322
TGCGACG356.2507333E-638.571431
TTGTTCG5100.038.3823551
CGTTTTT22350.038.255031
CGAGATA4400.037.84090819
TCGTTTG7500.037.81
TTGGGAT107550.037.782435
Produced by FastQC (version 0.11.5)