##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549617_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2083686
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.487352700934785	34.0	31.0	34.0	31.0	34.0
2	32.72312238984185	34.0	31.0	34.0	31.0	34.0
3	32.76860860993451	34.0	31.0	34.0	31.0	34.0
4	36.12046488770381	37.0	35.0	37.0	35.0	37.0
5	36.152385724144615	37.0	35.0	37.0	35.0	37.0
6	35.81226777931032	37.0	35.0	37.0	35.0	37.0
7	36.13194262475248	37.0	35.0	37.0	35.0	37.0
8	36.14513607136584	37.0	37.0	37.0	35.0	37.0
9	37.985683543489756	39.0	39.0	39.0	37.0	39.0
10	37.60186899561642	39.0	37.0	39.0	35.0	39.0
11	37.532678148243065	39.0	37.0	39.0	35.0	39.0
12	37.473962007711336	39.0	37.0	39.0	35.0	39.0
13	37.4806132017972	39.0	37.0	39.0	35.0	39.0
14	38.754109784295714	40.0	38.0	41.0	35.0	41.0
15	38.79612907127081	40.0	38.0	41.0	35.0	41.0
16	38.84763827179335	40.0	38.0	41.0	35.0	41.0
17	38.82204660395088	40.0	38.0	41.0	35.0	41.0
18	38.77599647931598	40.0	38.0	41.0	35.0	41.0
19	38.794067340280634	40.0	38.0	41.0	35.0	41.0
20	38.75132145630388	40.0	38.0	41.0	35.0	41.0
21	38.67203791742134	40.0	38.0	41.0	35.0	41.0
22	38.6579115087398	40.0	38.0	41.0	35.0	41.0
23	38.600472432026706	40.0	38.0	41.0	35.0	41.0
24	38.57705335640783	40.0	38.0	41.0	35.0	41.0
25	38.47874007887945	40.0	38.0	41.0	34.0	41.0
26	38.42152416438945	40.0	38.0	41.0	34.0	41.0
27	38.41719481726133	40.0	38.0	41.0	34.0	41.0
28	38.35877718619792	40.0	38.0	41.0	34.0	41.0
29	38.3588400555554	40.0	38.0	41.0	34.0	41.0
30	38.30847402151764	40.0	38.0	41.0	34.0	41.0
31	38.26294700833043	40.0	38.0	41.0	34.0	41.0
32	38.17866511556924	40.0	38.0	41.0	34.0	41.0
33	38.11543197967448	40.0	37.0	41.0	34.0	41.0
34	38.104263310306834	40.0	37.0	41.0	34.0	41.0
35	38.06599458843607	40.0	37.0	41.0	34.0	41.0
36	37.96880432080457	40.0	37.0	41.0	34.0	41.0
37	37.916019976138436	40.0	37.0	41.0	33.0	41.0
38	37.88863533181103	40.0	37.0	41.0	33.0	41.0
39	37.86086867215118	40.0	37.0	41.0	33.0	41.0
40	37.771611461611776	40.0	37.0	41.0	33.0	41.0
41	37.65325581685532	40.0	37.0	41.0	33.0	41.0
42	37.59762891337754	40.0	36.0	41.0	33.0	41.0
43	37.49194552346179	40.0	36.0	41.0	33.0	41.0
44	37.41263462920997	40.0	36.0	41.0	33.0	41.0
45	37.43387295398635	40.0	36.0	41.0	33.0	41.0
46	37.34916585320437	40.0	36.0	41.0	33.0	41.0
47	37.28018281065381	39.0	36.0	41.0	32.0	41.0
48	37.21700726501018	39.0	35.0	41.0	32.0	41.0
49	37.18869877707102	39.0	35.0	41.0	32.0	41.0
50	37.12369138152294	39.0	35.0	41.0	32.0	41.0
51	36.23707794744506	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	30.0
10	40.0
11	44.0
12	14.0
13	17.0
14	21.0
15	35.0
16	66.0
17	107.0
18	191.0
19	441.0
20	711.0
21	1263.0
22	1984.0
23	3040.0
24	4361.0
25	6648.0
26	9378.0
27	11906.0
28	14236.0
29	17267.0
30	21204.0
31	28032.0
32	36754.0
33	52359.0
34	93565.0
35	124135.0
36	136736.0
37	222021.0
38	456427.0
39	840143.0
40	504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.036985419108255	4.538831666575483	58.312192911983864	18.111990002332405
