##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549615_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 867900 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5024634174444 34.0 31.0 34.0 31.0 34.0 2 32.73161308906556 34.0 31.0 34.0 31.0 34.0 3 32.79701693743519 34.0 31.0 34.0 31.0 34.0 4 36.135439566770366 37.0 35.0 37.0 35.0 37.0 5 36.178297038829356 37.0 35.0 37.0 35.0 37.0 6 35.84739831777855 37.0 35.0 37.0 35.0 37.0 7 36.1636674732112 37.0 36.0 37.0 35.0 37.0 8 36.16763567231248 37.0 37.0 37.0 35.0 37.0 9 38.02734185966125 39.0 39.0 39.0 37.0 39.0 10 37.679700426316394 39.0 37.0 39.0 35.0 39.0 11 37.61210969005646 39.0 37.0 39.0 35.0 39.0 12 37.53004493605254 39.0 37.0 39.0 35.0 39.0 13 37.51506394745938 39.0 37.0 39.0 35.0 39.0 14 38.79544878442217 40.0 38.0 41.0 35.0 41.0 15 38.85396359027538 40.0 38.0 41.0 35.0 41.0 16 38.890720129047125 40.0 38.0 41.0 35.0 41.0 17 38.85336905173407 40.0 38.0 41.0 35.0 41.0 18 38.80849291393017 40.0 38.0 41.0 35.0 41.0 19 38.84880746629796 40.0 38.0 41.0 35.0 41.0 20 38.81560087567692 40.0 38.0 41.0 35.0 41.0 21 38.74730959787994 40.0 38.0 41.0 35.0 41.0 22 38.71981910358336 40.0 38.0 41.0 35.0 41.0 23 38.677533125936165 40.0 38.0 41.0 35.0 41.0 24 38.65242885125014 40.0 38.0 41.0 35.0 41.0 25 38.56396819910128 40.0 38.0 41.0 35.0 41.0 26 38.529343242309025 40.0 38.0 41.0 35.0 41.0 27 38.53865998386911 40.0 38.0 41.0 35.0 41.0 28 38.50114644544303 40.0 38.0 41.0 35.0 41.0 29 38.49983408226754 40.0 38.0 41.0 35.0 41.0 30 38.44000115220648 40.0 38.0 41.0 34.0 41.0 31 38.37410876829128 40.0 38.0 41.0 34.0 41.0 32 38.24559281023159 40.0 38.0 41.0 34.0 41.0 33 38.212794100702844 40.0 38.0 41.0 34.0 41.0 34 38.20616084802396 40.0 38.0 41.0 34.0 41.0 35 38.13171563544187 40.0 37.0 41.0 34.0 41.0 36 38.05845949994239 40.0 37.0 41.0 34.0 41.0 37 38.0109505703422 40.0 37.0 41.0 34.0 41.0 38 37.972992280216616 40.0 37.0 41.0 34.0 41.0 39 37.98081345777163 40.0 37.0 41.0 34.0 41.0 40 37.870650996658604 40.0 37.0 41.0 33.0 41.0 41 37.73124438299343 40.0 37.0 41.0 33.0 41.0 42 37.69137919115106 40.0 37.0 41.0 33.0 41.0 43 37.65372969236087 40.0 37.0 41.0 33.0 41.0 44 37.582910473556865 40.0 36.0 41.0 33.0 41.0 45 37.613549948150705 40.0 36.0 41.0 33.0 41.0 46 37.54415485655029 40.0 36.0 41.0 33.0 41.0 47 37.45322387371817 40.0 36.0 41.0 33.0 41.0 48 37.39519184237815 39.0 36.0 41.0 33.0 41.0 49 37.409935476437376 39.0 36.0 41.0 33.0 41.0 50 37.34139416983523 39.0 36.0 41.0 33.0 41.0 51 36.46122018665745 38.0 35.0 40.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 13.0 10 13.0 11 13.0 12 9.0 13 6.0 14 11.0 15 10.0 16 15.0 17 23.0 18 64.0 19 148.0 20 249.0 21 403.0 22 661.0 23 1033.0 24 1488.0 25 2260.0 26 3220.0 27 4207.0 28 5269.0 29 6348.0 30 7902.0 31 10589.0 32 14205.0 33 20854.0 34 39072.0 35 54551.0 36 55281.0 37 91393.0 38 193598.0 39 354737.0 40 251.