SRR3549615_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549615_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	867900
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	5185	0.5974190574951032	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	4121	0.47482428851250147	TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT	4063	0.46814149095517915	Illumina Paired End PCR Primer 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	3374	0.3887544648000922	TruSeq Adapter, Index 21 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTG	1655	0.1906901716787648	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1273	0.14667588431846987	No Hit
CGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTG	1115	0.1284710220071437	No Hit
GCCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTG	1000	0.11522064754003918	TruSeq Adapter, Index 21 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCT	915	0.10542689249913584	TruSeq Adapter, Index 21 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	20	7.0323306E-4	45.0	26
CTATCGG	45	3.8380676E-10	45.0	2
GAGTCGT	35	1.2114651E-7	45.0	9
CGTTGCG	20	7.0323306E-4	45.0	2
ATTCGAA	20	7.0323306E-4	45.0	10
TAAGTCG	20	7.0323306E-4	45.0	10
CACGACG	20	7.0323306E-4	45.0	15
CGACAGG	20	7.0323306E-4	45.0	38
CCCGTAA	20	7.0323306E-4	45.0	16
CCGTACT	20	7.0323306E-4	45.0	22
TATCTCG	20	7.0323306E-4	45.0	1
GGGCGTA	25	3.890013E-5	45.0	7
TCCGTAC	20	7.0323306E-4	45.0	21
TTCGGTA	20	7.0323306E-4	45.0	40
AACTCGA	20	7.0323306E-4	45.0	10
CCGCGCA	20	7.0323306E-4	45.0	25
CGGTAAT	20	7.0323306E-4	45.0	42
CGTTTCG	20	7.0323306E-4	45.0	13
CCGCAAT	20	7.0323306E-4	45.0	15
TGCGTCG	20	7.0323306E-4	45.0	2