2	18.569112620615584	4.296952611861864	55.496989469622584	21.63694529789997
3	19.337942473098156	4.40138293389695	53.543288192174835	22.717386400830065
4	18.969028922783952	4.413284919128889	52.133478844701166	24.48420731338599
5	18.960630344495282	5.444582341101299	50.32936824454356	25.265419069859856
6	21.70091846852165	5.746115297602422	53.21603159017242	19.33693464370351
7	85.74343735092523	1.7590462286544133	8.266120711086028	4.231395709334325
8	87.60729783662221	2.284797229524986	5.941634200162596	4.166270733690201
9	81.57692665785535	5.2811220116658655	8.208050541204386	4.933900789274392
10	35.09372333451393	38.809158385668475	14.139702431172452	11.957415848645141
11	25.274297566907872	25.317058328366176	30.413123666425747	18.995520438300204
12	25.910525866181374	21.875752872553733	31.559505606890863	20.654215654374028
13	24.296223135347645	21.846573811985106	32.77797134501071	21.07923170765653
14	19.60367349015159	25.41462581214252	31.609465149739453	23.37223554796644
15	17.01379190530627	27.16700117004194	34.75552458479829	21.06368233985351
16	20.284198290913313	26.185423331538438	32.65256857319193	20.877809804356318
17	19.941920231743172	26.119962412762764	30.299382920459223	23.638734435034834
18	20.14665357448291	26.2029403662548	31.06202182094615	22.588384238316138
19	21.92720016355631	27.19642018999024	30.054432385685754	20.821947260767697
20	25.323201288485887	26.652624243768013	29.55075764774539	18.47341682000071
21	25.086889291380754	25.96077336028557	29.402654718609234	19.54968262972444
22	22.82416832478598	25.043120700527815	29.598557556176893	22.534153418509316
23	21.79733414727555	26.335685895091675	29.317277171320438	22.549702786312334
24	22.18885187115525	24.350070020146987	30.065854452158337	23.39522365653942
25	21.227622588048295	26.572621786583966	27.613757543123103	24.585998082244636
26	21.302057987623854	28.339538682891764	27.870898014384128	22.487505315100258
27	20.94878978886454	27.513982433053734	29.1344761158831	22.40275166219862
28	18.984194355579486	27.066458189957604	30.713216866648814	23.236130587814095
29	20.44693874220972	24.37708944629853	31.025212052103818	24.15075975938793
30	21.379852818514884	24.349014198876414	30.861511763288714	23.40962121931999
31	20.319712279105392	26.83129799787492	28.848924454068413	24.00006526895127
32	20.943510682511665	27.322014929312765	28.673418163773235	23.061056224402332
33	20.646584946100326	26.62622871200363	28.879495279039162	23.847691062856878
34	20.32115203538345	24.99690452400218	30.02318967445191	24.658753766162462
35	20.677971632961974	24.468609953707038	29.35850219274881	25.49491622058218
36	20.220561063423183	26.190558462263507	28.279260886717093	25.30961958759621
37	19.46488098494687	26.51459960857826	29.525129985995967	24.4953894204789
38	18.93068341391169	25.32876834609437	29.97212631845681	25.768421921537122
39	19.96855572288723	24.396814107307915	32.03870448810425	23.595925681700603
40	20.740984966064946	23.54198281314939	33.67076421303402	22.04626800775165