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.771171793985484 4.391634980988593 57.231247839612855 16.605945385413065 2 17.74524714828897 4.26385528286669 56.16522640857241 21.82567116027192 3 19.94112224910704 3.9474593847217423 54.70814609978108 21.403272266390136 4 19.101163728540154 4.11775550178592 53.199446940891804 23.581633828782117 5 18.41594653761954 5.286323309136998 51.62772208779813 24.67000806544533 6 21.138264777048047 5.979375504090333 53.961977186311785 18.920382532549834 7 84.5013250374467 1.7398317778545915 9.238852402350501 4.519990782348197 8 86.22306717363752 2.467565387717479 6.600875676921304 4.7084917617237005 9 80.37435188385759 5.375388869685447 8.650190114068442 5.6000691323885246 10 34.31501325037446 39.70238506740408 14.397626454660676 11.58497522756078 11 24.46952413872566 24.505703422053234 31.461113031455234 19.563659407765872 12 24.245765641202905 22.150132503744672 32.96140108307409 20.64270077197834 13 22.652494527019243 23.754464800092176 33.84906095172255 19.74397972116603 14 19.089180781195992 26.084111072704232 32.19115105426892 22.635557091830858 15 17.065790989745363 27.320659062103932 34.618619656642466 20.994930291508236 16 19.681875792141952 26.18815531743288 32.656757690978225 21.47321119944694 17 19.954487844221685 25.8871989860583 30.061988708376543 24.096324461343475 18 19.604101855052424 25.344279294849635 32.27249683143219 22.779122018665745 19 21.427583823021084 25.93916349809886 31.089065560548452 21.544187118331607 20 23.79433114414103 26.645466067519298 30.640511579675078 18.919691208664595 21 24.63693973960134 26.291162576333676 30.159119714252796 18.912777969812193 22 21.517801590044936 24.74017743979721 30.76206936282982 22.979951607328033 23 20.512962322848256 26.291277796981223 30.313400161308905 22.88235971886162 24 21.85943081000115 24.572185735683835 31.00725890079502 22.56112455351999 25 19.686138956100933 26.73199677382187 29.216153934785115 24.365710335292086 26 18.44267772784883 27.83120175135384 30.985136536467333 22.740983984329993 27 19.069362829819102 27.310058762530243 31.62034796635557 22.00023044129508 28 16.96912086645927 25.69593271114184 33.40626800322618 23.928678419172716 29 19.057610323770017 23.608134577716328 32.20313400161309 25.131121096900564 30 21.316511118792487 24.547989399700427 31.49533356377463 22.640165917732457 31 22.282521027768176 26.104389906671276 28.26097476667819 23.35211429888236 32 21.08560894112225 26.891231708722202 29.727157506625186 22.296001843530362 33 21.52413872565964 24.899758036640165 30.6408572416177 22.9352459960825 34 20.38333909436571 23.342550985136537 31.877520451664935 24.396589468832815 35 19.470330683258442 23.181357299228022 32.50409033298767 24.844221684525866 36 20.727387947920267 24.073165111187926 33.091369973499255 22.108076967392556 37 19.67876483465837 25.622421938011293 32.38760225832469 22.311210969005646 38 19.211545108883513 25.782924300034566 29.612167300380225 25.393363290701693 39 22.04793178937666 23.79041364212467 30.09321350385989 24.068441064638783 40 