41	18.428496424125324	25.41764930032644	31.064373422866975	25.089480852681255
42	20.07231415865922	26.19175825916189	27.824825813486292	25.911101768692596
43	20.85107832946039	25.628813554441503	28.77357720885009	24.746530907248022
44	20.461096345610617	24.48540711028437	29.444167691293217	25.609328852811796
45	21.27326286206271	22.368149519649315	29.018959670506977	27.339627947780997
46	20.36151320304499	23.500373856713537	30.28536929268613	25.852743647555343
47	18.37436158807037	24.06725389526061	31.98241001763222	25.575974499036803
48	18.39495010284659	22.990604150529396	32.09087165724586	26.523574089378148
49	19.3198975277465	22.53501727227615	32.921802997188635	25.22328220278871
50	18.783924257301724	22.53300161348687	31.645603032318686	27.037471096892716
51	17.539446922424972	22.104098218253615	30.120565190724513	30.2358896685969
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	282.0
1	1382.5
2	2483.0
3	4750.0
4	7017.0
5	4912.5
6	2808.0
7	2771.0
8	2734.0
9	2834.0
10	2934.0
11	2912.0
12	2890.0
13	3043.5
14	3197.0
15	3311.0
16	3425.0
17	3649.0
18	3873.0
19	4369.0
20	4865.0
21	6079.0
22	7293.0
23	9035.0
24	10777.0
25	13243.5
26	20763.5
27	25817.0
28	31053.0
29	36289.0
30	41755.5
31	47222.0
32	52312.0
33	57402.0
34	64473.0
35	71544.0
36	76563.5
37	81583.0
38	87479.0
39	93375.0
40	101719.0
41	110063.0
42	117144.0
43	124225.0
44	134533.5
45	144842.0
46	161003.5
47	177165.0
48	196661.5
49	216158.0
50	210187.0
51	204216.0
52	177383.0
53	150550.0
54	129817.0
55	109084.0
56	99557.0
57	90030.0
58	85988.5
59	81947.0
60	76828.5
61	71710.0
62	59098.5
63	46487.0
64	38109.0
65	29731.0
66	23885.0
67	18039.0
68	14291.0
69	10543.0
70	8592.0
71	6641.0
72	5520.0
73	4399.0
74	3219.5
75	1604.5
76	1169.0
77	906.0
78	643.0
79	468.5
80	294.0
81	217.5
82	141.0
83	82.0
84	23.0
85	16.5
86	10.0
87	9.5
88	9.0
89	6.0
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2083686.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.722758115607476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.84240739358113	17.862134846435612
2	13.318659199205415	7.118226164091533
3	5.407949716859184	4.335459965549878
4	2.837826958467758	3.033382535403358
5	1.64289681427136	2.195136708833783
6	1.0470605023460646	1.67882067219602
7	0.7422668135575238	1.3884791561158292
8	0.5541939755967826	1.1847673247199757
9	0.44395865913752963	1.0677419875315473
>10	6.508694619388655	44.283221086287455
>50	0.5677944782186954	9.244832888426574
>100	0.0795975632592945	3.784990662832778
>500	0.00416070379851847	0.7367730082375272
>1k	0.0019899018166827467	1.0826081962784213
>5k	5.427004954589308E-4	1.0034247970596648
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	9397	0.4509796581634661	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	6196	0.29735766329475743	No Hit
CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT	5164	0.24783004732958805	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	3493	0.16763562264179918	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3478	0.16691574450277058	No Hit
CGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG	2683	0.12876220313425343	No Hit
TCCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG	2497	0.11983571421029847	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.598375187048336E-5	0.0	0.0	0.25858022753908216	0.0
2	9.598375187048336E-5	0.0	0.0	1.2416458141965727	0.0
3	9.598375187048336E-5	0.0	0.0	1.9020140270654984	0.0
4	9.598375187048336E-5	0.0	0.0	2.5713087288583787	0.0
5	9.598375187048336E-5	0.0	0.0	3.601310370180536	0.0
6	9.598375187048336E-5	0.0	0.0	4.274060487040754	0.0
7	9.598375187048336E-5	0.0	0.0	4.894307491627817	0.0
8	9.598375187048336E-5	0.0	0.0	5.896281877403793	0.0
9	9.598375187048336E-5	0.0	0.0	6.355708105731861	0.0
10	9.598375187048336E-5	0.0	0.0	7.23995841983869	0.0
11	9.598375187048336E-5	0.0	0.0	9.154738285902962	0.0
12	9.598375187048336E-5	0.0	0.0	11.044610368356844	0.0
13	9.598375187048336E-5	0.0	0.0	11.589318160221838	0.0
14	9.598375187048336E-5	0.0	0.0	11.77327102068162	0.0
15	9.598375187048336E-5	0.0	0.0	12.056423088699544	0.0
16	9.598375187048336E-5	0.0	0.0	12.697210616186892	0.0
17	9.598375187048336E-5	0.0	0.0	13.654408581715288	0.0
18	9.598375187048336E-5	0.0	0.0	14.608583059059763	0.0
19	9.598375187048336E-5	0.0	0.0	15.404240370190134	0.0
20	1.4397562780572504E-4	0.0	0.0	16.249233329781934	0.0
21	1.4397562780572504E-4	0.0	0.0	17.454933228903013	0.0
22	1.4397562780572504E-4	0.0	0.0	18.77115841830295	0.0
23	1.4397562780572504E-4	0.0	0.0	19.94335998802123	0.0
24	1.4397562780572504E-4	0.0	0.0	20.803086453525147	0.0
25	1.4397562780572504E-4	0.0	0.0	21.583050421224694	0.0
26	1.4397562780572504E-4	0.0	0.0	22.272645686538183	0.0
27	1.4397562780572504E-4	0.0	0.0	22.940740591432682	0.0
28	1.4397562780572504E-4	0.0	0.0	23.65092437152239	0.0
29	1.9196750374096672E-4	0.0	0.0	24.388079585887702	0.0
30	1.9196750374096672E-4	0.0	0.0	25.261099801025683	0.0
31	1.9196750374096672E-4	0.0	0.0	26.06645147109497	0.0
32	2.399593796762084E-4	0.0	0.0	26.837009031111215	0.0
33	2.399593796762084E-4	0.0	0.0	27.553383763196567	0.0
34	2.399593796762084E-4	0.0	0.0	28.26308762452692	0.0
35	2.399593796762084E-4	0.0	0.0	29.013872531657842	0.0
36	2.399593796762084E-4	0.0	0.0	29.7832302947757	0.0
37	2.399593796762084E-4	0.0	0.0	30.534495120665973	0.0
38	2.399593796762084E-4	0.0	0.0	31.247558413311793	0.0
39	2.399593796762084E-4	0.0	0.0	31.995319832258794	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	70	0.0	45.000004	1
ACGGGAT	370	0.0	39.527027	5
GCTACGA	205	0.0	39.512196	10
CGTTTGG	1225	0.0	39.4898	2
TACCCGC	40	3.460027E-7	39.375004	28
TTTCGCG	825	0.0	39.272728	1
TACCGGG	150	0.0	39.000004	3
TTTTCCG	1340	0.0	38.955223	1
TACGGGA	520	0.0	38.942307	4
TTCGTTG	685	0.0	38.759125	1
TGGGCGA	2270	0.0	38.656387	6
TTGGGAC	5720	0.0	38.62762	5
TTTGGGA	16240	0.0	38.626846	4
CGAACGA	35	6.2507333E-6	38.57143	22
TGCGACG	35	6.2507333E-6	38.57143	1
TTGTTCG	510	0.0	38.382355	1
CGTTTTT	2235	0.0	38.25503	1
CGAGATA	440	0.0	37.840908	19
TCGTTTG	750	0.0	37.8	1
TTGGGAT	10755	0.0	37.78243	5
>>END_MODULE