23.64500518492914 23.053577601106117 31.457425970733954 21.843991243230786 41 19.804701002419634 24.255328954948727 31.91416061758267 24.02580942504897 42 19.445903905979954 23.919921649959672 32.78326996197718 23.85090448208319 43 20.33287245074317 22.91577370664823 31.79686599838691 24.954487844221685 44 19.84917617237009 23.572877059569073 30.326304873833394 26.251641894227447 45 20.79214195183777 22.141951837769327 29.317893766562968 27.748012443829932 46 21.96992741099205 22.453854130660215 31.64938356953566 23.926834888812078 47 18.743057955985712 23.03779237239313 34.10438990667127 24.114759764949877 48 18.773937089526445 21.5303606406268 33.726581403387485 25.96912086645927 49 20.035833621384953 21.03306832584399 34.430233897914505 24.50086415485655 50 20.806659753427816 20.706648231363058 31.66194262011752 26.8247493950916 51 19.397511234013134 21.095863578753313 30.420670584168686 29.085954603064867 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 96.0 1 427.5 2 759.0 3 1634.0 4 2509.0 5 1777.0 6 1045.0 7 1057.0 8 1069.0 9 1106.5 10 1144.0 11 1197.5 12 1251.0 13 1279.5 14 1308.0 15 1355.0 16 1402.0 17 1437.0 18 1472.0 19 1957.5 20 2443.0 21 2956.5 22 3470.0 23 4360.0 24 5250.0 25 5932.5 26 8954.5 27 11294.0 28 13071.0 29 14848.0 30 16734.0 31 18620.0 32 21380.5 33 24141.0 34 26254.5 35 28368.0 36 31480.5 37 34593.0 38 36695.5 39 38798.0 40 43443.0 41 48088.0 42 52828.0 43 57568.0 44 62087.5 45 66607.0 46 75098.5 47 83590.0 48 92350.0 49 101110.0 50 95948.5 51 90787.0 52 74715.5 53 58644.0 54 50414.0 55 42184.0 56 36941.5 57 31699.0 58 29335.0 59 26971.0 60 24091.0 61 21211.0 62 18093.5 63 14976.0 64 12739.0 65 10502.0 66 7983.5 67 5465.0 68 4478.5 69 3492.0 70 2813.5 71 2135.0 72 1557.5 73 980.0 74 750.5 75 473.5 76 426.0 77 346.0 78 266.0 79 184.0 80 102.0 81 77.0 82 52.0 83 34.5 84 17.0 85 12.5 86 8.0 87 5.5 88 3.0 89 1.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 867900.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.593253860673332 #Duplication Level Percentage of deduplicated Percentage of total 1 66.87321844809568 18.452496931185674 2 13.50367656449418 7.452207509930261 3 5.641525393902488 4.670041270661595 4 2.8321099672125554 3.125885171465571 5 1.7026420484762745 2.349071713873135 6 1.043232549159442 1.7271708338804315 7 0.7044035264324372 1.360574972764263 8 0.530703999728481 1.171508015150615 9 0.4245067999614137 1.0542171507225624 >10 6.13733569893061 43.23549759099924 >50 0.529443310412764 8.783830479250037 >100 0.07129555273081384 3.6314290536552973 >500 0.0029530702314538274 0.5664358820068816 >1k 0.002531203055531852 1.8160637474700696 >5k 4.2186717592197537E-4 0.6035696769843159 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC 5185 0.5974190574951032 TruSeq Adapter, Index 21 (95% over 23bp) TCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC 4121 0.47482428851250147 TruSeq Adapter, Index 21 (95% over 23bp) CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT 4063 0.46814149095517915 Illumina Paired End PCR Primer 2 (95% over 21bp) GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC 3374 0.3887544648000922 TruSeq Adapter, Index 21 (95% over 23bp) TCCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTG 1655 0.1906901716787648 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1273 0.14667588431846987 No Hit CGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTG 1115 0.1284710220071437 No Hit GCCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTG 1000 0.11522064754003918 TruSeq Adapter, Index 21 (95% over 22bp) TTCCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCT 915 0.10542689249913584 TruSeq Adapter, Index 21 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.4879594423320659 0.0 2 0.0 0.0 0.0 2.046779582901256 0.0 3 0.0 0.0 0.0 2.889042516418942 0.0 4 0.0 0.0 0.0 3.7481276644774746 0.0 5 0.0 0.0 0.0 5.150132503744671 0.0 6 0.0 0.0 0.0 5.899527595345086 0.0 7 0.0 0.0 0.0 6.628182970388294 0.0 8 0.0 0.0 0.0 7.711487498559742 0.0 9 0.0 0.0 0.0 8.20486231132619 0.0 10 0.0 0.0 0.0 9.279294849637054 0.0 11 0.0 0.0 0.0 11.160963244613434 0.0 12 0.0 0.0 0.0 13.138840880285747 0.0 13 0.0 0.0 0.0 13.718631178707223 0.0 14 0.0 0.0 0.0 13.930291508238277 0.0 15 0.0 0.0 0.0 14.248415716096325 0.0 16 0.0 0.0 0.0 14.976379767254292 0.0 17 0.0 0.0 0.0 16.122709989630142 0.0 18 0.0 0.0 0.0 17.319276414333448 0.0 19 0.0 0.0 0.0 18.20831893075239 0.0 20 0.0 0.0 0.0 19.110035718400738 0.0 21 0.0 0.0 0.0 20.286092867841916 0.0 22 0.0 0.0 0.0 21.627606867150593 0.0 23 0.0 0.0 0.0 22.803087913354073 0.0 24 0.0 0.0 0.0 23.696393593731997 0.0 25 0.0 0.0 0.0 24.455928102315934 0.0 26 0.0 0.0 0.0 25.187118331605024 0.0 27 0.0 0.0 0.0 25.87775089296002 0.0 28 0.0 0.0 0.0 26.609056342896647 0.0 29 0.0 0.0 0.0 27.380228136882128 0.0 30 0.0 0.0 0.0 28.216384376080192 0.0 31 0.0 0.0 0.0 29.02327457080309 0.0 32 0.0 0.0 0.0 29.759764949879017 0.0 33 0.0 0.0 0.0 30.47447862656988 0.0 34 0.0 0.0 0.0 31.217536582555596 0.0 35 0.0 0.0 0.0 31.956561815877404 0.0 36 1.1522064754003918E-4 0.0 0.0 32.67911049660099 0.0 37 1.1522064754003918E-4 0.0 0.0 33.38552828666897 0.0 38 1.1522064754003918E-4 0.0 0.0 34.05588201405692 0.0 39 1.1522064754003918E-4 0.0 0.0 34.750316856780735 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACGAC 20 7.0323306E-4 45.0 26 CTATCGG 45 3.8380676E-10 45.0 2 GAGTCGT 35 1.2114651E-7 45.0 9 CGTTGCG 20 7.0323306E-4 45.0 2 ATTCGAA 20 7.0323306E-4 45.0 10 TAAGTCG 20 7.0323306E-4 45.0 10 CACGACG 20 7.0323306E-4 45.0 15 CGACAGG 20 7.0323306E-4 45.0 38 CCCGTAA 20 7.0323306E-4 45.0 16 CCGTACT 20 7.0323306E-4 45.0 22 TATCTCG 20 7.0323306E-4 45.0 1 GGGCGTA 25 3.890013E-5 45.0 7 TCCGTAC 20 7.0323306E-4 45.0 21 TTCGGTA 20 7.0323306E-4 45.0 40 AACTCGA 20 7.0323306E-4 45.0 10 CCGCGCA 20 7.0323306E-4 45.0 25 CGGTAAT 20 7.0323306E-4 45.0 42 CGTTTCG 20 7.0323306E-4 45.0 13 CCGCAAT 20 7.0323306E-4 45.0 15 TGCGTCG 20 7.0323306E-4 45.0 2 >>END_MODULE